FastQCFastQC Report
Mon 15 Aug 2016
SRR2920886_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920886_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4847211
Sequences flagged as poor quality0
Sequence length98
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41900.045.039891
GTATCAA80000.043.094251
TCAACGC101250.033.785644
ATCAACG102150.033.4879723
CAACGCA102000.033.312445
TATCAAC106100.032.2419132
AACGCAG110650.030.8278086
GTACATG157350.026.999921
ACGCAGA129500.025.8767977
TACATGG163850.025.5238252
CGCAGAG135000.024.5830828
ACATGGG167550.024.2754673
CATGGGG114250.020.8706284
GCAGAGT153300.020.5718489
GAGTACA115850.018.6339231
AGAGTAC128650.018.10047310-11
TATACCG2856.405753E-817.698495
TAAGGCG10450.017.552225
GTATTAA21850.017.4845391
AGTACAT114550.017.3336542
Produced by FastQC (version 0.11.5)