##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920896_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3369543
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.283506398345413	31.0	30.0	34.0	27.0	34.0
2	30.301074656118054	31.0	30.0	34.0	26.0	34.0
3	30.44056419520392	31.0	30.0	34.0	27.0	34.0
4	34.08380186868071	35.0	35.0	37.0	30.0	37.0
5	33.62043962638257	35.0	35.0	37.0	28.0	37.0
6	33.5636580390872	35.0	35.0	37.0	28.0	37.0
7	33.37461578617634	35.0	35.0	37.0	27.0	37.0
8	33.30295413947826	35.0	33.0	37.0	27.0	37.0
9	34.64524477058165	38.0	35.0	39.0	27.0	39.0
10-11	34.45286601180041	38.0	34.0	39.0	27.0	39.0
12-13	34.27147197706039	37.0	34.0	39.0	27.0	39.0
14-15	35.17297152759291	38.0	34.0	40.0	26.0	41.0
16-17	35.04123452349472	38.0	34.0	40.0	25.5	41.0
18-19	34.94368138349918	38.5	34.0	40.0	25.0	41.0
20-21	34.81745788672232	38.0	34.0	40.0	25.0	41.0
22-23	34.66609537257723	38.0	34.0	40.0	24.5	41.0
24-25	34.478893428574736	38.0	33.0	40.0	24.0	41.0
26-27	34.2981929596981	38.0	33.0	40.0	23.0	41.0
28-29	34.236098485759044	38.0	33.0	40.0	23.5	41.0
30-31	34.221737487843306	38.0	33.0	40.0	23.0	41.0
32-33	34.073930055203334	38.0	33.0	40.0	22.0	41.0
34-35	33.90116270960186	38.0	33.0	40.0	21.5	41.0
36-37	33.70065955531655	38.0	33.0	40.0	19.5	41.0
38-39	33.507727309014896	38.0	32.0	40.0	18.5	41.0
40-41	33.27390568988139	38.0	32.0	40.0	17.0	41.0
42-43	33.28888413057794	38.0	32.0	40.0	17.0	41.0
44-45	33.14096421977699	38.0	32.0	40.0	15.0	41.0
46-47	32.88520045596688	37.5	31.5	40.0	14.5	41.0
48-49	32.61656966538192	37.0	31.0	40.0	11.5	41.0
50-51	32.34883380327837	37.0	31.0	40.0	9.0	41.0
52-53	32.07549109775421	36.5	31.0	40.0	8.0	41.0
54-55	31.775350099405173	36.0	30.0	39.5	7.0	41.0
56-57	31.43525739247132	36.0	30.0	39.0	4.0	41.0
58-59	31.088627152109353	35.0	29.0	39.0	2.0	40.0
60-61	30.739213448233187	35.0	29.0	39.0	2.0	40.0
62-63	30.40524694298307	35.0	28.5	38.5	2.0	40.0
64-65	29.993794262307976	34.5	28.0	38.0	2.0	40.0
66-67	29.54538033792713	34.0	28.0	37.5	2.0	40.0
68-69	29.0866086291227	34.0	27.5	37.0	2.0	39.0
70-71	28.658507103188768	34.0	26.0	36.0	2.0	39.0
72-73	28.218379465702025	33.5	26.0	36.0	2.0	39.0
74-75	27.783249390199206	33.0	26.0	35.0	2.0	38.0
76-77	26.352647970362746	31.5	24.0	34.0	2.0	36.0
78-79	27.197977144081555	33.0	26.0	35.0	2.0	36.5
80-81	27.231120807777195	33.0	26.0	35.0	2.0	36.0
82-83	26.905689881387474	33.0	25.0	35.0	2.0	36.0
84-85	26.59586863856612	33.0	25.0	35.0	2.0	35.5
86-87	26.0691351913301	32.0	23.5	35.0	2.0	35.0
88-89	25.708230908464444	32.0	22.0	35.0	2.0	35.0
90-91	25.33750467051467	32.0	20.0	34.5	2.0	35.0
92-93	25.029519433347488	32.0	19.0	34.0	2.0	35.0
94-95	24.646196680083918	32.0	17.0	34.0	2.0	35.0
96-97	24.102637360615372	31.0	4.5	34.0	2.0	35.0
98-99	23.490376588160473	31.0	2.0	34.0	2.0	35.0
100-101	21.305742054634706	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	76508.0
3	35114.0
4	19472.0
5	13799.0
6	12207.0
