##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920891_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3784021
Sequences flagged as poor quality	0
Sequence length	98
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.9042568738387	31.0	30.0	33.0	23.0	34.0
2	28.930459159713966	31.0	30.0	34.0	22.0	34.0
3	29.003030110033745	31.0	30.0	34.0	23.0	34.0
4	32.50961794345222	35.0	33.0	37.0	25.0	37.0
5	32.48221455430612	35.0	33.0	37.0	25.0	37.0
6	32.60872600865587	35.0	33.0	37.0	25.0	37.0
7	32.454005144263206	35.0	33.0	37.0	25.0	37.0
8	32.40246367554514	35.0	33.0	37.0	25.0	37.0
9	33.70108384705053	37.0	33.0	39.0	25.0	39.0
10-11	33.50737139143784	37.0	33.0	39.0	23.0	39.0
12-13	33.344030860293856	37.0	33.0	39.0	22.0	39.0
14-15	34.138722010263685	38.0	33.0	40.0	21.5	41.0
16-17	34.03432975134123	38.0	33.0	40.0	20.0	41.0
18-19	33.92169810896927	38.0	33.0	40.0	19.0	41.0
20-21	33.83199313640172	38.0	33.0	40.0	18.0	41.0
22-23	33.6123513849421	38.0	32.0	40.0	18.0	41.0
24-25	33.425324542332085	38.0	32.0	40.0	17.5	41.0
26-27	33.471309752245034	38.0	32.0	40.0	17.0	41.0
28-29	33.31729805410699	38.0	32.0	40.0	16.0	41.0
30-31	33.07454570151698	38.0	32.0	40.0	15.5	41.0
32-33	32.84813601721555	38.0	31.5	40.0	12.0	41.0
34-35	32.52136431061032	37.5	31.0	40.0	9.0	41.0
36-37	32.22063804614192	37.0	30.0	40.0	8.5	41.0
38-39	32.02434856994715	37.0	30.0	40.0	8.0	41.0
40-41	31.83170495089747	37.0	30.0	40.0	6.5	41.0
42-43	31.604291968781357	37.0	30.0	40.0	2.0	41.0
44-45	31.328852165461026	36.5	30.0	40.0	2.0	41.0
46-47	31.07919366726559	36.0	29.5	39.0	2.0	40.5
48-49	30.78689454947528	36.0	29.0	39.0	2.0	40.0
50-51	29.627943660989196	34.5	27.0	38.0	2.0	39.5
52-53	30.092317405215248	35.0	28.0	38.5	2.0	39.5
54-55	30.758977553242964	36.0	29.0	39.0	2.0	40.0
56-57	30.711248695501425	36.0	29.0	39.0	2.0	41.0
58-59	30.472431971175638	35.5	28.5	39.0	2.0	41.0
60-61	30.154325385614932	35.0	28.0	39.0	2.0	40.0
62-63	29.765322919719527	35.0	28.0	39.0	2.0	40.0
64-65	29.34542686206023	34.5	27.5	38.0	2.0	40.0
66-67	28.964600883557466	34.0	26.5	38.0	2.0	40.0
68-69	28.520701259321765	34.0	26.0	37.0	2.0	40.0
70-71	28.19272448540851	34.0	26.0	37.0	2.0	39.0
72-73	27.729815849330645	34.0	26.0	36.0	2.0	39.0
74-75	27.281442016310166	33.0	25.0	36.0	2.0	39.0
76-77	26.862976315406286	33.0	24.0	35.0	2.0	37.5
78-79	26.39591363261462	33.0	23.0	35.0	2.0	37.0
80-81	25.93816881565932	32.0	21.5	35.0	2.0	36.5
82-83	25.467841748235543	32.0	20.0	35.0	2.0	36.0
84-85	25.0668361777062	32.0	18.5	35.0	2.0	35.5
86-87	24.6019307239574	31.5	14.5	34.0	2.0	35.0
88-89	24.070220540530826	31.0	7.0	34.0	2.0	35.0
90-91	23.569296391325523	31.0	2.0	34.0	2.0	35.0
92-93	23.02833322013805	31.0	2.0	34.0	2.0	35.0
94-95	22.39033543947034	30.0	2.0	34.0	2.0	35.0
96-97	21.74147487553584	30.0	2.0	34.0	2.0	35.0
98	21.340942082509585	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	168895.0
3	29179.0
4	21553.0
5	18172.0
6	16444.0
7	16969.0
8	19603.0
9	22930.0
10	24878.0
11	26857.0
12	28422.0
13	28677.0
14	26809.0
15	28023.0
16	30115.0
17	32283.0
18	33227.0
19	33362.0
20	35065.0
21	37335.0
22	40203.0
23	45108.0
24	52177.0
25	59906.0
26	68765.0
27	80328.0
28	93308.0
29	108584.0
30	126372.0
31	148662.0
32	177696.0
33	217139.0
34	270011.0
35	345587.0
36	442497.0
37	494506.0
38	303811.0
39	30563.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.720575801200276	17.51108880913457	12.391512141198387	25.376823248466767
2	16.420356473539385	19.50055112511465	38.55631652820254	25.522775873143424
3	19.286316170088146	23.25258315632999	28.909412701315972	28.551687972265892
