##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920893_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3543826
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.378233581445592	31.0	30.0	34.0	27.0	34.0
2	30.379302200503073	31.0	30.0	34.0	27.0	34.0
3	30.523491277506288	31.0	30.0	34.0	27.0	34.0
4	34.168298895036045	35.0	35.0	37.0	30.0	37.0
5	33.72197986018501	35.0	35.0	37.0	28.0	37.0
6	33.67837529269214	35.0	35.0	37.0	29.0	37.0
7	33.49795249541033	35.0	35.0	37.0	28.0	37.0
8	33.431964774794245	35.0	35.0	37.0	28.0	37.0
9	34.78429556078656	38.0	35.0	39.0	27.0	39.0
10-11	34.59628477809012	38.0	34.5	39.0	27.0	39.0
12-13	34.41778123418023	37.0	34.0	39.0	27.0	39.0
14-15	35.341034097046524	38.5	34.0	40.0	27.0	41.0
16-17	35.220552730297705	38.0	34.0	40.0	26.5	41.0
18-19	35.12192514530905	38.5	34.0	40.0	26.0	41.0
20-21	35.00269412211547	38.0	34.0	40.0	25.0	41.0
22-23	34.8642551863438	38.0	34.0	40.0	25.0	41.0
24-25	34.69310598206572	38.0	34.0	40.0	24.5	41.0
26-27	34.51104992175124	38.0	33.0	40.0	24.0	41.0
28-29	34.458647236066334	38.0	33.5	40.0	24.0	41.0
30-31	34.4517947551601	38.0	33.0	40.0	24.0	41.0
32-33	34.30988781616253	38.0	33.0	40.0	24.0	41.0
34-35	34.14972490184338	38.0	33.0	40.0	23.0	41.0
36-37	33.96325129958412	38.0	33.0	40.0	22.0	41.0
38-39	33.78131403742734	38.0	33.0	40.0	20.5	41.0
40-41	33.54691243305964	38.0	32.5	40.0	19.0	41.0
42-43	33.58055291089348	38.0	33.0	40.0	19.0	41.0
44-45	33.4431768094709	38.0	32.0	40.0	18.0	41.0
46-47	33.20198635598926	38.0	32.0	40.0	15.5	41.0
48-49	32.93848625750813	37.5	32.0	40.0	14.5	41.0
50-51	32.67152055433873	37.0	31.0	40.0	12.5	41.0
52-53	32.40361829277171	37.0	31.0	40.0	9.0	41.0
54-55	32.12702937446703	37.0	31.0	40.0	8.0	41.0
56-57	31.805756264557008	36.0	30.0	39.5	7.5	41.0
58-59	31.470211291412163	36.0	30.0	39.0	6.5	40.5
60-61	31.137057095918365	35.0	29.0	39.0	2.0	40.0
62-63	30.8075104421041	35.0	29.0	39.0	2.0	40.0
64-65	30.395314978782817	35.0	28.5	38.0	2.0	40.0
66-67	29.954179889193206	34.5	28.0	38.0	2.0	40.0
68-69	29.503453753090586	34.0	28.0	37.0	2.0	40.0
70-71	29.05726141746237	34.0	27.5	37.0	2.0	39.0
72-73	28.600680309924922	34.0	26.0	36.0	2.0	39.0
74-75	28.136278699913596	33.5	26.0	36.0	2.0	38.5
76-77	26.710869410631332	31.5	25.0	34.5	2.0	36.0
78-79	27.536880337804398	33.0	26.0	35.0	2.0	37.0
80-81	27.553755037634467	33.0	26.0	35.0	2.0	37.0
82-83	27.19808054910145	33.0	26.0	35.0	2.0	36.0
84-85	26.86073173457162	33.0	25.0	35.0	2.0	36.0
86-87	26.306883012879304	32.5	24.0	35.0	2.0	35.0
88-89	25.91811844035232	32.0	23.5	35.0	2.0	35.0
90-91	25.53740166701187	32.0	21.0	34.5	2.0	35.0
92-93	25.205925460222936	32.0	20.0	34.0	2.0	35.0
94-95	24.80952450825746	32.0	18.5	34.0	2.0	35.0
96-97	24.25469915848013	31.5	6.5	34.0	2.0	35.0
98-99	23.62842123174219	31.0	2.0	34.0	2.0	35.0
100-101	21.40869684346805	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	74520.0
3	34638.0
4	18555.0
5	13407.0
