##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920892_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4035923
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.624488376017084	31.0	30.0	33.0	22.0	34.0
2	28.646586914567003	31.0	28.0	34.0	18.0	34.0
3	28.722298963582805	31.0	28.0	34.0	22.0	34.0
4	32.198255021218195	35.0	32.0	37.0	22.0	37.0
5	32.15346625790433	35.0	32.0	37.0	22.0	37.0
6	32.277757281296	35.0	32.0	37.0	23.0	37.0
7	32.11087228373782	35.0	32.0	37.0	22.0	37.0
8	32.047452590151	35.0	32.0	37.0	22.0	37.0
9	33.31820354352647	37.0	33.0	39.0	22.0	39.0
10-11	33.12054950007718	37.0	32.5	39.0	19.0	39.0
12-13	32.95138336385506	37.0	32.0	39.0	18.0	39.0
14-15	33.718052970782644	38.0	32.0	40.0	17.0	41.0
16-17	33.601596214793986	38.0	32.0	40.0	17.0	41.0
18-19	33.473334476401064	38.0	32.0	40.0	17.5	41.0
20-21	33.37330618051931	38.0	32.0	40.0	16.5	41.0
22-23	33.1460003077363	38.0	32.0	40.0	15.5	41.0
24-25	32.94796233723983	38.0	32.0	40.0	12.0	41.0
26-27	32.98833178928339	38.0	32.0	40.0	10.5	41.0
28-29	32.82851382447088	38.0	31.5	40.0	10.0	41.0
30-31	32.58502156755716	38.0	31.0	40.0	9.0	41.0
32-33	32.34142834241386	37.0	31.0	40.0	8.5	41.0
34-35	32.01813835397752	37.0	30.0	40.0	6.5	41.0
36-37	31.709911214857172	37.0	30.0	40.0	2.0	41.0
38-39	31.5054807784985	37.0	30.0	40.0	2.0	41.0
40-41	31.306834520876635	37.0	30.0	40.0	2.0	41.0
42-43	31.06954889872775	36.0	29.5	40.0	2.0	41.0
44-45	30.787001882840677	36.0	29.0	39.0	2.0	41.0
46-47	30.535859950747323	36.0	28.0	39.0	2.0	40.0
48-49	30.23199748855466	35.5	28.0	39.0	2.0	40.0
50-51	29.08401027472526	34.0	25.5	38.0	2.0	39.5
52-53	29.535169402389492	34.5	27.5	38.5	2.0	39.5
54-55	30.177368844747534	35.0	28.0	39.0	2.0	40.0
56-57	30.119092460386383	35.0	28.0	39.0	2.0	41.0
58-59	29.864151892887946	35.0	28.0	39.0	2.0	40.0
60-61	29.531636505453648	35.0	27.5	39.0	2.0	40.0
62-63	29.1267857439302	34.5	26.5	38.5	2.0	40.0
64-65	28.70206158541677	34.0	26.0	38.0	2.0	40.0
66-67	28.30797502826491	34.0	26.0	37.5	2.0	40.0
68-69	27.85448421587825	34.0	25.0	37.0	2.0	39.5
70-71	27.520994825718923	34.0	25.0	36.0	2.0	39.0
72-73	27.057357759303137	33.0	24.0	36.0	2.0	39.0
74-75	26.61874532789649	33.0	22.5	35.0	2.0	38.0
76-77	26.205337911550842	32.5	21.5	35.0	2.0	37.0
78-79	25.756206325046342	32.0	20.0	35.0	2.0	37.0
80-81	25.31794313221536	32.0	18.5	35.0	2.0	36.0
82-83	24.846451604750634	32.0	16.5	35.0	2.0	36.0
84-85	24.455702574107583	31.0	10.0	34.5	2.0	35.0
86-87	24.00758401485856	31.0	4.5	34.0	2.0	35.0
88-89	23.48645526686213	31.0	2.0	34.0	2.0	35.0
90-91	22.99148249359564	31.0	2.0	34.0	2.0	35.0
92-93	22.452097450818563	30.0	2.0	34.0	2.0	35.0
94-95	21.81472651980724	30.0	2.0	34.0	2.0	35.0
96-97	21.175834003770635	29.0	2.0	34.0	2.0	35.0
98	20.787950364761667	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	208424.0
3	35233.0
4	26030.0
5	21337.0
6	20065.0
7	20115.0
8	23066.0
9	26424.0
10	28889.0
11	31253.0
12	32251.0
13	32095.0
14	29918.0
15	30766.0
16	33224.0
17	35375.0
18	36801.0
19	37276.0
20	38560.0
21	41005.0
22	44183.0
23	49431.0
24	56823.0
25	65360.0
26	75953.0
27	86698.0
28	101375.0
29	116820.0
30	135588.0
31	159417.0
32	191219.0
33	232017.0
34	288275.0
35	365734.0
