##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920894_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2869713
Sequences flagged as poor quality	0
Sequence length	98
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.66932686299989	31.0	30.0	33.0	22.0	34.0
2	28.699968951598994	31.0	28.0	34.0	20.0	34.0
3	28.766502782682448	31.0	28.0	34.0	22.0	34.0
4	32.2654742826199	35.0	32.0	37.0	23.0	37.0
5	32.23339720731655	35.0	32.0	37.0	23.0	37.0
6	32.354081749638375	35.0	33.0	37.0	25.0	37.0
7	32.19872196278861	35.0	32.0	37.0	23.0	37.0
8	32.14817196005315	35.0	32.0	37.0	23.0	37.0
9	33.42430654215247	37.0	33.0	39.0	23.0	39.0
10-11	33.23238874410089	37.0	33.0	39.0	21.5	39.0
12-13	33.062060212989934	37.0	32.0	39.0	19.0	39.0
14-15	33.81924011216452	38.0	32.5	40.0	18.0	41.0
16-17	33.72014518525023	38.0	32.0	40.0	18.0	41.0
18-19	33.618770413626734	38.0	32.0	40.0	17.5	41.0
20-21	33.53642994961517	38.0	32.0	40.0	18.0	41.0
22-23	33.31634696570703	38.0	32.0	40.0	17.0	41.0
24-25	33.12294103974857	38.0	32.0	40.0	16.0	41.0
26-27	33.14999775238848	38.0	32.0	40.0	15.5	41.0
28-29	32.98857603530388	38.0	32.0	40.0	11.5	41.0
30-31	32.73691428376287	38.0	31.5	40.0	9.5	41.0
32-33	32.47925803033265	37.0	31.0	40.0	9.0	41.0
34-35	32.142032670165975	37.0	30.5	40.0	8.5	41.0
36-37	31.820438141375114	37.0	30.0	40.0	7.0	41.0
38-39	31.6172300156845	37.0	30.0	40.0	2.0	41.0
40-41	31.41613778102549	37.0	30.0	40.0	2.0	41.0
42-43	31.17115439766973	36.5	30.0	40.0	2.0	41.0
44-45	30.884107226053615	36.0	29.0	39.0	2.0	41.0
46-47	30.6232449028875	36.0	28.5	39.0	2.0	40.0
48-49	30.31800880436476	35.5	28.0	39.0	2.0	40.0
50-51	29.180780273149267	34.0	26.0	38.0	2.0	39.5
52-53	29.631045682965507	34.5	27.5	38.5	2.0	39.5
54-55	30.28223501792688	36.0	28.0	39.0	2.0	40.0
56-57	30.242921853160926	35.5	28.0	39.0	2.0	41.0
58-59	30.006384436353045	35.0	28.0	39.0	2.0	40.5
60-61	29.68889467343947	35.0	28.0	39.0	2.0	40.0
62-63	29.307979752679103	35.0	27.0	39.0	2.0	40.0
64-65	28.900791298642062	34.0	26.0	38.0	2.0	40.0
66-67	28.51860917799097	34.0	26.0	38.0	2.0	40.0
68-69	28.075463295458466	34.0	26.0	37.0	2.0	40.0
70-71	27.749464319254226	34.0	25.0	37.0	2.0	39.0
72-73	27.289129609825096	33.5	24.5	36.0	2.0	39.0
74-75	26.841585726516904	33.0	23.0	36.0	2.0	39.0
76-77	26.42095202551614	33.0	22.0	35.0	2.0	37.5
78-79	25.942992731328882	32.0	20.5	35.0	2.0	37.0
80-81	25.475212155361877	32.0	19.0	35.0	2.0	36.5
82-83	24.98414249090414	32.0	17.5	35.0	2.0	36.0
84-85	24.56617299360598	31.5	10.5	35.0	2.0	35.5
86-87	24.08660778969883	31.0	4.5	34.0	2.0	35.0
88-89	23.525709191128172	31.0	2.0	34.0	2.0	35.0
90-91	23.008004807449385	31.0	2.0	34.0	2.0	35.0
92-93	22.45244803225967	30.0	2.0	34.0	2.0	35.0
94-95	21.784841376123673	30.0	2.0	34.0	2.0	35.0
96-97	21.12378067075	29.0	2.0	34.0	2.0	35.0
98	20.72052362030628	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	140633.0
3	23783.0
4	17548.0
5	14307.0
6	13342.0
7	13692.0
8	15780.0
9	18213.0
10	19833.0
11	21387.0
