##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920894_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2869713
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.87839202038671	31.0	30.0	34.0	26.0	34.0
2	29.898304813059703	31.0	30.0	34.0	26.0	34.0
3	30.032980998448277	31.0	30.0	34.0	26.0	34.0
4	33.66287952837096	35.0	35.0	37.0	28.0	37.0
5	33.131483531628426	35.0	33.0	37.0	27.0	37.0
6	33.06144482044023	35.0	33.0	37.0	26.0	37.0
7	32.86571165827384	35.0	33.0	37.0	26.0	37.0
8	32.7895232031914	35.0	33.0	37.0	26.0	37.0
9	34.07363105648544	37.0	34.0	39.0	26.0	39.0
10-11	33.86531858760789	37.0	33.0	39.0	25.0	39.0
12-13	33.67442667611709	37.0	33.0	39.0	24.5	39.0
14-15	34.510757695978654	38.0	33.0	40.0	24.0	41.0
16-17	34.382515951943624	38.0	33.0	40.0	23.5	41.0
18-19	34.29922661255672	38.0	33.0	40.0	22.5	41.0
20-21	34.181281717021875	38.0	33.0	40.0	21.5	41.0
22-23	34.02196317192695	38.0	33.0	40.0	19.0	41.0
24-25	33.82954183920134	38.0	32.5	40.0	18.5	41.0
26-27	33.6293308773386	38.0	32.0	40.0	18.0	41.0
28-29	33.5552482077476	38.0	32.0	40.0	18.0	41.0
30-31	33.508568801130984	38.0	32.0	40.0	17.0	41.0
32-33	33.33676590655581	38.0	32.0	40.0	16.0	41.0
34-35	33.14843139366201	38.0	32.0	40.0	16.0	41.0
36-37	32.92896484770428	38.0	31.5	40.0	15.0	41.0
38-39	32.726004656214755	37.5	31.0	40.0	11.0	41.0
40-41	32.47163357450728	37.0	31.0	40.0	9.0	41.0
42-43	32.47396934815433	37.0	31.0	40.0	9.0	41.0
44-45	32.3013587072993	37.0	31.0	40.0	8.0	41.0
46-47	32.03275170722647	37.0	30.0	40.0	6.5	41.0
48-49	31.75510913460684	37.0	30.0	40.0	2.0	41.0
50-51	31.48993226848817	36.5	30.0	40.0	2.0	41.0
52-53	31.226243704509823	36.0	30.0	39.5	2.0	41.0
54-55	30.939510501572805	36.0	29.0	39.0	2.0	41.0
56-57	30.615345680909556	35.5	28.5	39.0	2.0	40.0
58-59	30.28333164326886	35.0	28.0	39.0	2.0	40.0
60-61	29.959181283982055	35.0	28.0	39.0	2.0	40.0
62-63	29.644260070606364	34.5	28.0	38.0	2.0	40.0
64-65	29.251144103957433	34.0	27.0	38.0	2.0	40.0
66-67	28.83173979418848	34.0	26.0	37.5	2.0	40.0
68-69	28.39582494834849	34.0	26.0	37.0	2.0	39.5
70-71	27.972847110495024	33.5	26.0	36.5	2.0	39.0
72-73	27.532973332176425	33.0	25.0	36.0	2.0	39.0
74-75	27.088630640067493	33.0	24.0	35.5	2.0	38.5
76-77	25.672679463068256	30.5	22.0	34.0	2.0	36.0
78-79	26.504234918265347	32.5	24.0	35.0	2.0	37.0
80-81	26.522111096127034	33.0	24.0	35.0	2.0	36.5
82-83	26.168816707454717	33.0	23.0	35.0	2.0	36.0
84-85	25.833985140674343	32.0	22.0	35.0	2.0	36.0
86-87	25.27711359986173	32.0	20.0	35.0	2.0	35.0
88-89	24.88752220169752	32.0	18.0	34.5	2.0	35.0
90-91	24.496898296101385	31.5	15.0	34.0	2.0	35.0
92-93	24.164096200560824	31.0	8.5	34.0	2.0	35.0
94-95	23.748936391897033	31.0	2.0	34.0	2.0	35.0
96-97	23.184945846501027	31.0	2.0	34.0	2.0	35.0
98-99	22.55410035777097	31.0	2.0	34.0	2.0	35.0
100-101	20.422145524656997	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	86821.0
3	36766.0
4	19256.0
5	13491.0
6	11790.0
7	11836.0
8	13299.0
9	15179.0
10	17011.0
11	18921.0
12	20630.0
13	22864.0
14	24814.0
15	26445.0
16	28068.0
17	28666.0
18	27232.0
19	25544.0
20	25708.0
21	26808.0
22	28652.0
23	32664.0
24	37640.0
25	44444.0
26	52516.0
27	61584.0
28	72664.0
29	84143.0
30	99804.0
31	116867.0
32	138916.0
33	168586.0
34	209918.0
35	266993.0
36	343663.0
37	384949.0
38	210984.0
39	13577.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.18182443422611	20.156049310995755	14.318042725908942	24.34408352886919
2	16.052755101294103	21.370813039492102	37.975470020869686	24.600961838344112
3	18.715366696545697	25.00421698587972	30.01497204242915	26.265444275145434
4	13.821688749049866	17.280578643593227	36.43263882765704	32.46509377969986
