FastQCFastQC Report
Mon 15 Aug 2016
SRR2920888_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920888_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5104919
Sequences flagged as poor quality0
Sequence length98
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58400.045.7863771
GGTATCA32450.040.1369821
ATCAACG73000.035.498913
TCAACGC72650.035.4805344
CAACGCA76800.033.8021775
TATCAAC78900.032.7865562
AACGCAG81050.032.363216
GTACATG137500.028.7182241
ACGCAGA95000.027.0749077
TACATGG144350.026.6904662
ACATGGG149350.024.9060613
CGCAGAG102750.024.6753068
CATGGGG103700.021.8493044
GCAGAGT120550.019.7007059
GAGTACA103500.019.1650471
AGTACAT100750.018.0278432
AGAGTAC100800.017.78450810-11
ATGGGGA63050.017.3861035
CAGAGTA110500.015.9328910-11
TAATACT22250.015.4603994
Produced by FastQC (version 0.11.5)