12	22722.0
13	22909.0
14	21968.0
15	23087.0
16	25286.0
17	26924.0
18	27276.0
19	26706.0
20	27735.0
21	29539.0
22	32050.0
23	35660.0
24	40495.0
25	46970.0
26	53928.0
27	62326.0
28	71809.0
29	83220.0
30	97575.0
31	114959.0
32	135522.0
33	163832.0
34	203969.0
35	255828.0
36	322462.0
37	355639.0
38	218067.0
39	22731.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.316791505807714	18.585873144029343	13.519673184048761	24.577662166114184
2	15.939941191807005	20.078647941364693	39.250843851560205	24.7305670152681
3	18.945266210319396	23.969628741513198	29.62354835561628	27.46155669255113
4	13.288324789238098	16.14344321287257	36.88661743399717	33.68161456389216
5	14.410967555743307	36.62038644231319	34.42488159819089	14.54376440375261
6	32.9296074695733	35.378243348090955	17.496007355806466	14.196141826529281
7	28.516954956279957	31.859258022660047	21.45730687047164	18.166480150588356
8	26.23172718167327	34.21708731990301	20.558327693413247	18.99285780501048
9	26.568092349304617	15.835276907481688	20.040366405978578	37.55626433723512
10-11	25.286679414749734	25.415263724153554	28.102222783641217	21.195834077455494
12-13	25.983849153758527	23.003630159351346	27.71637293518787	23.29614775170226
14-15	23.196786465631426	24.69615240800763	25.419908538189645	26.687152588171298
16-17	23.177216784540068	27.450158710466354	26.201523697144502	23.17110080784908
18-19	23.096400471098995	26.66915683772445	27.738106048867195	22.496336642309352
20-21	23.900676868695005	26.030638866540496	27.40751512919582	22.661169135568684
22-23	23.566830547818824	25.838122162673116	27.19538249302822	23.39966479647984
24-25	23.313573009636425	26.03803592076065	27.335689787675026	23.3127012819279
26-27	23.2002213727973	26.211892266633562	27.22986538037029	23.35802098019885
28-29	23.229492672368103	26.419536911516122	27.082391926494953	23.268578489620815
30-31	23.536860477055367	26.180954428000568	26.909013763311073	23.37317133163299
32-33	23.19075513594845	26.201919809392294	26.845632086838595	23.76169296782066
34-35	23.741469933745567	26.19660447766413	26.79813237180249	23.263793216787814
36-37	23.760088272383353	25.942868151884547	26.78415300546448	23.51289057026762
38-39	23.51211952832068	26.03239810468357	26.822603465643454	23.632878901352303
40-41	23.720037565313852	25.934230586877494	26.956474238976746	23.389257608831908
42-43	23.605578229554506	25.860882344140144	26.752690008509823	23.780849417795523
44-45	23.52313942999739	25.91596565736723	26.86228447506702	23.69861043756836
46-47	23.559782047733826	25.867328748167022	26.660262548997448	23.9126266551017
48-49	23.380339982321996	25.936233591739583	26.697300842145367	23.986125583793054
50-51	23.256829316417647	26.131729785629222	26.784854667556672	23.826586230396458
52-53	23.43078321165226	25.866680108994878	26.849177777591077	23.853358901761784
54-55	23.109672520775614	25.829234861968782	26.98819545730854	24.07289715994706
