##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920886_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4847211
Sequences flagged as poor quality	0
Sequence length	98
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.452868876556025	31.0	28.0	33.0	20.0	34.0
2	28.4814160142812	31.0	28.0	33.0	16.0	34.0
3	28.5532633095609	31.0	28.0	34.0	18.0	34.0
4	32.01200814241427	35.0	32.0	37.0	22.0	37.0
5	31.970749777552495	35.0	32.0	37.0	22.0	37.0
6	32.09434951356564	35.0	32.0	37.0	22.0	37.0
7	31.92743971739625	35.0	32.0	37.0	20.0	37.0
8	31.864703228310052	35.0	32.0	37.0	19.0	37.0
9	33.11258989963507	37.0	32.0	39.0	19.0	39.0
10-11	32.916966684553245	37.0	32.0	39.0	18.0	39.0
12-13	32.739966137228194	37.0	32.0	39.0	17.0	39.0
14-15	33.47833207590922	38.0	32.0	40.0	17.0	41.0
16-17	33.35730526275832	38.0	32.0	40.0	17.0	41.0
18-19	33.23977953507698	38.0	32.0	40.0	16.5	41.0
20-21	33.14746057062504	38.0	32.0	40.0	15.5	41.0
22-23	32.9169314065346	38.0	32.0	40.0	11.0	41.0
24-25	32.71687553935655	38.0	31.0	40.0	10.0	41.0
26-27	32.751577350356726	38.0	31.5	40.0	10.0	41.0
28-29	32.588433843709296	38.0	31.0	40.0	9.5	41.0
30-31	32.34023235217117	37.0	31.0	40.0	8.5	41.0
32-33	32.08993780134597	37.0	30.0	40.0	8.0	41.0
34-35	31.757553261040215	37.0	30.0	40.0	5.0	41.0
36-37	31.44932921220058	37.0	30.0	40.0	2.0	41.0
38-39	31.24899225554654	37.0	30.0	40.0	2.0	41.0
40-41	31.051432256611072	36.0	29.5	40.0	2.0	41.0
42-43	30.81839329874437	36.0	29.0	39.5	2.0	41.0
44-45	30.533788502295444	36.0	28.0	39.0	2.0	40.0
46-47	30.281599975738626	36.0	28.0	39.0	2.0	40.0
48-49	29.97676447342606	35.0	27.0	39.0	2.0	40.0
50-51	28.859033369911067	34.0	25.5	38.0	2.0	39.5
52-53	29.320617980112686	34.5	27.0	38.5	2.0	39.5
54-55	29.96787998294277	35.0	28.0	39.0	2.0	40.0
56-57	29.916796689890333	35.0	28.0	39.0	2.0	40.0
58-59	29.66909713647704	35.0	27.5	39.0	2.0	40.0
60-61	29.34116742596928	35.0	27.0	39.0	2.0	40.0
62-63	28.947361998477064	34.5	26.0	38.5	2.0	40.0
64-65	28.519602509566845	34.0	26.0	38.0	2.0	40.0
66-67	28.13378187993054	34.0	25.5	37.5	2.0	40.0
68-69	27.683928655055453	34.0	24.5	37.0	2.0	39.5
70-71	27.351293042535183	33.5	24.0	36.5	2.0	39.0
72-73	26.887072896145845	33.0	22.5	36.0	2.0	39.0
74-75	26.445607587538483	33.0	22.0	35.5	2.0	38.5
76-77	26.023147950439956	32.5	20.5	35.0	2.0	37.0
78-79	25.558830634771212	32.0	19.0	35.0	2.0	37.0
80-81	25.105767316504274	32.0	17.5	35.0	2.0	36.0
82-83	24.629289915376077	31.5	11.5	35.0	2.0	36.0
84-85	24.222500320287274	31.0	7.0	34.0	2.0	35.0
86-87	23.754505941664185	31.0	2.0	34.0	2.0	35.0
88-89	23.214062891010933	31.0	2.0	34.0	2.0	35.0
90-91	22.707142519688126	30.0	2.0	34.0	2.0	35.0
92-93	22.161094287003394	30.0	2.0	34.0	2.0	35.0
94-95	21.515525525915834	29.5	2.0	34.0	2.0	35.0
96-97	20.861745548110036	29.0	2.0	34.0	2.0	35.0
98	20.45802565640324	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	258410.0
3	44343.0
4	32902.0
5	26720.0
6	24479.0
7	24925.0
8	28928.0
9	33284.0
10	35850.0
11	38116.0
12	39579.0
13	39434.0
14	37275.0
15	39024.0
16	42048.0
17	44719.0
18	46436.0
19	45941.0
20	48144.0
21	50154.0
22	55046.0
23	61985.0
24	70552.0
25	80647.0
