##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920892_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4035923
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.282036352031493	31.0	30.0	34.0	27.0	34.0
2	30.29126596320098	31.0	30.0	34.0	26.0	34.0
3	30.4282410739749	31.0	30.0	34.0	27.0	34.0
4	34.076014582042326	35.0	35.0	37.0	30.0	37.0
5	33.60346567563355	35.0	35.0	37.0	28.0	37.0
6	33.54695889886898	35.0	35.0	37.0	28.0	37.0
7	33.35898405395742	35.0	35.0	37.0	27.0	37.0
8	33.2898221794618	35.0	33.0	37.0	27.0	37.0
9	34.63109306099249	38.0	35.0	39.0	27.0	39.0
10-11	34.43586337003951	37.5	34.0	39.0	27.0	39.0
12-13	34.25606335898876	37.0	34.0	39.0	26.5	39.0
14-15	35.160589659416196	38.0	34.0	40.0	26.0	41.0
16-17	35.030962805781975	38.0	34.0	40.0	25.5	41.0
18-19	34.932215133935905	38.5	34.0	40.0	25.0	41.0
20-21	34.81100519013866	38.0	34.0	40.0	25.0	41.0
22-23	34.6604412423131	38.0	34.0	40.0	24.5	41.0
24-25	34.48684960045075	38.0	33.0	40.0	24.0	41.0
26-27	34.30045543485344	38.0	33.0	40.0	23.0	41.0
28-29	34.24110606173606	38.0	33.0	40.0	23.5	41.0
30-31	34.23239194603069	38.0	33.0	40.0	23.0	41.0
32-33	34.08566503870366	38.0	33.0	40.0	22.5	41.0
34-35	33.920854287854354	38.0	33.0	40.0	21.5	41.0
36-37	33.724089384262285	38.0	33.0	40.0	19.5	41.0
38-39	33.53819423710512	38.0	32.0	40.0	18.5	41.0
40-41	33.31011629805623	38.0	32.0	40.0	17.5	41.0
42-43	33.34253936460136	38.0	32.0	40.0	17.5	41.0
44-45	33.204684405525086	38.0	32.0	40.0	15.5	41.0
46-47	32.963232326285706	37.5	32.0	40.0	14.5	41.0
48-49	32.70003478758143	37.0	31.0	40.0	12.5	41.0
50-51	32.431362293086366	37.0	31.0	40.0	9.0	41.0
52-53	32.162603696849516	37.0	31.0	40.0	8.0	41.0
54-55	31.86860638817936	36.0	30.5	39.5	7.5	41.0
56-57	31.53195316164357	36.0	30.0	39.0	6.5	41.0
58-59	31.179295913227286	35.5	29.5	39.0	2.0	40.0
60-61	30.83299247780495	35.0	29.0	39.0	2.0	40.0
62-63	30.494930651551083	35.0	29.0	38.5	2.0	40.0
64-65	30.08326769366016	34.5	28.0	38.0	2.0	40.0
66-67	29.63829587432664	34.0	28.0	37.5	2.0	40.0
68-69	29.18271433820715	34.0	28.0	37.0	2.0	39.0
70-71	28.752522904921626	34.0	26.5	36.0	2.0	39.0
72-73	28.310097591059098	34.0	26.0	36.0	2.0	39.0
74-75	27.868882409302657	33.0	26.0	35.0	2.0	38.0
76-77	26.43872962888539	31.5	24.5	34.0	2.0	36.0
78-79	27.28331226834605	33.0	26.0	35.0	2.0	37.0
80-81	27.31679172273604	33.0	26.0	35.0	2.0	36.0
82-83	26.98481784711948	33.0	25.0	35.0	2.0	36.0
84-85	26.67278798430991	33.0	25.0	35.0	2.0	35.5
86-87	26.14215001128614	32.0	24.0	35.0	2.0	35.0
88-89	25.780626761214226	32.0	22.5	35.0	2.0	35.0
90-91	25.422328424006107	32.0	20.0	34.5	2.0	35.0
92-93	25.118337366694064	32.0	19.5	34.0	2.0	35.0
94-95	24.734952079115484	32.0	17.5	34.0	2.0	35.0
96-97	24.197396481548335	31.5	6.0	34.0	2.0	35.0
98-99	23.589388350570616	31.0	2.0	34.0	2.0	35.0
100-101	21.397600623203168	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	94237.0
3	42550.0
4	23360.0
5	16429.0
6	14073.0
7	14290.0
8	15814.0
9	18269.0
10	20746.0
11	23048.0
12	25436.0
13	27763.0
14	29828.0
15	31746.0
16	33073.0
17	34487.0
18	34044.0
19	33553.0
20	33539.0
21	35066.0
22	37862.0
23	43151.0
