##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920893_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3543826
Sequences flagged as poor quality	0
Sequence length	98
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.24474677932833	31.0	28.0	31.0	16.0	34.0
2	28.26944889506426	31.0	28.0	33.0	16.0	34.0
3	28.34903463093278	31.0	28.0	34.0	16.0	34.0
4	31.77659766591249	35.0	32.0	37.0	19.0	37.0
5	31.71262133073125	35.0	32.0	37.0	19.0	37.0
6	31.83475514881374	35.0	32.0	37.0	19.0	37.0
7	31.660202842916103	35.0	32.0	37.0	18.0	37.0
8	31.59156798330392	35.0	32.0	37.0	17.0	37.0
9	32.82433364392044	37.0	32.0	39.0	17.0	39.0
10-11	32.61836162384948	37.0	32.0	39.0	17.0	39.0
12-13	32.4351404386107	37.0	32.0	39.0	16.0	39.0
14-15	33.161732263378624	38.0	32.0	40.0	14.5	41.0
16-17	33.02738523279642	38.0	32.0	40.0	11.0	41.0
18-19	32.88815802468857	38.0	32.0	40.0	10.0	41.0
20-21	32.7871412140438	38.0	32.0	40.0	10.0	41.0
22-23	32.556423481288306	38.0	31.0	40.0	10.0	41.0
24-25	32.353182266849444	37.0	31.0	40.0	9.5	41.0
26-27	32.38607087368285	38.0	31.0	40.0	8.5	41.0
28-29	32.22009221107356	37.5	31.0	40.0	8.0	41.0
30-31	31.964086413949218	37.0	30.0	40.0	5.0	41.0
32-33	31.712123704719136	37.0	30.0	40.0	2.0	41.0
34-35	31.37593973293271	37.0	30.0	40.0	2.0	41.0
36-37	31.06548219353885	37.0	30.0	40.0	2.0	41.0
38-39	30.85514554044132	36.0	29.0	40.0	2.0	41.0
40-41	30.64731027990652	36.0	29.0	39.5	2.0	41.0
42-43	30.407763671241195	36.0	28.0	39.0	2.0	40.5
44-45	30.11873184518653	36.0	27.5	39.0	2.0	40.0
46-47	29.860344018018942	35.0	27.0	39.0	2.0	40.0
48-49	29.55020421431526	35.0	26.5	39.0	2.0	40.0
50-51	28.473202973283676	34.0	25.0	38.0	2.0	39.5
52-53	28.93518347119751	34.5	26.5	38.5	2.0	39.5
54-55	29.55426423306336	35.0	27.0	39.0	2.0	40.0
56-57	29.49797408224896	35.0	27.0	39.0	2.0	40.0
58-59	29.241978020365558	35.0	26.0	39.0	2.0	40.0
60-61	28.913625132836657	35.0	26.0	39.0	2.0	40.0
62-63	28.503773887318395	34.0	25.5	38.5	2.0	40.0
64-65	28.074387540471797	34.0	24.5	38.0	2.0	40.0
66-67	27.68023006772906	34.0	23.0	37.5	2.0	40.0
68-69	27.23106199345002	33.5	22.0	37.0	2.0	39.5
70-71	26.887543293604146	33.0	22.0	36.5	2.0	39.0
72-73	26.414794772655316	33.0	20.5	36.0	2.0	39.0
74-75	25.95766313018754	32.5	19.0	35.5	2.0	38.5
76-77	25.528751834881284	32.0	17.5	35.0	2.0	37.5
78-79	25.050439976454825	32.0	13.0	35.0	2.0	37.0
80-81	24.585538906255557	31.5	7.5	35.0	2.0	36.0
82-83	24.08925734502766	31.0	4.5	34.5	2.0	36.0
84-85	23.678966038400304	31.0	2.0	34.0	2.0	35.5
86-87	23.211050429676853	31.0	2.0	34.0	2.0	35.0
88-89	22.66566106800955	30.0	2.0	34.0	2.0	35.0
90-91	22.15112197946513	30.0	2.0	34.0	2.0	35.0
92-93	21.590725250054604	29.5	2.0	34.0	2.0	35.0
94-95	20.943531510858605	29.0	2.0	34.0	2.0	35.0
96-97	20.283606051764394	29.0	2.0	34.0	2.0	35.0
98	19.879341705828672	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	212337.0
3	35713.0
4	25816.0
5	20815.0
6	19080.0
7	19421.0
8	22361.0
9	25359.0
10	27673.0
11	29511.0
12	30409.0
13	29662.0
14	28716.0
15	30059.0
16	32133.0
17	34200.0
18	35211.0