5	14.329036673780527	37.029833602600576	34.36307725762811	14.27805246599078
6	32.758862472345655	36.09241906372552	17.198404542184957	13.950313921743868
7	28.355256844445144	32.12700805577802	21.461206813823765	18.056528285953075
8	26.11206806018766	34.6854285284643	20.391540066199866	18.810963345148174
9	26.410949515422317	15.820697183265702	20.028280408767767	37.74007289254422
10-11	25.275225765105212	25.692069845324454	28.09698650180129	20.935717887769044
12-13	25.953979833514683	23.170718093076463	27.801882405311957	23.073419668096896
14-15	23.080176990347727	24.88299665325947	25.427221388996752	26.609604967396056
16-17	23.023345664448833	27.691850898228022	26.278125526315698	23.006677911007447
18-19	23.052366259539188	26.829732715245875	27.813447325539524	22.30445369967541
20-21	23.85091509178108	26.104709004927013	27.455786192505666	22.588589710786238
22-23	23.48203503797539	25.99143624659634	27.265327261456047	23.261201453972223
24-25	23.25123872370055	26.18390466351449	27.374320667570178	23.190535945214783
26-27	23.12680645349135	26.365948205672346	27.24980063413513	23.25744470670117
28-29	23.247832946121317	26.490478663075343	27.16345889879099	23.098229492012347
30-31	23.4959322105972	26.234252804894243	26.96722519983423	23.30258978467433
32-33	23.200143194837874	26.245978963116695	26.880822192132403	23.67305564991302
34-35	23.681242482553248	26.279320874165567	26.85915977469356	23.180276868587622
36-37	23.69057853705481	26.04803124727147	26.84085477571958	23.420535439954143
38-39	23.498778749580396	26.10777887890362	26.873173658079864	23.520268713436124
40-41	23.697291531963618	25.96011105050079	26.984748855423472	23.35784856211212
42-43	23.56531988444701	25.997575046186693	26.750642048169382	23.686463021196914
44-45	23.43438448677996	25.99526065111759	26.89072466864729	23.679630193455154
46-47	23.48476252772751	25.967136049587864	26.706425915110206	23.841675507574422
48-49	23.344722243712923	26.024130689489095	26.690528315800403	23.940618750997576
50-51	23.215706871425155	26.158741267149637	26.712279950293915	23.913271911131297
52-53	23.472279868312516	25.9014483601967	26.7235890589405	23.902682712550284
54-55	23.127715996309956	25.87020791867685	26.827918446836087	24.174157638177103
56-57	23.055628059466603	26.004128831531613	26.688254052775022	24.25198905622675
58-59	23.0002695492464	25.74406133526019	26.737229211471753	24.518439904021648
60-61	22.86739054588598	25.764783751161957	26.93015772657358	24.43766797637848
62-63	22.872062362906785	25.77258365692037	26.852043529688107	24.503310450484737
64-65	22.666105128749763	25.61362160417815	26.761417187416413	24.958856079655675
66-67	22.58071094692466	25.769564426961384	26.678733138938092	24.97099148717586
68-69	22.847994346967102	25.59594800869928	26.605196444462738	24.95086119987088
70-71	22.596132732635184	25.443029989132505	26.65166431634105	25.309172961891264
72-73	22.56600987256722	25.54142850842797	26.60107615479909	25.29148546420571
74-75	22.506226303687374	25.685992011219028	26.39816745609327	25.409614229000326
76-77	22.528951054813998	25.445418309992	26.64999179065112	25.37563884454288
78-79	22.477800986847555	25.53988115073044	26.540451874136746	25.44186598828526
80-81	22.401440655976643	25.557981471454983	26.50992062904276	25.53065724352561
82-83	22.3403298774051	25.55385870552027	26.47982884041753	25.625982576657098
84-85	22.269391305650956	25.47484244234706	26.406744532248023	25.849021719753956
86-87	22.21282753912371	25.45645057769731	26.403958717588488	25.92676316559049
88-89	22.14098156534094	25.382488450176876	26.36052629779583	26.116003686686362
