##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920895_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3001686
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.211916902700683	31.0	30.0	34.0	26.0	34.0
2	30.22712602184239	31.0	30.0	34.0	26.0	34.0
3	30.362886724327595	31.0	30.0	34.0	26.0	34.0
4	34.00939338758285	35.0	35.0	37.0	30.0	37.0
5	33.52131268893549	35.0	35.0	37.0	28.0	37.0
6	33.46961907408037	35.0	35.0	37.0	28.0	37.0
7	33.28024416944344	35.0	33.0	37.0	27.0	37.0
8	33.20596491438478	35.0	33.0	37.0	27.0	37.0
9	34.53805128184627	38.0	34.0	39.0	27.0	39.0
10-11	34.33981035991107	37.0	34.0	39.0	27.0	39.0
12-13	34.14979498188685	37.0	34.0	39.0	26.0	39.0
14-15	35.04216147191945	38.0	34.0	40.0	26.0	41.0
16-17	34.91379411437439	38.0	34.0	40.0	25.0	41.0
18-19	34.81379481398121	38.0	33.5	40.0	25.0	41.0
20-21	34.68511230022061	38.0	34.0	40.0	24.5	41.0
22-23	34.533399063059896	38.0	33.5	40.0	24.0	41.0
24-25	34.33668578259018	38.0	33.0	40.0	23.0	41.0
26-27	34.14275094063803	38.0	33.0	40.0	22.5	41.0
28-29	34.079155681173845	38.0	33.0	40.0	22.5	41.0
30-31	34.057544160181976	38.0	33.0	40.0	22.0	41.0
32-33	33.89941402931552	38.0	33.0	40.0	21.0	41.0
34-35	33.722674190438305	38.0	33.0	40.0	19.0	41.0
36-37	33.51017478177265	38.0	32.0	40.0	18.0	41.0
38-39	33.30853676900249	38.0	32.0	40.0	17.0	41.0
40-41	33.06415427862874	38.0	31.5	40.0	15.5	41.0
42-43	33.091998963249324	38.0	32.0	40.0	15.0	41.0
44-45	32.93163891892756	38.0	32.0	40.0	14.5	41.0
46-47	32.66723251532639	37.0	31.0	40.0	10.5	41.0
48-49	32.38455787847229	37.0	31.0	40.0	9.0	41.0
50-51	32.10127491683008	37.0	31.0	40.0	8.0	41.0
52-53	31.816676194645275	36.0	30.0	40.0	7.0	41.0
54-55	31.5044145190403	36.0	30.0	39.0	4.0	41.0
56-57	31.15775234318313	35.5	29.5	39.0	2.0	40.5
58-59	30.794033586457743	35.0	29.0	39.0	2.0	40.0
60-61	30.43023587410542	35.0	28.5	39.0	2.0	40.0
62-63	30.08037349676149	35.0	28.0	38.0	2.0	40.0
64-65	29.6569274734266	34.0	28.0	38.0	2.0	40.0
66-67	29.20094673460182	34.0	27.5	37.0	2.0	39.5
68-69	28.7442553951346	34.0	26.0	36.5	2.0	39.0
70-71	28.309225715148088	34.0	26.0	36.0	2.0	39.0
72-73	27.875110521220407	33.0	26.0	35.5	2.0	38.5
74-75	27.443481096956845	33.0	25.5	35.0	2.0	37.5
76-77	26.03097209368335	30.5	23.5	34.0	2.0	36.0
78-79	26.877726717584718	32.5	25.0	35.0	2.0	36.0
80-81	26.924821250457242	33.0	25.0	35.0	2.0	36.0
82-83	26.605895320163402	33.0	25.0	35.0	2.0	36.0
84-85	26.305006919444605	33.0	24.0	35.0	2.0	35.0
86-87	25.788698751301766	32.0	23.0	35.0	2.0	35.0
88-89	25.431932587219315	32.0	20.5	35.0	2.0	35.0
90-91	25.075802732197836	32.0	19.5	34.0	2.0	35.0
92-93	24.76800987844831	32.0	18.0	34.0	2.0	35.0
94-95	24.38631722305398	31.5	13.0	34.0	2.0	35.0
96-97	23.853952911796902	31.0	2.0	34.0	2.0	35.0
98-99	23.240768687997345	31.0	2.0	34.0	2.0	35.0
100-101	21.027121091280037	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	72201.0
3	32797.0
4	17743.0
5	12853.0
6	11440.0
7	11175.0
8	12715.0
9	14731.0
10	16764.0
11	18465.0
12	20293.0
13	22210.0
14	23630.0
15	25099.0
16	26436.0
17	27712.0
18	26950.0
19	25853.0
20	25899.0
21	27172.0
22	29097.0
23	33112.0
24	38886.0
25	45315.0
26	53485.0
27	63483.0
28	74423.0
29	87892.0
30	103499.0
31	122424.0
32	146792.0
33	179362.0
34	225547.0
35	293628.0
36	385571.0
37	423712.0
38	210519.0
39	12801.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.90148871919948	18.64762572969805	13.383152015183262	25.067733535919217
2	16.664634475424812	20.1104979001801	37.00516976126084	26.21969786313425
3	19.600562685376644	23.64438070956732	28.731405693890892	28.02365091116515
