##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920889_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3552774
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.354118218608896	31.0	30.0	34.0	27.0	34.0
2	30.362984529834996	31.0	30.0	34.0	26.0	34.0
3	30.49862501808446	31.0	30.0	34.0	27.0	34.0
4	34.15858424994103	35.0	35.0	37.0	30.0	37.0
5	33.70825557719123	35.0	35.0	37.0	28.0	37.0
6	33.6587919749469	35.0	35.0	37.0	29.0	37.0
7	33.474394656119415	35.0	35.0	37.0	28.0	37.0
8	33.40303717602077	35.0	35.0	37.0	28.0	37.0
9	34.73903518771529	38.0	35.0	39.0	27.0	39.0
10-11	34.55271247200075	38.0	34.0	39.0	27.0	39.0
12-13	34.384925553947426	37.0	34.0	39.0	27.0	39.0
14-15	35.29131715104873	38.0	34.0	40.0	27.0	41.0
16-17	35.167157409956275	38.0	34.0	40.0	26.5	41.0
18-19	35.083278024439494	38.5	34.0	40.0	26.0	41.0
20-21	34.968639716458185	38.0	34.0	40.0	25.0	41.0
22-23	34.81894387315377	38.0	34.0	40.0	25.0	41.0
24-25	34.63331610735724	38.0	33.5	40.0	24.5	41.0
26-27	34.44770846667984	38.0	33.0	40.0	24.0	41.0
28-29	34.38228564496363	38.0	33.0	40.0	24.0	41.0
30-31	34.36044890555944	38.0	33.0	40.0	24.0	41.0
32-33	34.2028246097275	38.0	33.0	40.0	23.0	41.0
34-35	34.03154098740871	38.0	33.0	40.0	22.5	41.0
36-37	33.82627856429933	38.0	33.0	40.0	21.0	41.0
38-39	33.63153749717826	38.0	33.0	40.0	19.5	41.0
40-41	33.382638327121285	38.0	32.0	40.0	18.0	41.0
42-43	33.40040177056013	38.0	32.0	40.0	18.0	41.0
44-45	33.24478703120435	38.0	32.0	40.0	16.5	41.0
46-47	32.98099696181069	37.5	32.0	40.0	15.0	41.0
48-49	32.706377185827186	37.0	31.0	40.0	12.5	41.0
50-51	32.43929292997528	37.0	31.0	40.0	9.0	41.0
52-53	32.1703095946998	37.0	31.0	40.0	8.5	41.0
54-55	31.87517556703579	36.0	30.5	39.5	7.5	41.0
56-57	31.542666378441183	36.0	30.0	39.0	6.5	41.0
58-59	31.204146253040584	35.0	29.5	39.0	2.0	40.0
60-61	30.87205673651068	35.0	29.0	39.0	2.0	40.0
62-63	30.54406950737649	35.0	29.0	38.5	2.0	40.0
64-65	30.138390452080543	35.0	28.0	38.0	2.0	40.0
66-67	29.699962902227952	34.0	28.0	37.5	2.0	40.0
68-69	29.260957494059575	34.0	28.0	37.0	2.0	39.5
70-71	28.8394443327946	34.0	26.5	36.5	2.0	39.0
72-73	28.413501112088753	34.0	26.0	36.0	2.0	39.0
74-75	27.983621108463417	33.0	26.0	35.5	2.0	38.5
76-77	26.539553036584934	31.5	24.5	34.0	2.0	36.0
78-79	27.399148665240176	33.0	26.0	35.0	2.0	37.0
80-81	27.443359752126085	33.0	26.0	35.0	2.0	36.5
82-83	27.124072203860983	33.0	25.5	35.0	2.0	36.0
84-85	26.810113167907666	33.0	25.0	35.0	2.0	36.0
86-87	26.281797688228973	33.0	24.0	35.0	2.0	35.0
88-89	25.92616248598982	32.0	23.5	35.0	2.0	35.0
90-91	25.57005821366628	32.0	21.5	35.0	2.0	35.0
92-93	25.264183283259783	32.0	20.0	34.0	2.0	35.0
94-95	24.88718027096573	32.0	18.5	34.0	2.0	35.0
96-97	24.368556373132655	32.0	11.0	34.0	2.0	35.0
98-99	23.77804428314326	31.5	2.0	34.0	2.0	35.0
100-101	21.580436582794178	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	76268.0
3	35518.0
4	19187.0
5	13697.0
6	11858.0
7	12194.0
8	14160.0
9	15939.0
10	18023.0
11	20399.0
12	22610.0
13	24676.0
14	26799.0
15	28838.0
16	30527.0
17	32004.0
18	30115.0
19	29106.0
20	29313.0
21	30374.0
22	33441.0
23	37884.0
24	44452.0
25	51615.0
26	61657.0
27	72895.0
28	85771.0
29	101126.0
30	119412.0
31	142288.0