7	12020.0
8	13729.0
9	15536.0
10	17734.0
11	19939.0
12	21912.0
13	23708.0
14	25166.0
15	27186.0
16	28399.0
17	29576.0
18	29087.0
19	28090.0
20	28515.0
21	29818.0
22	32283.0
23	36535.0
24	42371.0
25	50141.0
26	58822.0
27	70608.0
28	83252.0
29	96967.0
30	114819.0
31	136143.0
32	164112.0
33	199293.0
34	250843.0
35	325381.0
36	430054.0
37	489719.0
38	263332.0
39	17353.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.181072092945804	18.64509341681455	12.782278399710734	25.391556090528912
2	16.71490762990708	20.271680759082166	37.11601246815963	25.897399142851125
3	19.55099808050596	23.806534471791867	28.60954368755968	28.03292376014249
4	13.9313471041215	15.740069051819733	34.89111515839414	35.437468685664626
5	14.953818300537202	36.609117620870805	33.57810227643724	14.858961802154749
6	35.03147737164689	34.32771523896955	16.345921311027332	14.294886078356225
7	30.394802812577364	30.792247728875576	20.280835713158478	18.53211374538858
8	27.563151509244634	33.83211122498373	19.565431206596383	19.039306059175264
9	27.25770509420986	14.949853212241607	18.782562125489118	39.00987956805941
10-11	26.3654049961925	24.73881540459509	27.000662264838788	21.895117334373627
12-13	26.837265457488535	21.935261416396624	27.017704772104373	24.209768354010468
14-15	23.91725710828922	23.58696238342266	24.186805414319842	28.308975093968275
16-17	24.42539825180957	26.538598088890232	24.98853313349036	24.047470525809842
18-19	24.501912246500922	25.656121156516704	26.216481481288973	23.6254851156934
20-21	25.12042077093341	25.026159389019988	25.815416619721532	24.03800322032507
22-23	24.74155962425306	24.82866184260473	25.87829926569785	24.551479267444353
24-25	24.576757751689666	25.16215903619323	25.78164983447433	24.479433377642774
26-27	24.33690064920853	25.362817020364197	25.774448665723686	24.52583366470359
28-29	24.325820021040776	25.450107720053445	25.815805166020112	24.408267092885666
30-31	24.57784938400422	25.25050593954848	25.639361391858806	24.53228328458849
32-33	24.23240983582954	25.337373928689804	25.536855564400625	24.893360671080032
34-35	24.741128585566486	25.26232956370327	25.614942054479062	24.381599796251187
36-37	24.827897489969807	25.046438475087378	25.551810792546515	24.5738532423963
38-39	24.672786438870585	25.074495755578813	25.621211106907104	24.6315066986435
40-41	24.688898391359672	25.02052340737097	25.815915833769825	24.47466236749953
42-43	24.701267235096307	24.96863782726963	25.635980934410945	24.69411400322311
44-45	24.386519799817698	25.066544124249933	25.77708531021295	24.76985076571941
46-47	24.551263610678415	24.974090095514107	25.692323632296965	24.782322661510513
48-49	24.457446341205706	25.1303850231446	25.496801663989515	24.915366971660177
50-51	24.30413524377584	25.245816485617883	25.674441660093304	24.775606610512973
52-53	24.533062378601265	25.001702865607474	25.784323575035383	24.68091118075588