24	50151.0
25	58897.0
26	69658.0
27	83316.0
28	98075.0
29	115687.0
30	137128.0
31	162151.0
32	194490.0
33	237873.0
34	301477.0
35	392158.0
36	519283.0
37	593749.0
38	319780.0
39	19686.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.017677300519075	18.52929129440736	13.443560979363657	25.009470425709914
2	16.47516565603457	19.88219299525784	37.69098171595444	25.951659632753156
3	19.467909664581374	23.691401482626897	28.639504585041646	28.20118426775008
4	13.943309769193348	16.030405150061867	34.98609420848503	35.040190872259764
5	14.978124863714035	36.62672909720212	33.6330963774695	14.762049661614341
6	34.95004581428125	34.38678068229793	16.455487717456606	14.20768578596421
7	30.490306181443906	30.791961676328146	20.035587259254996	18.682144882972953
8	27.52837686068675	34.18825916304728	19.20931062208668	19.074053354179295
9	27.003143333839525	15.455163077191822	18.636337018587056	38.9053565703816
10-11	26.256221649933998	24.95979160472769	26.93755968312547	21.84642706221284
12-13	26.88349875701231	21.930140408058165	26.95424666685437	24.232114168075157
14-15	23.952794412885904	23.799152744402132	24.112774249820088	28.135278592891876
16-17	24.430428707975	26.716375352855636	24.883125287460345	23.97007065170902
18-19	24.353601364270453	25.974205676863853	26.190875610627952	23.481317348237745
20-21	25.16469061068322	25.16127001328332	25.883511803415786	23.790527572617673
22-23	24.693463180677146	24.937683318546956	25.86068813388461	24.508165366891284
24-25	24.47606028173358	25.32312291451129	25.819859393528294	24.38095741022683
26-27	24.37645255552455	25.48583399234378	25.749186097815542	24.38852735431613
28-29	24.329416535622002	25.530334277912516	25.791423829174608	24.348825357290877
30-31	24.712833758147504	25.36688937759695	25.58758869330515	24.332688170950394
32-33	24.424089346410423	25.375921237072873	25.48978662879746	24.710202787719243
34-35	24.764466453569607	25.370623336160197	25.71967344503003	24.145236765240167
36-37	24.81149228812655	25.127955003755346	25.61665185222636	24.44390085589174
38-39	24.682904195917114	25.1581033129601	25.608628510534253	24.55036398058853
40-41	24.75543553689186	25.12279029514281	25.72219380370604	24.399580364259286
42-43	24.738621820426957	25.07898557970317	25.600732122817917	24.581660477051962
44-45	24.403670952617112	25.15480898609625	25.740177173106744	24.70134288817989
46-47	24.43611356734285	25.0586283754769	25.664710012151374	24.840548045028875
48-49	24.43688363847756	25.160212490007627	25.602466362934877	24.800437508579936
50-51	24.325293806278175	25.252793340391026	25.675350505735366	24.74656234759543
52-53	24.477988880084013	25.014148925994924	25.798138189661024	24.709724004260043
54-55	24.25932391849038	25.014081624106254	25.898082991008824	24.82851146639454
56-57	24.15554664894154	25.13497959772803	25.70292284924372	25.006550904086712
58-59	24.13121651431046	24.94104908044247	25.793376608815286	25.134357796431782
60-61	24.175283026349703	25.023407914824435	25.87135931954716	24.929949739278705
62-63	23.92949412740161	25.166694668000815	25.776690103535554	25.127121101062016