6	11256.0
7	11372.0
8	12905.0
9	14964.0
10	17430.0
11	19282.0
12	22158.0
13	24033.0
14	26364.0
15	28242.0
16	29726.0
17	31194.0
18	30344.0
19	29765.0
20	29756.0
21	30995.0
22	33191.0
23	37630.0
24	43976.0
25	50949.0
26	61002.0
27	72797.0
28	85737.0
29	101124.0
30	119284.0
31	141150.0
32	169720.0
33	207431.0
34	260034.0
35	336414.0
36	448867.0
37	532832.0
38	309193.0
39	21587.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.30877682107991	18.569026001029602	12.989348039158868	25.132849138731622
2	16.458116171617906	19.73468787688786	38.31277269256448	25.494423258929753
3	20.486397245583337	23.051842862787154	28.16108822035333	28.300671671276174
4	13.959268592315935	15.483218042540988	35.17365866681869	35.383854698324384
5	14.618268058820858	36.746046998326264	34.086894781058554	14.548790161794326
6	35.34240987489152	34.360508343578246	16.25277403637547	14.044307745154757
7	30.534949768979697	30.547807779803033	19.802834414254022	19.114408036963248
8	27.132043144913386	35.214114091714585	18.911640853857005	18.74220190951503
9	26.57906018108895	15.846314313464566	18.810027289659516	38.764598215786975
10-11	26.234861791787527	25.38303277629106	26.83152631842873	21.550579113492685
12-13	26.745363234978157	22.129934938877803	27.1140132636602	24.010688562483836
14-15	23.318774714137778	23.951304081522238	24.45500527814101	28.27491592619897
16-17	23.922793899639412	27.120626413623878	25.245508170428334	23.711071516308387
18-19	23.939331552455823	26.345097687033793	26.627310007961135	23.088260752549246
20-21	24.469035579422147	25.540237613935368	26.59695822320534	23.393768583437144
22-23	23.95544363399692	25.499400060467014	26.40900356941781	24.13615273611825
24-25	23.81359769027902	25.68724603664343	26.55697722565534	23.94217904742221
26-27	23.67860359371424	25.975088901146954	26.38356755816536	23.96273994697345
28-29	23.85860284762467	26.036580942081212	26.269065159661935	23.835751050632187
30-31	23.985540748568553	25.86451239965204	26.221138935601747	23.928807916177664
32-33	23.759954847786997	25.86234418061901	26.180633974217564	24.197066997376425
34-35	24.0028415850219	25.940371872824112	26.235537706743795	23.82124883541019
36-37	24.185061522857428	25.679493495747824	26.21221317875788	23.92323180263687
38-39	23.960691058075238	25.818210610151233	26.351370137844377	23.869728193929156
40-41	24.175800447769788	25.706541435640712	26.22634277307917	23.891315343510332
42-43	24.000576854177673	25.665015642654076	26.153210945610468	24.181196557557787
44-45	23.880452305552797	25.859624279239107	26.333477331446353	23.92644608376174
46-47	24.108072235778675	25.775579388377466	26.080864203019622	24.03548417282423
48-49	23.824422880546507	25.883445737867554	26.153487536635478	24.13864384495046
50-51	23.94260740650011	26.003142004462248	26.13952695518375	23.91472363385389
52-53	23.9878473388257	25.798894114605943	26.13399251492697	24.079266031641396