90-91	21.93412040283591	25.406523878855726	26.328516087825022	26.330839630483343
92-93	22.023579406914635	25.193512384635252	26.400651286390286	26.382256922059828
94-95	21.81459572948156	25.101692700392903	26.48996837620985	26.593743193915685
96-97	21.880396059201882	24.972635282416782	26.461028495200157	26.685940163181186
98-99	21.733257039547897	25.020115980957687	26.30149264237741	26.94513433711701
100-101	21.7403440737758	24.984483678502514	26.40076401983719	26.874408227884494
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	84.0
1	67.0
2	85.0
3	142.0
4	143.5
5	151.0
6	226.5
7	434.5
8	652.0
9	681.5
10	607.0
11	532.0
12	516.5
13	574.5
14	669.0
15	743.0
16	807.5
17	894.5
18	1057.0
19	1248.0
20	1467.5
21	1670.0
22	1958.0
23	2437.0
24	2957.0
25	3738.5
26	4922.0
27	6551.0
28	8649.0
29	11702.5
30	16193.5
31	22242.5
32	29240.0
33	35158.0
34	40858.0
35	47714.0
36	56073.0
37	64119.5
38	70966.0
39	77489.0
40	83528.5
41	89366.0
42	94818.5
43	100341.5
44	105983.0
45	111892.0
46	117224.5
47	120809.5
48	123473.5
49	124598.5
50	123231.0
51	120894.0
52	117522.0
53	114144.5
54	110837.5
55	104317.5
56	95190.5
57	85179.5
58	76526.0
59	66837.5
60	54974.5
61	45212.0
62	37365.0
63	29290.5
64	21971.0
65	17096.5
66	13478.5
67	10584.5
68	8357.0
69	6546.0
70	5111.5
71	3962.0
72	3118.5
73	2682.5
74	2152.0
75	1626.0
76	1197.0
77	814.0
78	492.5
79	251.0
80	141.5
81	79.0
82	50.5
83	29.5
84	13.0
85	8.0
86	4.5
87	1.0
88	2.5
89	3.0
90	1.5
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1868479530879917
2	0.0
3	0.01271904193903711
4	0.013311435673184043
5	0.006864797978055645
6	0.10715357250010715
7	0.14175633591233688
8	0.12349667022451373
9	0.1437425972562413
10-11	0.11476757431840745
12-13	0.11408806385865067
14-15	0.1164576387952384
16-17	0.06620871146348084
18-19	0.07894517674763991
20-21	0.03620571116345084
22-23	0.08347524647935177
24-25	0.05714857200005715
26-27	0.06516331075616272
28-29	0.03935933663052716
30-31	0.02474115007319547
32-33	0.0311703644232019
34-35	0.019305066395141256
36-37	0.02531612046222044
38-39	0.03247711530734955
40-41	0.02606532430246509
42-43	0.01324174229269617
44-45	0.026256981098806743
46-47	0.032982392315886636
48-49	0.008885906012204008
50-51	0.015559047193917997
52-53	0.006690564526835959
54-55	0.012893275390256795
56-57	0.004739149873175471
58-59	0.0037460192012232583
60-61	0.022092801614656238
62-63	0.010976707426840244
64-65	0.007404921676836674
66-67	0.040369890647601346
68-69	0.08988703748423622
70-71	0.02115194097806993
72-73	0.043889406362239015
74-75	0.08509561757569485
76-77	0.24821297460756528
78-79	0.08012996421593378
80-81	0.01618628761830887
82-83	0.011987261443914427
84-85	0.012771311974403015
86-87	0.263928831907581
88-89	0.6212990637042799
90-91	0.26845890163929287
92-93	0.35339770910888996
94-95	0.001324174229269617
96-97	0.004948230014639095
98-99	0.0036240557853694775
100-101	0.005366390297566343
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2869713.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.31077306308052
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.78609851642959	48.469119321196686
2	12.412926079835392	15.469180400188334
3	4.403880765892381	8.23227645001156
4	1.9828703825409764	4.942167456800577
5	1.0703585099162323	3.334743310376368
6	0.6196669748235996	2.316715694575137
7	0.38540702781232733	1.6810506892851202
8	0.25999727734102795	1.296050507633248
9	0.19376012480277766	1.0866008848734034
>10	0.8231697194682013	8.704152583671222
>50	0.04249557724494102	1.7941287031260882
>100	0.01790902057026714	1.8797406222342397
>500	0.0010669401203551934	0.43185219042270073
>1k	3.9308320195633215E-4	0.36222118560526884