19	35364.0
20	36571.0
21	38840.0
22	41753.0
23	46227.0
24	52194.0
25	59304.0
26	68086.0
27	77571.0
28	89307.0
29	103221.0
30	119263.0
31	139747.0
32	163876.0
33	197066.0
34	242339.0
35	304690.0
36	382269.0
37	417585.0
38	245897.0
39	24178.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.12513155494833	17.2604623710365	12.316856819502647	25.297549254512518
2	16.21583937928397	18.704291978137203	39.45890564647806	25.620962996100765
3	20.65537080338735	22.252747175211006	27.78621494502827	29.305667076373375
4	13.407441566952258	14.572264276719407	35.569863680210965	36.45043047611737
5	14.62317962774908	36.499539667872206	34.06762564018905	14.809655064189661
6	35.288911017066354	33.82215719247619	16.577589462471572	14.31134232798588
7	30.57245980679718	30.491518836978738	19.739267348757082	19.196754007467
8	27.172503553662775	34.73135663986756	19.12484369386772	18.971296112601934
9	26.723095321271416	15.89045286083459	18.853352280839974	38.53309953705402
10-11	26.227839628695087	25.181978460567763	26.81700512384073	21.77317678689642
12-13	26.73739960233183	22.064601042199897	26.988693604179637	24.20930575128864
14-15	23.37980002096666	23.866477656328243	24.39438313648519	28.359339186219913
16-17	23.980250245580883	26.94759539558064	25.20873316949046	23.86342118934801
18-19	23.939473193551496	26.24785938391188	26.60303263531806	23.20963478721856
20-21	24.54308123488192	25.495275669156065	26.535042170002487	23.426600925959526
22-23	24.003166829522634	25.402395938707784	26.34336402764677	24.25107320412281
24-25	23.84755814427916	25.586108762439117	26.474125603417324	24.092207489864396
26-27	23.699113055053616	25.87489414069637	26.334533189768493	24.091459614481526
28-29	23.800574387144387	26.018221489148928	26.21378246809164	23.96742165561504
30-31	23.996262628943256	25.770624568401118	26.12893280806491	24.104179994590723
32-33	23.735043470775587	25.77834631835481	26.187187516316534	24.299422694553062
34-35	24.067257583647695	25.89591921344563	26.14315768554669	23.893665517359985
36-37	24.262332274123956	25.541168079654813	26.129163990284198	24.06733565593703
38-39	23.992126479757488	25.736569292704125	26.263714739096102	24.00758948844229
40-41	24.18190333203306	25.656450427730356	26.2226516787169	23.938994561519692
42-43	24.0107208098354	25.55585370214476	26.149697481958434	24.283728006061413
44-45	23.930500010977113	25.761878828267694	26.281499884209175	24.02612127654602
46-47	24.201479856532583	25.670841053165177	26.028829738881427	24.098849351420814
48-49	23.96211042185749	25.771444846075003	26.061816990629715	24.20462774143779
50-51	24.00507850366832	25.92020971132828	26.119327841770478	23.95538394323292
52-53	23.932991676035652	25.760658888639803	26.218886451898506	24.087462983426043
54-55	23.593947501176153	25.66468090895178	26.517262488451053	24.224109101421014
56-57	23.808186106576716	25.78659412390606	26.39509313559453	24.01012663392269
58-59	23.776904059691837	25.51129646852391	26.432331245607294	24.279468226176963