54-55	23.577662539245775	25.74112251737397	26.42723392246093	24.253981020919323
56-57	23.733805805401296	25.875817769965327	26.274363781104746	24.116012643528627
58-59	23.733740667052235	25.628245993483358	26.311727404343856	24.326285935120552
60-61	23.725770064377954	25.605951256328353	26.417992973717347	24.250285705576346
62-63	23.61257801146588	25.71299117997206	26.39572599679495	24.27870481176711
64-65	23.380256252332565	25.58510431901094	26.313901699624182	24.72073772903231
66-67	23.390404674862733	25.709910087935974	26.259883975327114	24.63980126187418
68-69	23.637925285806947	25.54045697195531	26.29202194972498	24.529595792512765
70-71	23.46253027226374	25.369896333769727	26.252087359109876	24.915486034856656
72-73	23.456882309698006	25.436154019014108	26.272495830289095	24.83446784099879
74-75	23.406374431666297	25.599231343537554	26.127146047975785	24.867248176820357
76-77	23.39276266410739	25.302036516347414	26.378400257624683	24.92680056192051
78-79	23.348725940843305	25.423204924165887	26.266967204468926	24.961101930521885
80-81	23.398323698728625	25.39787636519632	26.22922527335677	24.974574662718275
82-83	23.321804922927566	25.522461350898283	26.228022644789494	24.92771108138465
84-85	23.327536101000252	25.382790192635092	26.22564897840537	25.06402472795929
86-87	23.186914366214477	25.41756543828298	26.214765075987668	25.180755119514874
88-89	23.149166321066307	25.41547646919434	26.12633653633049	25.309020673408867
90-91	23.13148591403738	25.407006955232735	26.09370231920788	25.367804811522003
92-93	23.09002715935722	25.32267046670172	26.201537128148246	25.38576524579282
94-95	23.02421546884076	25.198668035634746	26.250296822172093	25.526819673352403
96-97	23.08308031199827	25.020420193903302	26.278352111163738	25.618147382934687
98-99	22.818046982452834	25.152072434902895	26.21674879349472	25.81313178914955
100-101	23.169770865615337	24.92303819715372	26.268815040801258	25.63837589642969
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	69.0
1	41.0
2	34.5
3	67.0
4	60.0
5	52.0
6	105.5
7	228.5
8	357.0
9	408.5
10	365.5
11	316.0
12	332.5
13	361.5
14	438.5
15	538.0
16	592.5
17	638.0
18	711.5
19	846.0
20	999.0
21	1255.0
22	1610.0
23	2012.0
24	2602.5
25	3333.5
26	4516.0
27	6367.5
28	8633.5
29	12018.5
30	16487.5
31	22471.0
32	30523.0
33	36619.0
34	41304.0
35	48057.5
36	56311.5
37	64738.5
38	72705.5
39	80627.0
40	89847.5
41	99666.0
42	108323.5
43	117541.5
44	126799.0
45	134285.5
46	141194.0
47	148941.0
48	156496.5
49	162421.5
50	164009.0
51	161965.5
52	158896.0
53	155856.5
54	151253.5
55	141768.5
56	130425.5
57	118267.5
58	107058.5
59	95331.5
60	79784.5
61	65536.5
62	53346.5
63	40870.5
64	29909.0
65	21618.0
66	15796.5
67	11852.0
68	8954.5
69	6707.5
70	5051.0
71	3874.5
72	2906.5
73	2353.5
74	1829.5
75	1297.5
76	907.0
77	535.0
78	288.5
79	160.0
80	72.5
81	41.0
82	23.5
83	14.5
84	8.0
85	6.5
86	5.0
87	3.0
88	1.0
89	0.0
90	1.0
91	1.0
