##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2920895_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3001686 Sequences flagged as poor quality 0 Sequence length 98 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.376369480352043 31.0 28.0 33.0 18.0 34.0 2 28.38632555170661 31.0 28.0 33.0 16.0 34.0 3 28.460031462318177 31.0 28.0 34.0 16.0 34.0 4 31.919997294853626 35.0 32.0 37.0 20.0 37.0 5 31.8577132984596 35.0 32.0 37.0 19.0 37.0 6 31.983992329644074 35.0 32.0 37.0 22.0 37.0 7 31.810355913310055 35.0 32.0 37.0 19.0 37.0 8 31.74622528805478 35.0 32.0 37.0 18.0 37.0 9 32.98986036514146 37.0 32.0 39.0 18.0 39.0 10-11 32.7888125206967 37.0 32.0 39.0 17.0 39.0 12-13 32.60584451538236 37.0 32.0 39.0 17.0 39.0 14-15 33.34271606024082 38.0 32.0 40.0 17.0 41.0 16-17 33.222957198054694 38.0 32.0 40.0 16.0 41.0 18-19 33.08888987722233 38.0 32.0 40.0 13.0 41.0 20-21 32.98529093316223 38.0 32.0 40.0 10.5 41.0 22-23 32.76018710817854 38.0 31.5 40.0 10.0 41.0 24-25 32.553227919242715 37.5 31.0 40.0 10.0 41.0 26-27 32.57193523906231 38.0 31.0 40.0 9.5 41.0 28-29 32.4036166674329 38.0 31.0 40.0 8.5 41.0 30-31 32.14134456435483 37.0 30.5 40.0 8.0 41.0 32-33 31.882688095956738 37.0 30.0 40.0 4.5 41.0 34-35 31.547068880622426 37.0 30.0 40.0 2.0 41.0 36-37 31.2364151346943 37.0 30.0 40.0 2.0 41.0 38-39 31.018230754316075 36.0 29.5 40.0 2.0 41.0 40-41 30.798659153555704 36.0 29.0 39.5 2.0 41.0 42-43 30.539617734833023 36.0 28.5 39.0 2.0 40.5 44-45 30.23943377155372 35.5 28.0 39.0 2.0 40.0 46-47 29.979214514776032 35.0 27.0 39.0 2.0 40.0 48-49 29.65233105661285 35.0 26.5 39.0 2.0 40.0 50-51 28.49846269729745 34.0 25.0 38.0 2.0 39.5 52-53 28.938183074445497 34.5 26.5 38.0 2.0 39.5 54-55 29.56423190167126 35.0 27.0 39.0 2.0 40.0 56-57 29.494552061741302 35.0 27.0 39.0 2.0 40.0 58-59 29.231639651848994 35.0 26.5 39.0 2.0 40.0 60-61 28.883488146328432 34.5 26.0 38.5 2.0 40.0 62-63 28.476850676586423 34.0 26.0 38.0 2.0 40.0 64-65 28.035230866919456 34.0 25.0 37.5 2.0 40.0 66-67 27.63404633262773 34.0 24.5 37.0 2.0 39.5 68-69 27.179078024816718 33.0 23.0 36.0 2.0 39.0 70-71 26.838362840083875 33.0 22.0 36.0 2.0 39.0 72-73 26.379276679839265 33.0 21.5 35.5 2.0 38.5 74-75 25.943069161797737 32.0 20.0 35.0 2.0 37.5 76-77 25.53415796988759 32.0 18.5 35.0 2.0 37.0 78-79 25.08566868753094 32.0 17.5 35.0 2.0 36.0 80-81 24.65113039804963 31.5 11.5 35.0 2.0 36.0 82-83 24.189660577422153 31.0 7.0 34.0 2.0 35.5 84-85 23.795853063911416 31.0 2.0 34.0 2.0 35.0 86-87 23.3453499133487 31.0 2.0 34.0 2.0 35.0 88-89 22.81000627647262 30.5 2.0 34.0 2.0 35.0 90-91 22.304725577558745 30.0 2.0 34.0 2.0 35.0 92-93 21.760321532631995 30.0 2.0 34.0 2.0 35.0 94-95 21.104322370827596 29.0 2.0 34.0 2.0 35.0 96-97 20.447078575174086 29.0 2.0 34.0 2.0 35.0 98 20.040725445632887 29.