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	3.484669024393729E-5	0.0	3.484669024393729E-5	0.0
12-13	0.0	3.484669024393729E-5	0.0	6.969338048787458E-5	0.0
14-15	0.0	5.2270035365905936E-5	0.0	6.969338048787458E-5	0.0
16-17	0.0	1.0454007073181186E-4	0.0	6.969338048787458E-5	0.0
18-19	0.0	1.0454007073181186E-4	0.0	1.219634158537805E-4	0.0
20-21	0.0	1.0454007073181186E-4	0.0	2.43926831707561E-4	3.484669024393729E-5
22-23	0.0	1.0454007073181186E-4	0.0	5.052770085370907E-4	3.484669024393729E-5
24-25	0.0	1.0454007073181186E-4	0.0	0.0013241742292696168	3.484669024393729E-5
26-27	0.0	1.0454007073181186E-4	0.0	0.0024566916621975785	3.484669024393729E-5
28-29	0.0	1.0454007073181186E-4	0.0	0.008607132490252509	3.484669024393729E-5
30-31	0.0	1.3938676097574915E-4	0.0	0.035857244261011466	3.484669024393729E-5
32-33	0.0	1.3938676097574915E-4	0.0	0.09596778493180327	3.484669024393729E-5
34-35	0.0	1.3938676097574915E-4	0.0	0.17959984151725278	3.484669024393729E-5
36-37	0.0	1.3938676097574915E-4	0.0	0.3159723637869013	3.484669024393729E-5
38-39	0.0	1.3938676097574915E-4	0.0	0.5347224617932176	3.484669024393729E-5
40-41	0.0	1.3938676097574915E-4	0.0	0.7796946942080967	3.484669024393729E-5
42-43	0.0	1.568101060977178E-4	0.0	1.0389366462778682	3.484669024393729E-5
44-45	0.0	1.7423345121968644E-4	0.0	1.3613904944501418	3.484669024393729E-5
46-47	0.0	2.0908014146362372E-4	0.0	1.7386581863761288	3.484669024393729E-5
48-49	0.0	2.613501768295297E-4	0.0	2.1337673837070117	3.484669024393729E-5
50-51	0.0	3.310435573174042E-4	0.0	2.5468226265135225	3.484669024393729E-5
52-53	0.0	3.833135926833101E-4	0.0	2.965941193422478	3.484669024393729E-5
54-55	0.0	3.833135926833101E-4	0.0	3.4441945936753955	3.484669024393729E-5
56-57	0.0	3.833135926833101E-4	0.0	4.0026999215601	3.484669024393729E-5
58-59	0.0	3.833135926833101E-4	0.0	4.586852413464342	3.484669024393729E-5
60-61	0.0	3.833135926833101E-4	0.0	5.200119315067395	3.484669024393729E-5
62-63	0.0	3.833135926833101E-4	0.0	5.818421563410697	3.484669024393729E-5
64-65	0.0	3.833135926833101E-4	0.0	6.496642695628448	3.484669024393729E-5
66-67	0.0	3.833135926833101E-4	0.0	7.263043377508483	3.484669024393729E-5
68-69	0.0	3.833135926833101E-4	0.0	8.092812765597117	3.484669024393729E-5
70-71	0.0	3.833135926833101E-4	0.0	8.953578284657734	3.484669024393729E-5
72-73	0.0	3.833135926833101E-4	0.0	9.836436605333006	3.484669024393729E-5
74-75	0.0	4.1816028292724743E-4	0.0	10.76309721564491	3.484669024393729E-5
76-77	0.0	4.1816028292724743E-4	0.0	11.76736140513006	3.484669024393729E-5
78-79	0.0	4.1816028292724743E-4	0.0	12.844943030888455	3.484669024393729E-5
80-81	0.0	4.1816028292724743E-4	0.0	13.942909970439551	3.484669024393729E-5
82-83	0.0	4.1816028292724743E-4	0.0	15.054571659256517	3.484669024393729E-5
84-85	0.0	4.3558362804921606E-4	0.0	16.179022083393008	3.484669024393729E-5
86-87	0.0	4.530069731711847E-4	0.0	17.3585128547698	3.484669024393729E-5
88-89	0.0	4.530069731711847E-4	0.0	18.597155882835672	3.484669024393729E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2800	0.0	51.1321	1
GTATCAA	5405	0.0	46.90473	1
TCAACGC	6995	0.0	34.957317	4
ATCAACG	7045	0.0	34.776615	3
CAACGCA	7110	0.0	34.120613	5
TATCAAC	7390	0.0	33.599895	2
AACGCAG	7555	0.0	32.173702	6
ACGCAGA	8670	0.0	27.268457	7
CGCAGAG	9005	0.0	25.726393	8
GTACATG	8360	0.0	24.294434	1
TACATGG	8575	0.0	22.810972	2
ACATGGG	8755	0.0	21.910074	3
GCAGAGT	10075	0.0	21.627327	9
GTGGTAT	1595	0.0	20.5766	1
AGAGTAC	8795	0.0	19.45557	10-11
CAGAGTA	9110	0.0	19.147564	10-11
GTATACG	150	7.654456E-4	19.025898	1
CATGGGG	6270	0.0	19.00749	4
TTAACGC	210	1.964255E-5	18.087948	4
GAGTACA	6725	0.0	17.611332	1
>>END_MODULE