32	171128.0
33	209742.0
34	264496.0
35	344157.0
36	455989.0
37	519709.0
38	292978.0
39	22429.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.074407209255895	19.121047638707	13.310085310430479	24.494459841606627
2	16.209587212696334	20.65293204690194	37.69564289763436	25.441837842767367
3	19.026673606745803	24.82526173791555	29.276277174899846	26.8717874804388
4	13.639512687142997	16.330166086900956	35.69501649214579	34.33530473381026
5	14.430086569485	37.31613766220167	33.741831859169345	14.511943909143982
6	33.692483736651965	35.21063103227423	16.88057615095926	14.216309080114547
7	29.497364079491124	31.772799239623676	20.72919006786618	18.00064661301902
8	27.401803365775507	33.9227503371271	19.870419193064976	18.80502710403242
9	27.291620392659148	15.214865484983545	19.169559614263633	38.32395450809368
10-11	25.99825484430058	25.187883214620193	27.32657955541503	21.48728238566419
12-13	26.437775823380612	22.633473788800554	27.39092956195422	23.53782082586462
14-15	23.652049382034164	24.221524517217052	24.665514199399006	27.460911901349778
16-17	23.751870407599224	27.324956254466976	25.475694383320015	23.44747895461379
18-19	23.86422072568913	26.19295680437961	27.02541488041171	22.917407589519563
20-21	24.623637278257878	25.47834378861599	26.488566674302167	23.40945225882396
22-23	24.322047040797397	25.296116048784867	26.580826782517125	23.80101012790061
24-25	23.96831460981111	25.7569124149788	26.552609889123385	23.72216308608671
26-27	23.738906587399622	25.811160356487644	26.516722311235068	23.93321074487767
28-29	23.873727118545276	25.816822210952463	26.59145556280487	23.717995107697394
30-31	23.88597845221077	25.742815937227476	26.49150553686823	23.879700073693524
32-33	23.606289337926196	25.790813036835814	26.254899907027767	24.347997718210227
34-35	24.158394461945512	25.823552356548994	26.263472846536978	23.754580334968516
36-37	24.23220293649788	25.482537269310356	26.345214465700973	23.940045328490786
38-39	24.13053559497272	25.508681621688133	26.26552723109667	24.095255552242477
40-41	23.99627857949173	25.556316187855437	26.551074018907507	23.896331213745327
42-43	24.140387443738653	25.460383440679777	26.24165554353981	24.157573572041755
44-45	23.90300988466576	25.51275929369181	26.402992991651473	24.181237829990955
46-47	24.084246531564293	25.52285227010676	26.24618861341129	24.14671258491765
48-49	23.843626990859416	25.6671267710818	26.027032858548928	24.462213379509855
50-51	23.668383356051738	25.887228315183947	26.216279215108667	24.228109113655645
52-53	24.06486859877966	25.421308245899304	26.277654368267395	24.236168787053643
54-55	23.71913519861881	25.440819163433794	26.40467166829894	24.43537396964846
56-57	23.646402665774172	25.726237963355736	26.15377123556933	24.473588135300762
58-59	23.50089511402852	25.389332380046277	26.287190861955402	24.8225816439698
60-61	23.393770709787837	25.499720080458037	26.47575736289749	24.63075184685663
62-63	23.154422211256232	25.57623767839011	26.47969807428618	24.789642036067477
64-65	23.244189969397596	25.4921149157417	26.33578874136048	24.927906373500228
66-67	23.2351951476719	25.658923460099654	26.1647966862699	24.941084705958545