64-65	23.80227646950232	25.032875867118804	25.736631286030576	25.4282163773483
66-67	23.868296333992152	25.14931876238849	25.630400879868652	25.351984023750703
68-69	24.06010711518261	24.921522399116213	25.65422387745255	25.36414660824862
70-71	23.843485383290925	24.802600233332033	25.597378948940186	25.756535434436863
72-73	23.86804186577741	24.8973341521895	25.52296587885845	25.71165810317464
74-75	23.856393079899874	24.969171130265412	25.414408827825923	25.760026962008787
76-77	23.782420559600975	24.815708308475482	25.638240376968408	25.763630754955138
78-79	23.717997995356885	24.798717365654937	25.66620162781782	25.817083011170354
80-81	23.76318017222323	24.84688597521627	25.53056481566841	25.859369036892094
82-83	23.690809991041814	24.982337628224112	25.44663931714582	25.880213063588254
84-85	23.556978366616814	24.87116082937324	25.36198438776412	26.209876416245837
86-87	23.539243147073133	24.741695150532557	25.50260220748874	26.21645949490557
88-89	23.38388848090348	24.754839966735652	25.38944808030017	26.471823472060702
90-91	23.361582036701574	24.742390979892505	25.44681359569933	26.44921338770659
92-93	23.4218997936534	24.67052239868322	25.347896742901483	26.559681064761904
94-95	23.132649202973926	24.63639882077704	25.478557577805873	26.752394398443162
96-97	23.264945115026084	24.48957240627753	25.48921101200679	26.756271466689597
98-99	23.14827892261879	24.468046620906065	25.40033093925198	26.98334351722316
100-101	23.338588893299494	24.384633644603227	25.45928539251674	26.81749206958054
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	85.0
1	63.5
2	64.0
3	97.0
4	94.0
5	88.5
6	147.0
7	325.0
8	516.0
9	543.0
10	445.5
11	377.5
12	391.5
13	439.5
14	498.0
15	528.5
16	591.5
17	697.5
18	765.0
19	841.5
20	969.0
21	1132.5
22	1357.5
23	1694.5
24	2050.0
25	2450.0
26	3227.5
27	4278.0
28	5590.5
29	7613.0
30	10547.0
31	14830.5
32	20559.5
33	26621.5
34	33265.0
35	41582.5
36	51617.5
37	62173.0
38	72479.0
39	83416.5
40	94488.0
41	104517.5
42	114566.5
43	127304.5
44	139070.5
45	148290.5
46	158589.5
47	168344.5
48	176854.0
49	185915.5
50	189311.0
51	188556.0
52	189505.0
53	190160.5
54	186541.5
55	176970.0
56	163857.0
57	149131.0
58	135914.5
59	121329.0
60	103354.0
61	84839.0
62	68102.0
63	51845.0
64	38550.5
65	30174.5
66	23099.0
67	16782.0
68	12488.5
69	9713.0
70	7711.0
71	6046.5
72	4723.0
73	4321.5
74	3463.0
75	2393.5
76	1676.5
77	1066.5
78	626.0
79	322.0
80	175.0
81	101.0
82	53.5
83	28.0
84	13.5
85	8.5
86	4.5
87	1.5
88	1.5
89	3.0
90	3.0
91	3.0
92	2.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18813540297969014
2	0.0
3	0.01283473445851172
4	0.013726723725898636
5	0.007210246578044229
6	0.10973945736824017
7	0.14442792887773132
8	0.12601826149805137
9	0.1438828243254393
10-11	0.11738330984015304
12-13	0.1166647629303136
14-15	0.11868412752175897
16-17	0.06833628887369754
18-19	0.08155507426677863
20-21	0.038157318660440254
22-23	0.08660968011530447
24-25	0.05936684124052912
26-27	0.0676301307036829
28-29	0.041502278413141185
30-31	0.02531019546210371
32-33	0.033065546592439946
34-35	0.020540530629548682
