FastQCFastQC Report
Mon 15 Aug 2016
SRR2920888_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920888_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5104919
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34600.046.598181
GTATCAA62700.043.2367171
TCAACGC79750.033.873434
ATCAACG81750.033.10283
CAACGCA83600.032.4793785
AACGCAG87300.031.266566
TATCAAC90800.029.9585532
GTACATG144950.026.3476981
ACGCAGA107200.025.1521877
TACATGG150600.024.7140052
CGCAGAG115050.023.0220628
ACATGGG155400.022.913313
CATGGGG107100.019.5934264
GCAGAGT133000.019.2725329
GAGTACA117500.019.1455841
AGTACAT112450.018.4912682
AGAGTAC112900.018.07682410-11
GTAATAC8300.016.588133
TAGCGCG2901.0363201E-616.3711644
CTATACG2104.0216115E-415.825464
Produced by FastQC (version 0.11.5)