54-55	24.248422999095464	24.93907556321991	25.839010487120945	24.973490950563683
56-57	24.09684538996231	25.084670860625152	25.67170930000129	25.146774449411247
58-59	24.03453538211689	24.893453282974427	25.78445391394468	25.287557420964003
60-61	23.995636396019325	25.023799452323175	25.879600151390342	25.10096400026716
62-63	23.82387445044243	25.11359192683696	25.836511028252364	25.22602259446825
64-65	23.753948516948462	24.930037458785986	25.704069951559717	25.61194407270584
66-67	23.799425771179273	25.033434332803022	25.704110199917196	25.46302969610051
68-69	24.027813959318713	24.807122269910895	25.657943788093963	25.50711998267643
70-71	23.70296621766432	24.786971748413826	25.687380855271396	25.822681178650452
72-73	23.799078178738668	24.852267951383112	25.57272380561023	25.77593006426799
74-75	23.731270643005058	24.90990556811436	25.454845999970594	25.903977788909987
76-77	23.75479480075125	24.715443405173215	25.641579113747515	25.888182680328022
78-79	23.62690547105276	24.893996941979587	25.576601391672536	25.902496195295114
80-81	23.55224044959929	24.982476022612943	25.48500566421848	25.98027786356929
82-83	23.495293230179307	24.978032204987834	25.48875654043699	26.037918024395868
84-85	23.34748799416341	24.934681926769255	25.45544561655438	26.262384462512955
86-87	23.277667588271168	24.95312999116682	25.460818368728788	26.308384051833222
88-89	23.203247463180695	24.90481794836801	25.36196653927945	26.529968049171842
90-91	23.107784719800318	24.94622875187619	25.345019776630608	26.60096675169289
92-93	23.155786314577654	24.840177603108547	25.32574641984578	26.678289662468025
94-95	22.875602555570147	24.74026952050043	25.485260806305977	26.89886711762345
96-97	23.009597701430863	24.67002661155894	25.395793702767005	26.92458198424319
98-99	22.753665792284952	24.71971136945034	25.363485231991124	27.163137606273587
100-101	22.92651271512911	24.65640481371603	25.385947292255057	27.031135178899806
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	49.0
1	37.0
2	53.5
3	87.5
4	79.0
5	82.0
6	160.5
7	311.5
8	472.0
9	505.5
10	407.5
11	344.5
12	345.0
13	393.5
14	453.5
15	507.5
16	575.5
17	637.0
18	709.0
19	787.0
20	918.0
21	1074.0
22	1295.5
23	1615.5
24	1923.5
25	2389.0
26	3164.0
27	4193.0
28	5311.0
29	7204.0
30	10037.0
31	14082.0
32	19634.5
33	25107.5
34	30375.5
35	36670.5
36	44773.5
37	53398.5
38	61596.5
39	69487.5
40	77282.0
41	85180.0
42	92955.0
43	102797.0
44	114177.5
45	123849.0
46	131123.5
47	138225.5
48	146082.5
49	152148.0
50	153236.5
51	151752.0
52	151119.5
53	152989.5
54	151951.5
55	144305.0
56	135028.5
57	123807.0
58	113829.5
59	102532.5
60	87408.0
61	73441.0
62	60849.0
63	47349.5
64	35167.5
65	27111.5
66	20845.5
67	15675.5
68	12020.0
69	9453.0
70	7597.0
71	6144.0
72	5101.0
73	4502.5
74	3595.0
75	2708.0
76	2013.5
77	1287.0
78	738.5
79	394.0
80	224.0
81	143.5
82	79.5
83	36.0
84	22.5
85	16.0
86	10.0
87	7.5
88	3.5
89	1.0
90	2.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1944180560984086
