##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920890_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3794107
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.321654871620648	31.0	30.0	34.0	27.0	34.0
2	30.324966849906975	31.0	30.0	34.0	26.0	34.0
3	30.463311393168407	31.0	30.0	34.0	27.0	34.0
4	34.117083413830976	35.0	35.0	37.0	30.0	37.0
5	33.66524507611409	35.0	35.0	37.0	28.0	37.0
6	33.61719108079978	35.0	35.0	37.0	28.0	37.0
7	33.42706913642657	35.0	35.0	37.0	28.0	37.0
8	33.36292729751691	35.0	35.0	37.0	27.0	37.0
9	34.696895211442374	38.0	35.0	39.0	27.0	39.0
10-11	34.50795509984299	37.5	34.0	39.0	27.0	39.0
12-13	34.32383983899241	37.0	34.0	39.0	27.0	39.0
14-15	35.241507290121234	38.0	34.0	40.0	27.0	41.0
16-17	35.12051926843392	38.0	34.0	40.0	26.0	41.0
18-19	35.015645578788366	38.5	34.0	40.0	25.5	41.0
20-21	34.902436726217786	38.0	34.0	40.0	25.0	41.0
22-23	34.75884536203117	38.0	34.0	40.0	25.0	41.0
24-25	34.57880233741431	38.0	33.5	40.0	24.0	41.0
26-27	34.40689614183258	38.0	33.0	40.0	24.0	41.0
28-29	34.35150656004166	38.0	33.0	40.0	23.5	41.0
30-31	34.341651144788486	38.0	33.0	40.0	24.0	41.0
32-33	34.20916911410248	38.0	33.0	40.0	23.0	41.0
34-35	34.038423929530715	38.0	33.0	40.0	22.5	41.0
36-37	33.84698494270194	38.0	33.0	40.0	21.5	41.0
38-39	33.67550308939627	38.0	33.0	40.0	19.5	41.0
40-41	33.44959762600264	38.0	32.0	40.0	18.5	41.0
42-43	33.48028890592701	38.0	32.0	40.0	18.5	41.0
44-45	33.34233747229585	38.0	32.0	40.0	17.5	41.0
46-47	33.10513923302638	38.0	32.0	40.0	15.0	41.0
48-49	32.84693394255881	37.0	31.5	40.0	14.5	41.0
50-51	32.592582128021164	37.0	31.0	40.0	12.0	41.0
52-53	32.332867918590594	37.0	31.0	40.0	9.0	41.0
54-55	32.05281374510524	36.5	31.0	40.0	8.0	41.0
56-57	31.738821941500333	36.0	30.0	39.0	7.5	41.0
58-59	31.413266942656072	36.0	30.0	39.0	6.5	40.5
60-61	31.08470425320108	35.0	29.0	39.0	2.0	40.0
62-63	30.7767453579986	35.0	29.0	39.0	2.0	40.0
64-65	30.376066621210207	35.0	29.0	38.0	2.0	40.0
66-67	29.947265720234036	34.5	28.0	38.0	2.0	40.0
68-69	29.512571732953234	34.0	28.0	37.0	2.0	39.5
70-71	29.092461414504125	34.0	27.5	37.0	2.0	39.0
72-73	28.651854441638044	34.0	26.5	36.0	2.0	39.0
74-75	28.207492566762085	33.5	26.0	36.0	2.0	38.5
76-77	26.757786878440697	31.5	25.0	34.5	2.0	36.0
78-79	27.609008127604202	33.0	26.0	35.0	2.0	37.0
80-81	27.64122756685565	33.0	26.0	35.0	2.0	37.0
82-83	27.303586193009316	33.0	26.0	35.0	2.0	36.0
84-85	26.98551806788791	33.0	25.5	35.0	2.0	36.0
86-87	26.446079538610796	33.0	24.5	35.0	2.0	35.0
88-89	26.08714079492223	32.0	23.5	35.0	2.0	35.0
90-91	25.731932968680113	32.0	22.5	35.0	2.0	35.0
92-93	25.43093684495456	32.0	20.0	34.0	2.0	35.0
94-95	25.053613669830607	32.0	19.0	34.0	2.0	35.0
96-97	24.52942062519586	32.0	14.0	34.0	2.0	35.0
98-99	23.927075725592346	32.0	2.0	34.0	2.0	35.0
100-101	21.716946965386057	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	82392.0
3	38127.0
4	20505.0
5	14857.0
6	12800.0
7	12616.0
8	14072.0
9	16170.0
10	18413.0
11	20931.0
12	23547.0
13	25229.0
14	27378.0
15	29330.0
16	30907.0
17	32369.0
18	31289.0
19	30045.0
20	30667.0
21	32294.0
22	35023.0
