##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920888_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5104919
Sequences flagged as poor quality	0
Sequence length	98
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.757007897676733	31.0	30.0	33.0	23.0	34.0
2	28.785110988048977	31.0	28.0	34.0	22.0	34.0
3	28.860440488869656	31.0	28.0	34.0	22.0	34.0
4	32.35851871498843	35.0	33.0	37.0	25.0	37.0
5	32.321437813215056	35.0	32.0	37.0	25.0	37.0
6	32.44776636024979	35.0	33.0	37.0	25.0	37.0
7	32.28375396357905	35.0	32.0	37.0	23.0	37.0
8	32.227326231816804	35.0	32.0	37.0	23.0	37.0
9	33.50613751168236	37.0	33.0	39.0	23.0	39.0
10-11	33.30945907271007	37.0	33.0	39.0	22.0	39.0
12-13	33.13374893901353	37.0	32.0	39.0	19.5	39.0
14-15	33.91017389306275	38.0	33.0	40.0	19.0	41.0
16-17	33.79470242329016	38.0	32.5	40.0	18.0	41.0
18-19	33.66812421509529	38.0	32.0	40.0	18.0	41.0
20-21	33.57195667551238	38.0	32.0	40.0	18.0	41.0
22-23	33.34869671781276	38.0	32.0	40.0	17.5	41.0
24-25	33.148107540981556	38.0	32.0	40.0	16.5	41.0
26-27	33.210757996356065	38.0	32.0	40.0	16.0	41.0
28-29	33.05847458108542	38.0	32.0	40.0	15.5	41.0
30-31	32.82328250458038	38.0	31.5	40.0	10.5	41.0
32-33	32.59612130966231	37.5	31.0	40.0	9.5	41.0
34-35	32.26578511823596	37.0	30.5	40.0	8.5	41.0
36-37	31.965819242185823	37.0	30.0	40.0	8.0	41.0
38-39	31.776197624291395	37.0	30.0	40.0	7.5	41.0
40-41	31.592235743603375	37.0	30.0	40.0	2.0	41.0
42-43	31.368333464252814	37.0	30.0	40.0	2.0	41.0
44-45	31.102898596432183	36.0	29.5	39.5	2.0	41.0
46-47	30.86099005684517	36.0	29.0	39.0	2.0	40.0
48-49	30.577988602757458	36.0	28.0	39.0	2.0	40.0
50-51	29.433924220932788	34.0	27.0	38.0	2.0	39.5
52-53	29.899814179225956	35.0	28.0	38.5	2.0	39.5
54-55	30.564227365801493	36.0	29.0	39.0	2.0	40.0
56-57	30.52154049848783	36.0	28.5	39.0	2.0	41.0
58-59	30.27794917020231	35.0	28.0	39.0	2.0	41.0
60-61	29.957706772624597	35.0	28.0	39.0	2.0	40.0
62-63	29.566577452061434	35.0	28.0	39.0	2.0	40.0
64-65	29.14067774630704	34.0	26.5	38.0	2.0	40.0
66-67	28.760303052800644	34.0	26.0	38.0	2.0	40.0
68-69	28.314926348488584	34.0	26.0	37.0	2.0	40.0
70-71	27.980943674130774	34.0	26.0	37.0	2.0	39.0
72-73	27.511757189487238	33.5	25.0	36.0	2.0	39.0
74-75	27.059238354222664	33.0	24.0	36.0	2.0	38.5
76-77	26.63588746461991	33.0	23.5	35.0	2.0	37.5
78-79	26.16389486297432	32.5	22.0	35.0	2.0	37.0
80-81	25.705553604278542	32.0	20.0	35.0	2.0	36.5
82-83	25.22211890139687	32.0	18.5	35.0	2.0	36.0
84-85	24.81696810468491	32.0	17.0	35.0	2.0	35.5
86-87	24.35400796760928	31.0	9.0	34.0	2.0	35.0
88-89	23.81536455328674	31.0	4.5	34.0	2.0	35.0
90-91	23.31299752258557	31.0	2.0	34.0	2.0	35.0
92-93	22.762842760090805	30.5	2.0	34.0	2.0	35.0
94-95	22.11611104897061	30.0	2.0	34.0	2.0	35.0
96-97	21.460103676473615	29.5	2.0	34.0	2.0	35.0
98	21.056210098534375	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	235809.0
3	42040.0
4	31473.0
5	25575.0
6	23953.0
7	24384.0