36	461751.0
37	501996.0
38	288126.0
39	27048.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.87650928263221	17.275419987813827	12.78506323132593	25.06300749822803
2	16.36872823702896	18.833641061227524	38.93731025167598	25.860320450067537
3	19.757214930508574	22.809499664698475	28.2560058128844	29.17727959190855
4	13.506230436567954	15.027739153082903	35.41444924021212	36.05158117013703
5	14.97449229316577	36.29068253096922	33.713022499073745	15.021802676791271
6	35.074598753820894	33.8264122363666	16.71055703343747	14.388431976375038
7	30.644729623037907	30.58847698077448	20.072702203468037	18.69409119271957
8	27.528657590730454	33.885959391215145	19.2831761481621	19.30220686989231
9	27.198338521324615	15.507307745960466	18.738563644549213	38.5557900881657
10-11	26.2913379983575	24.746052356581355	26.958174721231504	22.004434923829645
12-13	26.892429069567914	21.88758794097478	26.877314093538164	24.342668895919143
14-15	24.0053050233566	23.659883827174184	24.180189912898257	28.154621236570964
16-17	24.383256558011652	26.53928005186717	24.985866741366756	24.09159664875442
18-19	24.352139417178297	25.841428079246302	26.27912683324617	23.527305670329234
20-21	25.152494815176286	25.12433906515471	25.938636582291164	23.784529537377843
22-23	24.656157823853356	24.820336534115505	25.897554716833266	24.625950925197873
24-25	24.452756251044384	25.15004390742896	25.87885516399462	24.518344677532042
26-27	24.350818607579257	25.293592507100932	25.846901581997066	24.508687303322745
28-29	24.27857524281352	25.482974420353845	25.83116124582709	24.40728909100555
30-31	24.639517930181633	25.23666387080517	25.62247788358697	24.501340315426226
32-33	24.272920031859776	25.245812952179108	25.597631036220918	24.883635979740195
34-35	24.748482292521974	25.26233139656433	25.720906390989683	24.26827991992401
36-37	24.82817775285387	25.03274583405756	25.585577688806506	24.55349872428207
38-39	24.67694725532576	25.07374168698678	25.60950066476726	24.6398103929202
40-41	24.73490057184507	25.08430059753478	25.715503344143592	24.465295486476556
42-43	24.734661867270844	24.983286129764323	25.586129988253635	24.69592201471119
44-45	24.522308956230564	25.05740152937251	25.697763924216176	24.72252559018075
46-47	24.499344564863204	25.032647385846087	25.6033734388331	24.86463461045761
48-49	24.463842169819262	25.114706865896903	25.57110930291181	24.850341661372024
50-51	24.38805979800713	25.2446815707373	25.68022372023431	24.687034911021264
52-53	24.464717345823242	24.98420387662624	25.83948401238207	24.711594765168446
54-55	24.257499230706152	24.9461618738592	26.007382981167016	24.78895591426763
56-57	24.173848617634597	25.012097423255064	25.88899578574288	24.925058173367457
58-59	24.254199285905916	24.76312222819643	25.926040628513242	25.056637857384413
60-61	24.18603401441696	24.77725160694678	26.13611414251245	24.900600236123807
62-63	24.017337284177863	24.86686420889313	26.112748313998864	25.003050192930143
64-65	23.930037296486287	24.75631112441677	26.04462886549449	25.269022713602457
66-67	23.961909853885057	24.828687932511635	25.982172592956555	25.227229620646757
68-69	24.12328399248761	24.577875865459113	26.071248789884073	25.227591352169203
