##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920889_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3552774
Sequences flagged as poor quality	0
Sequence length	98
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.9046528149553	31.0	30.0	33.0	23.0	34.0
2	28.928045521612127	31.0	30.0	34.0	22.0	34.0
3	29.001981268721288	31.0	30.0	34.0	23.0	34.0
4	32.51938260075085	35.0	33.0	37.0	25.0	37.0
5	32.489790512990695	35.0	33.0	37.0	25.0	37.0
6	32.61632431446526	35.0	33.0	37.0	25.0	37.0
7	32.46251295466585	35.0	33.0	37.0	25.0	37.0
8	32.40471839751135	35.0	33.0	37.0	25.0	37.0
9	33.69334497494071	37.0	33.0	39.0	25.0	39.0
10-11	33.50431423445454	37.0	33.0	39.0	23.0	39.0
12-13	33.349933319710175	37.0	33.0	39.0	22.0	39.0
14-15	34.132440172102136	38.0	33.0	40.0	21.5	41.0
16-17	34.02781305537589	38.0	33.0	40.0	20.0	41.0
18-19	33.919140789704045	38.0	33.0	40.0	19.0	41.0
20-21	33.82660141061604	38.0	33.0	40.0	18.0	41.0
22-23	33.60839009179869	38.0	32.0	40.0	18.0	41.0
24-25	33.40952267158001	38.0	32.0	40.0	17.5	41.0
26-27	33.43916148339298	38.0	32.0	40.0	17.0	41.0
28-29	33.27767147586647	38.0	32.0	40.0	16.0	41.0
30-31	33.029909163937816	38.0	32.0	40.0	14.5	41.0
32-33	32.78177812605024	38.0	31.0	40.0	11.0	41.0
34-35	32.453079903196766	37.0	31.0	40.0	9.0	41.0
36-37	32.14042435572879	37.0	30.0	40.0	8.0	41.0
38-39	31.929970777764076	37.0	30.0	40.0	8.0	41.0
40-41	31.72356628932772	37.0	30.0	40.0	4.5	41.0
42-43	31.47831623964823	37.0	30.0	40.0	2.0	41.0
44-45	31.193456859344277	36.0	30.0	40.0	2.0	41.0
46-47	30.914441363284013	36.0	29.0	39.0	2.0	40.5
48-49	30.601952727643244	36.0	28.5	39.0	2.0	40.0
50-51	29.434010015835515	34.0	27.0	38.0	2.0	39.5
52-53	29.87642445030278	34.5	28.0	38.5	2.0	39.5
54-55	30.52569344405245	36.0	29.0	39.0	2.0	40.0
56-57	30.474457001768194	35.5	28.5	39.0	2.0	41.0
58-59	30.22471806537652	35.0	28.0	39.0	2.0	40.5
60-61	29.897600016212685	35.0	28.0	39.0	2.0	40.0
62-63	29.505283054874866	35.0	28.0	38.5	2.0	40.0
64-65	29.08287552205685	34.0	26.5	38.0	2.0	40.0
66-67	28.698154174737823	34.0	26.0	37.5	2.0	40.0
68-69	28.257071797980956	34.0	26.0	37.0	2.0	39.5
70-71	27.9321041811272	34.0	26.0	36.5	2.0	39.0
72-73	27.480974303459774	33.5	25.0	36.0	2.0	39.0
74-75	27.055685219493277	33.0	24.5	35.5	2.0	38.5
76-77	26.6476221116232	33.0	23.5	35.0	2.0	37.5
78-79	26.20196894032663	32.5	22.5	35.0	2.0	37.0
80-81	25.77328082225326	32.0	20.5	35.0	2.0	36.0
82-83	25.305943609134722	32.0	19.5	35.0	2.0	36.0
84-85	24.921071534524852	32.0	17.5	35.0	2.0	35.5
86-87	24.47296042472727	31.5	12.5	34.0	2.0	35.0
88-89	23.9467292318622	31.0	4.5	34.0	2.0	35.0
90-91	23.452162310352417	31.0	2.0	34.0	2.0	35.0
92-93	22.922418650890826	31.0	2.0	34.0	2.0	35.0
94-95	22.29599785970062	30.0	2.0	34.0	2.0	35.0
96-97	21.658979293363437	30.0	2.0	34.0	2.0	35.0
98	21.266592527416606	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	157170.0
3	27668.0
4	20690.0
5	17259.0
6	16154.0
7	16779.0
8	19182.0
9	22310.0
10	24205.0
11	26446.0
12	27486.0
13	27688.0
14	25827.0
15	27203.0
16	29882.0
17	32033.0