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 170089.0 3 28386.0 4 20993.0 5 17047.0 6 16167.0 7 16869.0 8 18840.0 9 21647.0 10 23487.0 11 25008.0 12 25803.0 13 25020.0 14 23649.0 15 24802.0 16 26263.0 17 28428.0 18 29027.0 19 29200.0 20 30291.0 21 31674.0 22 34887.0 23 38698.0 24 44582.0 25 50644.0 26 58158.0 27 67270.0 28 77652.0 29 89613.0 30 103830.0 31 121929.0 32 144179.0 33 174029.0 34 215182.0 35 270561.0 36 336419.0 37 344740.0 38 180893.0 39 15730.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.90010912631061 17.16557200346609 12.739685757695446 25.194633112527853 2 16.6346656252166 19.098023821837394 38.04152959868628 26.225780954259726 3 19.837333194717676 22.71004270428482 28.271596052652626 29.181028048344874 4 14.018863171699778 14.97391703029996 34.63590466721222 36.371315130788034 5 15.35422313361666 35.954999270247626 33.20516571376779 15.485611882367916 6 35.25739667645226 33.20638718298173 16.607575449598635 14.928640690967374 7 30.587073031358653 30.645182249366513 20.001599336275447 18.76614538299939 8 27.841340707669755 33.11654561204771 19.508695941893784 19.533417738388753 9 27.534425652783135 15.288674431636087 18.74063443011694 38.436265485463835 10-11 26.566357468162472 24.45561002310202 26.690121266868232 22.287911241867278 12-13 27.220035928367153 21.81627157900909 26.49100724697554 24.472685245648215 14-15 24.180989405909354 23.340331871991374 24.040413395749372 28.4382653263499 16-17 24.54947436176784 26.241181891418076 24.852706685404 24.356637061410087 18-19 24.644863841907608 25.4212818245306 26.13042935611164 23.803424977450153 20-21 25.284375730381438 24.804211931561113 25.820952899170436 24.090459438887017 22-23 24.99341387854192 24.45448573229334 25.883874173400073 24.668226215764673 24-25 24.838919265615864 24.679255321631423 25.758016200081475 24.72380921267124 26-27 24.414141257547804 24.976474967448357 25.781702033813037 24.8276817411908 28-29 24.63892755617423 25.200269870984606 25.605076347888232 24.55572622495293 30-31 24.771597816165304 24.971738776465262 25.34020546738639 24.916457939983044 32-33 24.51711093765489 24.997005242283812 25.303095304936313 25.182788515124983 34-35 25.0545226013064 25.032827219180188 25.305373367832 24.60727681168141 36-37 25.170560569263063 24.68973917260207 25.296848397005263 24.842851861129606 38-39 24.84912765431475 24.811686776005637 25.312276507497465 25.026909062182153 40-41 25.050512760382798 24.715942615562533 25.54784856901296 24.68569605504171 42-43 24.9911833042901 24.653639371381306 25.300888562319816 25.054288762008774 44-45 24.656217965261277 24.73350693384576 25.560202854690882 25.050072246202078 46-47 24.96904995659802 24.781192141147585 25.2807748465878 24.968983055666595 48-49 24.760933828089097 24.89594475539492 25.11623014304168 25.226891273474305 50-51 24.54606858107436 25.086771108059736 25.4419661618181 24.925194149047805 52-53 24.986278520596944 24.6247342718379 25.473675509100353 24.9153116984648 54-55 24.41723209845307 24.604967499804214 25.706931969469693 25.27086843227302 56-57 24.38955273067317 24.84804686650507 25.62732630402671 25.135074098795045 58-59 24.489803769265226 24.469431713303287 