70-71	23.86247201103184	24.491038743358896	26.089740505322645	25.556748740286622
72-73	23.859476823546668	24.595770232000735	26.074349137363033	25.47040380708956
74-75	23.829936968209665	24.608338313365145	26.08297047876916	25.478754239656038
76-77	23.773064754389225	24.377485456255037	26.26729914841176	25.582150640943972
78-79	23.65892974399583	24.353339921668336	26.36261637358271	25.625113960753126
80-81	23.693381436875786	24.365719687222555	26.33187864123653	25.60902023466513
82-83	23.682838247192816	24.390963061432185	26.368804917866296	25.557393773508704
84-85	23.514207678530763	24.328424406538296	26.319495755198723	25.837872159732218
86-87	23.52363523463715	24.14979252379706	26.499718733213168	25.826853508352617
88-89	23.376164283441508	24.042048181430577	26.500271747476155	26.08151578765176
90-91	23.447678800037693	24.05878130568601	26.576637502849955	25.916902391426337
92-93	23.519161835710662	23.872793160417906	26.601244850878558	26.006800152992877
94-95	23.234463022243425	23.77141405184709	26.75861904359964	26.235503882309843
96-97	23.401736301764508	23.50619485329994	26.847762134340687	26.244306710594866
98	23.353333160963892	23.384409382731764	26.91688080255213	26.34537665375222
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	100.0
1	72.0
2	91.0
3	146.5
4	146.5
5	162.5
6	265.5
7	490.5
8	752.0
9	918.0
10	966.0
11	1051.5
12	1211.5
13	1355.0
14	1599.5
15	1839.5
16	1953.5
17	2055.0
18	2089.0
19	2000.0
20	1956.0
21	2020.5
22	2123.0
23	2348.5
24	2733.5
25	3292.5
26	4123.0
27	5366.5
28	6930.5
29	9471.5
30	13488.0
31	18641.0
32	24879.5
33	31772.5
34	40360.5
35	50396.5
36	61163.0
37	72705.0
38	84544.5
39	96180.0
40	106557.0
41	116024.0
42	127947.5
43	140512.0
44	149951.0
45	159734.0
46	169507.5
47	178294.5
48	187283.5
49	191240.0
50	190834.0
51	190759.0
52	189067.0
53	188527.0
54	184703.5
55	175395.5
56	162401.0
57	146935.0
58	133623.0
59	118821.5
60	101244.0
61	82913.0
62	66774.0
63	51502.0
64	38319.0
65	29678.0
66	22613.0
67	16684.5
68	12484.0
69	9869.5
70	7754.0
71	5992.0
72	4952.0
73	4189.5
74	3341.5
75	2455.0
76	1678.5
77	1058.5
78	592.5
79	292.5
80	177.5
81	115.5
82	69.5
83	42.5
84	32.0
85	23.0
86	15.5
87	12.0
88	10.0
89	8.5
90	9.0
91	6.5
92	6.0
93	4.0
94	1.5
95	3.0
96	3.5
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3709932027940077
2	0.02410848769909634
3	0.018558332257577758
4	0.016154916731563017
5	0.021506852335884504
6	0.02153162981553414
7	0.013801056164847546
8	0.00849867551982533
9	0.0
10-11	6.194369912409131E-5
12-13	0.004720109873255758
14-15	0.0031095736960293843
16-17	0.005686431579591583
18-19	0.03501057874493641
20-21	0.07430766146925993
22-23	0.0924571653126187
24-25	0.060382717906164216
26-27	0.06998399127039838
28-29	0.053742353360061626
30-31	0.04917090836470369
32-33	0.018360112420380666
34-35	0.32815294047978616
36-37	0.49914232754192783
38-39	0.4547039177903047
40-41	0.46460252091033455
42-43	0.4164103229917914
44-45	0.39092668517214035
46-47	0.388832988141746
48-49	0.3802971464024462
50-51	0.3762955834390299
52-53	0.4343987732174276
54-55	0.4367650225239679
56-57	0.4081346447888129
58-59	0.3930823259016587
60-61	0.4068833820665062
