FastQCFastQC Report
Mon 15 Aug 2016
SRR2920887_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920887_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3125906
Sequences flagged as poor quality0
Sequence length98
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA66700.042.4327051
GGTATCA33600.041.2267071
ATCAACG82350.034.0915643
TCAACGC84000.033.476524
TATCAAC83450.033.3139572
CAACGCA85400.032.5517125
AACGCAG89500.031.1580416
GTACATG129150.026.8319361
TACATGG131000.026.0194112
ACGCAGA104600.025.9092627
CGCAGAG106550.025.218298
ACATGGG136950.024.1508453
GCAGAGT119650.021.3855679
CATGGGG80550.021.2423424
GTGGTAT21300.018.7971121
CATGGGA52400.018.0341224
AGAGTAC101250.018.025510-11
TAAGGCG5600.018.0216165
GAGTACA100350.017.6561011
AGTACAT94200.017.5320012
Produced by FastQC (version 0.11.5)