FastQCFastQC Report
Mon 15 Aug 2016
SRR2920892_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920892_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4035923
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61850.043.607431
GGTATCA33350.042.6447141
TCAACGC77800.033.9002274
ATCAACG78200.033.7268263
CAACGCA78800.033.411815
AACGCAG83700.031.6687286
TATCAAC85600.030.9723242
ACGCAGA99500.026.1751357
GTACATG128350.026.0341451
TACATGG128900.025.1230012
CGCAGAG104800.024.456418
ACATGGG138050.022.9923863
GCAGAGT116350.021.0040479
TAAGGCG7100.020.6731195
CATGGGG98300.019.2246344
GTATAGG8750.018.9363841
AGAGTAC99150.018.54358910-11
TACCGTA2754.2595275E-818.3410267
CAGAGTA105150.017.52907210-11
AGTACAT93650.016.6529392
Produced by FastQC (version 0.11.5)