23	39718.0
24	46474.0
25	54633.0
26	66003.0
27	77649.0
28	91707.0
29	108406.0
30	127825.0
31	151313.0
32	181644.0
33	221973.0
34	280601.0
35	363431.0
36	482273.0
37	567285.0
38	330166.0
39	24043.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.26658429652617	18.91477383903917	12.653622116461943	25.16501974797272
2	16.603248142448276	20.91145558098388	36.953412225854464	25.531884050713384
3	19.292646711107444	24.514565474055022	28.873097056855073	27.319690757982457
4	13.586558396455079	15.923075605065906	35.309594197588304	35.18077180089072
5	14.542047107943404	37.22328827783986	33.68086160723699	14.553803006979734
6	34.505013362899085	34.580027129495186	16.74743685373846	14.167522653867273
7	30.073528266691934	31.420169555544238	20.531851910898215	17.974450266865613
8	27.410040521514933	33.96774376574316	19.920619526593647	18.701596186148265
9	27.333202591020537	15.282861345665694	19.129720745505686	38.25421531780808
10-11	26.244705073972618	25.317009493858773	27.10368832679414	21.33459710537447
12-13	26.510641211687343	22.433417635877657	27.42504829178171	23.63089286065329
14-15	23.41562259746658	24.09503117661285	24.657594251876738	27.831751974043833
16-17	24.140780835715603	27.425727663572157	25.224294635997442	23.2091968647148
18-19	23.96933696185176	26.099376984758166	26.858936466372768	23.07234958701731
20-21	24.68823225573063	25.524205126393255	26.3433296059454	23.444233011930717
22-23	24.25289562176318	25.46788618127453	26.239480433490886	24.039737763471408
24-25	24.122225036719964	25.66676455141118	26.394847719775537	23.816162692093318
26-27	23.803792369553804	26.01537715138818	26.316970081638452	23.863860397419568
28-29	23.81700026446649	25.94915915213573	26.521704050134197	23.712136533263582
30-31	23.836534129360366	25.87086108166492	26.25217792712219	24.040426861852524
32-33	23.775573036525085	25.88715744591883	26.26068562821482	24.076583889341272
34-35	24.288649499070477	25.87569081455221	25.934082504426172	23.901577181951144
36-37	24.263197473899	25.584338806508228	26.104902262537085	24.047561457055693
38-39	24.01520328701477	25.73499867118884	26.36949281598286	23.88030522581353
40-41	23.945077043779765	25.502917350352	26.525136772217838	24.026868833650393
42-43	24.067843343380023	25.43545456031202	26.217530370711685	24.279171725596278
44-45	23.863296480740146	25.639397171352236	26.171932104333557	24.325374243574064
46-47	23.82230750713791	25.671361643460717	26.114822991175068	24.39150785822631
48-49	23.98702720028341	25.69234305379537	26.06769389084882	24.2529358550724
50-51	23.71143450672138	25.72807836921306	26.175550717201297	24.384936406864266
52-53	24.075269483253752	25.452635657586587	26.189910736761085	24.282184122398576
54-55	23.53847181882416	25.376851074844865	26.428426731526088	24.656250374804888
56-57	23.495141613380518	25.75037820545515	26.30375284980232	24.450727331362014
58-59	23.61566786861831	25.229848376442764	26.426550457654034	24.72793329728489
60-61	23.525300717266123	25.477216271916557	26.461129026836684	24.53635398398064
62-63	23.27404475671387	25.575603973132	26.35649719636268	24.793854073791444
