##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920890_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3794107
Sequences flagged as poor quality	0
Sequence length	98
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.93024788178088	31.0	30.0	33.0	23.0	34.0
2	28.94235033434745	31.0	30.0	34.0	22.0	34.0
3	29.018125740786964	31.0	30.0	34.0	23.0	34.0
4	32.53366866037252	35.0	33.0	37.0	25.0	37.0
5	32.50582890782996	35.0	33.0	37.0	25.0	37.0
6	32.64356013154084	35.0	33.0	37.0	25.0	37.0
7	32.48215060882574	35.0	33.0	37.0	25.0	37.0
8	32.429557205424096	35.0	33.0	37.0	25.0	37.0
9	33.72626022407908	37.0	33.0	39.0	25.0	39.0
10-11	33.53212574131409	37.0	33.0	39.0	23.0	39.0
12-13	33.364298371131866	37.0	33.0	39.0	22.5	39.0
14-15	34.15747315507971	38.0	33.0	40.0	22.0	41.0
16-17	34.05370578636818	38.0	33.0	40.0	20.5	41.0
18-19	33.939308907207945	38.0	33.0	40.0	19.0	41.0
20-21	33.847734789767394	38.0	33.0	40.0	18.0	41.0
22-23	33.62795711876339	38.0	32.0	40.0	18.0	41.0
24-25	33.433743302442444	38.0	32.0	40.0	18.0	41.0
26-27	33.493200771617666	38.0	32.0	40.0	17.5	41.0
28-29	33.34759615371944	38.0	32.0	40.0	16.0	41.0
30-31	33.10973135443993	38.0	32.0	40.0	15.5	41.0
32-33	32.89001338127786	38.0	31.5	40.0	12.5	41.0
34-35	32.563096533650736	37.5	31.0	40.0	9.0	41.0
36-37	32.25737189278004	37.0	30.0	40.0	9.0	41.0
38-39	32.073299197940386	37.0	30.0	40.0	8.0	41.0
40-41	31.88511380938914	37.0	30.0	40.0	7.5	41.0
42-43	31.660273682318394	37.0	30.0	40.0	4.0	41.0
44-45	31.39061984809601	36.5	30.0	40.0	2.0	41.0
46-47	31.14654067478856	36.0	29.5	39.5	2.0	41.0
48-49	30.85897155773414	36.0	29.0	39.0	2.0	40.0
50-51	29.69604033307442	34.5	27.0	38.0	2.0	39.5
52-53	30.159099756543505	35.0	28.5	38.5	2.0	39.5
54-55	30.82244464903072	36.0	29.0	39.0	2.0	40.0
56-57	30.785140745898836	36.0	29.0	39.0	2.0	41.0
58-59	30.554708789182804	35.5	29.0	39.0	2.0	41.0
60-61	30.235893848012193	35.0	28.0	39.0	2.0	40.0
62-63	29.85693036595963	35.0	28.0	39.0	2.0	40.0
64-65	29.438298656310955	34.5	28.0	38.0	2.0	40.0
66-67	29.060886527449014	34.0	26.5	38.0	2.0	40.0
68-69	28.622412467545065	34.0	26.0	37.0	2.0	40.0
70-71	28.28850280184507	34.0	26.0	37.0	2.0	39.0
72-73	27.83189417167202	34.0	26.0	36.0	2.0	39.0
74-75	27.39124713667801	33.0	25.0	36.0	2.0	39.0
76-77	26.977345525574265	33.0	24.5	35.0	2.0	37.5
78-79	26.50891250563044	33.0	23.5	35.0	2.0	37.0
80-81	26.053684042121112	32.0	22.0	35.0	2.0	36.5
82-83	25.58374987842989	32.0	20.0	35.0	2.0	36.0
84-85	25.18056198731348	32.0	18.5	35.0	2.0	35.5
86-87	24.727123273012594	32.0	16.5	34.5	2.0	35.0
88-89	24.198584805331002	31.0	8.5	34.0	2.0	35.0
90-91	23.703753478750073	31.0	2.0	34.0	2.0	35.0
92-93	23.167501997176146	31.0	2.0	34.0	2.0	35.0
94-95	22.535497681008998	30.5	2.0	34.0	2.0	35.0
96-97	21.888504594098162	30.0	2.0	34.0	2.0	35.0
98	21.492540932556725	30.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	164863.0
3	29116.0
4	21891.0
5	18110.0