60-61	23.708575009803052	25.536278103035208	26.569324597848087	24.18582228931365
62-63	23.62360736168513	25.580878541260056	26.620757640566882	24.17475645648793
64-65	23.443430342494636	25.39476100648894	26.538247237324796	24.623561413691622
66-67	23.434857566922034	25.56140408564977	26.494361014150048	24.509377333278152
68-69	23.679807013331576	25.318791340247365	26.53731007124387	24.46409157517719
70-71	23.439700023674593	25.199931355013522	26.57465765295315	24.78571096835874
72-73	23.38444349022584	25.248567574887403	26.658602528452775	24.708386406433984
74-75	23.386470064159855	25.36436190767922	26.614124454210902	24.635043573950018
76-77	23.37323427504387	25.027623402223632	26.75270239397157	24.846439928760923
78-79	23.29705259456777	25.13061377362053	26.7475634568494	24.8247701749623
80-81	23.373811093216634	25.078330807720445	26.698998475571774	24.848859623491144
82-83	23.272249248434722	25.12178783350444	26.785027698485997	24.820935219574842
84-85	23.317163440120126	24.999536378437508	26.787600876619898	24.89569930482247
86-87	23.183576629059406	24.995134368569847	26.833302688701927	24.987986313668824
88-89	23.08365365485141	24.855354390416764	26.86296847243276	25.198023482299064
90-91	23.09468838518891	24.8345146234199	26.884033103304557	25.186763888086638
92-93	23.135953876136394	24.656287155251995	27.021780099939	25.18597886867261
94-95	23.06000209807941	24.559817418957437	27.10244332814288	25.27773715482028
96-97	23.182560977698788	24.257408431697854	27.130501456267314	25.429529134336043
98	23.084367176807476	24.257509840128698	27.082876788734662	25.575246194329164
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	39.0
1	35.5
2	62.0
3	109.5
4	122.0
5	130.5
6	210.5
7	380.0
8	581.0
9	744.0
10	799.5
11	888.0
12	1073.0
13	1240.5
14	1387.5
15	1567.0
16	1726.5
17	1795.0
18	1853.0
19	1876.0
20	1926.5
21	2060.0
22	2245.0
23	2617.5
24	3212.0
25	4142.5
26	5667.5
27	7718.0
28	10484.0
29	14565.0
30	20046.0
31	27894.0
32	34695.5
33	39643.5
34	46654.0
35	54840.0
36	63045.5
37	71872.0
38	80164.5
39	89084.5
40	99234.0
41	108390.5
42	118040.5
43	127392.0
44	135473.5
45	143033.0
46	150448.5
47	158316.5
48	163686.5
49	165134.5
50	165223.0
51	163962.0
52	160100.5
53	156730.5
54	151230.5
55	142124.0
56	130983.0
57	118103.5
58	107330.5
59	94547.0
60	78612.0
61	64738.0
62	52913.0
63	40512.5
64	29404.0
65	21536.0
66	15809.0
67	11883.0
68	9046.0
69	6860.0
70	5110.5
71	3708.5
72	2820.0
73	2271.0
74	1742.0
75	1294.0
76	860.5
77	474.5
78	274.0
79	162.5
80	98.5
81	68.0
82	47.5
83	31.0
84	24.0
85	16.5
86	10.0
87	10.0
88	9.0
89	8.0
90	8.5
91	6.0
92	4.0
93	4.5
94	5.0
95	3.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.36632724067152284
2	0.023167051655470672
3	0.01808779550689001
4	0.015237768445741977
5	0.02065564167089468
6	0.02347745064232838
7	0.014193699126311505
8	0.00843720882458676
9	0.0
10-11	0.0
12-13	0.004740639072008615
14-15	0.0029487903751482156
16-17	0.005276782776581017
18-19	0.037515950275211034
20-21	0.07738811104156919
22-23	0.09527837992045884
24-25	0.06282757674897131