68-69	23.55287900043513	25.329817161803852	26.106728418008768	25.01057541975225
70-71	23.132927522628545	25.27567335949805	26.25581558703311	25.335583530840296
72-73	23.20817051099626	25.214894509147857	26.125850109302284	25.451084870553604
74-75	23.15983147092203	25.363891049114777	26.043365628964732	25.432911850998458
76-77	23.081245216876095	25.093172608614385	26.3354693545031	25.490112820006416
78-79	23.027909433430754	25.050501778231627	26.268657347089686	25.652931441247933
80-81	23.00096758818861	25.100552316341933	26.25118555593811	25.64729453953134
82-83	22.959299220018426	25.0733638719966	26.158638244925335	25.808698663059644
84-85	22.582686563802863	25.048729404014896	26.266598541214492	26.10198549096775
86-87	22.75917971133706	24.910952443772526	26.18638011542849	26.143487729461917
88-89	22.60266727387583	24.8569171361988	26.01957824735475	26.520837342570612
90-91	22.448674043292012	24.867091062747974	26.138444863481443	26.545790030478567
92-93	22.502511787819532	24.733999057143915	26.140063559459676	26.623425595576883
94-95	22.181469512591587	24.443631923515696	26.475576943808548	26.899321620084166
96-97	22.333510666745482	24.161058145655474	26.42280507680226	27.08262611079679
98-99	22.224216039325018	24.23243553860295	26.289365162535738	27.253983259536298
100-101	22.32490478421579	24.04674788537991	26.358808513848427	27.269538816555873
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	248.0
1	177.0
2	157.0
3	235.0
4	199.5
5	184.5
6	314.5
7	598.0
8	928.5
9	1020.0
10	844.0
11	687.0
12	663.0
13	683.5
14	723.0
15	791.5
16	894.0
17	969.5
18	1058.0
19	1198.5
20	1319.0
21	1537.5
22	1876.0
23	2203.5
24	2680.5
25	3416.5
26	4515.0
27	6289.0
28	8700.5
29	12250.5
30	17284.0
31	23826.5
32	31852.0
33	37893.5
34	43189.5
35	49926.0
36	58476.0
37	69025.5
38	77851.0
39	83789.5
40	89167.5
41	95337.0
42	102658.0
43	111259.0
44	120132.5
45	128928.5
46	137058.0
47	143881.0
48	148946.5
49	152576.0
50	154682.5
51	155560.5
52	154929.5
53	155720.0
54	154366.0
55	145707.5
56	134399.0
57	122193.5
58	111756.5
59	99861.5
60	84390.0
61	70112.5
62	56966.0
63	42828.0
64	30733.5
65	23370.5
66	18324.5
67	14016.5
68	10433.0
69	7936.0
70	6141.0
71	4597.5
72	3446.5
73	3027.0
74	2402.0
75	1711.5
76	1180.0
77	752.0
78	446.5
79	208.0
80	114.5
81	59.5
82	39.5
83	27.0
84	14.5
85	7.0
86	5.5
87	5.0
88	3.5
89	1.0
90	1.0
91	0.5
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18762803375615786
2	0.0
3	0.012975776111849502
4	0.013426128428095903
5	0.007093048980880856
6	0.10816899695843304
7	0.14233947895362892
8	0.12410021014564956
9	0.1425928021315175
10-11	0.11521982540966579
12-13	0.1148679876625983
14-15	0.11696494063512061
16-17	0.06787653816426263
18-19	0.08065528513775433
20-21	0.0388288137663696
22-23	0.08515880830021835
24-25	0.059545020313704164
26-27	0.06790468518402802
28-29	0.04168573627255773
30-31	0.025374538318508298
32-33	0.03310089524411066
34-35	0.02163098468971007
36-37	0.026007846263229804
38-39	0.03352310054059166
40-41	0.025458979377804496
42-43	0.01380611319492881
44-45	0.02842848996305422
46-47	0.03504303960792327
48-49	0.00899297281504537
50-51	0.01655044762205533
52-53	0.006713064214047952
54-55	0.013144658230441903
56-57	0.0052071986565990405
