##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920891_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3784021
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.32069589465809	31.0	30.0	34.0	27.0	34.0
2	30.32739987436645	31.0	30.0	34.0	26.0	34.0
3	30.464310848169184	31.0	30.0	34.0	27.0	34.0
4	34.120330463282315	35.0	35.0	37.0	30.0	37.0
5	33.65816918035075	35.0	35.0	37.0	28.0	37.0
6	33.60567000024577	35.0	35.0	37.0	28.0	37.0
7	33.42138851766415	35.0	35.0	37.0	28.0	37.0
8	33.35704928698863	35.0	35.0	37.0	27.0	37.0
9	34.702339918303835	38.0	35.0	39.0	27.0	39.0
10-11	34.505898619484405	38.0	34.0	39.0	27.0	39.0
12-13	34.32731557250871	37.0	34.0	39.0	27.0	39.0
14-15	35.24150724850629	38.0	34.0	40.0	27.0	41.0
16-17	35.12121721840339	38.0	34.0	40.0	26.0	41.0
18-19	35.02260188302338	38.5	34.0	40.0	25.5	41.0
20-21	34.907755268800045	38.0	34.0	40.0	25.0	41.0
22-23	34.757454702286275	38.0	34.0	40.0	25.0	41.0
24-25	34.576239666746034	38.0	33.5	40.0	24.0	41.0
26-27	34.40704927377517	38.0	33.0	40.0	24.0	41.0
28-29	34.345141451382005	38.0	33.0	40.0	23.5	41.0
30-31	34.33590326269331	38.0	33.0	40.0	24.0	41.0
32-33	34.19221523876321	38.0	33.0	40.0	23.0	41.0
34-35	34.012664702442194	38.0	33.0	40.0	22.5	41.0
36-37	33.822052916725355	38.0	33.0	40.0	20.5	41.0
38-39	33.63687661881369	38.0	33.0	40.0	19.0	41.0
40-41	33.409048205599284	38.0	32.0	40.0	18.0	41.0
42-43	33.43981838367176	38.0	32.0	40.0	18.0	41.0
44-45	33.29844073275492	38.0	32.0	40.0	16.5	41.0
46-47	33.05375102305193	38.0	32.0	40.0	15.0	41.0
48-49	32.79527373130328	37.0	31.0	40.0	13.5	41.0
50-51	32.53330491559112	37.0	31.0	40.0	10.5	41.0
52-53	32.270085181873995	37.0	31.0	40.0	8.5	41.0
54-55	31.987603662876076	36.5	30.5	40.0	8.0	41.0
56-57	31.658005729883634	36.0	30.0	39.0	7.0	41.0
58-59	31.330818592180115	36.0	30.0	39.0	3.5	40.5
60-61	30.990844131150435	35.0	29.0	39.0	2.0	40.0
62-63	30.66572106761564	35.0	29.0	39.0	2.0	40.0
64-65	30.25627606189289	35.0	28.0	38.0	2.0	40.0
66-67	29.820897399882295	34.0	28.0	38.0	2.0	40.0
68-69	29.383528262660278	34.0	28.0	37.0	2.0	39.5
70-71	28.959366240303638	34.0	27.0	36.5	2.0	39.0
72-73	28.51735481911966	34.0	26.0	36.0	2.0	39.0
74-75	28.077010407711796	33.0	26.0	36.0	2.0	38.5
76-77	26.632080530208473	31.5	24.5	34.5	2.0	36.0
78-79	27.48453351606664	33.0	26.0	35.0	2.0	37.0
80-81	27.514262737971066	33.0	26.0	35.0	2.0	36.5
82-83	27.182529510274918	33.0	26.0	35.0	2.0	36.0
84-85	26.869102867029543	33.0	25.0	35.0	2.0	36.0
86-87	26.329011519756364	32.5	24.0	35.0	2.0	35.0
88-89	25.97259489310445	32.0	23.5	35.0	2.0	35.0
90-91	25.61569848581707	32.0	21.5	35.0	2.0	35.0
92-93	25.3071371696933	32.0	20.0	34.0	2.0	35.0
94-95	24.92728700501398	32.0	18.5	34.0	2.0	35.0
96-97	24.399773944172086	32.0	11.5	34.0	2.0	35.0
98-99	23.80093186586438	31.5	2.0	34.0	2.0	35.0
100-101	21.60821966897118	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	83489.0
3	38389.0
4	20652.0
5	14807.0
6	12629.0
7	12649.0
8	14259.0
9	16712.0
10	18982.0
11	21145.0
12	23713.0
13	25670.0
14	27905.0
15	29708.0