18	32812.0
19	31945.0
20	32982.0
21	35196.0
22	38585.0
23	42859.0
24	49198.0
25	56316.0
26	65788.0
27	76259.0
28	88122.0
29	102759.0
30	119483.0
31	141027.0
32	169241.0
33	205174.0
34	256452.0
35	327451.0
36	413611.0
37	449840.0
38	270190.0
39	29502.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.03481537498033	17.820319387879916	12.632151954302294	24.512713282837456
2	16.199816439374317	19.602585614702868	38.80415319992343	25.393444745999393
3	19.275640453575615	24.059867161430702	28.89772364814248	27.766768736851205
4	13.216020746468615	15.392434982190007	36.110301757858956	35.28124251348242
5	14.455055692630284	37.11704630298007	33.72745915254409	14.700438851845554
6	33.8098604456314	34.62324487204068	17.17721735306447	14.389677329263456
7	29.68041388605972	31.640646772975796	20.70153777205751	17.977401568906973
8	27.41112978974712	33.66928605449032	19.930645711564075	18.98893844419848
9	27.539072285487343	15.236375857287854	19.26548100160607	37.959070855618734
10-11	26.037946420717905	25.023921458522885	27.277344665328272	21.660787455430935
12-13	26.470705796351236	22.564661851597577	27.315242905401316	23.649389446649874
14-15	23.703093311640696	24.12400268530807	24.694264728028116	27.478639275023113
16-17	23.755397485770903	27.136686577079196	25.537581138215042	23.57033479893486
18-19	23.882857977912543	26.137415043370186	27.03080767153651	22.94891930718076
20-21	24.63700839767097	25.4900988718508	26.479479323555534	23.39341340692269
22-23	24.276630352331086	25.20539064968426	26.57223878238064	23.945740215604015
24-25	23.951045479796967	25.646992058479555	26.57455657673224	23.827405884991236
26-27	23.68407488162258	25.7509019667932	26.530864882571798	24.034158269012423
28-29	23.871834222759368	25.846947191059805	26.524186713426833	23.75703187275399
30-31	23.910085369476786	25.737862341542677	26.405428276544722	23.94662401243581
32-33	23.6110061276999	25.829212939718033	26.175186651121145	24.384594281460924
34-35	24.253533257091224	25.775029296737117	26.220452652237142	23.750984793934514
36-37	24.285639733284945	25.42394622818147	26.25879200941001	24.03162202912357
38-39	24.06848460867624	25.527935884174653	26.1956229326066	24.207956574542507
40-41	24.04295209101758	25.549247064581813	26.45097687985734	23.95682396454326
42-43	24.162303901674147	25.455688024337274	26.175629035157232	24.20637903883134
44-45	23.964223098451466	25.525047645553812	26.298424341403525	24.212304914591197
46-47	24.143207446597348	25.512637343153706	26.165770059026492	24.178385151222454
48-49	23.91129121116628	25.66256271341207	25.915252771990733	24.510893303430922
50-51	23.737994343199656	25.9024698461013	26.17177738881723	24.187758421881814
52-53	24.094418543250782	25.439557845753143	26.21877499053734	24.247248620458738
54-55	23.781036964503176	25.447003524467178	26.43453559463087	24.33742391639878
56-57	23.652559868840395	25.679243099545733	26.307798629934325	24.36039840167954
58-59	23.608084178260842	25.235860585618614	26.38024605961864	24.7758091765019