56-57	23.020338129517803	25.93276429568424	26.940388229001112	24.106509345796844
58-59	23.09179129362556	25.632022349050658	26.977419662979727	24.29876669434405
60-61	22.955625495510898	25.625605745940423	27.217574755545044	24.201194003003643
62-63	22.941237391867816	25.6363021837068	27.190039733023198	24.232420691402186
64-65	22.815198533663978	25.55043610614413	27.036323042003136	24.598042318188757
66-67	22.810942726017817	25.588027531006517	26.98248719167452	24.61854255130114
68-69	23.04603250726407	25.394207046465716	26.93060104862932	24.62915939764089
70-71	22.81566616456619	25.209763799412343	27.062673717409595	24.911896318611873
72-73	22.914695813321703	25.252073964311833	27.027179677825153	24.806050544541314
74-75	22.91728222258199	25.323553672338118	26.96855218801062	24.790611917069278
76-77	22.89591192258155	25.162429192096265	27.118520205941493	24.823138679380694
78-79	22.973480209590456	25.18688645307266	27.032927226041913	24.80670611129497
80-81	23.053282414984544	25.090750765873516	27.030648929175932	24.82531788996601
82-83	23.058955508203045	25.047440420374667	27.073447544699253	24.820156526723036
84-85	23.04809177349813	24.986016427790478	27.082179692268742	24.883712106442655
86-87	23.09439191115784	24.88504962433198	27.0692335085699	24.95132495594028
88-89	23.144821661343606	24.798913945540217	26.975255427255284	25.08100896586089
90-91	23.188104729385323	24.697168642875916	27.006683421997398	25.108043205741364
92-93	23.350433800281394	24.43861937144113	27.100816056126785	25.11013077215069
94-95	23.242984738483923	24.33402226978332	27.223586106564827	25.199406885167935
96-97	23.508307671755386	24.111718308351946	27.14286488648085	25.237109133411824
98	23.61478047738227	23.95709665247722	27.03692811360323	25.39119475653728
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	97.0
1	79.5
2	113.5
3	176.0
4	198.5
5	235.0
6	329.0
7	632.0
8	953.5
9	1075.5
10	1094.0
11	1098.0
12	1231.5
13	1403.5
14	1525.0
15	1680.5
16	1805.0
17	1883.5
18	1952.5
19	2023.0
20	2143.0
21	2247.0
22	2532.0
23	3002.0
24	3597.0
25	4496.5
26	5789.5
27	7719.0
28	10484.5
29	14362.5
30	19760.5
31	26984.0
32	33686.0
33	39507.5
34	46542.0
35	54460.0
36	62534.5
37	70629.0
38	77331.0
39	83024.0
40	89299.0
41	94674.0
42	100241.5
43	106313.5
44	111778.5
45	116914.0
46	120807.0
47	123790.0
48	125439.5
49	124039.0
50	122464.0
51	121592.5
52	119238.5
53	116209.5
54	111330.0
55	105092.0
56	96725.0
57	86329.5
58	77487.5
59	67168.0
60	55298.5
61	45391.5
62	37271.5
63	29189.0
64	21744.5
65	16845.0
66	13222.0
67	10270.0
68	8205.0
69	6526.0
70	4939.0
71	3798.5
72	3188.0
73	2617.5
74	2025.0
75	1539.0
76	1080.0
77	689.5
78	424.5
79	239.0
80	134.5
81	75.0
82	42.0
83	28.0
84	21.0
85	13.5
86	9.0
87	8.5
88	6.5
89	7.5
90	8.0
91	4.5
92	3.5
93	3.5
94	3.5
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.38683310839794777
2	0.025263850426854535
3	0.01927021970489732
4	0.0167264113170899
5	0.023312435773194046
6	0.023939676197584914
7	0.015123463565868783