16	31440.0
17	32631.0
18	31719.0
19	30738.0
20	30920.0
21	32350.0
22	35161.0
23	39980.0
24	46934.0
25	55250.0
26	64992.0
27	77991.0
28	91889.0
29	108345.0
30	127796.0
31	151122.0
32	181620.0
33	221510.0
34	278874.0
35	361766.0
36	482119.0
37	562086.0
38	324364.0
39	21714.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.76944045618562	18.832086022484688	13.137020099500695	25.261453421828996
2	16.46050590099791	20.599727115679325	37.46752462525975	25.472242358063024
3	19.032463197222924	24.15821031381672	29.376066790254434	27.433259698705925
4	13.711511568499807	16.337725818929368	35.50346279258899	34.44729981998183
5	14.621476649128537	36.87124645458111	34.08385741575302	14.423419480537328
6	34.27073362889499	34.93535051781492	16.760425215123487	14.033490638166606
7	29.827796650360618	31.30502763489545	20.62589518323931	18.24128053150463
8	27.055215616751887	34.210715018092095	19.933479304893133	18.80059006026288
9	27.010975755312614	15.295555153878379	18.911655398386866	38.78181369242214
10-11	25.965698762018114	25.165520862395148	27.553963525021235	21.314816850565506
12-13	26.581614699773787	22.357969653274772	27.467291945021366	23.59312370193007
14-15	23.420572769908336	23.95652923935297	24.772885899505713	27.850012091232983
16-17	24.128668638364758	27.17481790352642	25.258163930195117	23.4383495279137
18-19	24.020842152118036	26.177842505067783	26.636161440722628	23.165153902091554
20-21	24.605581076510035	25.601543797073006	26.363343517187747	23.42953160922921
22-23	24.295433602515423	25.462305162138744	26.32840990722687	23.913851328118966
24-25	24.23062969585334	25.57923567492042	26.35082714711589	23.839307482110343
26-27	23.83885778417619	25.9370111056694	26.219809535495514	24.004321574658892
28-29	23.985066496338465	26.000478783683235	26.308423142727104	23.706031577251192
30-31	23.984736938725586	25.701415009117472	26.183450936865476	24.130397115291462
32-33	23.87303395810932	25.800126757203117	26.109089178781186	24.217750105906372
34-35	24.433654242001126	25.785779091750715	25.96492317817865	23.815643488069508
36-37	24.388683378333603	25.415651688002423	26.017838953531943	24.177825980132038
38-39	24.134242420317207	25.617254007105238	26.234237053982945	24.014266518594614
40-41	24.257538215950603	25.426997387177387	26.284421912500513	24.031042484371504
42-43	24.27410444237496	25.410021618582864	26.087044430885754	24.228829508156426
44-45	24.118903531443767	25.51068272676219	26.078446481423008	24.291967260371035
46-47	24.083156488769074	25.45829453327244	25.974162959594842	24.484386018363637
48-49	24.200642311443747	25.46416139743374	25.9302686525824	24.404927638540112
50-51	23.93650924546746	25.535685235308474	26.14314692006512	24.384658599158943
52-53	24.121827362871603	25.448186013961433	26.04519474636544	24.384791876801522
54-55	23.819518173487886	25.263528194735063	26.214774917068866	24.702178714708182
56-57	23.76489489006958	25.61639632458809	26.09515498508397	24.52355380025836
58-59	23.684856272282886	25.312223043397196	26.19782347374985	24.805097210570068
