FastQCFastQC Report
Mon 15 Aug 2016
SRR2920891_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920891_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3784021
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30400.047.704511
GTATCAA55400.044.9732971
TCAACGC70750.034.8262564
ATCAACG71350.034.7995453
CAACGCA73500.033.453775
TATCAAC76750.032.4722442
AACGCAG77250.032.0755926
ACGCAGA91050.026.7448437
GTACATG115200.025.9203191
TACATGG115100.025.2822972
CGCAGAG97900.024.6785988
ACATGGG118650.024.2076993
GCAGAGT107400.021.1695149
CATGGGG83900.020.8799884
AGAGTAC93350.018.58236510-11
GAGTACA88000.017.7765051
TATTAAG21200.017.6897872
CAGAGTA103600.017.0874410-11
AGTACAT88550.016.8870332
GTATTAA19700.016.6539021