FastQCFastQC Report
Mon 15 Aug 2016
SRR2920887_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920887_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3125906
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37650.044.4628871
GTATCAA69000.041.699061
ATCAACG92600.030.6560553
TCAACGC93200.030.3568294
TATCAAC94200.030.2337252
CAACGCA94500.029.7344745
AACGCAG100200.028.1851026
GTACATG137150.024.1689031
TACATGG139750.023.4362742
ACGCAGA116700.023.3459437
CGCAGAG118500.022.8685938
ACATGGG143400.022.6098463
GCAGAGT131250.019.8515649
AGAGTAC109700.019.2935310-11
CATGGGG85600.018.7997474
GAGTACA107250.017.204991
AGTACAT103250.016.734112
CATGGGA55900.016.4746424
CAGAGTA122800.016.26923610-11
GTGGTAT22000.016.212821