64-65	23.08873575749301	25.233187403676016	26.461561210985078	25.216515627845897
66-67	23.189822958856162	25.70830893783092	26.081897959320894	25.01997014399202
68-69	23.30583598912945	25.188779057504885	26.381001889274508	25.12438306409116
70-71	22.924832987238197	25.261416723763357	26.398469079341247	25.415281209657202
72-73	23.171577749129906	25.226498513214164	26.25746590096163	25.3444578366943
74-75	23.08889950235842	25.257608558448585	26.143204545871225	25.510287393321775
76-77	22.960055322951696	25.165694750197982	26.41125375573226	25.462996171118064
78-79	22.9453847496074	25.256798467656704	26.268468961093035	25.529347821642855
80-81	22.829960754851275	25.490841151059225	26.26290624989703	25.41629184419247
82-83	22.943240594444102	25.293489509608396	26.05101614218663	25.712253753760876
84-85	22.712249845595498	24.983491964244056	26.168591000596532	26.135667189563915
86-87	22.763918382274444	24.976944070675604	26.298240403184124	25.96089714386583
88-89	22.538852339627187	25.005052302603488	26.249669147112975	26.206426210656353
90-91	22.44167037525251	25.101387099195243	26.29448585258186	26.16245667297038
92-93	22.550461909005236	24.771425994446037	26.27426024069999	26.403851855848735
94-95	22.308856376883657	24.652722821500973	26.460605503114547	26.577815298500823
96-97	22.520806179319944	24.501304051587464	26.446548381697678	26.531341387394914
98-99	22.304206010846144	24.50543295620029	26.392978340658544	26.79738269229502
100-101	22.295845829197333	24.39009025488663	26.632743108582623	26.681320807333414
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	58.0
1	50.0
2	93.0
3	156.5
4	143.5
5	155.0
6	276.5
7	521.0
8	770.0
9	800.5
10	661.0
11	610.5
12	623.5
13	646.5
14	732.0
15	790.5
16	855.5
17	966.0
18	1048.5
19	1162.0
20	1314.5
21	1505.0
22	1803.5
23	2235.5
24	2748.5
25	3427.0
26	4554.0
27	6253.0
28	8541.5
29	11943.5
30	16696.0
31	22658.5
32	30192.0
33	36358.5
34	41329.0
35	47969.0
36	57681.0
37	69167.0
38	80009.5
39	87314.5
40	92848.0
41	103198.0
42	115736.5
43	126545.0
44	134118.0
45	141714.5
46	149764.5
47	157118.5
48	170476.5
49	179458.5
50	178540.5
51	176625.0
52	173724.5
53	171528.0
54	167543.0
55	156418.5
56	140352.0
57	124182.0
58	110549.0
59	97086.0
60	82190.5
61	66883.5
62	54035.5
63	42892.5
64	33052.5
65	25186.5
66	18786.0
67	15165.0
68	11113.5
69	7245.5
70	5616.0
71	4683.5
72	4045.0
73	3460.0
74	2684.0
75	2074.5
76	1328.0
77	668.5
78	329.5
79	160.0
80	77.0
81	42.0
82	23.0
83	17.0
84	11.5
85	6.0
86	3.5
87	2.5
88	2.0
89	2.0
90	1.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18808114794864772
2	0.0
3	0.011702358420571692
4	0.013494611512010599
5	0.007037229050208652
6	0.10901115862046062
7	0.1448562204492388
8	0.12551042972694235
9	0.14453994049192603
10-11	0.11581117770268473
12-13	0.11553443274003607
14-15	0.11827552570341322
16-17	0.06731491758139661
18-19	0.0798079758952502
20-21	0.03848072813971772
22-23	0.08577776008952832
24-25	0.0588412503917259
26-27	0.06780251584892044
28-29	0.04127453442931367
30-31	0.02466983667039438
32-33	0.03216830732501746
34-35	0.02000470730003134