26	92630.0
27	107089.0
28	123506.0
29	142177.0
30	165442.0
31	193622.0
32	229281.0
33	276563.0
34	340556.0
35	428447.0
36	539352.0
37	585390.0
38	341316.0
39	32898.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.23126706764015	17.795369323747636	12.979669807823498	24.99369380078872
2	16.075183113617626	19.362158960882454	39.22497464421945	25.33768328128047
3	19.60476497338887	23.22331288672696	28.74364899600333	28.428273143880844
4	13.49845814500762	15.324480778138982	36.082398711630304	35.094662365223094
5	14.77456121145814	36.45298606495455	33.89587189935386	14.876580824233454
6	34.01811360935024	34.430488147120975	17.108116250660327	14.443281992868462
7	29.85750238266349	31.108000941716924	20.514325177495277	18.520171498124306
8	26.960189108594456	34.10748959248682	19.813817084022887	19.11850421489583
9	27.0089335908835	15.696283904290528	19.265800477841793	38.028982026984174
10-11	25.792386647363458	25.10786880899982	27.418972676535923	21.680771867100802
12-13	26.465299294692223	22.428100323460345	27.271133959787598	23.835466422059838
14-15	23.644277561293883	23.981638268736315	24.84065782449589	27.53342634547391
16-17	23.80010986375347	26.89994919446142	25.562561733887296	23.73737920789781
18-19	23.70872981926794	26.278686164754127	26.934055938247	23.078528077730937
20-21	24.56761317932785	25.598249517287623	26.592919860661706	23.24121744272281
22-23	24.216738105472402	25.34386496862822	26.464249432032844	23.975147493866533
24-25	23.887918749701957	25.490788471649907	26.588599764703112	24.032693013945025
26-27	23.78136002584025	25.801765688438977	26.463276899145292	23.953597386575478
28-29	23.832181020043937	25.94184942876995	26.36322783683954	23.86274171434658
30-31	24.103231181132205	25.667311839187533	26.23106203295602	23.998394946724243
32-33	23.844028799305356	25.747044918233314	26.15944813161907	24.249478150842265
34-35	24.21257566685418	25.757051501330785	26.166538754729423	23.863834077085617
36-37	24.270125424129866	25.46662191418078	26.144068372176022	24.119184289513328
38-39	24.039214930303363	25.647570981789	26.170710331950346	24.142503755957296
40-41	24.217684268526614	25.491562224712354	26.29480746150728	23.995946045253753
42-43	24.041726296430667	25.39849810693845	26.227371682998335	24.33240391363255
44-45	24.03466641698881	25.49489865882391	26.24875868814725	24.221676236040032
46-47	24.009229443199807	25.501484331686168	26.12483838997629	24.364447835137735
48-49	23.878694422495187	25.5468092223473	26.139698077176003	24.434798277981507
50-51	23.883495366820195	25.668637316687764	26.1950679451228	24.252799371369235
52-53	23.936123639473706	25.48238383904951	26.31952956287732	24.261962958599465
54-55	23.660069156250227	25.416921831884203	26.4775801736885	24.44542883817707
56-57	23.626411647332276	25.51694018234747	26.415407650621468	24.441240519698784
58-59	23.58636900250217	25.301564462426946	26.47230636558866	24.639760169482223
60-61	23.526434470722197	25.24432700578943	26.67661198227868	24.552626541209698
62-63	23.466773026511007	25.26821354507532	26.69326905619344	24.571744372220238