4	13.383477963005252	15.270350705809715	35.930773345850994	35.415397985334046
5	14.694538249592416	36.563690112432255	34.01414020092746	14.727631437047876
6	34.43004013792538	34.34315613348999	17.03605805130835	14.19074567727628
7	30.110458686886414	31.05352827120737	20.551310216794203	18.284702825112014
8	27.09260491138268	33.88915890384675	19.963311442300093	19.05492474247048
9	27.272470210921135	15.315850519857053	19.04186049707441	38.369818772147404
10-11	26.02632570304521	25.0074887279284	27.483770238559753	21.48241533046663
12-13	26.661724587438794	22.273343230705905	27.35793669513648	23.706995486718824
14-15	23.505057919471025	23.78653868642749	24.812089587048344	27.89631380705314
16-17	24.17684355409178	26.969868273297426	25.275825825706775	23.577462346904014
18-19	24.001636929467164	26.086078380158895	26.626447514098423	23.28583717627552
20-21	24.666569696328573	25.537530826650094	26.380167802762333	23.415731674259003
22-23	24.426225280302603	25.286941915943935	26.25321302336654	24.03361978038692
24-25	24.27110349087617	25.438937696470198	26.313203643609935	23.976755169043702
26-27	23.92153502628603	25.804180297597583	26.149503825519854	24.124780850596533
28-29	24.100114515856518	25.96540621664306	26.247703270933513	23.686775996566904
30-31	24.101476337941588	25.63710575629683	26.08962465256318	24.171793253198402
32-33	23.98845095614501	25.69321289489046	26.059317637957008	24.259018511007525
34-35	24.549527278357136	25.692009714500674	25.848885486121365	23.90957752102082
36-37	24.473835435353777	25.32249952333431	25.977894927480428	24.225770113831484
38-39	24.202719843053004	25.53771692922932	26.19013207166294	24.069431156054737
40-41	24.335728770437555	25.40326937212754	26.1854918326753	24.075510024759605
42-43	24.33449856034829	25.297485786897056	26.01256874381682	24.355446908937832
44-45	24.215914694786946	25.495415961594937	26.013718292993815	24.274951050624306
46-47	24.112439495212275	25.47456152846494	25.88160519165365	24.531393784669138
48-49	24.27329205397015	25.454061501016955	25.849768864566414	24.422877580446485
50-51	23.999265827673206	25.57312122967499	26.061817946481806	24.36579499617
52-53	24.170255261876004	25.425207563073048	26.0252757942624	24.379261380788556
54-55	23.826161749820727	25.221506205039933	26.29851759496782	24.65381445017152
56-57	23.832554220310143	25.511241102570843	26.263163989948364	24.393040687170657
58-59	23.782717586023306	25.105679656477122	26.31959968665501	24.792003070844565
60-61	23.829657455315257	25.073110964568592	26.49965860545735	24.597572974658803
62-63	23.684305463743925	25.203896974796514	26.503357210841116	24.60844035061844
64-65	23.510593337008974	25.140055771515925	26.382846311191262	24.966504580283846
66-67	23.618882237256155	25.21696406127524	26.27800757512816	24.88614612634044
68-69	23.49696078629043	24.907757104851814	26.511409254957556	25.083872853900203
70-71	23.346451112372545	24.811232202722127	26.577325747218154	25.26499093768717
72-73	23.522740711396633	24.74615136998978	26.599298074796717	25.13180984381687
74-75	23.381200886910435	24.8513741027402	26.535811984743784	25.231613025605583
76-77	23.29722369726018	24.77022325638363	26.62565604577481	25.306897000581387
78-79	23.249322517652185	24.79006132998096	26.57817199859811	25.38244415376874
80-81	23.1419717046118	24.8009544218239	26.756954398490997	25.300119475073302
82-83	23.187814427170352	24.67580088823418	26.608076777200797	25.528307907394677
84-85	23.036352360905667	24.55062973480957	26.705631753810717	25.707386150474047