4	14.435812198732606	15.991015830251037	34.30827323950889	35.264898731507465
5	15.266607407249461	36.300713112012296	33.27665041252817	15.156029068210078
6	35.025509200187024	33.85023174719846	16.35410198210721	14.77015707050731
7	30.228472526182067	30.854933757513	20.129395704113332	18.787198012191606
8	27.64517778501957	33.41803336923467	19.573513415972016	19.36327542977374
9	27.228699659282622	15.190730672470618	18.755202253612094	38.82536741463466
10-11	26.491987502317965	24.624155692728852	26.785452233853935	22.098404571099245
12-13	27.115312523346528	21.903332684077398	26.630121562056413	24.351233230519654
14-15	24.030974657780394	23.51025554009287	24.043848802179266	28.41492099994747
16-17	24.48375697974831	26.4253854487874	24.878773231102592	24.2120843403617
18-19	24.586313677454026	25.523292527040642	26.132519652434137	23.757874143071188
20-21	25.210541926754402	24.838081843904032	25.814206539380592	24.137169689960974
22-23	24.945560827668803	24.58383332460884	25.93366806491957	24.536937782802788
24-25	24.756527746411855	24.871428416665765	25.74633352027887	24.625710316643517
26-27	24.396076082162654	25.101483220140828	25.806523661749935	24.69591703594658
28-29	24.493854769084127	25.26417702397686	25.700937927793643	24.541030279145374
30-31	24.725107836703017	25.01956464112442	25.494980838564597	24.760346683607974
32-33	24.48522289748147	25.10050435884076	25.317334171348705	25.096938572329066
34-35	24.904676967962864	25.14768697325757	25.43541375177393	24.512222307005636
36-37	25.135581297628395	24.770399008053072	25.34213138631648	24.751888308002055
38-39	24.819772691561653	24.798527822197606	25.434041012428832	24.947658473811906
40-41	24.96369071906955	24.756789881352752	25.617049508747968	24.662469890829733
42-43	24.963003500985824	24.682274916098788	25.369177830588267	24.985543752327118
44-45	24.527928727988876	24.745680250658346	25.65010967552207	25.076281345830704
46-47	24.87903603408153	24.757480536957036	25.39826713016053	24.965216298800907
48-49	24.647905038519173	24.88512549445222	25.232460221011504	25.234509246017105
50-51	24.44808112458604	25.068197470954225	25.490676930079225	24.993044474380508
52-53	24.900607731290883	24.61686541788731	25.52244867634119	24.960078174480614
54-55	24.320717792871548	24.65137324133133	25.650036658973917	25.3778723068232
56-57	24.369071497595325	24.88738729359196	25.4202955574431	25.323245651369618
58-59	24.273748190950354	24.655764171888368	25.49963752375419	25.57085011340709
60-61	24.03574466241754	24.71546638920429	25.757083246209817	25.491705702168353
62-63	24.09303046179552	24.93057782538547	25.580195126745757	25.39619658607325
64-65	23.903142906309185	24.811513411662638	25.438172284379657	25.847171397648523
66-67	23.941585794115188	24.801641591426502	25.409489675564394	25.847282938893912
68-69	24.162919178121705	24.568853339361993	25.47880407808114	25.789423404435162
70-71	23.83626370147732	24.48863645830036	25.509614151070707	26.165485689151613
72-73	23.867103721689052	24.4767421514239	25.432969435589097	26.223184691297952
74-75	23.803342706524315	24.68972526108591	25.342008661435305	26.16492337095447
76-77	23.794913893138286	24.312962777729258	25.663984439329766	26.228138889802686
78-79	23.665181928616878	24.46110243592285	25.58439993138386	26.28931570407641
80-81	23.72342869595046	24.482260140840637	25.513233318678054	26.281077844530852
82-83	23.63084215218966	24.461889279900202	25.554936571537855	26.35233199637228
84-85	23.334160749805918	24.409341254318946	25.595939772965803	26.66055822290933
