FastQCFastQC Report
Mon 15 Aug 2016
SRR2920894_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920894_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2869713
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28000.051.13211
GTATCAA54050.046.904731
TCAACGC69950.034.9573174
ATCAACG70450.034.7766153
CAACGCA71100.034.1206135
TATCAAC73900.033.5998952
AACGCAG75550.032.1737026
ACGCAGA86700.027.2684577
CGCAGAG90050.025.7263938
GTACATG83600.024.2944341
TACATGG85750.022.8109722
ACATGGG87550.021.9100743
GCAGAGT100750.021.6273279
GTGGTAT15950.020.57661
AGAGTAC87950.019.4555710-11
CAGAGTA91100.019.14756410-11
GTATACG1507.654456E-419.0258981
CATGGGG62700.019.007494
TTAACGC2101.964255E-518.0879484
GAGTACA67250.017.6113321