8	0.010001000100010001
9	0.0
10-11	1.7423345121968644E-5
12-13	0.004948230014639095
14-15	0.003484669024393729
16-17	0.006254980898786743
18-19	0.03838362930369692
20-21	0.07819597290739527
22-23	0.09750103930253654
24-25	0.06429214350006429
26-27	0.07499007740495303
28-29	0.058019739256155584
30-31	0.05127690469395372
32-33	0.020002000200020003
34-35	0.3425081184076596
36-37	0.5196686916078368
38-39	0.47461192112242584
40-41	0.48391598741755704
42-43	0.43302239631628664
44-45	0.4073926556418708
46-47	0.406173021483333
48-49	0.39580613113576163
50-51	0.3938024464467353
52-53	0.4552720080370406
54-55	0.45504550455045506
56-57	0.425077350940669
58-59	0.410633397834557
60-61	0.424519803896766
62-63	0.4055109343686982
64-65	0.4554113947980164
66-67	0.47602321207730525
68-69	0.41755046584797856
70-71	0.37367848283086147
72-73	0.36010569698084793
74-75	0.3426126584783914
76-77	0.340608973789365
78-79	0.3399120399844862
80-81	0.3565861812662103
82-83	0.3510281341723022
84-85	0.3522129216405961
86-87	0.33712430476497124
88-89	0.3806826675698929
90-91	0.3597746534235305
92-93	0.35001758015522805
94-95	0.3565861812662103
96-97	0.35604605756742924
98	0.3511152508979121
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	2869713.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.33685848111394
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.7315858833895	49.86611313089909
2	11.883292962455878	15.053008893053702
3	4.223009888929204	8.02416539098361
4	1.8937754487730707	4.797831503757921
5	0.9959730792091541	3.1540902984434736
6	0.5871501569863121	2.2312947840123534
7	0.39202795234320637	1.7380873256741778
8	0.25704899958256106	1.3024540887418068
9	0.17772349484896763	1.013080305781624
>10	0.803058420561457	8.600747260696902
>50	0.037237096340009615	1.6055848821757055
>100	0.016734756292255945	1.827763429344104
>500	9.949394076792944E-4	0.41790333793789425
>1k	3.869208806232885E-4	0.3678753684977715
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	8.711672560984322E-5	0.0	0.0	0.0
12-13	0.0	1.0454007073181186E-4	0.0	0.0	0.0
14-15	0.0	1.219634158537805E-4	0.0	1.7423345121968644E-5	1.7423345121968644E-5
16-17	0.0	1.3938676097574915E-4	0.0	3.484669024393729E-5	3.484669024393729E-5
18-19	0.0	1.3938676097574915E-4	0.0	1.0454007073181187E-4	3.484669024393729E-5
20-21	0.0	1.3938676097574915E-4	0.0	2.43926831707561E-4	3.484669024393729E-5
22-23	0.0	2.0908014146362372E-4	0.0	5.052770085370907E-4	3.484669024393729E-5
24-25	0.0	2.0908014146362372E-4	0.0	0.0013415975743915854	5.2270035365905936E-5
26-27	0.0	2.0908014146362372E-4	0.0	0.0024566916621975785	6.969338048787458E-5
28-29	0.0	2.43926831707561E-4	0.0	0.008746519251228258	6.969338048787458E-5
30-31	0.0	2.43926831707561E-4	0.0	0.03625798119881675	6.969338048787458E-5
32-33	0.0	2.43926831707561E-4	0.0	0.09682152884277975	6.969338048787458E-5
34-35	0.0	2.43926831707561E-4	0.0	0.18083689902091254	6.969338048787458E-5