8	27838.0
9	31868.0
10	35224.0
11	38432.0
12	39278.0
13	38949.0
14	36917.0
15	38104.0
16	40864.0
17	43880.0
18	45610.0
19	46417.0
20	48668.0
21	51651.0
22	56539.0
23	63332.0
24	72050.0
25	82404.0
26	95777.0
27	110664.0
28	129049.0
29	149128.0
30	173857.0
31	203741.0
32	241650.0
33	292996.0
34	361564.0
35	459035.0
36	583383.0
37	649629.0
38	394199.0
39	38986.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.857826610067214	17.527537965042647	12.295005089664413	25.319630335225728
2	16.276024114137744	19.505193066715155	38.50761639335011	25.71116642579699
3	19.162021451031418	23.237768360757265	28.804797494982854	28.79541269322846
4	13.27018312828031	15.383551388905486	35.97394814039698	35.372317342417226
5	14.731955585572187	36.50637745680358	34.078988024108135	14.682678933516097
6	34.40229197481602	34.40399661345124	17.093920298542965	14.099791113189768
7	30.158022362582926	30.97431874566407	20.633245372247018	18.23441351950599
8	27.00639495691147	34.086826678703126	20.017177030971354	18.889601333414046
9	27.110929673908636	15.337559714463639	19.096111025463873	38.45539958616386
10-11	25.87841797984531	25.123057852470048	27.504624953672106	21.493899214012533
12-13	26.50517924883971	22.26347763935604	27.36515014400107	23.866192967803176
14-15	23.520214466497936	23.844921210791497	24.705294666154725	27.92956965655584
16-17	24.232562736359924	26.961180675877838	25.24351640778269	23.56274017997955
18-19	24.05027001048884	26.06585189129818	26.6023651576486	23.28151294056438
20-21	24.615032192067904	25.620639779366417	26.250444525388033	23.513883503177645
22-23	24.3848158530911	25.37758503616781	26.046842956350584	24.1907561543905
24-25	24.28281102560072	25.485901239088342	26.13329912220625	24.09798861310469
26-27	24.022928276652685	25.86374571376371	25.967377948612995	24.14594806097061
28-29	24.05561914692612	25.925927377783704	26.12966040901447	23.88879306627571
30-31	24.123776784953947	25.598675808067426	25.994978912864763	24.28256849411386
32-33	24.01772075537056	25.708741558145505	25.877953357183447	24.395584329300483
34-35	24.548082706778	25.677754331872105	25.69914750608359	24.075015455266303
36-37	24.46708841830927	25.42729647273986	25.74276851276778	24.36284659618309
38-39	24.229899634333506	25.602686493995254	26.012062536734177	24.155351334937066
40-41	24.355239219822554	25.30010666588464	26.114306189052023	24.230347925240785
42-43	24.296363604709114	25.299887012140466	25.889800295655007	24.51394908749541
44-45	24.167562173477634	25.495452582324347	25.88659107697609	24.450394167221933
46-47	24.134791146175502	25.48587287787843	25.770803839448643	24.608532136497427
48-49	24.232860917224876	25.426009757845325	25.838556536597128	24.502572788332667
50-51	23.997563618272665	25.514579945373132	26.00179729854623	24.486059137807963
52-53	24.192290222127383	25.37461239148954	25.975798491486067	24.45729889489701
54-55	23.725926471033848	25.176270630166762	26.29166419072527	24.806138708074112