58-59	0.004334641043871634
60-61	0.02422051050812689
62-63	0.010611426451555883
64-65	0.007754503945367761
66-67	0.04191091243068093
68-69	0.09202668112297602
70-71	0.022222072104783476
72-73	0.045401142881590556
74-75	0.08780462815816599
76-77	0.23675865675666397
78-79	0.08159821029989524
80-81	0.01809853370915234
82-83	0.012567644325251197
84-85	0.014186097961761712
86-87	0.2539283388135581
88-89	0.6154486606803584
90-91	0.26639746856963037
92-93	0.35090889541524456
94-95	0.0012666158894430097
96-97	0.004053170846217632
98-99	0.0035043039607923275
100-101	0.005221272166481741
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3552774.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.40999099090525
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.79932934123728	24.685729705038675
2	11.401380707157063	8.302483376629393
3	5.676073747181212	6.199973819957451
4	3.556637471075467	5.179885531190951
5	2.3346988355678366	4.250318178475096
6	1.693850137854783	3.700384095554176
7	1.2749433102694618	3.249447210857822
8	0.9398646313175665	2.7376370207154466
9	0.784038427483336	2.5692148873072607
>10	4.261749086462776	27.280302190941246
>50	0.1921916206709227	4.752212520359581
>100	0.0791751981010222	5.070481896964864
>500	0.00435614352546519	1.0500359549533367
>1k	0.0017113420958833208	0.9718936110547163
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1258807906160088E-4
2	0.0	0.0	0.0	0.0	1.1258807906160088E-4
3	0.0	0.0	0.0	0.0	1.1258807906160088E-4
4	0.0	0.0	0.0	2.814701976540022E-5	1.1258807906160088E-4
5	0.0	2.814701976540022E-5	0.0	2.814701976540022E-5	1.688821185924013E-4
6	2.814701976540022E-5	2.814701976540022E-5	0.0	2.814701976540022E-5	1.9702913835780155E-4
7	2.814701976540022E-5	2.814701976540022E-5	0.0	2.814701976540022E-5	1.9702913835780155E-4
8	2.814701976540022E-5	2.814701976540022E-5	0.0	2.814701976540022E-5	1.9702913835780155E-4
9	2.814701976540022E-5	2.814701976540022E-5	0.0	2.814701976540022E-5	1.9702913835780155E-4
10-11	2.814701976540022E-5	2.814701976540022E-5	0.0	5.629403953080044E-5	2.53323177888602E-4
12-13	2.814701976540022E-5	2.814701976540022E-5	0.0	5.629403953080044E-5	6.47381454604205E-4
14-15	2.814701976540022E-5	2.814701976540022E-5	0.0	5.629403953080044E-5	8.021900633139064E-4
16-17	2.814701976540022E-5	2.814701976540022E-5	0.0	8.444105929620065E-5	9.147781423755072E-4
18-19	2.814701976540022E-5	2.814701976540022E-5	0.0	1.1258807906160088E-4	9.569986720236075E-4
20-21	2.814701976540022E-5	2.814701976540022E-5	0.0	2.1110264824050165E-4	0.0012525423795603098
22-23	2.814701976540022E-5	2.814701976540022E-5	0.0	5.20719865659904E-4	0.0013510569487392105
24-25	2.814701976540022E-5	4.2220529648100334E-5	0.0	0.0013229099289738104	0.0013792039685046108
26-27	2.814701976540022E-5	5.629403953080044E-5	0.0	0.0027161874073611215	0.0015058655574489118
28-29	2.814701976540022E-5	5.629403953080044E-5	0.0	0.009133707913872371	0.001548086087097012
30-31	2.814701976540022E-5	5.629403953080044E-5	0.0	0.033171262793524156	0.0016184536365105126
32-33	2.814701976540022E-5	5.629403953080044E-5	0.0	0.0790931255407746	0.0017451152254548137
34-35	2.814701976540022E-5	5.629403953080044E-5	0.0	0.1406225107479395	0.0018577033045164145