2	0.0
3	0.01264266400517815
4	0.014215577602066511
5	0.007211660453657959
6	0.10781877542444182
7	0.1424525521710214
8	0.12417114130907367
9	0.14307578208676963
10-11	0.11336848943610453
12-13	0.11480785376533256
14-15	0.11611366882690027
16-17	0.06625527556704276
18-19	0.0798773008683967
20-21	0.037082179987019015
22-23	0.08336442063508315
24-25	0.05753005674656771
26-27	0.06564688445881237
28-29	0.039218968269584334
30-31	0.02405370698637768
32-33	0.0315324659753563
34-35	0.0204478767595487
36-37	0.0248846802073753
38-39	0.0315324659753563
40-41	0.02458790405701901
42-43	0.01295427896305226
44-45	0.027110501335047513
46-47	0.033832481140617585
48-49	0.008636185975368173
50-51	0.015298810550866987
52-53	0.006603269345427555
54-55	0.012182660972125893
56-57	0.005119388593646082
58-59	0.0038284123395962003
60-61	0.022792408347363426
62-63	0.010550392145166274
64-65	0.007122627608551071
66-67	0.04076220425143707
68-69	0.08998252878802852
70-71	0.02199111274140143
72-73	0.045599655502244665
74-75	0.0871483165521259
76-77	0.2516810143096556
78-79	0.07891277837973874
80-81	0.01743559883343231
82-83	0.012063950511983376
84-85	0.013429120803622331
86-87	0.26467980969526134
88-89	0.6220428111467935
90-91	0.26742498908605705
92-93	0.35113070229404997
94-95	0.0012019434089429932
96-97	0.004229060142577198
98-99	0.0033980869215795733
100-101	0.005074872171092638
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3369543.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.70019091927083
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.81953796475054	33.781054817331494
2	13.107823104072164	12.50491329200543
3	5.762221595498622	8.245772106732892
4	3.1475500280688626	6.005549490673641
5	1.8655829756276874	4.449433205659103
6	1.2068614849799457	3.454051394799487
7	0.8292669345761079	2.768933377162317
8	0.593091560236887	2.263246452472618
9	0.4369267182097429	1.8757339088701661
>10	2.095415631069736	16.922616590446754
>50	0.09152976763172764	2.93919277991884
>100	0.04137912208222405	3.648772178863429
>500	0.002000436052228536	0.6262909613229439
>1k	8.12677143641912E-4	0.5144394437409798
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0774330524940622E-4
2	0.0	0.0	0.0	0.0	2.0774330524940622E-4
3	0.0	0.0	0.0	0.0	2.0774330524940622E-4
4	0.0	0.0	0.0	0.0	2.0774330524940622E-4
5	0.0	0.0	0.0	0.0	2.0774330524940622E-4
6	0.0	0.0	0.0	0.0	2.374209202850357E-4
7	0.0	0.0	0.0	2.967761503562946E-5	2.374209202850357E-4
8	0.0	0.0	0.0	2.967761503562946E-5	2.374209202850357E-4
9	0.0	0.0	0.0	2.967761503562946E-5	2.374209202850357E-4
10-11	0.0	0.0	0.0	2.967761503562946E-5	2.6709853532066514E-4
12-13	0.0	0.0	0.0	2.967761503562946E-5	7.567791834085512E-4
14-15	0.0	0.0	0.0	8.903284510688838E-5	8.903284510688839E-4
16-17	0.0	0.0	0.0	1.1871046014251785E-4	0.001053555333764846
18-19	0.0	0.0	0.0	1.6322688269596204E-4	0.0011871046014251783
