FastQCFastQC Report
Mon 15 Aug 2016
SRR2920889_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920889_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3552774
Sequences flagged as poor quality0
Sequence length98
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39350.044.443641
GTATCAA76050.042.3613321
TCAACGC93500.034.1441844
ATCAACG95100.033.4732633
CAACGCA96550.032.8280335
AACGCAG100250.031.6577246
TATCAAC103100.031.0996822
GTACATG148500.028.2333721
TACATGG151600.027.1110442
ACGCAGA116900.026.6753167
CGCAGAG120700.025.4914048
ACATGGG159400.025.466783
CATGGGG103900.022.6916834
GCAGAGT131500.022.177059
GTATTAG9700.019.4528351
GAGTACA111300.018.6902051
AGAGTAC116950.018.42778210-11
GGACCGT4500.018.345016
GTATATG10150.017.6835461
CAGAGTA124950.017.63325910-11
Produced by FastQC (version 0.11.5)