56-57	23.77439076805114	25.468292321180265	26.2845717628587	24.472745147909894
58-59	23.833316943652278	25.10072770183892	26.268000786704693	24.797954567804112
60-61	23.860654941210765	25.04817306266196	26.474255048533035	24.616916947594238
62-63	23.715146995427926	25.170843048593603	26.415635354422136	24.69837460155633
64-65	23.5472807187036	24.970219953241294	26.363054482433217	25.119444845621892
66-67	23.56352094489931	25.17817919606964	26.245069875371485	25.013229983659567
68-69	23.514860759760072	24.882819518603373	26.478348520581978	25.123971201054573
70-71	23.347871164675027	24.878083833418412	26.4848243383881	25.289220663518456
72-73	23.423425194373852	24.81497331291045	26.51021091764588	25.25139057506982
74-75	23.351077540735204	24.88580128957597	26.39406055659139	25.36906061309743
76-77	23.322589581302235	24.69614481787655	26.610739801616468	25.370525799204746
78-79	23.24247361210704	24.648288357802446	26.63443971956817	25.474798310522345
80-81	23.150564674287914	24.779876983112157	26.681034559013884	25.38852378358605
82-83	23.189335323037298	24.6630286166709	26.599009655777543	25.54862640451426
84-85	23.127511673230146	24.455316418001395	26.69645866973825	25.720713239030214
86-87	23.183456015735214	24.340573082143294	26.89434154198946	25.58162936013204
88-89	22.995381466487615	24.224483536274853	26.940472234729246	25.83966276250828
90-91	22.958453690150076	24.294914392308197	27.03024819198171	25.716383725560014
92-93	23.088246176924777	23.964863372903526	27.089199077251262	25.85769137292043
94-95	22.910822033271952	23.88326550413501	27.165259761192996	26.04065270140004
96-97	23.125272062746944	23.69363933637379	27.157319371221405	26.023769229657862
98	23.015324837574333	23.6168904610031	27.077548796495293	26.29023590492728
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	52.0
1	57.5
2	113.5
3	177.5
4	177.0
5	217.5
6	354.5
7	651.5
8	976.5
9	1149.5
10	1232.0
11	1359.0
12	1604.0
13	1875.5
14	2109.0
15	2254.5
16	2396.5
17	2471.0
18	2440.5
19	2461.0
20	2457.5
21	2452.5
22	2585.0
23	2813.0
24	3347.0
25	4236.5
26	5495.0
27	7515.5
28	10441.0
29	14688.0
30	20936.0
31	29574.5
32	39593.0
33	49989.0
34	61405.0
35	74430.5
36	89306.5
37	105128.0
38	117850.5
39	129843.5
40	145093.0
41	160396.0
42	174912.0
43	186793.5
44	197382.5
45	205975.0
46	212816.5
47	226654.5
48	237913.0
49	239421.5
50	239246.0
51	238563.5
52	237196.0
53	233729.5
54	224491.5
55	211275.5
56	193037.5
57	171749.0
58	153838.5
59	132991.0
60	109978.0
61	90662.0
62	75175.0
63	59654.5
64	44292.5
65	33610.5
66	26244.0
67	20366.5
68	14857.5
69	11155.5
70	8858.0
71	7540.0
72	6301.0
73	4569.5
74	3438.5
75	2371.0
76	1447.5
77	851.5
78	468.0
79	230.5
80	131.0
81	88.0
82	52.5
83	33.0
84	19.5
85	15.0
86	14.0
87	13.0
88	9.0
89	7.5
90	7.5
91	10.0
92	8.0
93	4.0
94	4.5
95	3.5
96	3.0
97	2.0
98	0.5
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.37457989049385504
