FastQCFastQC Report
Mon 15 Aug 2016
SRR2920894_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920894_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2869713
Sequences flagged as poor quality0
Sequence length98
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25800.051.3787041
GTATCAA50650.043.2551571
ATCAACG65150.033.5097273
TCAACGC65150.033.5097274
CAACGCA66050.032.914245
TATCAAC68500.031.9384442
AACGCAG70600.031.2417566
ACGCAGA79000.027.451187
CGCAGAG81500.026.2706688
GTACATG79000.025.402111
TACATGG82250.024.0898322
ACATGGG84550.023.1628863
GCAGAGT92450.022.3652579
CATGGGG58950.020.4621054
GTGTAGC15450.019.6619151
AGAGTAC80000.019.59980610-11
TAAGGCG8550.019.3114455
GAGTACT44600.018.56053212-13
CAGAGTA85150.018.49514410-11
CGCCGTA3257.4760464E-1018.4055592
Produced by FastQC (version 0.11.5)