92	0.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18463096100090692
2	0.0
3	0.01202090621830756
4	0.013177847896595374
5	0.0071391766977272585
6	0.1088653901179121
7	0.14605683236140826
8	0.1269249675350878
9	0.14456127360654838
10-11	0.11706274517992701
12-13	0.11723205371821303
14-15	0.11929197426735962
16-17	0.06761054295555143
18-19	0.08112700792871885
20-21	0.038291947742355295
22-23	0.08613571885301366
24-25	0.05924387935525051
26-27	0.06751177964155125
28-29	0.04049295874007358
30-31	0.025509153101760638
32-33	0.03223916749863001
34-35	0.020683859760609016
36-37	0.0246767194551877
38-39	0.03305749210034578
40-41	0.026355695793190748
42-43	0.013248393120881219
44-45	0.026962384722048992
46-47	0.034778795572920344
48-49	0.008479535959158265
50-51	0.015421186028885165
52-53	0.006772341531440878
54-55	0.012726358461165982
56-57	0.005502527494295713
58-59	0.0036824607077209776
60-61	0.023787849629186085
62-63	0.010059748983161138
64-65	0.007012195294012742
66-67	0.0416499004183614
68-69	0.0925694433078825
70-71	0.021798474304325327
72-73	0.045543996798939904
74-75	0.0875466233387305
76-77	0.23845696713100475
78-79	0.08015348383357422
80-81	0.01717070759117406
82-83	0.011456544424020818
84-85	0.01322017503116688
86-87	0.25792744903389725
88-89	0.6257784665499944
90-91	0.27034340850820554
92-93	0.3564085821369333
94-95	0.001058178364287637
96-97	0.004303258681436391
98-99	0.003386170765720439
100-101	0.005220346597152343
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3543826.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.08261635556774
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.40635687104998	31.52343899970651
2	13.550439724456096	12.488794305427103
3	6.248955998207343	8.639047256646384
4	3.4343067000248304	6.330473524184003
5	2.1923688034507687	5.051504523966843
6	1.402078252801963	3.8766860534614547
7	1.002777586565398	3.234743036815875
8	0.702644139448284	2.590374425014664
9	0.5552916273101035	2.3030361924111356
>10	2.390715164943547	18.31724482474162
>50	0.08331777541398877	2.585514758613293
>100	0.028780016158129078	2.2620691111629148
>500	0.0013525463708881226	0.4207765714338014
>1k	6.147937986746483E-4	0.3762964164142914
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.821808971433699E-5	0.0
3	0.0	0.0	0.0	2.821808971433699E-5	0.0
4	0.0	0.0	0.0	8.465426914301097E-5	0.0
5	0.0	0.0	0.0	8.465426914301097E-5	0.0
6	0.0	0.0	0.0	8.465426914301097E-5	0.0
7	0.0	0.0	0.0	8.465426914301097E-5	0.0
8	0.0	0.0	0.0	8.465426914301097E-5	0.0
9	0.0	0.0	0.0	8.465426914301097E-5	0.0
10-11	0.0	0.0	0.0	1.1287235885734796E-4	0.0
12-13	0.0	0.0	0.0	1.4109044857168494E-4	0.0
14-15	0.0	0.0	0.0	1.9752662800035894E-4	4.2327134571505486E-5
16-17	0.0	0.0	0.0	2.1163567285752743E-4	8.465426914301097E-5
18-19	0.0	0.0	0.0	2.680718522862014E-4	8.465426914301097E-5
20-21	0.0	0.0	0.0	3.103989868577069E-4	8.465426914301097E-5
22-23	0.0	0.0	0.0	4.2327134571505486E-4	1.1287235885734796E-4