26-27	0.07249227247613173
28-29	0.05542032819895785
30-31	0.04977671025609045
32-33	0.01808779550689001
34-35	0.3218696403265849
36-37	0.49694877795918874
38-39	0.45295677609453733
40-41	0.4619301286236966
42-43	0.41240738117503506
44-45	0.3880974968861338
46-47	0.3862068848752732
48-49	0.37689491526954205
50-51	0.374129542477537
52-53	0.43283727812821504
54-55	0.4335991665505022
56-57	0.4041253718438772
58-59	0.3902279626595662
60-61	0.40391373617101967
62-63	0.3853462331389859
64-65	0.43348629419164486
66-67	0.4532953931711094
68-69	0.3964218333518632
70-71	0.3559429836566468
72-73	0.34258171817690825
74-75	0.32606002664916395
76-77	0.3243669412663037
78-79	0.3235203985748736
80-81	0.33889925746918725
82-83	0.33477941637089403
84-85	0.3343984721597505
86-87	0.3215874594294415
88-89	0.36312448748894555
90-91	0.34126957700519156
92-93	0.3321692430723179
94-95	0.3393084197700451
96-97	0.3392519835906165
98	0.3346665440120367
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3543826.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.33291698789143
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.26157514310037	36.3713586309195
2	12.221274716303752	12.302648115638663
3	5.447603997203061	8.225813993223817
4	3.0734279234205775	6.187783701511818
5	1.819784388941753	4.5797528292233
6	1.2400063747547612	3.7447882754992547
7	0.8352724720769773	2.942918999952495
8	0.5993977324291486	2.4135549047269356
9	0.4388098185073638	1.9877920351562564
>10	1.9727941726801552	16.335278214554723
>50	0.06587691531474997	2.238360736397871
>100	0.022649538752785223	1.9556273608370935
>500	9.613226263887339E-4	0.3308760275062544
>1k	5.654838880754458E-4	0.38344617485193344
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.821808971433699E-5
2	0.0	0.0	0.0	2.821808971433699E-5	2.821808971433699E-5
3	0.0	0.0	0.0	2.821808971433699E-5	2.821808971433699E-5
4	0.0	0.0	0.0	5.643617942867398E-5	2.821808971433699E-5
5	0.0	0.0	0.0	5.643617942867398E-5	2.821808971433699E-5
6	0.0	0.0	0.0	5.643617942867398E-5	2.821808971433699E-5
7	0.0	0.0	0.0	5.643617942867398E-5	2.821808971433699E-5
8	0.0	0.0	0.0	5.643617942867398E-5	2.821808971433699E-5
9	0.0	0.0	0.0	5.643617942867398E-5	1.1287235885734796E-4
10-11	0.0	0.0	0.0	8.465426914301097E-5	1.1287235885734796E-4
12-13	0.0	0.0	0.0	1.1287235885734796E-4	1.1287235885734796E-4
14-15	0.0	0.0	0.0	1.1287235885734796E-4	1.5519949342885346E-4
16-17	0.0	0.0	0.0	1.2698140371451646E-4	1.6930853828602194E-4
18-19	0.0	0.0	0.0	1.8341758314319043E-4	1.6930853828602194E-4
20-21	0.0	0.0	0.0	2.2574471771469591E-4	1.6930853828602194E-4
22-23	0.0	0.0	0.0	3.103989868577069E-4	1.9752662800035892E-4
24-25	0.0	0.0	0.0	6.349070185725824E-4	2.2574471771469591E-4
26-27	0.0	0.0	0.0	0.0010017421848589631	2.2574471771469591E-4
28-29	0.0	0.0	0.0	0.0038376602011498304	2.2574471771469591E-4
30-31	0.0	0.0	0.0	0.016691000066030328	2.2574471771469591E-4
32-33	0.0	0.0	0.0	0.042030844629504946	2.2574471771469591E-4
34-35	0.0	0.0	0.0	0.07538462667185128	2.539628074290329E-4