36-37	0.0	2.43926831707561E-4	0.0	0.31717457460031717	6.969338048787458E-5
38-39	0.0	2.43926831707561E-4	0.0	0.5348966952444374	6.969338048787458E-5
40-41	0.0	2.43926831707561E-4	0.0	0.7802173945617559	6.969338048787458E-5
42-43	0.0	2.7877352195149825E-4	0.0	1.039633580082747	6.969338048787458E-5
44-45	3.484669024393729E-5	3.1362021219543556E-4	0.0	1.3635161425550222	6.969338048787458E-5
46-47	3.484669024393729E-5	3.4846690243937287E-4	0.0	1.7415853083566195	6.969338048787458E-5
48-49	3.484669024393729E-5	4.1816028292724743E-4	0.0	2.142618443028972	6.969338048787458E-5
50-51	3.484669024393729E-5	4.704303182931533E-4	0.0	2.5656572625903706	6.969338048787458E-5
52-53	3.484669024393729E-5	5.227003536590593E-4	0.0	2.9932609985737253	6.969338048787458E-5
54-55	3.484669024393729E-5	5.227003536590593E-4	0.0	3.4807487717412857	6.969338048787458E-5
56-57	3.484669024393729E-5	5.227003536590593E-4	0.0	4.048087735602828	6.969338048787458E-5
58-59	3.484669024393729E-5	5.227003536590593E-4	0.0	4.639819382635127	6.969338048787458E-5
60-61	3.484669024393729E-5	5.227003536590593E-4	0.0	5.261153293029651	6.969338048787458E-5
62-63	3.484669024393729E-5	5.575470439029965E-4	0.0	5.886721076288813	6.969338048787458E-5
64-65	3.484669024393729E-5	6.272404243908711E-4	0.0	6.577591557065114	6.969338048787458E-5
66-67	3.484669024393729E-5	6.620871146348084E-4	0.0	7.359760366280531	6.969338048787458E-5
68-69	3.484669024393729E-5	6.969338048787457E-4	0.0	8.20531530504967	6.969338048787458E-5
70-71	3.484669024393729E-5	6.969338048787457E-4	0.0	9.082650425321278	6.969338048787458E-5
72-73	3.484669024393729E-5	6.969338048787457E-4	0.0	9.977809627652661	6.969338048787458E-5
74-75	3.484669024393729E-5	6.969338048787457E-4	0.0	10.912798596932864	6.969338048787458E-5
76-77	5.2270035365905936E-5	6.969338048787457E-4	0.0	11.926558509509487	6.969338048787458E-5
78-79	1.0454007073181186E-4	6.969338048787457E-4	0.0	13.015325922836187	6.969338048787458E-5
80-81	1.0454007073181186E-4	6.969338048787457E-4	0.0	14.122753738788512	6.969338048787458E-5
82-83	1.0454007073181186E-4	6.969338048787457E-4	0.0	15.24779655665915	6.969338048787458E-5
84-85	1.0454007073181186E-4	6.969338048787457E-4	0.0	16.3819692073737	6.969338048787458E-5
86	1.0454007073181186E-4	6.969338048787457E-4	0.0	17.269845451444098	6.969338048787458E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2580	0.0	51.378704	1
GTATCAA	5065	0.0	43.255157	1
ATCAACG	6515	0.0	33.509727	3
TCAACGC	6515	0.0	33.509727	4
CAACGCA	6605	0.0	32.91424	5
TATCAAC	6850	0.0	31.938444	2
AACGCAG	7060	0.0	31.241756	6
ACGCAGA	7900	0.0	27.45118	7
CGCAGAG	8150	0.0	26.270668	8
GTACATG	7900	0.0	25.40211	1
TACATGG	8225	0.0	24.089832	2
ACATGGG	8455	0.0	23.162886	3
GCAGAGT	9245	0.0	22.365257	9
CATGGGG	5895	0.0	20.462105	4
GTGTAGC	1545	0.0	19.661915	1
AGAGTAC	8000	0.0	19.599806	10-11
TAAGGCG	855	0.0	19.311445	5
GAGTACT	4460	0.0	18.560532	12-13
CAGAGTA	8515	0.0	18.495144	10-11
CGCCGTA	325	7.4760464E-10	18.40555	92
>>END_MODULE