FastQCFastQC Report
Mon 15 Aug 2016
SRR2920893_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920893_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3543826
Sequences flagged as poor quality0
Sequence length98
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45800.039.5914151
GGTATCA30200.035.8122181
CAACGCA57250.031.807525
TCAACGC56700.031.549784
AACGCAG61100.030.3227866
ATCAACG59500.030.2192633
TATCAAC60750.029.7493152
GTACATG98700.025.4126321
ACGCAGA74450.024.265377
TACATGG101850.023.8243852
ACATGGG101500.023.4087643
CGCAGAG78100.022.7768258
GTATAGG7550.020.7253681
CATGGGG70200.019.4059144
GAGTACA73150.018.3712541
GCAGAGT92850.018.0724839
GTGTAGC22850.016.7171481
GTATAGC12500.016.5681041
AGTACAT71950.016.4481092
GTACCGT3653.6598067E-916.3335086
Produced by FastQC (version 0.11.5)