6	16723.0
7	17053.0
8	19564.0
9	22539.0
10	25133.0
11	27172.0
12	27630.0
13	28340.0
14	26434.0
15	27444.0
16	29696.0
17	31535.0
18	32998.0
19	33327.0
20	34780.0
21	37077.0
22	40583.0
23	45783.0
24	52315.0
25	59614.0
26	69294.0
27	80628.0
28	93965.0
29	108201.0
30	126625.0
31	149166.0
32	178241.0
33	216611.0
34	271728.0
35	345754.0
36	441514.0
37	498525.0
38	310791.0
39	33342.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.23479979704316	17.58550138964063	11.965088883562771	25.21460992975344
2	16.509648596934404	19.842407216714992	38.011580741717935	25.636363444632675
3	19.4563627678723	23.71271562382362	28.519549155219625	28.311372453084456
4	13.168674921115272	14.990576010080375	35.70055173177348	36.14019733703088
5	14.603705450458799	36.885969571955854	33.66266159840159	14.84766337918375
6	34.56147626013607	34.05207287344582	17.015359607472305	14.371091258945798
7	30.289546384360698	31.192665387292013	20.521072776735064	17.99671545161222
8	27.51968491483928	33.51580803043307	20.070731789608644	18.893775265119007
9	27.544188922452634	15.244140452549177	19.145690935969913	38.065979689028275
10-11	26.219562658502426	25.118960830298363	27.135114306242365	21.526362204956847
12-13	26.543704416153137	22.309224422405276	27.372798160416128	23.774273001025463
14-15	23.462814774985635	23.990018397566672	24.695662602333172	27.851504225114525
16-17	24.17873632764777	27.2873620903566	25.199604415428116	23.334297166567517
18-19	23.960692668976055	25.989996515746967	26.87060422853589	23.178706586741093
20-21	24.656761911996686	25.518544939286524	26.35275101521032	23.47194213350647
22-23	24.279160864324993	25.333455744770045	26.232203678912164	24.1551797119928
24-25	24.147149490272323	25.506704611133607	26.367681462696694	23.97846443589737
26-27	23.814258283351005	25.915731641055668	26.2865767776037	23.983433297989627
28-29	23.840126204079244	25.92671560977739	26.466780249283122	23.766377936860252
30-31	23.820928331750896	25.772118772310087	26.267511025747552	24.13944187019146
32-33	23.72140633901228	25.845956903194445	26.222034409009197	24.210602348784075
34-35	24.34967625776104	25.783970789490002	25.878370543891986	23.98798240885697
36-37	24.279121794849722	25.490511857231667	26.0426017491102	24.18776459880841
38-39	24.057430902006384	25.690768127326752	26.256138815263263	23.9956621554036
40-41	23.98785589921858	25.462006856078872	26.482223590572435	24.06791365413011
42-43	24.087350827300348	25.31803593761434	26.15234310127831	24.442270133807
44-45	23.94123308648672	25.56633272365858	26.134315868852674	24.358118321002028
46-47	23.88997828470036	25.603296111011296	26.03756241461855	24.469163189669793
48-49	24.10925376009076	25.573802760856957	26.01899729502055	24.297946184031733
50-51	23.725699379674023	25.725597524251842	26.160057197772147	24.38864589830199
52-53	24.1075550491938	25.387311155535414	26.24141279165802	24.26372100361277