86-87	23.24933309915863	24.45392336522307	26.84820508402122	25.44853845159708
88-89	22.967952492901397	24.226440284021013	26.94655133158553	25.859055891492062
90-91	22.94302578667858	24.36139187953162	26.997307195188775	25.69827513860103
92-93	23.093388685460337	24.06759654917066	27.071787463103814	25.767227302265194
94-95	22.885203834409324	23.92784478137962	27.141582728586293	26.045368655624763
96-97	23.095868926700476	23.713968862335783	27.18095229256969	26.00920991839405
98	22.978118337713767	23.599377150391454	27.070202086752992	26.352302425141787
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	120.0
1	88.0
2	116.5
3	191.5
4	178.5
5	200.5
6	360.0
7	620.0
8	921.0
9	1131.5
10	1127.5
11	1131.0
12	1295.5
13	1482.0
14	1641.0
15	1782.0
16	1886.0
17	1966.0
18	2052.0
19	2106.0
20	2142.0
21	2239.5
22	2434.5
23	2770.0
24	3270.0
25	4026.0
26	5191.5
27	6836.0
28	9254.5
29	12972.5
30	17975.0
31	24915.0
32	32374.0
33	40360.0
34	49335.5
35	58791.0
36	69734.5
37	80523.0
38	88453.0
39	96806.0
40	108659.5
41	120178.5
42	130503.5
43	139080.5
44	144767.0
45	148708.5
46	153460.5
47	162825.5
48	168745.5
49	168643.0
50	168870.0
51	168162.5
52	167453.0
53	165150.0
54	159303.0
55	151228.0
56	138570.5
57	124373.5
58	111839.0
59	95897.0
60	80504.5
61	68869.5
62	59250.5
63	47965.0
64	35964.0
65	27934.0
66	22945.5
67	18968.5
68	13903.0
69	10453.5
70	9014.5
71	8855.0
72	7723.0
73	5175.5
74	3310.5
75	1903.5
76	1132.5
77	684.0
78	438.5
79	274.0
80	157.5
81	82.0
82	54.0
83	37.5
84	27.5
85	24.5
86	19.0
87	12.0
88	9.5
89	9.0
90	6.0
91	4.5
92	4.0
93	3.0
94	5.5
95	5.5
96	2.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3581639742485573
2	0.022489304366968367
3	0.017573898242108064
4	0.014931206777129408
5	0.020216589707086723
6	0.021564362354225836
7	0.013874130191137944
8	0.0073995361019402375
9	0.0
10-11	1.3213457324893282E-5
12-13	0.0049814734114847666
14-15	0.0027351856662529094
16-17	0.005338236759256885
18-19	0.035121369569566345
20-21	0.07388965336080323
22-23	0.09271883004877615
24-25	0.060174084657564
26-27	0.06975384121811164
28-29	0.05317095227537057
30-31	0.049114420876628324
32-33	0.017904234675230394
34-35	0.31522023794265414
36-37	0.4830575728834486
38-39	0.43959851174187453
40-41	0.449204695217072
42-43	0.4024158428296249
44-45	0.37816914863844575
46-47	0.37732348736965254
48-49	0.3683119094740753
50-51	0.3644007261059069
52-53	0.4214960752067708
54-55	0.4216414232373446
56-57	0.3933514111047481
58-59	0.37986047117603206
60-61	0.3943292069467902
62-63	0.37592286089321386
64-65	0.42199818658511673
66-67	0.4411180593342373
68-69	0.3864936267531285
70-71	0.34842565620011096
72-73	0.3350668508446438
74-75	0.318457534987253
76-77	0.31685870665094085
78-79	0.3162508876139958
80-81	0.33094425215927714
82-83	0.3271784168216825
84-85	0.32647810358346324
86-87	0.3147049131069833
88-89	0.35371103913006824
90-91	0.3349479297287198
92-93	0.3247867810458768
94-95	0.3314067231656484
96-97	0.33148600390959776
98	0.32568529614396957
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3784021.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.19076810645069
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.29939209738687	35.32354006908975
2	12.421349030703439	11.97188701415831
3	5.277476610077036	7.629769545103199
4	2.8246140198451277	5.444812768823443
5	1.6531789551735572	3.9833981833616665
6	1.0523848156675202	3.0429139566349996
7	0.7203454757762207	2.4299801245763915
8	0.49586694848465185	1.9116967300861751
9	0.36707455041240206	1.5920644083033337