36-37	1.4109044857168495E-5	1.4109044857168495E-5	0.0	0.1361805009613903	2.539628074290329E-4
38-39	2.821808971433699E-5	2.821808971433699E-5	0.0	0.24192779216586818	2.9628994200053837E-4
40-41	2.821808971433699E-5	2.821808971433699E-5	0.0	0.36426732012237617	3.5272612142921237E-4
42-43	2.821808971433699E-5	2.821808971433699E-5	0.0	0.49418340516718373	3.809442111435494E-4
44-45	2.821808971433699E-5	2.821808971433699E-5	0.0	0.6497215156726093	3.950532560007179E-4
46-47	2.821808971433699E-5	2.821808971433699E-5	0.0	0.8374000303626645	4.0916230085788637E-4
48-49	4.2327134571505486E-5	2.821808971433699E-5	0.0	1.0451133887498991	4.2327134571505486E-4
50-51	5.643617942867398E-5	2.821808971433699E-5	0.0	1.2810307278066135	4.2327134571505486E-4
52-53	5.643617942867398E-5	2.821808971433699E-5	0.0	1.522563466716481	4.3738039057222334E-4
54-55	5.643617942867398E-5	2.821808971433699E-5	0.0	1.7921449868023993	4.5148943542939183E-4
56-57	5.643617942867398E-5	2.821808971433699E-5	0.0	2.1034046253963936	4.5148943542939183E-4
58-59	5.643617942867398E-5	2.821808971433699E-5	0.0	2.448652953051307	4.5148943542939183E-4
60-61	5.643617942867398E-5	2.821808971433699E-5	0.0	2.8278899697671385	4.5148943542939183E-4
62-63	5.643617942867398E-5	2.821808971433699E-5	0.0	3.2142661631806977	4.7970752514372886E-4
64-65	5.643617942867398E-5	2.821808971433699E-5	0.0	3.6152452180214265	4.938165700008974E-4
66-67	5.643617942867398E-5	2.821808971433699E-5	0.0	4.072378271393686	5.079256148580658E-4
68-69	5.643617942867398E-5	2.821808971433699E-5	0.0	4.586413102674905	5.220346597152343E-4
70-71	5.643617942867398E-5	2.821808971433699E-5	0.0	5.142944377065917	5.361437045724028E-4
72-73	5.643617942867398E-5	2.821808971433699E-5	0.0	5.711341358181807	5.925798840010769E-4
74-75	5.643617942867398E-5	2.821808971433699E-5	0.0	6.284521305504277	5.925798840010769E-4
76-77	5.643617942867398E-5	2.821808971433699E-5	0.0	6.899732097456251	5.925798840010769E-4
78-79	5.643617942867398E-5	2.821808971433699E-5	0.0	7.578320718906628	5.925798840010769E-4
80-81	5.643617942867398E-5	2.821808971433699E-5	0.0	8.300816687952512	5.925798840010769E-4
82-83	7.054522428584247E-5	2.821808971433699E-5	0.0	9.040201183692428	5.925798840010769E-4
84-85	8.465426914301097E-5	2.821808971433699E-5	0.0	9.770753417351756	5.925798840010769E-4
86	8.465426914301097E-5	2.821808971433699E-5	0.0	10.32751043646048	5.925798840010769E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4580	0.0	39.591415	1
GGTATCA	3020	0.0	35.812218	1
CAACGCA	5725	0.0	31.80752	5
TCAACGC	5670	0.0	31.54978	4
AACGCAG	6110	0.0	30.322786	6
ATCAACG	5950	0.0	30.219263	3
TATCAAC	6075	0.0	29.749315	2
GTACATG	9870	0.0	25.412632	1
ACGCAGA	7445	0.0	24.26537	7
TACATGG	10185	0.0	23.824385	2
ACATGGG	10150	0.0	23.408764	3
CGCAGAG	7810	0.0	22.776825	8
GTATAGG	755	0.0	20.725368	1
CATGGGG	7020	0.0	19.405914	4
GAGTACA	7315	0.0	18.371254	1
GCAGAGT	9285	0.0	18.072483	9
GTGTAGC	2285	0.0	16.717148	1
GTATAGC	1250	0.0	16.568104	1
AGTACAT	7195	0.0	16.448109	2
GTACCGT	365	3.6598067E-9	16.333508	6
>>END_MODULE