54-55	23.54237413986458	25.286409592353813	26.51894002012613	24.652276247655468
56-57	23.542921517861124	25.69634441428604	26.44308576523225	24.317648302620587
58-59	23.693508127161543	25.151183237298515	26.504856822273815	24.650451813266127
60-61	23.570093660750768	25.31179672399083	26.715675896541285	24.402433718717116
62-63	23.383397939867173	25.337297437316053	26.638000866006696	24.64130375681008
64-65	23.247056550106645	25.041285396345756	26.752817710928767	24.958840342618824
66-67	23.285851793044714	25.48363537004743	26.37153633435943	24.858976502548423
68-69	23.401124655615398	24.939048407611118	26.700574897192357	24.959252039581127
70-71	23.01200489829582	25.03626175225304	26.803475785005602	25.148257564445537
72-73	23.2263663848086	24.954581892900283	26.7188991428415	25.10015257944962
74-75	23.092472889102318	24.970584879299174	26.73766049073637	25.19928174086214
76-77	22.98859495462825	24.84072323491252	26.932902063845038	25.23777974661419
78-79	23.00651097359185	24.883104588752257	26.843007462928302	25.267376974727583
80-81	22.87985585368292	25.08829305731207	26.902776874174528	25.12907421483048
82-83	23.027047225936396	24.810832853781967	26.770774270940876	25.391345649340757
84-85	22.857944543373858	24.541221170251465	26.93148720332911	25.66934708304557
86-87	22.90999654131855	24.464895969842836	27.11883902534991	25.506268463488702
88-89	22.738300674964748	24.372971623863524	27.131490423373027	25.7572372777987
90-91	22.67404302700544	24.46145494674355	27.271414487843487	25.593087538407524
92-93	22.865628870974056	24.09031118003421	27.240457635742597	25.803602313249137
94-95	22.68745180014874	23.950380468071465	27.442522503842763	25.919645227937032
96-97	22.912843951775212	23.74294059494447	27.45949222533182	25.8847232279485
98	22.786263640070853	23.776181980799308	27.266892372561763	26.170662006568072
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	51.0
1	51.5
2	114.5
3	207.5
4	213.0
5	241.5
6	384.0
7	657.5
8	1071.0
9	1304.5
10	1232.5
11	1251.5
12	1432.0
13	1606.0
14	1764.5
15	1912.0
16	2065.5
17	2160.0
18	2188.5
19	2189.0
20	2211.0
21	2278.0
22	2463.5
23	2818.0
24	3385.0
25	4309.0
26	5674.0
27	7695.5
28	10654.0
29	14956.0
30	20657.5
31	27927.0
32	34534.5
33	40396.5
34	47355.0
35	56525.5
36	68125.0
37	80067.5
38	87548.0
39	93563.0
40	103534.5
41	114050.0
42	125961.0
43	135599.5
44	142665.0
45	150866.0
46	158327.5
47	170746.0
48	180748.0
49	180075.5
50	178239.0
51	177045.0
52	176112.5
53	174372.5
54	166641.5
55	155462.5
56	140195.0
57	123553.5
58	110313.5
59	95600.5
60	80183.0
61	65993.5
62	54264.0
63	42756.5
64	31746.5
65	24534.0
66	19194.5
67	14855.5
68	10472.0
69	7312.0
70	5701.5
71	4945.5
72	4306.5
73	3314.0
74	2769.0
75	1997.5
76	1173.5
77	670.0
78	330.5
79	178.0
80	100.5
81	54.0
82	35.0
83	26.0
84	24.0
85	19.5
86	12.5
87	8.0
88	6.5
89	7.0
90	6.5
91	4.5
92	4.5
93	7.0
94	4.5
95	1.5
96	1.5
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3688087868897741