24-25	0.0	0.0	0.0	7.477793774299303E-4	1.5519949342885346E-4
26-27	0.0	0.0	0.0	0.0010863964540019741	1.6930853828602194E-4
28-29	0.0	0.0	0.0	0.004063404918864527	1.6930853828602194E-4
30-31	0.0	0.0	0.0	0.01686030860431635	1.6930853828602194E-4
32-33	0.0	0.0	0.0	0.04239767979579133	1.6930853828602194E-4
34-35	0.0	0.0	0.0	0.07600542464556669	1.9752662800035894E-4
36-37	0.0	0.0	0.0	0.13718224314624927	2.2574471771469591E-4
38-39	0.0	0.0	0.0	0.2437619679973001	2.2574471771469591E-4
40-41	0.0	0.0	0.0	0.36789334465066853	2.2574471771469591E-4
42-43	0.0	0.0	0.0	0.4998411321549082	2.2574471771469591E-4
44-45	0.0	0.0	0.0	0.6570158918637654	2.2574471771469591E-4
46-47	0.0	0.0	0.0	0.8467402180581101	2.3985376257186443E-4
48-49	0.0	0.0	0.0	1.0540020870099152	2.821808971433699E-4
50-51	0.0	0.0	0.0	1.2882263406837695	2.821808971433699E-4
52-53	0.0	0.0	0.0	1.5286867921844922	2.821808971433699E-4
54-55	0.0	2.821808971433699E-5	0.0	1.7961942826764068	3.103989868577069E-4
56-57	0.0	2.821808971433699E-5	0.0	2.105295237407254	3.103989868577069E-4
58-59	0.0	2.821808971433699E-5	0.0	2.4478205194047336	3.103989868577069E-4
60-61	0.0	2.821808971433699E-5	0.0	2.825900594442278	3.103989868577069E-4
62-63	0.0	2.821808971433699E-5	0.0	3.211782971285836	3.103989868577069E-4
64-65	0.0	2.821808971433699E-5	0.0	3.6115486482688484	3.103989868577069E-4
66-67	0.0	2.821808971433699E-5	0.0	4.065366076099673	3.103989868577069E-4
68-69	0.0	2.821808971433699E-5	0.0	4.575210521058314	3.386170765720439E-4
70-71	0.0	2.821808971433699E-5	0.0	5.128426169907891	3.386170765720439E-4
72-73	0.0	2.821808971433699E-5	0.0	5.697556821356353	3.950532560007179E-4
74-75	0.0	2.821808971433699E-5	0.0	6.273191742483971	3.950532560007179E-4
76-77	0.0	2.821808971433699E-5	0.0	6.890998598689665	3.950532560007179E-4
78-79	0.0	2.821808971433699E-5	0.0	7.5716894678237585	3.950532560007179E-4
80-81	0.0	2.821808971433699E-5	0.0	8.296569865450504	3.950532560007179E-4
82-83	0.0	2.821808971433699E-5	0.0	9.038931369655282	5.079256148580658E-4
84-85	0.0	2.821808971433699E-5	0.0	9.772827446945758	5.079256148580658E-4
86-87	0.0	2.821808971433699E-5	0.0	10.526687822709128	5.079256148580658E-4
88-89	0.0	2.821808971433699E-5	0.0	11.34063862051918	5.079256148580658E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4710	0.0	41.498333	1
GGTATCA	2965	0.0	41.220963	1
ATCAACG	5960	0.0	32.419197	3
TCAACGC	5950	0.0	32.314106	4
CAACGCA	6050	0.0	31.695696	5
TATCAAC	6195	0.0	31.111021	2
AACGCAG	6325	0.0	30.243008	6
GTACATG	10060	0.0	26.42552	1
TACATGG	10450	0.0	24.43968	2
ACATGGG	10285	0.0	23.540731	3
ACGCAGA	8155	0.0	23.048653	7
CGCAGAG	8305	0.0	22.460268	8
CATGGGG	7185	0.0	19.557756	4
GAGTACA	7850	0.0	18.96201	1
GTATAGG	980	0.0	18.440268	1
GCAGAGT	9925	0.0	18.076365	9
GTGTAGC	2220	0.0	17.351688	1
AGTACAT	7540	0.0	16.935986	2
GTATAAT	1080	0.0	16.732836	1
AGAGTAC	8375	0.0	16.603352	10-11
>>END_MODULE