FastQCFastQC Report
Mon 15 Aug 2016
SRR2920890_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920890_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3794107
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA68700.040.2878721
GGTATCA37900.039.4002081
TCAACGC84550.031.6676714
ATCAACG86950.030.9027753
CAACGCA86800.030.7329445
AACGCAG90450.029.6501886
TATCAAC92250.029.484842
GTACATG145450.028.1839351
TACATGG150200.026.6736852
ACATGGG156850.025.1819553
ACGCAGA111350.023.615427
CGCAGAG114600.022.7770188
AGTACAT108050.021.5708482
GAGTACA114700.021.3526551
CATGGGG95350.020.9112134
GCAGAGT128000.019.1319189
AGAGTAC108650.017.86349910-11
CATGGGA66950.017.7981054
GTATATA11150.017.487071
GCACCGT10350.017.4273286
Produced by FastQC (version 0.11.5)