##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920887_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3125906
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.248446050521032	31.0	30.0	34.0	27.0	34.0
2	30.2582985540832	31.0	30.0	34.0	26.0	34.0
3	30.394892552751106	31.0	30.0	34.0	27.0	34.0
4	34.04324730174228	35.0	35.0	37.0	30.0	37.0
5	33.575108464553956	35.0	35.0	37.0	28.0	37.0
6	33.51992414359229	35.0	35.0	37.0	28.0	37.0
7	33.33594996138719	35.0	33.0	37.0	27.0	37.0
8	33.2697998596247	35.0	33.0	37.0	27.0	37.0
9	34.590765685212546	38.0	34.0	39.0	27.0	39.0
10-11	34.40322101816241	37.5	34.0	39.0	27.0	39.0
12-13	34.22122610212847	37.0	34.0	39.0	26.5	39.0
14-15	35.114726098609495	38.0	34.0	40.0	26.0	41.0
16-17	34.98946129538125	38.0	34.0	40.0	25.5	41.0
18-19	34.907450351993944	38.0	34.0	40.0	25.0	41.0
20-21	34.79152939979642	38.0	34.0	40.0	25.0	41.0
22-23	34.64542951707441	38.0	34.0	40.0	24.5	41.0
24-25	34.46754524928133	38.0	33.0	40.0	24.0	41.0
26-27	34.28615703735173	38.0	33.0	40.0	23.0	41.0
28-29	34.224088952131	38.0	33.0	40.0	23.5	41.0
30-31	34.207206166788126	38.0	33.0	40.0	23.0	41.0
32-33	34.056017999261655	38.0	33.0	40.0	22.5	41.0
34-35	33.89006483240379	38.0	33.0	40.0	21.5	41.0
36-37	33.688209914181684	38.0	33.0	40.0	19.5	41.0
38-39	33.508902219068645	38.0	32.0	40.0	18.5	41.0
40-41	33.28158860183255	38.0	32.0	40.0	17.0	41.0
42-43	33.31453936874621	38.0	32.0	40.0	17.0	41.0
44-45	33.171448213733875	38.0	32.0	40.0	15.5	41.0
46-47	32.920510245669576	37.5	32.0	40.0	14.5	41.0
48-49	32.663122947395095	37.0	31.0	40.0	11.0	41.0
50-51	32.403115768676344	37.0	31.0	40.0	9.0	41.0
52-53	32.14265656100983	37.0	31.0	40.0	8.0	41.0
54-55	31.860317776670186	36.0	30.0	40.0	7.0	41.0
56-57	31.53220346357184	36.0	30.0	39.0	4.0	41.0
58-59	31.202533121597387	35.5	29.5	39.0	2.0	40.0
60-61	30.86947160279292	35.0	29.0	39.0	2.0	40.0
62-63	30.54059111182486	35.0	29.0	39.0	2.0	40.0
64-65	30.13359182905692	35.0	28.0	38.0	2.0	40.0
66-67	29.697067506188606	34.0	28.0	38.0	2.0	40.0
68-69	29.25955067106944	34.0	28.0	37.0	2.0	39.5
70-71	28.8354849761957	34.0	26.5	36.5	2.0	39.0
72-73	28.39282835120442	34.0	26.0	36.0	2.0	39.0
74-75	27.95468049902972	33.0	26.0	35.5	2.0	38.5
76-77	26.50080792576616	31.5	24.5	34.0	2.0	36.0
78-79	27.342462153372495	33.0	26.0	35.0	2.0	37.0
80-81	27.36882123134861	33.0	26.0	35.0	2.0	36.5
82-83	27.034787034542944	33.0	25.5	35.0	2.0	36.0
84-85	26.71571537979709	33.0	25.0	35.0	2.0	36.0
86-87	26.176757074588934	32.5	24.0	35.0	2.0	35.0
88-89	25.812654155307293	32.0	23.0	35.0	2.0	35.0
90-91	25.44656733119934	32.0	20.5	34.5	2.0	35.0
92-93	25.13936247603095	32.0	19.5	34.0	2.0	35.0
94-95	24.744259424307707	32.0	17.5	34.0	2.0	35.0
96-97	24.204291811717948	31.5	6.0	34.0	2.0	35.0
98-99	23.59573400479733	31.0	2.0	34.0	2.0	35.0
100-101	21.43625144198194	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	73303.0
3	32612.0
4	17804.0
5	12556.0
6	10685.0
7	10776.0
8	12310.0
9	14166.0
10	16052.0
11	17693.0
12	19587.0
13	21342.0
14	23216.0
15	25084.0
16	26712.0
17	27466.0
18	26684.0
19	25540.0
20	25776.0
21	27271.0
