FastQCFastQC Report
Mon 15 Aug 2016
SRR2920891_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920891_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3784021
Sequences flagged as poor quality0
Sequence length98
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31250.042.411591
GTATCAA54050.041.3792531
TCAACGC67450.032.6470764
ATCAACG68350.032.4856723
CAACGCA68300.031.9721075
AACGCAG71950.030.4087056
TATCAAC73400.030.1256182
GTACATG110950.027.7900661
TACATGG113850.026.352982
ACGCAGA85300.025.5934097
ACATGGG117600.025.473633
CGCAGAG92100.023.4031318
CATGGGG80150.021.2351514
GCAGAGT102250.020.3631749
GAGTACA82150.019.8306881
CATGGGA43700.018.476354
AGTACAT84400.018.0459882
AGAGTAC86400.017.94209510-11
GCACCGT8350.017.5781786
TAAGGCG10000.017.4328585
Produced by FastQC (version 0.11.5)