25.582334717897204 25.458429799534287 60-61 24.14545710034302 24.434992877868194 26.015227895671433 25.404322126117357 62-63 24.266433721051975 24.564339184068178 25.930419372351388 25.238807722528456 64-65 24.15202590090241 24.56870874336179 25.686528532090318 25.592736823645485 66-67 24.147826744800263 24.554724201486128 25.694291485263587 25.603157568450026 68-69 24.431473947511307 24.249166242079344 25.808257590634483 25.511102219774873 70-71 24.100074988526003 24.29145038844391 25.828272095490405 25.780202527539682 72-73 24.21027326818478 24.300128924059184 25.776937973351238 25.712659834404803 74-75 24.18425673753507 24.47241487551201 25.785490000879186 25.55783838607374 76-77 24.254546530326333 24.177210344897322 25.904596864867592 25.663646259908752 78-79 24.236207169737906 24.30177588769314 25.862989960281034 25.59902698228792 80-81 24.360770534019494 24.222825319265777 25.87139768157758 25.545006465137156 82-83 24.274943934569603 24.22100143490058 25.91842663753181 25.585627992998006 84-85 24.155814240769818 24.172429915368713 26.02043059336847 25.651325250492995 86-87 24.325583696346868 24.136706350798097 25.944921554312977 25.59278839854206 88-89 24.191480915816097 24.00365387387213 25.903327178888997 25.90153803142278 90-91 24.16191563287381 24.07481891907796 25.93628131469564 25.826984133352592 92-93 24.421250541787554 23.86959255068649 25.95859832100601 25.75055858651994 94-95 24.260205173331478 23.69424666803966 26.13942278116717 25.90612537746169 96-97 24.551893195249257 23.403492043538208 26.12494767685224 25.91966708436029 98 24.535718655701995 23.36999219706457 26.017741481461176 26.07654766577226 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 45.0 1 49.5 2 68.0 3 99.5 4 121.0 5 147.0 6 223.5 7 442.5 8 677.0 9 764.0 10 810.5 11 885.0 12 998.0 13 1156.0 14 1319.0 15 1468.5 16 1599.5 17 1699.0 18 1773.0 19 1777.5 20 1781.5 21 1799.0 22 1888.5 23 2146.0 24 2508.5 25 2952.5 26 3596.0 27 4522.0 28 5811.5 29 7751.0 30 10775.0 31 15307.0 32 19211.0 33 23177.0 34 28527.0 35 34189.0 36 40734.5 37 47693.5 38 54600.5 39 61651.0 40 69022.5 41 76351.0 42 84995.5 43 93745.5 44 101673.5 45 110339.5 46 119382.0 47 126487.0 48 132196.5 49 136522.5 50 137612.0 51 139436.0 52 142241.0 53 145646.0 54 145293.0 55 141471.5 56 133358.0 57 121301.5 58 111584.0 59 97888.0 60 82111.0 61 68787.5 62 56832.0 63 44009.0 64 32037.0 65 23985.0 66 18429.0 67 14369.5 68 10882.5 69 8285.0 70 6311.5 71 4911.0 72 3998.0 73 3205.0 74 2484.0 75 1859.0 76 1315.0 77 865.5 78 538.0 79 313.5 80 184.5 81 111.0 82 60.0 83 30.5 84 19.5 85 18.5 86 14.5 87 9.0 88 6.5 89 7.5 90 8.0 91 7.0 92 5.5 93 4.0 94 5.0 95 4.0 96 2.5 97 2.