22	29513.0
23	33728.0
24	38889.0
25	46449.0
26	54718.0
27	64680.0
28	76304.0
29	89453.0
30	105714.0
31	125141.0
32	149824.0
33	183721.0
34	230408.0
35	299033.0
36	396151.0
37	460004.0
38	259078.0
39	16463.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.20361466982509	19.423874538118575	13.932769871918346	24.439740920137982
2	16.140920424350572	20.707308537108922	38.27623095512149	24.875540083419015
3	19.30757232784892	24.753409427216226	29.031602690747953	26.907415554186898
4	13.897116932065051	16.72019427353791	35.716159706158074	33.66652908823897
5	14.664128154957137	37.227470441972336	33.5522805014915	14.556120901579032
6	33.354416961357074	35.10613017960689	17.192358908895027	14.34709395014101
7	29.411745860371536	31.577984561192174	20.632577838791168	18.377691739645122
8	26.854379940806417	34.20008584350857	19.97847506053889	18.967059155146128
9	27.174874502905276	15.551291781506107	19.338069902898937	37.93576381268968
10-11	25.91447351774884	25.440938473753477	27.243782630600066	21.40080537789762
12-13	26.60841798129231	22.705873498075903	27.200011530164993	23.485696990466796
14-15	23.917538650754757	24.172583987521577	24.806081628600438	27.103795733123228
16-17	23.828041529846473	27.165363090040824	25.40950819960769	23.597087180505014
18-19	23.80476397518312	26.416008027257842	26.865604263104004	22.913623734455033
20-21	24.953791556596084	25.53050301316975	26.35024583963994	23.16545959059423
22-23	24.358140856569957	25.504508578907135	26.237263714844893	23.900086849678015
24-25	24.08200507642242	25.59633719109214	26.390354445294655	23.93130328719078
26-27	23.997420422201785	25.80719048507779	26.365026584889335	23.83036250783109
28-29	23.86029903003662	25.8942857531478	26.366569827682078	23.8788453891335
30-31	24.232200078205548	25.787335309164703	26.109293212564854	23.871171400064895
32-33	24.014085036640648	25.731016889787483	26.065574111488885	24.18932396208298
34-35	24.332953957512682	25.670773673899124	26.126952566930186	23.869319801658012
36-37	24.32946007862984	25.609430844828385	25.99974817204745	24.06136090449433
38-39	24.162565852370683	25.521606777844003	26.154957578472825	24.160869791312486
40-41	24.302801338119693	25.47622396049574	26.189249956361838	24.03172474502273
42-43	24.255557205073632	25.418110172734405	26.043803663551213	24.28252895864075
44-45	24.03478852442046	25.418044843072284	26.201167436013495	24.345999196493757
46-47	24.077493830437724	25.340880016474564	26.097420440528268	24.484205712559444
48-49	23.96713926699226	25.42742788219278	26.117884000428717	24.487548850386244
50-51	23.871664426953352	25.554952966020352	26.12900748704166	24.44437511998464
52-53	24.044173975860982	25.439675412105466	26.122325447545684	24.393825164487865
54-55	23.793255865926792	25.35136529945018	26.243354434581622	24.612024400041403
56-57	23.81302393643483	25.357475692932578	26.054911209740293	24.774589160892297
58-59	23.608356690147716	25.384935550548455	26.231091537704316	24.775616221599517
60-61	23.728051018432744	25.44801921190857	26.15144257306461	24.67248719659408