36-37	0.02397138509799539
38-39	0.03276133224497886
40-41	0.024748906659722566
42-43	0.012782981608056916
44-45	0.026949688029357103
46-47	0.035054361935496284
48-49	0.008513202184334812
50-51	0.015194616282566622
52-53	0.00716901236575563
54-55	0.012295383340533095
56-57	0.005363580942762025
58-59	0.0036503978406513047
60-61	0.0233124685202605
62-63	0.009870570334468691
64-65	0.0071162990395368395
66-67	0.04139313941330595
68-69	0.09220878588822087
70-71	0.021981457033236014
72-73	0.044753613959753906
74-75	0.0876754398334048
76-77	0.23877818943957035
78-79	0.08040100081521159
80-81	0.01698686937400553
82-83	0.011728715083681087
84-85	0.01317833155469785
86-87	0.25911235502846913
88-89	0.6206730595631594
90-91	0.2660705140893496
92-93	0.3527575790561521
94-95	0.0014627948025714614
96-97	0.004269779423722104
98-99	0.003479079530440233
100-101	0.004875982675238205
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3794107.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.86374256759247
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.30218000562688	29.4311236569611
2	12.73317600425502	10.661168046199556
3	5.858867445286696	7.358223554013911
4	3.2459009253467417	5.435422429545047
5	1.9939911883545147	4.173796689566059
6	1.2553251937463	3.1531566449764945
7	0.8993714209226509	2.635573754670899
8	0.6281474897186601	2.103728384324914
9	0.47330561476755606	1.783290997118255
>10	2.3415887413023313	17.052358415504802
>50	0.15479492642459072	4.504016846037504
>100	0.10472826035749032	8.56881811063402
>500	0.006974310505871632	2.092209231928787
>1k	0.0016484733846138723	1.0471132385186415
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.63566631093957E-5
2	2.63566631093957E-5	0.0	0.0	0.0	2.63566631093957E-5
3	2.63566631093957E-5	0.0	0.0	0.0	2.63566631093957E-5
4	2.63566631093957E-5	0.0	0.0	5.27133262187914E-5	2.63566631093957E-5
5	2.63566631093957E-5	0.0	0.0	5.27133262187914E-5	2.63566631093957E-5
6	5.27133262187914E-5	0.0	0.0	5.27133262187914E-5	2.63566631093957E-5
7	7.90699893281871E-5	0.0	0.0	5.27133262187914E-5	2.63566631093957E-5
8	7.90699893281871E-5	0.0	0.0	5.27133262187914E-5	2.63566631093957E-5
9	7.90699893281871E-5	0.0	0.0	1.054266524375828E-4	2.63566631093957E-5
10-11	7.90699893281871E-5	0.0	0.0	1.581399786563742E-4	2.63566631093957E-5
12-13	1.054266524375828E-4	0.0	0.0	2.2403163642986348E-4	2.63566631093957E-5
14-15	1.054266524375828E-4	0.0	0.0	2.63566631093957E-4	2.63566631093957E-5
16-17	1.054266524375828E-4	0.0	0.0	4.612416044144248E-4	2.63566631093957E-5
18-19	1.317833155469785E-4	0.0	0.0	5.007765990785183E-4	2.63566631093957E-5
20-21	1.7131831021107206E-4	0.0	0.0	5.798465884067055E-4	2.63566631093957E-5
22-23	1.8449664176576992E-4	0.0	0.0	7.511648986177775E-4	2.63566631093957E-5
24-25	2.63566631093957E-4	0.0	0.0	0.001133336513704015	2.63566631093957E-5
26-27	2.63566631093957E-4	0.0	0.0	0.002029463059423469	2.63566631093957E-5
28-29	3.689932835315398E-4	0.0	0.0	0.00809149557458448	2.63566631093957E-5
30-31	3.9534994664093553E-4	0.0	0.0	0.032669083924095975	2.63566631093957E-5