2	0.024063633418878277
3	0.018317880861030013
4	0.015734927876309236
5	0.021876030380798432
6	0.022297736990548763
7	0.015471361245215278
8	0.008934908794085143
9	0.0
10-11	2.63566631093957E-5
12-13	0.005416294268980816
14-15	0.0033472962148932543
16-17	0.00600931918894222
18-19	0.035014826940832196
20-21	0.07636843135947405
22-23	0.09518708881958257
24-25	0.06392808637183928
26-27	0.07292888682369791
28-29	0.05675907400608365
30-31	0.05167223802597027
32-33	0.01950393070095282
34-35	0.32464819784998156
36-37	0.49592697306638955
38-39	0.45112064578041683
40-41	0.46014780289538487
42-43	0.4122841026887223
44-45	0.38927473579421984
46-47	0.38860264088493024
48-49	0.37982587206950147
50-51	0.3753057043462401
52-53	0.4321174916785425
54-55	0.4330795098820355
56-57	0.4047197403763257
58-59	0.39101427555943996
60-61	0.4049174153496462
62-63	0.3871925594085776
64-65	0.43329036318691067
66-67	0.45242530060433195
68-69	0.39755072801057006
70-71	0.35867464992421144
72-73	0.34595755997392796
74-75	0.32849627066395337
76-77	0.3269148708773896
78-79	0.3272047941715929
80-81	0.34129243060356496
82-83	0.337233504484718
84-85	0.3371412561638351
86-87	0.3246877328446457
88-89	0.36465761245004424
90-91	0.34552267503262296
92-93	0.3362846646127798
94-95	0.34197770384440923
96-97	0.3421358438230656
98	0.33696993785362406
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3794107.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.2146169631403
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.32090271216353	31.976410118468458
2	12.130172380274322	10.72661850981386
3	5.406669836849602	7.171615076474082
4	2.94182718150898	5.2028704800869665
5	1.8121823511397468	4.006247426150346
6	1.1895580079127903	3.1557511005180143
7	0.8193870662486805	2.5360219695115678
8	0.5723403417251282	2.0244647185543494
9	0.4460098801170158	1.7748140410034985
>10	2.1225651300051243	16.26639949352522
>50	0.13781589525612456	4.2448119165625435
>100	0.09279848124650347	7.94224703302866
>500	0.0060840642795287215	1.8676907476922369
>1k	0.0016866712728467223	1.1040373686101577
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.63566631093957E-5	0.0	0.0	0.0	0.0
3	2.63566631093957E-5	0.0	0.0	0.0	0.0
4	2.63566631093957E-5	0.0	0.0	2.63566631093957E-5	0.0
5	2.63566631093957E-5	0.0	0.0	2.63566631093957E-5	0.0
6	2.63566631093957E-5	0.0	0.0	2.63566631093957E-5	0.0
7	2.63566631093957E-5	0.0	0.0	2.63566631093957E-5	0.0
8	2.63566631093957E-5	0.0	0.0	2.63566631093957E-5	0.0
9	2.63566631093957E-5	0.0	0.0	2.63566631093957E-5	0.0
10-11	2.63566631093957E-5	0.0	0.0	7.90699893281871E-5	0.0
12-13	2.63566631093957E-5	0.0	0.0	1.317833155469785E-4	0.0
14-15	1.054266524375828E-4	0.0	0.0	1.7131831021107206E-4	0.0
16-17	1.054266524375828E-4	0.0	0.0	3.821716150862377E-4	0.0
18-19	1.317833155469785E-4	0.0	0.0	4.217066097503312E-4	0.0
20-21	1.581399786563742E-4	0.0	0.0	4.4806327285972696E-4	0.0