86-87	23.36168956275202	24.392030104722533	25.574500904531206	26.67177942799424
88-89	23.175560092936223	24.312691305515262	25.513061655896625	26.998686945651883
90-91	22.988230075370808	24.38758713204789	25.5099821894545	27.114200603126797
92-93	23.12523161772265	24.184609500668547	25.566083325713734	27.12407555589507
94-95	22.82709419088706	24.04495587350243	25.751148241744676	27.376801693865833
96-97	22.924326582612483	23.81374860239156	25.80566546151396	27.456259353481993
98-99	22.673042396515715	23.942952254321128	25.692857554543576	27.691147794619585
100-101	22.80004597639878	23.676929176356136	25.79650779266643	27.726517054578647
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	41.0
1	35.0
2	47.0
3	68.5
4	59.5
5	69.5
6	145.5
7	292.0
8	425.0
9	460.0
10	411.0
11	381.5
12	397.5
13	431.0
14	458.5
15	517.5
16	597.5
17	654.5
18	735.0
19	832.5
20	1005.0
21	1152.5
22	1289.0
23	1538.0
24	1862.0
25	2344.0
26	2977.0
27	3847.5
28	4988.0
29	6510.5
30	8848.5
31	12579.5
32	17654.5
33	21294.5
34	24433.0
35	29366.5
36	35379.5
37	41560.5
38	48352.0
39	55204.5
40	61781.5
41	69091.5
42	77130.5
43	86017.5
44	93934.0
45	102210.0
46	111267.0
47	118931.5
48	126578.5
49	132632.0
50	135389.0
51	137782.0
52	140440.0
53	144265.5
54	145835.0
55	140907.5
56	132003.5
57	120575.0
58	110079.0
59	97761.5
60	82622.0
61	69058.0
62	56672.0
63	43967.0
64	32277.0
65	24280.5
66	18883.0
67	14537.5
68	10859.5
69	8265.5
70	6414.5
71	4978.5
72	3842.0
73	3218.0
74	2536.5
75	1838.5
76	1426.5
77	947.5
78	529.5
79	294.5
80	181.0
81	96.0
82	50.5
83	32.0
84	15.5
85	8.0
86	5.5
87	5.0
88	3.5
89	2.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.19172558355537522
2	0.0
3	0.012126518230088024
4	0.013559046482543479
5	0.00646303444131065
6	0.10527416925021471
7	0.14038776874063444
8	0.12176490145871355
9	0.1394882742565345
10-11	0.11227023745988088
12-13	0.11267001278614751
14-15	0.11421914217543074
16-17	0.06449708597101762
18-19	0.07702337952737227
20-21	0.03651281313235295
22-23	0.08218714415831635
24-25	0.0567514390246015
26-27	0.06426388369736208
28-29	0.03959441460565829
30-31	0.024536210649614913
32-33	0.031498964248758865
34-35	0.01915590105027641
36-37	0.02395320496547607
38-39	0.03203199801711438
40-41	0.024369637597003817
42-43	0.01294272618788241
44-45	0.02626857039677035
46-47	0.03328129591169762
48-49	0.00861182681999383
50-51	0.015824439998054426
52-53	0.00649634905183287
54-55	0.012476321640571332
56-57	0.004580758946805229
58-59	0.003664607157444183
60-61	0.022603963239326164
62-63	0.010110984293493724
64-65	0.007079354735971717
66-67	0.03941118424778608
68-69	0.08919986967324364
70-71	0.02135466534474292
72-73	0.04334230828940802
74-75	0.08426930731595511
76-77	0.24887679790624337
78-79	0.07967189106388876
80-81	0.016407445682193274
82-83	0.011726742903821386
84-85	0.012526293556354661
86-87	0.26218598480987015
88-89	0.6184857443450115
90-91	0.2649844120937367
92-93	0.3496868093464806
94-95	0.0010494102314499252
96-97	0.00433089936788858
98-99	0.0031648879996108853
100-101	0.004863933136244098
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3001686.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.86374402108563
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.98239865338097	35.89311900475928
2	13.11014780309471	13.074421082642255
3	5.583586899145234	8.352556435753957
4	2.918941933154567	5.821974934689287
5	1.7165292186856578	4.279628678262787
6	1.1055420025823521	3.3075878052794874
7	0.7451756702047685	2.601007420888205
8	0.5270738581206659	2.102550075322791
9	0.3720250489142896	1.6695505627644596