36-37	0.024988088226658434
38-39	0.03217355732505303
40-41	0.024653592251388346
42-43	0.013243562872730723
44-45	0.02755255737039582
46-47	0.03470086024931596
48-49	0.008548230479124602
50-51	0.015139040065927919
52-53	0.006962471781547864
54-55	0.012933844377110267
56-57	0.005463434262744854
58-59	0.003729010687270297
60-61	0.023860712902599977
62-63	0.009935769339504248
64-65	0.007135914139095319
66-67	0.04173766446981273
68-69	0.09220939051612233
70-71	0.02231212042449769
72-73	0.04560295129515603
74-75	0.08876532084482286
76-77	0.2402672201625254
78-79	0.08139402064905599
80-81	0.017220348356497384
82-83	0.012586959662015356
84-85	0.013937332302920546
86-87	0.2576486221367454
88-89	0.6199944845330301
90-91	0.2671631743222058
92-93	0.3541073504127804
94-95	0.0011149865842336436
96-97	0.004075895402365209
98-99	0.003394514712000204
100-101	0.004881163490978396
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4035923.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.62258142551581
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.2398833829178	27.314576377647658
2	12.48811184698047	10.145986807098256
3	6.143678794287336	7.487162762194564
4	3.7489304981950284	6.091649376861085
5	2.368865002443087	4.811470572389951
6	1.650279483990795	4.022316762796458
7	1.208229352980351	3.4356976690499814
8	0.8827862669504071	2.8688845608416043
9	0.6918816290675155	2.5295416032252307
>10	3.40419356247123	23.299958844796524
>50	0.11935241967977177	3.267389877872358
>100	0.050806867270882634	3.730230015960015
>500	0.0023884656926485993	0.6280046834632457
>1k	6.124270726340146E-4	0.3671300858030412
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	4.9554959299273054E-5	0.0	0.0	0.0	0.0
3	4.9554959299273054E-5	0.0	0.0	0.0	0.0
4	4.9554959299273054E-5	0.0	0.0	0.0	0.0
5	7.433243894890957E-5	0.0	0.0	2.4777479649636527E-5	0.0
6	9.910991859854611E-5	0.0	0.0	2.4777479649636527E-5	0.0
7	9.910991859854611E-5	0.0	0.0	2.4777479649636527E-5	0.0
8	9.910991859854611E-5	0.0	0.0	2.4777479649636527E-5	0.0
9	9.910991859854611E-5	0.0	0.0	4.9554959299273054E-5	2.4777479649636527E-5
10-11	9.910991859854611E-5	0.0	0.0	4.9554959299273054E-5	2.4777479649636527E-5
12-13	9.910991859854611E-5	0.0	0.0	7.433243894890957E-5	6.194369912409131E-5
14-15	1.2388739824818263E-4	0.0	0.0	7.433243894890957E-5	7.433243894890957E-5
16-17	1.2388739824818263E-4	0.0	0.0	7.433243894890957E-5	9.910991859854611E-5
18-19	1.6105361772263742E-4	0.0	0.0	1.1149865842336436E-4	9.910991859854611E-5
20-21	1.9821983719709222E-4	0.0	0.0	2.35386056671547E-4	1.1149865842336437E-4
22-23	1.9821983719709222E-4	0.0	0.0	4.21217154043821E-4	1.4866487789781915E-4
24-25	2.4777479649636526E-4	0.0	0.0	8.548230479124602E-4	1.4866487789781915E-4
26-27	2.4777479649636526E-4	0.0	0.0	0.0016724798763504654	1.4866487789781915E-4
28-29	3.0971849562045657E-4	0.0	0.0	0.007247412797518684	1.4866487789781915E-4
30-31	3.716621947445479E-4	0.0	0.0	0.028940096230775463	1.4866487789781915E-4
32-33	3.716621947445479E-4	0.0	0.0	0.07178035854499701	1.9821983719709222E-4
34-35	3.8405093456936616E-4	0.0	0.0	0.12884289417810993	3.2210723544527485E-4