62-63	0.38864715704437375
64-65	0.4356376471999094
66-67	0.45593040303296173
68-69	0.4004907923169
70-71	0.3585920742293647
72-73	0.34572017355137846
74-75	0.3268397340583554
76-77	0.32571235873429694
78-79	0.32598491101044297
80-81	0.3410867848568964
82-83	0.3372091092917283
84-85	0.33675072591821004
86-87	0.3233956643870559
88-89	0.3648855540603723
90-91	0.3451502915194368
92-93	0.3348304712453632
94-95	0.3411487285560205
96-97	0.34077706636127597
98	0.3358587366508231
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	4035923.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.98606896283166
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.53988168440902	31.02772100400401
2	11.551841475913129	10.162401916144278
3	5.5170653586813705	7.28022052018225
4	3.3125953198536915	5.828321847401514
5	2.115628856592973	4.65290983929276
6	1.4806639394343868	3.907715169044336
7	1.0364129197173269	3.1911411112459236
8	0.7930590963672003	2.79068416835269
9	0.5987683014034634	2.370371741846128
>10	2.9072452304961303	21.61685502117234
>50	0.10265921523263781	3.0539079990251445
>100	0.04196393686260828	3.3505987141732647
>500	0.0018171610744446304	0.5249637522204238
>1k	3.975039615862882E-4	0.2421871958949458
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.4777479649636527E-5
2	0.0	0.0	0.0	0.0	2.4777479649636527E-5
3	0.0	0.0	0.0	0.0	2.4777479649636527E-5
4	0.0	0.0	0.0	0.0	2.4777479649636527E-5
5	2.4777479649636527E-5	0.0	0.0	2.4777479649636527E-5	2.4777479649636527E-5
6	4.9554959299273054E-5	0.0	0.0	2.4777479649636527E-5	2.4777479649636527E-5
7	7.433243894890957E-5	0.0	0.0	2.4777479649636527E-5	2.4777479649636527E-5
8	7.433243894890957E-5	0.0	0.0	2.4777479649636527E-5	2.4777479649636527E-5
9	7.433243894890957E-5	0.0	0.0	4.9554959299273054E-5	2.4777479649636527E-5
10-11	7.433243894890957E-5	0.0	0.0	4.9554959299273054E-5	2.4777479649636527E-5
12-13	1.4866487789781915E-4	2.4777479649636527E-5	0.0	7.433243894890957E-5	2.4777479649636527E-5
14-15	1.4866487789781915E-4	3.7166219474454794E-5	0.0	7.433243894890957E-5	4.9554959299273054E-5
16-17	1.4866487789781915E-4	6.194369912409131E-5	0.0	8.672117877372783E-5	7.433243894890957E-5
18-19	1.4866487789781915E-4	7.433243894890957E-5	0.0	1.2388739824818263E-4	7.433243894890957E-5
20-21	1.4866487789781915E-4	7.433243894890957E-5	0.0	2.106085770219105E-4	1.2388739824818263E-4
22-23	1.734423575474557E-4	7.433243894890957E-5	0.0	3.9643967439418443E-4	1.2388739824818263E-4
24-25	2.4777479649636526E-4	7.433243894890957E-5	0.0	8.300455682628237E-4	1.2388739824818263E-4
26-27	2.4777479649636526E-4	7.433243894890957E-5	0.0	0.0016477023967008289	1.362761380730009E-4
28-29	3.468847150949114E-4	7.433243894890957E-5	0.0	0.007098747919620865	1.4866487789781915E-4
30-31	3.468847150949114E-4	7.433243894890957E-5	0.0	0.02851887907673164	1.4866487789781915E-4
32-33	3.468847150949114E-4	7.433243894890957E-5	0.0	0.07118569903340574	1.6105361772263742E-4
34-35	3.592734549197296E-4	7.433243894890957E-5	0.0	0.12844645450371575	1.734423575474557E-4
36-37	3.9643967439418443E-4	7.433243894890957E-5	0.0	0.2220805500996922	1.734423575474557E-4
38-39	4.459946336934575E-4	7.433243894890957E-5	0.0	0.3785627228269717	1.9821983719709222E-4
