FastQCFastQC Report
Mon 15 Aug 2016
SRR2920895_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920895_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3001686
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26450.046.392581
GTATCAA46950.043.2560421
TCAACGC59500.033.907794
CAACGCA60850.032.9176375
ATCAACG62450.032.3820763
TATCAAC62750.031.9976392
AACGCAG66100.030.8063226
GTACATG102200.026.6194841
TACATGG106300.025.0954132
ACGCAGA80500.024.8243267
ACATGGG107200.024.0011483
CGCAGAG83700.023.7598468
TAAGGTG15350.022.2638845
CATGGGG77650.020.846854
GCAGAGT94850.020.166149
GTAAGGT15400.019.1116644
TAAGGTA8500.018.9883634
AGAGTAC80850.018.57873510-11
CTATACT6500.018.2580414
AGGTAAG14600.018.20652