>10	1.6911008069602596	14.15807386052251
>50	0.11527229806616837	3.834437356022126
>100	0.07724012425711986	6.888611890004423
>500	0.003874102401996997	1.2361406875859224
>1k	8.301647875326645E-4	0.5526734057277967
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	3.9640371974679845E-5	0.0	0.0	0.0	0.0
16-17	7.928074394935969E-5	0.0	0.0	0.0	0.0
18-19	7.928074394935969E-5	0.0	0.0	1.3213457324893282E-5	0.0
20-21	1.0570765859914626E-4	0.0	0.0	1.1892111592403954E-4	0.0
22-23	1.0570765859914626E-4	0.0	0.0	2.642691464978656E-4	0.0
24-25	1.0570765859914626E-4	0.0	0.0	9.249420127425297E-4	0.0
26-27	1.0570765859914626E-4	0.0	0.0	0.0020216589707086723	0.0
28-29	1.0570765859914626E-4	0.0	0.0	0.007862007108311503	0.0
30-31	1.0570765859914626E-4	2.6426914649786564E-5	0.0	0.029571717493111164	0.0
32-33	1.0570765859914626E-4	2.6426914649786564E-5	0.0	0.07555454898373978	0.0
34-35	1.7177494522361268E-4	2.6426914649786564E-5	0.0	0.13748602346551458	0.0
36-37	2.1141531719829252E-4	2.6426914649786564E-5	0.0	0.23636232462769102	0.0
38-39	2.1141531719829252E-4	2.6426914649786564E-5	0.0	0.40073773374936345	0.0
40-41	2.1141531719829252E-4	2.6426914649786564E-5	0.0	0.589861948440561	0.0
42-43	2.1141531719829252E-4	2.6426914649786564E-5	0.0	0.7862271377458001	0.0
44-45	2.1141531719829252E-4	2.6426914649786564E-5	0.0	1.0266988476015328	0.0
46-47	3.435498904472253E-4	2.6426914649786564E-5	0.0	1.3229181339109903	0.0
48-49	4.756844636961581E-4	2.6426914649786564E-5	0.0	1.6366981050052312	0.0
50-51	4.756844636961581E-4	2.6426914649786564E-5	0.0	1.9805783318855787	0.0
52-53	4.756844636961581E-4	2.6426914649786564E-5	0.0	2.328858640055116	0.0
54-55	5.021113783459447E-4	2.6426914649786564E-5	0.0	2.7195409327802356	0.0
56-57	5.021113783459447E-4	2.6426914649786564E-5	0.0	3.180703806876336	0.0
58-59	5.021113783459447E-4	3.9640371974679845E-5	0.0	3.6698659970438854	0.0
60-61	5.021113783459447E-4	5.285382929957313E-5	0.0	4.195999440806486	0.0
62-63	5.021113783459447E-4	5.285382929957313E-5	0.0	4.7270747176085965	0.0
64-65	5.021113783459447E-4	5.285382929957313E-5	0.0	5.294804125029962	0.0
66-67	5.285382929957312E-4	5.285382929957313E-5	0.0	5.943333823993049	0.0
68-69	5.549652076455178E-4	5.285382929957313E-5	0.0	6.647333616806038	0.0
70-71	5.549652076455178E-4	5.285382929957313E-5	0.0	7.402073085746617	0.0
72-73	5.549652076455178E-4	5.285382929957313E-5	0.0	8.153786672959797	0.0
74-75	5.549652076455178E-4	5.285382929957313E-5	0.0	8.934768068147614	0.0
76-77	5.681786649704111E-4	5.285382929957313E-5	0.0	9.789943026214706	0.0
78-79	5.813921222953044E-4	5.285382929957313E-5	0.0	10.706217010952106	0.0
80-81	5.813921222953044E-4	5.285382929957313E-5	0.0	11.669266634619628	0.0
82-83	5.813921222953044E-4	5.285382929957313E-5	0.0	12.634192569227285	0.0
84-85	6.474594089197708E-4	5.285382929957313E-5	0.0	13.607905452955997	0.0
86	6.870997808944506E-4	5.285382929957313E-5	0.0	14.355655002971707	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3125	0.0	42.41159	1
GTATCAA	5405	0.0	41.379253	1
TCAACGC	6745	0.0	32.647076	4
ATCAACG	6835	0.0	32.485672	3
CAACGCA	6830	0.0	31.972107	5
AACGCAG	7195	0.0	30.408705	6
TATCAAC	7340	0.0	30.125618	2
GTACATG	11095	0.0	27.790066	1
TACATGG	11385	0.0	26.35298	2
ACGCAGA	8530	0.0	25.593409	7
ACATGGG	11760	0.0	25.47363	3
CGCAGAG	9210	0.0	23.403131	8
CATGGGG	8015	0.0	21.235151	4
GCAGAGT	10225	0.0	20.363174	9
GAGTACA	8215	0.0	19.830688	1
CATGGGA	4370	0.0	18.47635	4
AGTACAT	8440	0.0	18.045988	2
AGAGTAC	8640	0.0	17.942095	10-11
GCACCGT	835	0.0	17.578178	6
TAAGGCG	1000	0.0	17.432858	5
>>END_MODULE