32-33	3.9534994664093553E-4	0.0	0.0	0.07837153775578812	2.63566631093957E-5
34-35	4.348849413050291E-4	0.0	0.0	0.14500118209634044	2.63566631093957E-5
36-37	5.534899252973098E-4	0.0	0.0	0.25318210582885514	2.63566631093957E-5
38-39	6.062032515161012E-4	0.0	0.0	0.43321129319758245	2.63566631093957E-5
40-41	6.062032515161012E-4	0.0	0.0	0.6492963956999631	2.63566631093957E-5
42-43	7.379865670630797E-4	0.0	0.0	0.8717729890063723	2.63566631093957E-5
44-45	7.906998932818711E-4	0.0	0.0	1.1367101665820178	2.63566631093957E-5
46-47	8.565915510553603E-4	0.0	0.0	1.4570490500136133	2.63566631093957E-5
48-49	8.961265457194539E-4	0.0	0.0	1.8030066099875413	2.63566631093957E-5
50-51	8.961265457194539E-4	1.317833155469785E-5	0.0	2.1855867533519744	2.63566631093957E-5
52-53	8.961265457194539E-4	2.63566631093957E-5	0.0	2.5657552620418977	2.63566631093957E-5
54-55	8.961265457194539E-4	2.63566631093957E-5	0.0	2.987119235171807	2.63566631093957E-5
56-57	8.961265457194539E-4	2.63566631093957E-5	0.0	3.480357828601038	2.63566631093957E-5
58-59	8.961265457194539E-4	2.63566631093957E-5	0.0	4.017427552781195	2.63566631093957E-5
60-61	9.093048772741518E-4	2.63566631093957E-5	0.0	4.595824524716884	2.63566631093957E-5
62-63	9.224832088288496E-4	2.63566631093957E-5	0.0	5.172323816908696	5.27133262187914E-5
64-65	9.224832088288496E-4	2.63566631093957E-5	0.0	5.774428607311286	5.27133262187914E-5
66-67	0.0010015531981570366	2.63566631093957E-5	0.0	6.462100304498529	5.27133262187914E-5
68-69	0.0010279098612664323	2.63566631093957E-5	0.0	7.214912494560643	5.27133262187914E-5
70-71	0.00104108819282113	2.63566631093957E-5	0.0	8.028924856362776	5.27133262187914E-5
72-73	0.001067444855930526	2.63566631093957E-5	0.0	8.836611619018651	5.27133262187914E-5
74-75	0.0010806231874852237	2.63566631093957E-5	0.0	9.652996080500628	5.27133262187914E-5
76-77	0.0010806231874852237	2.63566631093957E-5	0.0	10.534099328247727	5.27133262187914E-5
78-79	0.0010806231874852237	2.63566631093957E-5	0.0	11.485693471480904	5.27133262187914E-5
80-81	0.001238763166141598	2.63566631093957E-5	0.0	12.500451357855749	5.27133262187914E-5
82-83	0.001238763166141598	2.63566631093957E-5	0.0	13.521508486713739	5.27133262187914E-5
84-85	0.0012782981608056914	2.63566631093957E-5	0.0	14.521836100036188	5.27133262187914E-5
86-87	0.001317833155469785	2.63566631093957E-5	0.0	15.550668444511448	5.27133262187914E-5
88-89	0.001317833155469785	2.63566631093957E-5	0.0	16.651111842654938	5.27133262187914E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6870	0.0	40.287872	1
GGTATCA	3790	0.0	39.400208	1
TCAACGC	8455	0.0	31.667671	4
ATCAACG	8695	0.0	30.902775	3
CAACGCA	8680	0.0	30.732944	5
AACGCAG	9045	0.0	29.650188	6
TATCAAC	9225	0.0	29.48484	2
GTACATG	14545	0.0	28.183935	1
TACATGG	15020	0.0	26.673685	2
ACATGGG	15685	0.0	25.181955	3
ACGCAGA	11135	0.0	23.61542	7
CGCAGAG	11460	0.0	22.777018	8
AGTACAT	10805	0.0	21.570848	2
GAGTACA	11470	0.0	21.352655	1
CATGGGG	9535	0.0	20.911213	4
GCAGAGT	12800	0.0	19.131918	9
AGAGTAC	10865	0.0	17.863499	10-11
CATGGGA	6695	0.0	17.798105	4
GTATATA	1115	0.0	17.48707	1
GCACCGT	1035	0.0	17.427328	6
>>END_MODULE