62-63	23.55158743489602	25.413355678007616	26.133686676092125	24.901370211004238
64-65	23.363972743663457	25.328486781265454	26.11640075203353	25.19113972303756
66-67	23.444432158390036	25.41667460103972	25.960185822750216	25.17870741782003
68-69	23.543204079270332	25.298033835631944	25.922927868134888	25.23583421696284
70-71	23.325742367063164	25.096937609872754	26.005085744747706	25.572234278316376
72-73	23.28166606923392	25.1468564369111	25.970433480131096	25.601044013723882
74-75	23.210688998063326	25.165758845314638	25.94782520712751	25.67572694949452
76-77	23.152864965405552	25.06469121549575	26.07359838372177	25.708845435376933
78-79	23.200629840788466	25.092313361800823	25.97870733438584	25.72834946302487
80-81	23.044368104904244	25.141579486168737	25.96929094190454	25.844761467022487
82-83	22.972306923453107	25.08243005942835	25.985877268436365	25.959385748682184
84-85	22.87551858125862	24.995244722041406	25.949755789080985	26.179480907618984
86-87	22.883697374088964	24.975984183399692	25.955059711579263	26.18525873093208
88-89	22.70197169532834	24.947069175927346	25.969727785712976	26.381231343031335
90-91	22.65603899507365	24.9267687605733	25.977667519266905	26.439524725086144
92-93	22.7330485076831	24.798548133993904	25.9231792603779	26.5452240979451
94-95	22.529492974999133	24.80826547092547	25.95828075466314	26.703960799412258
96-97	22.453394484934737	24.707309383391397	26.031585360918307	26.807710770755556
98-99	22.301846066385707	24.681550389283284	25.99821774110602	27.01838580322499
100-101	22.387412217376824	24.671000118051253	26.007170733782907	26.934416930789013
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	96.0
1	67.0
2	65.5
3	106.0
4	100.5
5	101.5
6	157.5
7	328.5
8	499.0
9	518.0
10	472.0
11	454.5
12	465.5
13	486.5
14	532.0
15	595.0
16	661.0
17	739.0
18	835.0
19	913.0
20	1037.0
21	1236.5
22	1469.5
23	1687.0
24	1986.5
25	2493.5
26	3168.0
27	3976.0
28	4930.0
29	6512.5
30	8905.5
31	12696.0
32	18400.5
33	25127.0
34	31945.5
35	39316.5
36	48219.0
37	57377.0
38	65577.0
39	73553.0
40	82266.5
41	90637.5
42	97868.5
43	106135.0
44	114476.5
45	121324.0
46	128575.0
47	136265.0
48	143237.5
49	147128.5
50	147106.0
51	144252.5
52	139132.0
53	135063.0
54	131307.5
55	124375.5
56	114007.5
57	103477.0
58	95204.0
59	84420.5
60	70620.5
61	57791.0
62	46998.5
63	36234.5
64	26625.0
65	20312.5
66	15385.5
67	11848.5
68	9114.0
69	6577.0
70	5050.0
71	4009.0
72	3092.5
73	2551.5
74	1901.0
75	1351.0
76	982.0
77	617.5
78	354.0
79	201.0
80	110.0
81	59.0
82	42.5
83	24.5
84	15.0
85	9.0
86	4.5
87	2.0
88	0.5
89	1.5
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.5
97	0.5
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18513032701559165
2	0.0
3	0.01218846631984455
4	0.013308141703557305
5	0.006078237797297807
6	0.10940828035136053
7	0.14558979060790694
8	0.12623540183230078
9	0.144566087399941
10-11	0.1166061935323711
12-13	0.11711804513635407
14-15	0.11887753502504554
16-17	0.06809225869235991
18-19	0.0815123679342885
20-21	0.03859681001284108
22-23	0.08616701845800864
24-25	0.059358790699400435
26-27	0.06842816130747374
28-29	0.04169991036198785