60-61	23.76647713808449	25.365220621869266	26.267429715144104	24.600872524902144
62-63	23.542607119070524	25.52095336765654	26.196585827404302	24.739853685868635
64-65	23.29476627123502	25.4203396598961	26.066879849926877	25.218014218942002
66-67	23.49697788150842	25.427435051286917	25.99321973135902	25.08236733584564
68-69	23.335195489350266	25.137439810567948	26.225333045545586	25.3020316545362
70-71	23.19984806519029	24.97553989656644	26.191560243354733	25.633051794888534
72-73	23.326740322259965	24.943354392896573	26.185287846932866	25.5446174379106
74-75	23.121707207434177	25.020588115879583	26.03471235026419	25.82299232642205
76-77	23.011430234932902	24.946962554994315	26.278746109770623	25.76286110030216
78-79	22.916631677891505	25.002998565581443	26.106510371863127	25.97385938466393
80-81	22.71563989122406	25.002686094830445	26.223607053334806	26.05806696061068
82-83	22.701442850967688	24.89253910080795	26.085124810844107	26.320893237380254
84-85	22.474941430450247	24.73052985355498	26.20613362737926	26.588395088615517
86-87	22.606834963093117	24.70729697969085	26.26259080340973	26.423277253806305
88-89	22.273146926932206	24.530855123765114	26.301309978642113	26.894687970660563
90-91	22.093941254753734	24.60309101134537	26.349550179749244	26.953417554151653
92-93	22.16689261725032	24.322254329128317	26.427387120180253	27.083465933441108
94-95	21.839966987101263	24.212498204599896	26.494331160442236	27.4532036478566
96-97	21.916832920308984	24.08458308766379	26.493408744523144	27.50517524750408
98-99	21.631017728074386	24.07296044161686	26.448279010606345	27.847742819702404
100-101	21.639309169232394	23.87313186304954	26.63222643603737	27.855332531680695
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	117.0
1	86.0
2	89.0
3	123.0
4	104.0
5	112.0
6	216.5
7	441.0
8	674.0
9	691.0
10	543.5
11	505.0
12	523.0
13	570.5
14	669.5
15	710.5
16	758.5
17	847.0
18	915.0
19	990.5
20	1146.0
21	1383.0
22	1673.5
23	2061.5
24	2571.0
25	3280.0
26	4285.5
27	5714.0
28	7657.0
29	10533.5
30	14623.0
31	20147.0
32	27420.0
33	34905.0
34	42415.0
35	50513.5
36	59889.5
37	70361.0
38	79660.5
39	87513.0
40	97093.0
41	108664.0
42	120174.5
43	130741.0
44	137928.0
45	143272.0
46	147909.5
47	153411.0
48	162242.0
49	167278.0
50	167211.5
51	166344.0
52	164583.0
53	163619.5
54	160804.0
55	152664.5
56	139079.0
57	124484.5
58	111631.5
59	96797.0
60	82288.0
61	69697.5
62	59511.0
63	48160.0
64	36591.0
65	29120.5
66	22827.5
67	18284.5
68	14220.5
69	10367.0
70	8258.0
71	8419.0
72	8416.5
73	5938.0
74	3485.0
75	2227.5
76	1258.5
77	701.5
78	420.5
79	227.5
80	131.0
81	70.0
82	31.0
83	17.0
84	12.0
85	6.0
86	4.5
87	5.0
88	2.0
89	3.5
90	3.5
91	2.0
92	1.0
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18398417979181406
2	0.0
3	0.012552784458648618
4	0.013583434129990293
5	0.007003132382193439
6	0.10832392314947513
7	0.14442308856108357
8	0.12542213692788703
9	0.14323387740184315
10-11	0.1152609882450441
12-13	0.11551204393421706
14-15	0.11688624349600596
