FastQCFastQC Report
Mon 15 Aug 2016
SRR2920889_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920889_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3552774
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41550.047.3819431
GTATCAA75950.040.2593461
TCAACGC96250.031.1728694
ATCAACG97500.030.7245243
CAACGCA99700.030.042745
AACGCAG104150.028.8046846
TATCAAC105400.028.6432192
GTACATG152350.028.4978351
TACATGG158250.027.2062632
ACATGGG164900.025.5941963
ACGCAGA124200.023.6185827
CGCAGAG126950.023.0682648
CATGGGG105200.022.924954
TAAGGTG14200.022.0628155
GCAGAGT137650.020.3096079
GAGTACA116150.020.266071
AGTACAT117750.018.9873922
AGAGTAC121850.018.28697610-11
GAGTACT48350.017.27436612-13
CAGAGTA132350.016.88996710-11