FastQCFastQC Report
Mon 15 Aug 2016
SRR2920890_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920890_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3794107
Sequences flagged as poor quality0
Sequence length98
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA68150.038.8990141
GGTATCA38550.033.6671751
TCAACGC84150.031.1240184
CAACGCA84900.030.7410185
ATCAACG85250.030.7224183
AACGCAG89500.029.2596746
TATCAAC90150.029.1057172
GTACATG140050.028.6559851
TACATGG144650.027.3044052
ACATGGG148850.026.0696493
ACGCAGA108400.023.9014427
CATGGGG86800.023.3569564
CGCAGAG110100.023.238088
CGTATCA2706.184564E-1122.1554892
GAGTACA105550.021.583851
AGTACAT104000.020.6424182
GCAGAGT122800.019.864269
CATGGGA67900.018.1717244
AGAGTAC101200.017.69385710-11
ATGGGGA51100.016.9650275