16-17	0.06630512885631448
18-19	0.08006033793152839
20-21	0.0369844670523763
22-23	0.0841697231595702
24-25	0.05885273892507468
26-27	0.06683366714931022
28-29	0.04015569681035068
30-31	0.0237974366421328
32-33	0.0316726572077692
34-35	0.019912680188614175
36-37	0.023665302068883867
38-39	0.031130905457448572
40-41	0.025039501630672766
42-43	0.012433863342724578
44-45	0.026995093314756972
46-47	0.033654675806503186
48-49	0.009051218267551897
50-51	0.015129408637002809
52-53	0.006355672973273668
54-55	0.01215638073890182
56-57	0.004571856234413075
58-59	0.003897969910843518
60-61	0.02279321388544091
62-63	0.01006865448156868
64-65	0.007016345839518333
66-67	0.04162239057341384
68-69	0.09135784394431215
70-71	0.02165685655550009
72-73	0.04579784308808012
74-75	0.08775057009461629
76-77	0.23408960996780936
78-79	0.08050959548057476
80-81	0.017111427235736797
82-83	0.011508921329982049
84-85	0.012817053605146483
86-87	0.25342089803412826
88-89	0.6170420301578664
90-91	0.2646259098456377
92-93	0.3510815611224145
94-95	0.0012024246165652887
96-97	0.003805475709569265
98-99	0.0028408933248520556
100-101	0.004519002405113503
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3784021.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.87669430417195
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.21027168506106	32.668918654125775
2	13.215702094795958	12.125854500359182
3	5.748679675836075	7.911912604228137
4	3.116392457979817	5.718791365062684
5	1.792504128899544	4.111708198024521
6	1.159768332509323	3.1923802352513677
7	0.7726299351921199	2.4811995142942744
8	0.5546501503796595	2.0356412315784516
9	0.3902942767837304	1.6114870102207568
>10	1.818194925661347	14.524874592559167
>50	0.12757370745978677	4.026307051835365
>100	0.08729756083079605	7.391545528878061
>500	0.004821237464582117	1.4074131581023248
>1k	0.0012198311463376952	0.7919663554798814
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	2.6426914649786564E-5	0.0	0.0	0.0	0.0
6	2.6426914649786564E-5	0.0	0.0	0.0	0.0
7	2.6426914649786564E-5	0.0	0.0	0.0	0.0
8	2.6426914649786564E-5	0.0	0.0	0.0	0.0
9	2.6426914649786564E-5	0.0	0.0	0.0	0.0
10-11	2.6426914649786564E-5	0.0	0.0	0.0	0.0
12-13	6.60672866244664E-5	0.0	0.0	0.0	0.0
14-15	1.1892111592403954E-4	0.0	0.0	2.6426914649786564E-5	0.0
16-17	1.321345732489328E-4	0.0	0.0	2.6426914649786564E-5	0.0
18-19	1.321345732489328E-4	0.0	0.0	3.9640371974679845E-5	0.0
20-21	1.5856148789871938E-4	0.0	0.0	1.453480305738261E-4	0.0
22-23	1.5856148789871938E-4	2.6426914649786564E-5	0.0	3.039095184725455E-4	0.0
24-25	1.5856148789871938E-4	2.6426914649786564E-5	0.0	9.777958420421028E-4	0.0
26-27	1.9820185987339922E-4	2.6426914649786564E-5	0.0	0.002074512800008245	0.0
28-29	2.3784223184807906E-4	2.6426914649786564E-5	0.0	0.00776951290703725	0.0
30-31	2.642691464978656E-4	2.6426914649786564E-5	0.0	0.029254594517313725	0.0
32-33	2.642691464978656E-4	2.6426914649786564E-5	0.0	0.07517135872131789	0.0
34-35	3.435498904472253E-4	2.6426914649786564E-5	0.0	0.13660072182474675	0.0