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3855499875736503 2 0.024719441007487124 3 0.01938910332393195 4 0.016457417597976604 5 0.022254159828842858 6 0.022520676713020615 7 0.014691743240298951 8 0.009527978609354875 9 0.0 10-11 1.665730526110992E-5 12-13 0.005263708462510735 14-15 0.0035646633258775233 16-17 0.00639640522026621 18-19 0.03702918959544736 20-21 0.07985512142176097 22-23 0.09847798870368187 24-25 0.06489686129728427 26-27 0.07400840727511139 28-29 0.05776753464552921 30-31 0.05165430361470187 32-33 0.020271940502770775 34-35 0.34200779162110895 36-37 0.5213736546727405 38-39 0.4767154192677049 40-41 0.48537721800348205 42-43 0.43435589198870234 44-45 0.40828720925506534 46-47 0.4061384168763821 48-49 0.39737667430903834 50-51 0.39242945464648865 52-53 0.4554107258387453 54-55 0.4565434225965007 56-57 0.4254608909792697 58-59 0.4096697655917374 60-61 0.425211031400353 62-63 0.40467257401340445 64-65 0.4558105011650119 66-67 0.4771984811202771 68-69 0.41673246302244804 70-71 0.37407310424874557 72-73 0.3590648722084855 74-75 0.3422409938947645 76-77 0.33985899924242574 78-79 0.33874295978993135 80-81 0.3545840570932469 82-83 0.3510693656831527 84-85 0.35095276454632496 86-87 0.3352116110745761 88-89 0.38095257132158394 90-91 0.3583985799980411 92-93 0.34748804505201414 94-95 0.3549338605037302 96-97 0.35506711894581916 98 0.34993666892539727 >>END_MODULE >>Sequence Length Distribution pass #Length Count 98 3001686.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.809223390121986 #Duplication Level Percentage of deduplicated Percentage of total 1 75.41852410608473 40.58212211377614 2 11.715237390923514 12.607756516730264 3 4.90859874468276 7.92383659155321 4 2.5065985303445903 5.395124810746541 5 1.4695138805530588 3.9536700336782284 6 0.9216845733955625 2.975707866304266 7 0.6413934569955965 2.4159018665907057 8 0.44058376434589175 1.8965976158199158 9 0.3410180785453147 1.6514926171663502 >10 1.5388968219097343 14.161905630983021 >50 0.06877188115301497 2.514553936294083 >100 0.02649411355120298 2.4746541388494414 >500 0.0015608473928103413 0.5661462162340539 >1k 0.0011238101223834708 0.8805300452736898 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.3325844208887937E-4 2 0.0 0.0 0.0 0.0 1.3325844208887937E-4 3 0.0 0.0 0.0 0.0 1.3325844208887937E-4 4 0.0 0.0 0.0 0.0 1.3325844208887937E-4 5 0.0 0.0 0.0 0.0 1.3325844208887937E-4 6 0.0 0.0 0.0 0.0 1.6657305261109923E-4 7 0.0 0.0 0.0 0.0 1.9988766313331908E-4 8 0.0 3.331461052221984E-5 0.0 0.0 1.9988766313331908E-4 9 0.0 3.331461052221984E-5 0.0 0.0 1.9988766313331908E-4 10-11 0.0 3.331461052221984E-5 0.0 0.0 1.9988766313331908E-4 12-13 0.0 3.331461052221984E-5 0.0 1.665730526110992E-5 4.830618525721878E-4 14-15 0.0 3.331461052221984E-5 0.0 3.331461052221984E-5 6.163202946610671E-4 16-17 0.0 3.331461052221984E-5 0.0 3.331461052221984E-5 7.662360420110564E-4 18-19 0.0 3.331461052221984E-5 0.0 3.331461052221984E-5 8.328652630554961E-4 20-21 0.0 3.331461052221984E-5 0.0 6.662922104443968E-5 9.494663998832655E-4 22-23 0.0 3.331461052221984E-5 0.0 1.8323035787220913E-4 0.001099382147233255 24-25 0.0 4.9971915783329764E-5 0.0 5.830056841388473E-4 0.001099382147233255 26-27 0.0 6.662922104443968E-5 0.0 0.0015824439998054426 0.001099382147233255 28-29 0.0 6.662922104443968E-5 