22-23	2.108533048751656E-4	0.0	0.0	6.325599146254969E-4	0.0
24-25	2.63566631093957E-4	0.0	0.0	0.0010938015190399217	0.0
26-27	2.63566631093957E-4	0.0	0.0	0.0020031063963140733	0.0
28-29	3.821716150862377E-4	0.0	0.0	0.007801572280381128	0.0
30-31	4.4806327285972696E-4	0.0	0.0	0.032102415667243964	0.0
32-33	4.4806327285972696E-4	0.0	0.0	0.0778839394882643	0.0
34-35	4.4806327285972696E-4	0.0	0.0	0.14479032879146528	0.0
36-37	4.7441993596912264E-4	0.0	0.0	0.2525495459142296	0.0
38-39	5.27133262187914E-4	0.0	0.0	0.4325919116145117	0.0
40-41	5.27133262187914E-4	0.0	0.0	0.6471219709934379	0.0
42-43	5.534899252973098E-4	0.0	0.0	0.8679117378608457	0.0
44-45	5.534899252973098E-4	0.0	0.0	1.1331783737253587	0.0
46-47	5.666682568520076E-4	0.0	0.0	1.4540312120875876	0.0
48-49	5.798465884067055E-4	0.0	0.0	1.802624438372455	0.0
50-51	5.798465884067055E-4	0.0	0.0	2.1905681626796505	0.0
52-53	5.798465884067055E-4	0.0	0.0	2.576060717317672	5.27133262187914E-5
54-55	5.798465884067055E-4	0.0	0.0	3.0023797431121473	5.27133262187914E-5
56-57	5.798465884067055E-4	0.0	0.0	3.501298197441453	5.27133262187914E-5
58-59	5.798465884067055E-4	0.0	0.0	4.043599719248824	5.27133262187914E-5
60-61	5.798465884067055E-4	0.0	0.0	4.628401360320097	5.27133262187914E-5
62-63	5.798465884067055E-4	0.0	0.0	5.209737100192482	5.27133262187914E-5
64-65	5.798465884067055E-4	0.0	0.0	5.817482216500483	6.589165777348925E-5
66-67	5.798465884067055E-4	0.0	0.0	6.51349579756185	7.90699893281871E-5
68-69	5.798465884067055E-4	0.0	0.0	7.276033596311333	7.90699893281871E-5
70-71	5.930249199614033E-4	0.0	0.0	8.100351413389237	7.90699893281871E-5
72-73	6.325599146254969E-4	0.0	0.0	8.91916068787728	7.90699893281871E-5
74-75	6.589165777348925E-4	0.0	0.0	9.739300973852345	7.90699893281871E-5
76-77	6.589165777348925E-4	0.0	0.0	10.625425165921783	7.90699893281871E-5
78-79	6.589165777348925E-4	0.0	0.0	11.58537437136064	7.90699893281871E-5
80-81	6.984515723989862E-4	0.0	0.0	12.607736155042543	7.90699893281871E-5
82-83	7.11629903953684E-4	2.63566631093957E-5	0.0	13.636489429528478	7.90699893281871E-5
84-85	7.11629903953684E-4	2.63566631093957E-5	0.0	14.641548063879062	7.90699893281871E-5
86	7.379865670630797E-4	2.63566631093957E-5	0.0	15.407762617132306	7.90699893281871E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6815	0.0	38.899014	1
GGTATCA	3855	0.0	33.667175	1
TCAACGC	8415	0.0	31.124018	4
CAACGCA	8490	0.0	30.741018	5
ATCAACG	8525	0.0	30.722418	3
AACGCAG	8950	0.0	29.259674	6
TATCAAC	9015	0.0	29.105717	2
GTACATG	14005	0.0	28.655985	1
TACATGG	14465	0.0	27.304405	2
ACATGGG	14885	0.0	26.069649	3
ACGCAGA	10840	0.0	23.901442	7
CATGGGG	8680	0.0	23.356956	4
CGCAGAG	11010	0.0	23.23808	8
CGTATCA	270	6.184564E-11	22.15548	92
GAGTACA	10555	0.0	21.58385	1
AGTACAT	10400	0.0	20.642418	2
GCAGAGT	12280	0.0	19.86426	9
CATGGGA	6790	0.0	18.171724	4
AGAGTAC	10120	0.0	17.693857	10-11
ATGGGGA	5110	0.0	16.965027	5
>>END_MODULE