30-31	0.025848506001140147
32-33	0.032934451643779436
34-35	0.019098462973614692
36-37	0.024200983650819956
38-39	0.03258255366604114
40-41	0.024776816705300797
42-43	0.013244160253059434
44-45	0.027272093274717794
46-47	0.034853895158715585
48-49	0.009309301047440325
50-51	0.01618730697596153
52-53	0.007021964192141415
54-55	0.013420109241928579
56-57	0.005406432567070155
58-59	0.003934859205619107
60-61	0.024344941914440164
62-63	0.010524948606899887
64-65	0.007917704499111618
66-67	0.042083799064975086
68-69	0.09266113568354263
70-71	0.02239350767425508
72-73	0.04561877420498249
74-75	0.08859831357692778
76-77	0.2441212243746293
78-79	0.08244009896650763
80-81	0.01732297772229875
82-83	0.012076498781473275
84-85	0.01380399794491581
86-87	0.2613322345585568
88-89	0.6235952072775062
90-91	0.268210240487078
92-93	0.35247381079277496
94-95	0.0011036800210882862
96-97	0.003918863842994639
98-99	0.0031190957117712436
100-101	0.004638655161095695
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3125906.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.93189014017179
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.86220551586779	34.18489765252546
2	13.319772331299212	13.035232728144658
3	6.050588219203428	8.882001540764396
4	3.277532579718873	6.415034564865508
5	2.0371732242745506	4.984136820335093
6	1.3340252332273268	3.916582569389796
7	0.8943012333256438	3.063189479091738
8	0.6641719901086715	2.5999352683341423
9	0.492457891766119	2.168720591270431
>10	1.9733133841769284	15.709420918681685
>50	0.06647319282848518	2.2289827722738504
>100	0.02693442349958024	2.288346825336232
>500	6.567379397993064E-4	0.2004929397696487
>1k	3.940427635287644E-4	0.32302532921733146
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	3.199072524893583E-5	0.0	0.0	0.0
6	0.0	3.199072524893583E-5	0.0	0.0	0.0
7	0.0	3.199072524893583E-5	0.0	3.199072524893583E-5	0.0
8	0.0	3.199072524893583E-5	0.0	3.199072524893583E-5	0.0
9	0.0	3.199072524893583E-5	0.0	3.199072524893583E-5	0.0
10-11	0.0	4.798608787340374E-5	0.0	3.199072524893583E-5	0.0
12-13	3.199072524893583E-5	7.997681312233957E-5	0.0	9.597217574680748E-5	0.0
14-15	3.199072524893583E-5	9.597217574680748E-5	0.0	1.5995362624467914E-4	0.0
16-17	3.199072524893583E-5	1.119675383712754E-4	0.0	1.9194435149361497E-4	0.0
18-19	3.199072524893583E-5	1.5995362624467914E-4	0.0	1.9194435149361497E-4	0.0
20-21	3.199072524893583E-5	2.559258019914866E-4	0.0	2.079397141180829E-4	0.0
22-23	3.199072524893583E-5	3.199072524893583E-4	0.0	3.5189797773829413E-4	0.0
24-25	3.199072524893583E-5	3.199072524893583E-4	0.0	0.00115166610896169	0.0
26-27	3.199072524893583E-5	3.199072524893583E-4	0.0	0.0025432626572903985	0.0
28-29	3.199072524893583E-5	3.6789334036276206E-4	0.0	0.00820562102635204	0.0
30-31	3.199072524893583E-5	4.638655161095695E-4	0.0	0.029959314195628403	0.0
32-33	3.199072524893583E-5	5.438423292319091E-4	0.0	0.07746554119029811	0.0
34-35	3.199072524893583E-5	5.75833054480845E-4	0.0	0.14573374887152718	3.199072524893583E-5
36-37	3.199072524893583E-5	5.918284171053128E-4	0.0	0.2589329301648866	3.199072524893583E-5