36-37	3.699768050970119E-4	2.6426914649786564E-5	0.0	0.23591306707864468	0.0
38-39	3.699768050970119E-4	2.6426914649786564E-5	0.0	0.4007641606640132	0.0
40-41	3.699768050970119E-4	2.6426914649786564E-5	0.0	0.5909983057705017	0.0
42-43	3.9640371974679844E-4	2.6426914649786564E-5	0.0	0.7887905484668294	0.0
44-45	4.0961717707169176E-4	2.6426914649786564E-5	0.0	1.030002211932756	0.0
46-47	4.756844636961581E-4	2.6426914649786564E-5	0.0	1.3269085980231081	0.0
48-49	4.756844636961581E-4	2.6426914649786564E-5	0.0	1.637979810365746	0.0
50-51	4.756844636961581E-4	2.6426914649786564E-5	0.0	1.9791380650371655	0.0
52-53	4.756844636961581E-4	2.6426914649786564E-5	0.0	2.3223311921366188	0.0
54-55	4.888979210210514E-4	2.6426914649786564E-5	0.0	2.707741315389106	2.6426914649786564E-5
56-57	5.021113783459447E-4	2.6426914649786564E-5	0.0	3.1624824492253083	2.6426914649786564E-5
58-59	5.021113783459447E-4	2.6426914649786564E-5	0.0	3.6464517506641743	2.6426914649786564E-5
60-61	5.021113783459447E-4	2.6426914649786564E-5	0.0	4.165397073642033	2.6426914649786564E-5
62-63	5.285382929957312E-4	2.6426914649786564E-5	0.0	4.691741932721833	2.6426914649786564E-5
64-65	5.285382929957312E-4	2.6426914649786564E-5	0.0	5.2532345882858476	2.6426914649786564E-5
66-67	5.681786649704111E-4	2.6426914649786564E-5	0.0	5.893743718652724	2.6426914649786564E-5
68-69	6.342459515948775E-4	2.6426914649786564E-5	0.0	6.587622003154845	5.285382929957313E-5
70-71	6.342459515948775E-4	2.6426914649786564E-5	0.0	7.331645358204936	5.285382929957313E-5
72-73	6.606728662446641E-4	2.6426914649786564E-5	0.0	8.073620627369667	5.285382929957313E-5
74-75	6.606728662446641E-4	2.6426914649786564E-5	0.0	8.849752683719249	5.285382929957313E-5
76-77	6.606728662446641E-4	2.6426914649786564E-5	0.0	9.700501133582504	5.285382929957313E-5
78-79	6.606728662446641E-4	2.6426914649786564E-5	0.0	10.60979841285236	6.60672866244664E-5
80-81	6.606728662446641E-4	2.6426914649786564E-5	0.0	11.562845449324938	7.928074394935969E-5
82-83	6.606728662446641E-4	2.6426914649786564E-5	0.0	12.521032520696899	7.928074394935969E-5
84-85	6.738863235695573E-4	2.6426914649786564E-5	0.0	13.488799348629408	7.928074394935969E-5
86-87	7.003132382193439E-4	2.6426914649786564E-5	0.0	14.49030013311237	7.928074394935969E-5
88-89	7.135266955442372E-4	2.6426914649786564E-5	0.0	15.554023088138253	7.928074394935969E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3040	0.0	47.70451	1
GTATCAA	5540	0.0	44.973297	1
TCAACGC	7075	0.0	34.826256	4
ATCAACG	7135	0.0	34.799545	3
CAACGCA	7350	0.0	33.45377	5
TATCAAC	7675	0.0	32.472244	2
AACGCAG	7725	0.0	32.075592	6
ACGCAGA	9105	0.0	26.744843	7
GTACATG	11520	0.0	25.920319	1
TACATGG	11510	0.0	25.282297	2
CGCAGAG	9790	0.0	24.678598	8
ACATGGG	11865	0.0	24.207699	3
GCAGAGT	10740	0.0	21.169514	9
CATGGGG	8390	0.0	20.879988	4
AGAGTAC	9335	0.0	18.582365	10-11
GAGTACA	8800	0.0	17.776505	1
TATTAAG	2120	0.0	17.689787	2
CAGAGTA	10360	0.0	17.08744	10-11
AGTACAT	8855	0.0	16.887033	2
GTATTAA	1970	0.0	16.653902	1
>>END_MODULE