40-41	4.5838337351827575E-4	7.433243894890957E-5	0.0	0.5594135467896686	1.9821983719709222E-4
42-43	4.8316085316791224E-4	7.433243894890957E-5	0.0	0.7441792125370083	2.2299731684672874E-4
44-45	4.955495929927305E-4	7.433243894890957E-5	0.0	0.9700383282832701	2.6016353632118353E-4
46-47	5.203270726423671E-4	7.433243894890957E-5	0.0	1.2307469691567456	3.0971849562045657E-4
48-49	5.698820319416402E-4	7.433243894890957E-5	0.0	1.5174224087030401	3.2210723544527485E-4
50-51	5.698820319416402E-4	7.433243894890957E-5	0.0	1.8263728024543582	3.716621947445479E-4
52-53	5.698820319416402E-4	7.433243894890957E-5	0.0	2.14150517737826	5.203270726423671E-4
54-55	5.946595115912766E-4	7.433243894890957E-5	0.0	2.492713562672033	5.203270726423671E-4
56-57	7.185469098394592E-4	7.433243894890957E-5	2.4777479649636527E-5	2.89820940587816	5.451045522920036E-4
58-59	7.185469098394592E-4	7.433243894890957E-5	2.4777479649636527E-5	3.342110342541223	5.698820319416402E-4
60-61	7.185469098394592E-4	7.433243894890957E-5	2.4777479649636527E-5	3.8155584236864777	5.946595115912766E-4
62-63	7.185469098394592E-4	7.433243894890957E-5	2.4777479649636527E-5	4.297579512790506	6.194369912409131E-4
64-65	7.433243894890958E-4	7.433243894890957E-5	2.4777479649636527E-5	4.80825575710934	6.194369912409131E-4
66-67	7.681018691387323E-4	7.433243894890957E-5	2.4777479649636527E-5	5.387602786277142	6.194369912409131E-4
68-69	7.928793487883689E-4	7.433243894890957E-5	2.4777479649636527E-5	6.027010426115662	6.194369912409131E-4
70-71	8.300455682628237E-4	7.433243894890957E-5	2.4777479649636527E-5	6.709097274650681	6.56603210715368E-4
72-73	8.672117877372784E-4	7.433243894890957E-5	3.7166219474454794E-5	7.401987104312942	7.06158170014641E-4
74-75	8.672117877372784E-4	7.433243894890957E-5	4.9554959299273054E-5	8.113373322533656	7.185469098394592E-4
76-77	8.672117877372784E-4	7.433243894890957E-5	4.9554959299273054E-5	8.874958714524535	7.185469098394592E-4
78-79	8.672117877372784E-4	7.433243894890957E-5	4.9554959299273054E-5	9.71262335777962	7.804906089635506E-4
80-81	8.672117877372784E-4	7.433243894890957E-5	4.9554959299273054E-5	10.578720158932665	8.176568284380054E-4
82-83	9.41544226686188E-4	9.910991859854611E-5	4.9554959299273054E-5	11.465159270878061	8.424343080876419E-4
84-85	9.787104461606428E-4	9.910991859854611E-5	4.9554959299273054E-5	12.362909302283517	8.424343080876419E-4
86	9.91099185985461E-4	9.910991859854611E-5	4.9554959299273054E-5	13.054783255280142	8.424343080876419E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6185	0.0	43.60743	1
GGTATCA	3335	0.0	42.644714	1
TCAACGC	7780	0.0	33.900227	4
ATCAACG	7820	0.0	33.726826	3
CAACGCA	7880	0.0	33.41181	5
AACGCAG	8370	0.0	31.668728	6
TATCAAC	8560	0.0	30.972324	2
ACGCAGA	9950	0.0	26.175135	7
GTACATG	12835	0.0	26.034145	1
TACATGG	12890	0.0	25.123001	2
CGCAGAG	10480	0.0	24.45641	8
ACATGGG	13805	0.0	22.992386	3
GCAGAGT	11635	0.0	21.004047	9
TAAGGCG	710	0.0	20.673119	5
CATGGGG	9830	0.0	19.224634	4
GTATAGG	875	0.0	18.936384	1
AGAGTAC	9915	0.0	18.543589	10-11
TACCGTA	275	4.2595275E-8	18.341026	7
CAGAGTA	10515	0.0	17.529072	10-11
AGTACAT	9365	0.0	16.652939	2
>>END_MODULE