36-37	4.459946336934575E-4	0.0	0.0	0.22247698977408636	3.2210723544527485E-4
38-39	4.70772113343094E-4	0.0	0.0	0.37990070672805204	3.716621947445479E-4
40-41	4.70772113343094E-4	0.0	0.0	0.5618293510555082	3.716621947445479E-4
42-43	4.8316085316791224E-4	0.0	0.0	0.7478586682649793	3.716621947445479E-4
44-45	4.955495929927305E-4	0.0	0.0	0.9759601459195331	3.8405093456936616E-4
46-47	5.574932921168219E-4	1.2388739824818264E-5	0.0	1.2384775428074322	3.9643967439418443E-4
48-49	6.194369912409131E-4	2.4777479649636527E-5	0.0	1.5240875507287925	4.088284142190027E-4
50-51	6.194369912409131E-4	2.4777479649636527E-5	0.0	1.8291478801751173	4.459946336934575E-4
52-53	6.318257310657314E-4	2.4777479649636527E-5	0.0	2.141121126443691	5.574932921168219E-4
54-55	6.442144708905497E-4	2.4777479649636527E-5	0.0	2.4884022812129967	5.698820319416402E-4
56-57	7.185469098394592E-4	2.4777479649636527E-5	0.0	2.8906398858451956	5.946595115912766E-4
58-59	7.185469098394592E-4	2.4777479649636527E-5	0.0	3.3300684874314994	5.946595115912766E-4
60-61	7.185469098394592E-4	2.4777479649636527E-5	0.0	3.800605214717922	5.946595115912766E-4
62-63	7.433243894890958E-4	2.4777479649636527E-5	0.0	4.279442397686973	6.194369912409131E-4
64-65	7.433243894890958E-4	2.4777479649636527E-5	0.0	4.784432210426215	6.194369912409131E-4
66-67	8.176568284380054E-4	2.4777479649636527E-5	0.0	5.3580308643153005	6.442144708905497E-4
68-69	8.176568284380054E-4	2.4777479649636527E-5	0.0	5.987069128920448	6.937694301898227E-4
70-71	8.300455682628237E-4	2.4777479649636527E-5	0.0	6.661239572707408	7.433243894890958E-4
72-73	8.91989267386915E-4	2.4777479649636527E-5	0.0	7.347104986888997	8.052680886131871E-4
74-75	9.41544226686188E-4	2.4777479649636527E-5	0.0	8.056186899502295	9.043780072117332E-4
76-77	9.41544226686188E-4	2.4777479649636527E-5	0.0	8.817437795517902	9.41544226686188E-4
78-79	9.663217063358246E-4	2.4777479649636527E-5	0.0	9.650233664021837	9.91099185985461E-4
80-81	0.0010902091045840072	2.4777479649636527E-5	0.0	10.512279347252164	0.0010158766656350976
82-83	0.0010902091045840072	2.4777479649636527E-5	0.0	11.393688630828684	0.0010406541452847341
84-85	0.0011149865842336438	2.4777479649636527E-5	0.0	12.288366254757587	0.0010406541452847341
86-87	0.0011397640638832803	2.4777479649636527E-5	0.0	13.215304157190314	0.0010406541452847341
88-89	0.0011397640638832803	2.4777479649636527E-5	0.0	14.196678182413292	0.0010530428851095524
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3410	0.0	44.485523	1
GTATCAA	6020	0.0	41.392075	1
TCAACGC	7480	0.0	31.736195	4
ATCAACG	7530	0.0	31.714617	3
CAACGCA	7710	0.0	30.723307	5
TATCAAC	8250	0.0	29.405355	2
AACGCAG	8205	0.0	28.870157	6
GTACATG	13035	0.0	27.178635	1
TACATGG	13265	0.0	26.5556	2
ACATGGG	13810	0.0	24.821611	3
ACGCAGA	9975	0.0	23.41333	7
CGCAGAG	10460	0.0	22.099443	8
CATGGGG	9860	0.0	20.849585	4
GTATAGG	865	0.0	18.691545	1
AGTACAT	9350	0.0	18.380465	2
GCAGAGT	11980	0.0	18.265356	9
GAGTACA	10255	0.0	18.038328	1
GTATAGA	1065	0.0	16.967442	1
AGAGTAC	10150	0.0	16.71682	10-11
GTGGTAT	2165	0.0	16.473495	1
>>END_MODULE