FastQCFastQC Report
Mon 15 Aug 2016
SRR2920895_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920895_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3001686
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46650.046.961121
GGTATCA24800.041.0132061
TCAACGC60450.035.2725874
TATCAAC62450.034.4383852
CAACGCA61700.034.4093555
ATCAACG63600.033.5976943
AACGCAG65500.032.47936
GTACATG101300.026.3058431
ACGCAGA80450.026.0404117
CGCAGAG84050.024.7064848
TACATGG106350.024.6638552
TAAGGTG13500.024.1159975
ACATGGG106500.023.7237133
GTAAGGT12100.022.358744
AGGTAAG12950.021.6004332
GCAGAGT92800.021.289499
CATGGGG79250.021.1769034
AAGGTAA13150.020.2995011
TAAGGCG10850.020.28585
TAGTACG1606.549272E-520.0612854
Produced by FastQC (version 0.11.5)