FastQCFastQC Report
Mon 15 Aug 2016
SRR2920893_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920893_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3543826
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA47100.041.4983331
GGTATCA29650.041.2209631
ATCAACG59600.032.4191973
TCAACGC59500.032.3141064
CAACGCA60500.031.6956965
TATCAAC61950.031.1110212
AACGCAG63250.030.2430086
GTACATG100600.026.425521
TACATGG104500.024.439682
ACATGGG102850.023.5407313
ACGCAGA81550.023.0486537
CGCAGAG83050.022.4602688
CATGGGG71850.019.5577564
GAGTACA78500.018.962011
GTATAGG9800.018.4402681
GCAGAGT99250.018.0763659
GTGTAGC22200.017.3516881
AGTACAT75400.016.9359862
GTATAAT10800.016.7328361
AGAGTAC83750.016.60335210-11
Produced by FastQC (version 0.11.5)