2	0.023330438739576475
3	0.018061011350033174
4	0.015514447927577304
5	0.021195243254594244
6	0.02346756138540102
7	0.015044313141893143
8	0.008384070344700865
9	0.0
10-11	5.876684821052009E-5
12-13	0.005279221864245055
14-15	0.003114642955157565
16-17	0.005553467155894149
18-19	0.03675866355568031
20-21	0.07782689598013211
22-23	0.09680858795213008
24-25	0.06395791980244936
26-27	0.07261623543879933
28-29	0.056788364320765915
30-31	0.05166585405174891
32-33	0.018815185902068182
34-35	0.33002482507557906
36-37	0.5066387928975955
38-39	0.4632688589182316
40-41	0.47210347509921313
42-43	0.42369918112314814
44-45	0.39716594915609826
46-47	0.39527561553865986
48-49	0.38546155188750303
50-51	0.38216081391301215
52-53	0.4433468973748653
54-55	0.4442186056233213
56-57	0.41493312626507883
58-59	0.3999867578702032
60-61	0.4146001141252192
62-63	0.3944234962396073
64-65	0.44382682663525125
66-67	0.46343536498816146
68-69	0.40695062938314985
70-71	0.36307138271929484
72-73	0.3489771336234718
74-75	0.3323754990039999
76-77	0.3299856471767721
78-79	0.32982893558154397
80-81	0.34574495697189317
82-83	0.340749774873999
84-85	0.3413178544067007
86-87	0.3273901113808074
88-89	0.37001566528283797
90-91	0.34845802646427887
92-93	0.338761496509543
94-95	0.34537276693322655
96-97	0.3453042056103143
98	0.34008375059427975
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	5104919.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.61225379565605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.59251926165352	39.0150028918548
2	12.108719173644811	12.499165742609652
3	4.8076813388539765	7.444058082887127
4	2.4175538601137725	4.9910161357144
5	1.3452295017216664	3.4715163228131285
6	0.8232604606070295	2.549419669966723
7	0.5730514601705425	2.0703534180205327
8	0.39055533260571224	1.6125952758154305
9	0.29049504719774794	1.3493793692116123
>10	1.487764215157653	13.72980174349927
>50	0.10107600989960476	3.577148924693708
>100	0.05734369621493543	5.5689266230367505
>500	0.003562983394252695	1.2551425057031411
>1k	0.0011876587648555308	0.8664732941738192
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	1.9588949403506695E-5
10-11	0.0	0.0	0.0	4.897237350876674E-5	1.9588949403506695E-5
12-13	0.0	0.0	0.0	5.876684821052009E-5	8.815027231578014E-5
14-15	0.0	0.0	0.0	5.876684821052009E-5	1.1753369642104018E-4
16-17	0.0	0.0	0.0	5.876684821052009E-5	1.1753369642104018E-4
18-19	0.0	0.0	0.0	5.876684821052009E-5	1.1753369642104018E-4
20-21	0.0	0.0	0.0	6.856132291227344E-5	1.1753369642104018E-4
22-23	0.0	0.0	0.0	2.25272918140327E-4	1.1753369642104018E-4
24-25	0.0	0.0	0.0	5.484905832981875E-4	1.1753369642104018E-4
26-27	0.0	0.0	0.0	0.0011165701159998817	1.2732817112279352E-4
28-29	0.0	0.0	0.0	0.005543672681192395	1.3712264582454688E-4
30-31	0.0	0.0	0.0	0.027091517025049763	1.3712264582454688E-4
32-33	0.0	9.794474701753347E-6	0.0	0.07155843217100996	1.3712264582454688E-4