>10	1.817432589289206	15.531850251987454
>50	0.08556148063692794	2.9036867443483207
>100	0.032427300172438306	2.7984562918630576
>500	0.0019482709772436551	0.7103534994973835
>1k	0.0012092716408431815	0.9532572119412176
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.331461052221984E-5
2	0.0	0.0	0.0	0.0	3.331461052221984E-5
3	0.0	0.0	0.0	0.0	3.331461052221984E-5
4	0.0	0.0	0.0	0.0	3.331461052221984E-5
5	0.0	0.0	0.0	0.0	6.662922104443968E-5
6	0.0	0.0	0.0	0.0	1.3325844208887937E-4
7	0.0	0.0	0.0	0.0	1.3325844208887937E-4
8	0.0	0.0	0.0	0.0	1.6657305261109923E-4
9	0.0	0.0	0.0	0.0	1.6657305261109923E-4
10-11	0.0	0.0	0.0	0.0	1.6657305261109923E-4
12-13	0.0	0.0	0.0	0.0	3.8311802100552826E-4
14-15	0.0	0.0	0.0	0.0	4.497472420499679E-4
16-17	0.0	0.0	0.0	0.0	5.330337683555175E-4
18-19	0.0	0.0	0.0	1.665730526110992E-5	5.996629893999572E-4
20-21	0.0	0.0	0.0	6.662922104443968E-5	7.828933472721663E-4
22-23	0.0	0.0	0.0	1.8323035787220913E-4	8.328652630554961E-4
24-25	0.0	0.0	0.0	5.830056841388473E-4	8.328652630554961E-4
26-27	0.0	0.0	0.0	0.0015491293892832226	8.828371788388259E-4
28-29	0.0	0.0	0.0	0.006912781683360618	8.994944840999358E-4
30-31	0.0	0.0	0.0	0.027501210986092484	8.994944840999358E-4
32-33	0.0	0.0	0.0	0.07330880045414477	9.328090946221556E-4
34-35	0.0	0.0	0.0	0.13857212246717343	9.661237051443756E-4
36-37	0.0	0.0	0.0	0.2456119660750658	9.827810104054853E-4
38-39	0.0	0.0	0.0	0.4174154125381535	0.001116039452494365
40-41	0.0	0.0	0.0	0.6139049853982061	0.0011660113682776946
42-43	0.0	0.0	0.0	0.8235371721092746	0.0011660113682776946
44-45	0.0	0.0	0.0	1.0759786333413954	0.0012492978945832442
46-47	0.0	0.0	0.0	1.3820899321248126	0.0013492417261499038
48-49	0.0	0.0	0.0	1.7025764853485674	0.0014325282524554534
50-51	0.0	0.0	0.0	2.0410862428648433	0.0015324720840221128
52-53	0.0	0.0	0.0	2.391272771369157	0.0015991013050665527
54-55	0.0	0.0	0.0	2.7768061016375465	0.0015991013050665527
56-57	0.0	0.0	0.0	3.221606124024965	0.0016324159155887725
58-59	0.0	0.0	0.0	3.695073368766753	0.0016324159155887725
60-61	0.0	0.0	0.0	4.1993566282415955	0.0016324159155887725
62-63	0.0	0.0	0.0	4.72124665937743	0.0017490170524165419
64-65	0.0	0.0	0.0	5.26737306966818	0.001782331662938762
66-67	0.0	0.0	0.0	5.8991846582220795	0.0017989889681998717
68-69	0.0	0.0	0.0	6.596925860999452	0.0017989889681998717
70-71	0.0	0.0	0.0	7.317837375395028	0.0018489608839832015
72-73	0.0	0.0	0.0	8.067599342502847	0.001965562020810971
74-75	0.0	0.0	0.0	8.833835384513904	0.0020821631576387403
76-77	0.0	0.0	0.0	9.670848316579416	0.0020988204628998503
78-79	0.0	0.0	0.0	10.585367690024873	0.00213213507342207
80-81	0.0	0.0	0.0	11.518593217278557	0.00219876429446651
82-83	0.0	0.0	0.0	12.472357201919188	0.002365337347077609
84-85	0.0	0.0	0.0	13.442611918768318	0.002365337347077609
86-87	0.0	0.0	0.0	14.443216245803193	0.0024652811786442684
88-89	0.0	0.0	0.0	15.500288837673228	0.002565225010210928
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2645	0.0	46.39258	1
GTATCAA	4695	0.0	43.256042	1
TCAACGC	5950	0.0	33.90779	4
CAACGCA	6085	0.0	32.917637	5
ATCAACG	6245	0.0	32.382076	3
TATCAAC	6275	0.0	31.997639	2
AACGCAG	6610	0.0	30.806322	6
GTACATG	10220	0.0	26.619484	1
TACATGG	10630	0.0	25.095413	2
ACGCAGA	8050	0.0	24.824326	7
ACATGGG	10720	0.0	24.001148	3
CGCAGAG	8370	0.0	23.759846	8
TAAGGTG	1535	0.0	22.263884	5
CATGGGG	7765	0.0	20.84685	4
GCAGAGT	9485	0.0	20.16614	9
GTAAGGT	1540	0.0	19.111664	4
TAAGGTA	850	0.0	18.988363	4
AGAGTAC	8085	0.0	18.578735	10-11
CTATACT	650	0.0	18.258041	4
AGGTAAG	1460	0.0	18.2065	2
>>END_MODULE