60-61	23.409947891512385	25.29855911087473	26.72978333958349	24.561709658029397
62-63	23.325887764369853	25.315240567788322	26.702995542971564	24.655876124870257
64-65	23.36140078907741	25.262165979891726	26.573342164306418	24.80309106672445
66-67	23.357391612281667	25.390147721272672	26.507680813481304	24.744779852964356
68-69	23.585974656745933	25.069224446013692	26.469862675766798	24.87493822147358
70-71	23.17103742134301	25.057406979790514	26.713583694002686	25.05797190486379
72-73	23.210605588610505	25.011671298941433	26.668783928317747	25.108939184130307
74-75	23.220092859870824	25.163683700388905	26.638852473945374	24.977370965794897
76-77	23.1944385812554	24.912728686139104	26.776441566799104	25.11639116580639
78-79	23.14794551879833	24.886266891247967	26.79476083644768	25.171026753506027
80-81	23.100220342075282	24.87771325480999	26.852192321748003	25.16987408136672
82-83	23.10892937325791	24.767269790257583	26.85681486355588	25.26698597292863
84-85	22.818806161256425	24.848638715673484	26.952517501258814	25.380037621811276
86-87	23.022747850169118	24.6326026588455	26.951239157311758	25.393410333673632
88-89	22.901603802233033	24.59580087711245	26.81941511712488	25.683180203529638
90-91	22.78653704912653	24.57859966230075	27.01177400275	25.62308928582272
92-93	22.930311248972068	24.427506461290992	27.051276330890396	25.590905958846548
94-95	22.702371970540046	24.072697295540973	27.41415997658206	25.810770757336925
96-97	22.96571161243578	23.73442240854526	27.383970040076385	25.91589593894258
98	22.9253709931623	23.758557714926674	27.30109799121912	26.014973300691903
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	252.0
1	180.0
2	198.0
3	293.0
4	256.0
5	278.0
6	459.5
7	830.5
8	1255.0
9	1415.0
10	1321.5
11	1268.5
12	1366.0
13	1557.0
14	1776.5
15	1923.5
16	2032.5
17	2125.0
18	2195.0
19	2260.5
20	2345.0
21	2444.5
22	2669.5
23	3083.5
24	3666.5
25	4656.0
26	6127.5
27	8325.5
28	11762.5
29	16201.5
30	21790.0
31	29808.5
32	36554.0
33	42304.0
34	50047.5
35	58250.0
36	68105.5
37	78095.0
38	85212.0
39	90701.0
40	96895.0
41	103861.5
42	112391.0
43	121241.5
44	129414.5
45	137729.0
46	145198.5
47	151211.5
48	155209.0
49	156323.0
50	155986.0
51	155889.5
52	155330.0
53	155503.5
54	151700.0
55	143076.5
56	132621.5
57	120268.5
58	110080.5
59	97704.5
60	82194.0
61	67540.0
62	54800.5
63	42094.0
64	30704.5
65	23469.5
66	18104.5
67	14079.5
68	10581.5
69	8046.0
70	5996.0
71	4397.5
72	3719.0
73	3123.5
74	2381.0
75	1735.5
76	1186.5
77	729.5
78	395.0
79	191.5
80	100.5
81	63.5
82	54.0
83	48.0
84	30.0
85	21.5
86	19.0
87	16.0
88	11.0
89	5.5
90	3.0
91	5.0
92	4.5
93	3.0
94	3.5
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3607040582936038
2	0.02291167408903578
3	0.01809853370915234
4	0.015621595969797121
5	0.02189838137748137
6	0.023221291306455182
7	0.013848333724576908
8	0.008162635731966064
9	0.0
10-11	4.222052964810033E-5
12-13	0.004982022498475839
14-15	0.003068025154428624
16-17	0.0054605218344876425
18-19	0.03535265682534267
20-21	0.07382963284464478
22-23	0.09168891688579121
24-25	0.05999537262995056