0.0 0.006796180546532849 0.001099382147233255 30-31 0.0 6.662922104443968E-5 0.0 0.026818261470386975 0.001116039452494365 32-33 0.0 6.662922104443968E-5 0.0 0.0718429575911671 0.0011326967577554748 34-35 0.0 9.994383156665954E-5 0.0 0.1364733020042736 0.0011326967577554748 36-37 0.0 1.3325844208887937E-4 0.0 0.24234713424388826 0.0011326967577554748 38-39 0.0 1.3325844208887937E-4 0.0 0.4131844570018316 0.0011660113682776946 40-41 0.0 1.3325844208887937E-4 0.0 0.6076418386200289 0.0011660113682776946 42-43 0.0 1.3325844208887937E-4 0.0 0.8132263001526475 0.0011993259787999144 44-45 0.0 1.3325844208887937E-4 0.0 1.061969839616802 0.0011993259787999144 46-47 0.0 1.3325844208887937E-4 0.0 1.3640167559165084 0.0012159832840610242 48-49 0.0 1.3325844208887937E-4 0.0 1.6828875505299354 0.0012659551998443542 50-51 0.0 1.3325844208887937E-4 0.0 2.0230297239618 0.0013658990314110136 52-53 0.0 1.3325844208887937E-4 0.0 2.375065213350097 0.0014325282524554534 54-55 0.0 1.3325844208887937E-4 0.0 2.7630971394076527 0.0014325282524554534 56-57 0.0 1.3325844208887937E-4 0.0 3.210029296868493 0.0014325282524554534 58-59 0.0 1.3325844208887937E-4 0.0 3.6856120193784427 0.0014325282524554534 60-61 0.0 1.3325844208887937E-4 0.0 4.1904782845374235 0.001482500168238783 62-63 0.0 1.3325844208887937E-4 0.0 4.71343438320997 0.0015657866945443329 64-65 0.0 1.3325844208887937E-4 0.0 5.261926130847797 0.0016657305261109923 66-67 0.0 1.3325844208887937E-4 0.0 5.895403449927807 0.0016657305261109923 68-69 0.0 1.3325844208887937E-4 0.0 6.598558276915041 0.0016657305261109923 70-71 0.0 1.3325844208887937E-4 0.0 7.323950606425855 0.0016990451366332123 72-73 0.0 1.3325844208887937E-4 0.0 8.075927995133402 0.0017656743576776519 74-75 0.0 1.3325844208887937E-4 0.0 8.839798699797381 0.0018156462734609815 76-77 0.0 1.3325844208887937E-4 0.0 9.672597333631831 0.0018323035787220915 78-79 0.0 1.3325844208887937E-4 1.665730526110992E-5 10.586567016003674 0.0018489608839832015 80-81 0.0 1.3325844208887937E-4 4.9971915783329764E-5 11.51957599828896 0.0018656181892443113 82-83 0.0 1.3325844208887937E-4 6.662922104443968E-5 12.471241162466693 0.0019988766313331907 84-85 0.0 1.3325844208887937E-4 6.662922104443968E-5 13.437281581084763 0.0019988766313331907 86 0.0 1.3325844208887937E-4 6.662922104443968E-5 14.179730991182955 0.0019988766313331907 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4665 0.0 46.96112 1 GGTATCA 2480 0.0 41.013206 1 TCAACGC 6045 0.0 35.272587 4 TATCAAC 6245 0.0 34.438385 2 CAACGCA 6170 0.0 34.409355 5 ATCAACG 6360 0.0 33.597694 3 AACGCAG 6550 0.0 32.4793 6 GTACATG 10130 0.0 26.305843 1 ACGCAGA 8045 0.0 26.040411 7 CGCAGAG 8405 0.0 24.706484 8 TACATGG 10635 0.0 24.663855 2 TAAGGTG 1350 0.0 24.115997 5 ACATGGG 10650 0.0 23.723713 3 GTAAGGT 1210 0.0 22.35874 4 AGGTAAG 1295 0.0 21.600433 2 GCAGAGT 9280 0.0 21.28949 9 CATGGGG 7925 0.0 21.176903 4 AAGGTAA 1315 0.0 20.299501 1 TAAGGCG 1085 0.0 20.2858 5 TAGTACG 160 6.549272E-5 20.061285 4 >>END_MODULE