38-39	3.199072524893583E-5	6.078237797297807E-4	0.0	0.44745427405686544	3.199072524893583E-5
40-41	3.199072524893583E-5	6.078237797297807E-4	0.0	0.6623999570044653	3.199072524893583E-5
42-43	3.199072524893583E-5	6.078237797297807E-4	0.0	0.8833279055736161	3.199072524893583E-5
44-45	3.199072524893583E-5	6.078237797297807E-4	0.0	1.1550091397502036	3.199072524893583E-5
46-47	3.199072524893583E-5	6.078237797297807E-4	0.0	1.474644471074946	3.199072524893583E-5
48-49	3.199072524893583E-5	6.078237797297807E-4	0.0	1.8172811338536732	3.199072524893583E-5
50-51	3.199072524893583E-5	6.238191423542487E-4	0.0	2.1826632022843935	6.398145049787166E-5
52-53	3.199072524893583E-5	6.398145049787166E-4	0.0	2.5541075131497877	6.398145049787166E-5
54-55	3.199072524893583E-5	6.398145049787166E-4	0.0	2.9713305518464086	6.398145049787166E-5
56-57	3.199072524893583E-5	6.718052302276524E-4	0.0	3.462084272527709	6.398145049787166E-5
58-59	3.199072524893583E-5	7.197913181010561E-4	0.0	3.9886836008504414	6.398145049787166E-5
60-61	3.199072524893583E-5	7.997681312233957E-4	0.0	4.538700140055395	6.398145049787166E-5
62-63	3.199072524893583E-5	8.317588564723316E-4	0.0	5.098121952483536	6.398145049787166E-5
64-65	3.199072524893583E-5	8.317588564723316E-4	0.0	5.6959486305730245	6.398145049787166E-5
66-67	3.199072524893583E-5	8.637495817212673E-4	0.0	6.369641313590364	6.398145049787166E-5
68-69	3.199072524893583E-5	8.957403069702032E-4	0.0	7.112641902859523	6.398145049787166E-5
70-71	3.199072524893583E-5	8.957403069702032E-4	0.0	7.900221567763074	6.398145049787166E-5
72-73	3.199072524893583E-5	0.0010077078453414787	0.0	8.699157940129998	6.398145049787166E-5
74-75	3.199072524893583E-5	0.0010556939332148824	0.0	9.52035985727018	9.597217574680748E-5
76-77	3.199072524893583E-5	0.001119675383712754	0.0	10.404103642272032	1.279629009957433E-4
78-79	3.199072524893583E-5	0.001119675383712754	0.0	11.362705724356395	1.279629009957433E-4
80-81	3.199072524893583E-5	0.0011676614715861577	0.0	12.369597806204025	1.279629009957433E-4
82-83	3.199072524893583E-5	0.0011996521968350936	0.0	13.397203882650341	1.279629009957433E-4
84-85	3.199072524893583E-5	0.0012156475594595614	0.0	14.419883387408323	1.279629009957433E-4
86-87	3.199072524893583E-5	0.0012476382847084974	0.0	15.478216555456243	1.279629009957433E-4
88-89	3.199072524893583E-5	0.001279629009957433	0.0	16.602354645341222	1.279629009957433E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3765	0.0	44.462887	1
GTATCAA	6900	0.0	41.69906	1
ATCAACG	9260	0.0	30.656055	3
TCAACGC	9320	0.0	30.356829	4
TATCAAC	9420	0.0	30.233725	2
CAACGCA	9450	0.0	29.734474	5
AACGCAG	10020	0.0	28.185102	6
GTACATG	13715	0.0	24.168903	1
TACATGG	13975	0.0	23.436274	2
ACGCAGA	11670	0.0	23.345943	7
CGCAGAG	11850	0.0	22.868593	8
ACATGGG	14340	0.0	22.609846	3
GCAGAGT	13125	0.0	19.851564	9
AGAGTAC	10970	0.0	19.29353	10-11
CATGGGG	8560	0.0	18.799747	4
GAGTACA	10725	0.0	17.20499	1
AGTACAT	10325	0.0	16.73411	2
CATGGGA	5590	0.0	16.474642	4
CAGAGTA	12280	0.0	16.269236	10-11
GTGGTAT	2200	0.0	16.21282	1
>>END_MODULE