26-27	0.06972016795889635
28-29	0.053155646826958315
30-31	0.048947667372030976
32-33	0.017605960863257837
34-35	0.3215515537999321
36-37	0.48949074723019254
38-39	0.446482101028661
40-41	0.45548914735358903
42-43	0.40807549255877235
44-45	0.38333426218498556
46-47	0.3828135423193257
48-49	0.3732294820892069
50-51	0.36986591322724155
52-53	0.4274124951376024
54-55	0.4283413467898605
56-57	0.3992514018623194
58-59	0.3859800820429332
60-61	0.3995469455698561
62-63	0.3808995449752785
64-65	0.42853837592821836
66-67	0.44687615930537655
68-69	0.39189095619366726
70-71	0.3513170272018429
72-73	0.3388056769161224
74-75	0.32134045115169163
76-77	0.3201864233413102
78-79	0.32000346771283505
80-81	0.33539988752450905
82-83	0.3311215405201682
84-85	0.33053045310509477
86-87	0.31734357434500476
88-89	0.3588463549890874
90-91	0.33915751466318994
92-93	0.328926072978467
94-95	0.3349354616983799
96-97	0.3352450789157993
98	0.328503867681986
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3552774.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.17323325467045
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.6507059939198	27.67616585506964
2	10.655795898273725	8.348439564744746
3	5.188901053906085	6.097980939602054
4	3.111008402155243	4.8747303117946785
5	2.16767991032905	4.24575153743915
6	1.5249686687096553	3.5842772019256555
7	1.1189787241574416	3.0683810197903103
8	0.8579340265408266	2.6886439791041945
9	0.6699456858868152	2.3619544759083957
>10	3.8146381089577663	26.134599518425034
>50	0.16410061825385286	4.341601115578446
>100	0.07098790613464837	4.929539796071674
>500	0.0029759185645003945	0.7960458848450366
>1k	0.0013790842105596678	0.8518887997009172
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.444105929620065E-5
2	0.0	0.0	0.0	0.0	8.444105929620065E-5
3	0.0	0.0	0.0	0.0	8.444105929620065E-5
4	0.0	0.0	0.0	0.0	8.444105929620065E-5
5	0.0	0.0	0.0	0.0	1.1258807906160088E-4
6	0.0	0.0	0.0	0.0	1.688821185924013E-4
7	0.0	0.0	0.0	0.0	1.688821185924013E-4
8	0.0	0.0	0.0	0.0	1.688821185924013E-4
9	0.0	0.0	0.0	0.0	1.688821185924013E-4
10-11	0.0	0.0	0.0	2.814701976540022E-5	2.3924966800590187E-4
12-13	0.0	0.0	0.0	2.814701976540022E-5	8.725576127274068E-4
14-15	0.0	0.0	0.0	2.814701976540022E-5	0.001027366221437108
16-17	0.0	0.0	0.0	9.851456917890077E-5	0.0011118072807333088
18-19	0.0	0.0	0.0	1.5480860870970123E-4	0.0011821748301468092
20-21	0.0	0.0	0.0	2.53323177888602E-4	0.0012666158894430097
22-23	1.407350988270011E-5	0.0	0.0	5.910874150734046E-4	0.00129476290920841
24-25	2.814701976540022E-5	0.0	0.0	0.0014495715179181114	0.00129476290920841
26-27	2.814701976540022E-5	0.0	0.0	0.0027302609172438212	0.0014073509882700109
28-29	2.814701976540022E-5	0.0	0.0	0.009161854933637772	0.0014636450278008115
30-31	2.814701976540022E-5	0.0	0.0	0.03339643895164736	0.0014917920475662119
32-33	2.814701976540022E-5	0.0	0.0	0.07931830169889782	0.0015762331068624124
34-35	2.814701976540022E-5	2.814701976540022E-5	0.0	0.1409321279653589	0.0017169682056894133