20-21	0.0	0.0	0.0	2.2258211276722094E-4	0.0014393643292280288
22-23	0.0	0.0	0.0	2.967761503562946E-4	0.001528397174334917
24-25	0.0	0.0	0.0	6.825851458194777E-4	0.0015729135968883614
26-27	0.0	0.0	0.0	0.0014096867141923995	0.0016322688269596204
28-29	0.0	0.0	0.0	0.005831651354501189	0.0016322688269596204
30-31	0.0	0.0	0.0	0.02657630426440618	0.0016767852495130646
32-33	0.0	0.0	0.0	0.07012820432919242	0.0017509792871021382
34-35	0.0	0.0	0.0	0.1296318224756295	0.0018993673622802855
36-37	0.0	0.0	0.0	0.22973441799080765	0.0018993673622802855
38-39	0.0	0.0	0.0	0.39716068321431125	0.0019735613998693594
40-41	0.0	0.0	0.0	0.5906290556315797	0.0021219494750475063
42-43	0.0	0.0	0.0	0.7896768196755465	0.0023296927802969125
44-45	0.0	0.0	0.0	1.0333003615030287	0.0024929196629928747
46-47	0.0	0.0	0.0	1.3227609797530406	0.002596791315617578
48-49	0.0	0.0	0.0	1.6321797941145135	0.002700662968242281
50-51	0.0	0.0	0.0	1.9745407611655348	0.0028787286584560576
52-53	0.0	0.0	0.0	2.322525636265808	0.003027116733634205
54-55	0.0	0.0	0.0	2.7046546074645734	0.0031161495787410936
56-57	0.0	0.0	0.0	3.1549085439776254	0.003234860038883611
58-59	0.0	0.0	0.0	3.6325104027460102	0.0033090540764726846
60-61	0.0	0.0	0.0	4.145487978636865	0.0033387316915083143
62-63	0.0	0.0	0.0	4.665513988098683	0.0035316361892399058
64-65	0.0	0.0	0.0	5.217651177029051	0.0035761526117933503
66-67	1.483880751781473E-5	0.0	0.0	5.845303057417579	0.0035909914193111648
68-69	2.967761503562946E-5	0.0	0.0	6.53512954130575	0.003605830226828979
70-71	2.967761503562946E-5	0.0	0.0	7.2642046710785415	0.0036651854569002386
72-73	2.967761503562946E-5	0.0	0.0	8.009246357740501	0.003872928762149645
74-75	2.967761503562946E-5	0.0	0.0	8.766366833721962	0.004080672067399051
76-77	2.967761503562946E-5	0.0	0.0	9.580379297726724	0.004154866104988124
78-79	2.967761503562946E-5	0.0	0.0	10.469431611349076	0.004199382527541569
80-81	2.967761503562946E-5	0.0	0.0	11.398889997842439	0.0042884153726484575
82-83	4.451642255344419E-5	0.0	0.0	12.345323980136179	0.004540675100451307
84-85	5.935523007125892E-5	0.0	0.0	13.282157253965893	0.004540675100451307
86-87	5.935523007125892E-5	0.0	0.0	14.24967718174245	0.004644546753076011
88-89	5.935523007125892E-5	0.0	0.0	15.277917509881904	0.004748418405700713
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4895	0.0	44.490826	1
GGTATCA	2660	0.0	42.90296	1
TCAACGC	6415	0.0	33.74545	4
ATCAACG	6475	0.0	33.432747	3
CAACGCA	6530	0.0	32.927185	5
AACGCAG	6800	0.0	31.899458	6
TATCAAC	6850	0.0	31.738264	2
GTACATG	11510	0.0	26.935804	1
TACATGG	11775	0.0	25.719828	2
ACGCAGA	8485	0.0	25.229038	7
ACATGGG	12220	0.0	24.474644	3
CGCAGAG	8975	0.0	23.796623	8
GAGTACA	8775	0.0	20.971115	1
CATGGGG	7935	0.0	20.819931	4
AGTACAT	8535	0.0	20.021992	2
GCAGAGT	10415	0.0	19.367205	9
TAAGGCG	970	0.0	18.594368	5
GTATACG	205	1.598678E-5	18.526062	4
GGACCGT	470	0.0	18.17819	6
GTATTAA	1740	0.0	18.036505	1
>>END_MODULE