36-37	4.2220529648100334E-5	5.629403953080044E-5	0.0	0.2432606183224714	0.0018577033045164145
38-39	7.036754941350055E-5	5.629403953080044E-5	0.0	0.41325454419560603	0.0019702913835780156
40-41	8.444105929620065E-5	5.629403953080044E-5	0.0	0.6082993176599468	0.0019702913835780156
42-43	8.444105929620065E-5	5.629403953080044E-5	0.0	0.8074817030298015	0.0020125119132261156
44-45	8.444105929620065E-5	5.629403953080044E-5	0.0	1.0457321518340317	0.0021250999922877165
46-47	8.444105929620065E-5	5.629403953080044E-5	0.0	1.339263347457508	0.0022939821108801176
48-49	8.444105929620065E-5	5.629403953080044E-5	0.0	1.6518782224819253	0.002406570189941719
50-51	8.444105929620065E-5	7.036754941350055E-5	0.0	1.9958770245447641	0.0025050847591206195
52-53	8.444105929620065E-5	8.444105929620065E-5	0.0	2.3349078776190098	0.002673966877713021
54-55	8.444105929620065E-5	8.444105929620065E-5	0.0	2.7113742669812377	0.0027443344271265214
56-57	8.444105929620065E-5	8.444105929620065E-5	0.0	3.159094836879576	0.0028147019765400218
58-59	8.444105929620065E-5	8.444105929620065E-5	0.0	3.638874862290706	0.0028709960160708224
60-61	8.444105929620065E-5	8.444105929620065E-5	0.0	4.1535853392306965	0.0028709960160708224
62-63	8.444105929620065E-5	8.444105929620065E-5	0.0	4.669843902257785	0.003039878134663224
64-65	8.444105929620065E-5	8.444105929620065E-5	0.0	5.213531736046256	0.003068025154428624
66-67	8.444105929620065E-5	8.444105929620065E-5	0.0	5.833695022537318	0.0031524662137248248
68-69	8.444105929620065E-5	8.444105929620065E-5	0.0	6.527983485580563	0.003222833763138325
70-71	8.444105929620065E-5	8.444105929620065E-5	0.0	7.267546429916454	0.0033072748224345257
72-73	8.444105929620065E-5	8.444105929620065E-5	0.0	8.017565992095191	0.003504303960792327
74-75	8.444105929620065E-5	8.444105929620065E-5	0.0	8.771807607238738	0.0036591125695020284
76-77	8.444105929620065E-5	8.444105929620065E-5	0.0	9.589548898973028	0.0036591125695020284
78-79	8.444105929620065E-5	8.444105929620065E-5	0.0	10.491703102983752	0.003715406609032829
80-81	8.444105929620065E-5	8.444105929620065E-5	0.0	11.445915220050587	0.0037435536287982294
82-83	8.444105929620065E-5	8.444105929620065E-5	0.0	12.394371271575395	0.0037998476683290296
84-85	8.444105929620065E-5	8.444105929620065E-5	0.0	13.348428580033517	0.00382799468809443
86-87	8.444105929620065E-5	8.444105929620065E-5	0.0	14.325102018873139	0.003940582767156031
88-89	8.444105929620065E-5	8.444105929620065E-5	0.0	15.370243083292099	0.004053170846217632
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4155	0.0	47.381943	1
GTATCAA	7595	0.0	40.259346	1
TCAACGC	9625	0.0	31.172869	4
ATCAACG	9750	0.0	30.724524	3
CAACGCA	9970	0.0	30.04274	5
AACGCAG	10415	0.0	28.804684	6
TATCAAC	10540	0.0	28.643219	2
GTACATG	15235	0.0	28.497835	1
TACATGG	15825	0.0	27.206263	2
ACATGGG	16490	0.0	25.594196	3
ACGCAGA	12420	0.0	23.618582	7
CGCAGAG	12695	0.0	23.068264	8
CATGGGG	10520	0.0	22.92495	4
TAAGGTG	1420	0.0	22.062815	5
GCAGAGT	13765	0.0	20.309607	9
GAGTACA	11615	0.0	20.26607	1
AGTACAT	11775	0.0	18.987392	2
AGAGTAC	12185	0.0	18.286976	10-11
GAGTACT	4835	0.0	17.274366	12-13
CAGAGTA	13235	0.0	16.889967	10-11
>>END_MODULE