34-35	0.0	1.9588949403506695E-5	0.0	0.1289050815497758	1.5671159522805356E-4
36-37	0.0	1.9588949403506695E-5	0.0	0.22770194786636183	1.5671159522805356E-4
38-39	0.0	1.9588949403506695E-5	0.0	0.3972443049537123	1.5671159522805356E-4
40-41	0.0	1.9588949403506695E-5	0.0	0.5945539978205334	1.5671159522805356E-4
42-43	0.0	1.9588949403506695E-5	0.0	0.8090334048395282	1.5671159522805356E-4
44-45	0.0	1.9588949403506695E-5	0.0	1.06461042770708	1.5671159522805356E-4
46-47	0.0	1.9588949403506695E-5	0.0	1.362401636539189	1.5671159522805356E-4
48-49	0.0	1.9588949403506695E-5	0.0	1.68774470270733	1.6650606992980692E-4
50-51	0.0	1.9588949403506695E-5	0.0	2.042627904575959	1.7630054463156026E-4
52-53	0.0	1.9588949403506695E-5	0.0	2.407168458500517	2.0568396873682032E-4
54-55	0.0	3.917789880701339E-5	0.0	2.815186685626158	2.1547844343857366E-4
56-57	0.0	3.917789880701339E-5	0.0	3.28549777185495	2.25272918140327E-4
58-59	0.0	5.876684821052009E-5	0.0	3.7950455237389664	2.3506739284208036E-4
60-61	0.0	5.876684821052009E-5	0.0	4.335739705174558	2.3506739284208036E-4
62-63	0.0	5.876684821052009E-5	0.0	4.8805475659848865	2.3506739284208036E-4
64-65	0.0	5.876684821052009E-5	0.0	5.464425586380509	2.3506739284208036E-4
66-67	0.0	7.835579761402678E-5	0.0	6.12739398999279	2.448618675438337E-4
68-69	0.0	7.835579761402678E-5	0.0	6.842439615594293	2.5465634224558704E-4
70-71	0.0	7.835579761402678E-5	0.0	7.605497756183791	2.5465634224558704E-4
72-73	9.794474701753347E-6	7.835579761402678E-5	0.0	8.373345394902445	2.5465634224558704E-4
74-75	1.9588949403506695E-5	7.835579761402678E-5	0.0	9.175973213287028	2.5465634224558704E-4
76-77	1.9588949403506695E-5	7.835579761402678E-5	9.794474701753347E-6	10.039620609063533	2.7424529164909377E-4
78-79	1.9588949403506695E-5	8.815027231578013E-5	1.9588949403506695E-5	10.970585429465189	2.840397663508471E-4
80-81	1.9588949403506695E-5	1.0773922171928683E-4	1.9588949403506695E-5	11.931599698251823	2.9383424105260044E-4
82-83	1.9588949403506695E-5	1.1753369642104018E-4	1.9588949403506695E-5	12.905385962049545	2.9383424105260044E-4
84-85	1.9588949403506695E-5	1.1753369642104018E-4	1.9588949403506695E-5	13.893687637355264	2.9383424105260044E-4
86	1.9588949403506695E-5	1.1753369642104018E-4	1.9588949403506695E-5	14.65159388425164	2.9383424105260044E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5840	0.0	45.786377	1
GGTATCA	3245	0.0	40.136982	1
ATCAACG	7300	0.0	35.49891	3
TCAACGC	7265	0.0	35.480534	4
CAACGCA	7680	0.0	33.802177	5
TATCAAC	7890	0.0	32.786556	2
AACGCAG	8105	0.0	32.36321	6
GTACATG	13750	0.0	28.718224	1
ACGCAGA	9500	0.0	27.074907	7
TACATGG	14435	0.0	26.690466	2
ACATGGG	14935	0.0	24.906061	3
CGCAGAG	10275	0.0	24.675306	8
CATGGGG	10370	0.0	21.849304	4
GCAGAGT	12055	0.0	19.700705	9
GAGTACA	10350	0.0	19.165047	1
AGTACAT	10075	0.0	18.027843	2
AGAGTAC	10080	0.0	17.784508	10-11
ATGGGGA	6305	0.0	17.386103	5
CAGAGTA	11050	0.0	15.93289	10-11
TAATACT	2225	0.0	15.460399	4
>>END_MODULE