36-37	2.814701976540022E-5	2.814701976540022E-5	0.0	0.2431621037532925	0.0017169682056894133
38-39	2.814701976540022E-5	2.814701976540022E-5	0.0	0.4123960600927613	0.001801409264985614
40-41	2.814701976540022E-5	2.814701976540022E-5	0.0	0.6057379388612955	0.001801409264985614
42-43	2.814701976540022E-5	2.814701976540022E-5	0.0	0.8037944434405341	0.0018436297946337144
44-45	2.814701976540022E-5	2.814701976540022E-5	0.0	1.040046453841421	0.0019562178736953154
46-47	2.814701976540022E-5	2.814701976540022E-5	0.0	1.3317199461603806	0.002082879462639616
48-49	2.814701976540022E-5	2.814701976540022E-5	0.0	1.6455451430347103	0.002153247012053117
50-51	2.814701976540022E-5	2.814701976540022E-5	0.0	1.9937378510425936	0.0022658350911147177
52-53	2.814701976540022E-5	2.814701976540022E-5	0.0	2.337159639200242	0.002420643699824419
54-55	2.814701976540022E-5	2.814701976540022E-5	0.0	2.719579123242852	0.00253323177888602
56-57	2.814701976540022E-5	2.814701976540022E-5	0.0	3.172394303718728	0.00258952581841682
58-59	2.814701976540022E-5	2.814701976540022E-5	0.0	3.657634850964345	0.0026176728381822204
60-61	2.814701976540022E-5	2.814701976540022E-5	0.0	4.177017733185393	0.0026458198579476207
62-63	2.814701976540022E-5	2.814701976540022E-5	0.0	4.6972309524895195	0.0027584079370092216
64-65	2.814701976540022E-5	2.814701976540022E-5	0.0	5.2444090167289	0.0027584079370092216
66-67	2.814701976540022E-5	2.814701976540022E-5	0.0	5.871609058161313	0.0027724814468919217
68-69	2.814701976540022E-5	2.814701976540022E-5	0.0	6.575650463553268	0.002786554956774622
70-71	5.629403953080044E-5	2.814701976540022E-5	0.0	7.32292569130488	0.0028006284666573216
72-73	5.629403953080044E-5	2.814701976540022E-5	0.0	8.080488654780742	0.0029835840951324233
74-75	5.629403953080044E-5	2.814701976540022E-5	0.0	8.838206426865316	0.003208760253255625
76-77	5.629403953080044E-5	2.814701976540022E-5	0.0	9.65835428878955	0.0032369072730210253
78-79	5.629403953080044E-5	2.814701976540022E-5	0.0	10.567179336484674	0.0032369072730210253
80-81	5.629403953080044E-5	2.814701976540022E-5	0.0	11.526120152872094	0.003265054292786425
82-83	5.629403953080044E-5	2.814701976540022E-5	0.0	12.480346343448808	0.0034339364113788267
84-85	5.629403953080044E-5	2.814701976540022E-5	0.0	13.439610850563533	0.0035043039607923275
86	5.629403953080044E-5	2.814701976540022E-5	0.0	14.16884383864552	0.0035183774706750276
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3935	0.0	44.44364	1
GTATCAA	7605	0.0	42.361332	1
TCAACGC	9350	0.0	34.144184	4
ATCAACG	9510	0.0	33.473263	3
CAACGCA	9655	0.0	32.828033	5
AACGCAG	10025	0.0	31.657724	6
TATCAAC	10310	0.0	31.099682	2
GTACATG	14850	0.0	28.233372	1
TACATGG	15160	0.0	27.111044	2
ACGCAGA	11690	0.0	26.675316	7
CGCAGAG	12070	0.0	25.491404	8
ACATGGG	15940	0.0	25.46678	3
CATGGGG	10390	0.0	22.691683	4
GCAGAGT	13150	0.0	22.17705	9
GTATTAG	970	0.0	19.452835	1
GAGTACA	11130	0.0	18.690205	1
AGAGTAC	11695	0.0	18.427782	10-11
GGACCGT	450	0.0	18.34501	6
GTATATG	1015	0.0	17.683546	1
CAGAGTA	12495	0.0	17.633259	10-11
>>END_MODULE