64-65	23.40181058640137	25.163996835079715	26.56180899482281	24.8723835836961
66-67	23.341311197756482	25.220595223375884	26.556121441661908	24.881972137205725
68-69	23.50759436700833	24.99425379329918	26.554187065829932	24.943964773862554
70-71	23.310583339226444	24.933271527290206	26.64348146248394	25.112663670999407
72-73	23.291848979213654	24.918181286437708	26.678506521841534	25.11146321250711
74-75	23.32226428749461	25.061845473056284	26.617898276565917	24.99799196288319
76-77	23.291856934162116	24.849136739838272	26.74660540845124	25.11240091754837
78-79	23.2673529673569	24.862287894376212	26.7505635794176	25.119795558849283
80-81	23.293442425922592	24.85441663184067	26.74391292109911	25.10822802113763
82-83	23.293439629130987	24.750945567329012	26.81675320263121	25.13886160090879
84-85	23.284401663969366	24.738428744360665	26.799105284177237	25.178064307492726
86-87	23.25220366040921	24.669187008598527	26.93243350102032	25.146175829971934
88-89	23.262280512556124	24.519368614650684	26.889150123576798	25.32920074921639
90-91	23.27084197540092	24.52303292393528	26.946345454882376	25.25977964578143
92-93	23.368063092620318	24.309278800851832	27.031389694481422	25.291268412046424
94-95	23.30204105801597	24.213386650699615	27.0659961829801	25.418576108304315
96-97	23.464781176820367	24.004788052684315	27.08983768633907	25.440593084156248
98	23.48867888512597	23.95226428508432	26.975776048004857	25.583280781784858
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	107.0
1	94.0
2	138.0
3	219.5
4	235.0
5	264.0
6	398.0
7	801.0
8	1279.0
9	1449.0
10	1457.0
11	1593.5
12	1809.5
13	2003.0
14	2282.0
15	2575.0
16	2749.5
17	2879.5
18	2935.5
19	2896.5
20	2949.5
21	3053.0
22	3193.5
23	3541.0
24	4141.5
25	5083.5
26	6450.0
27	8267.5
28	10651.5
29	14398.5
30	20072.0
31	28233.5
32	37866.5
33	48516.5
34	61032.0
35	75033.5
36	90011.0
37	104367.0
38	116945.0
39	129487.5
40	142473.0
41	153624.0
42	165639.0
43	178322.5
44	189702.5
45	200448.5
46	209328.0
47	216925.5
48	223516.0
49	225258.0
50	224054.0
51	222882.5
52	219570.0
53	215187.0
54	206818.0
55	194477.5
56	178688.0
57	160192.0
58	143320.0
59	125101.0
60	105749.0
61	87521.0
62	70784.0
63	54721.0
64	39973.5
65	29567.5
66	22423.5
67	16791.5
68	12308.0
69	9197.0
70	6821.0
71	5044.0
72	3857.5
73	2968.5
74	2326.5
75	1692.5
76	1080.5
77	613.5
78	322.5
79	175.5
80	106.0
81	74.0
82	55.0
83	34.5
84	21.5
85	17.0
86	11.5
87	11.0
88	12.0
89	10.5
90	8.0
91	7.0
92	6.5
93	5.0
94	4.0
95	3.5
96	1.5
97	1.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3843240989509225
2	0.024261374221175847
3	0.019186290838174778
4	0.016009206118735082
5	0.022363375557614473
6	0.02333300530965126
7	0.015349032670539822
8	0.009737558360880102
9	0.0
10-11	7.220647089635669E-5
12-13	0.005136974643769375
14-15	0.003579377914433682
16-17	0.006158180446446421
18-19	0.03669120242547725
20-21	0.07730218469961386
22-23	0.09740652923918516
24-25	0.06432565035852576
26-27	0.07436234981311934
28-29	0.05679554696504856
30-31	0.051823615683328
32-33	0.020279703111748176
34-35	0.3386174028735287
36-37	0.5184940370864812
38-39	0.4719208633583312
40-41	0.4802658683519244
42-43	0.4312170441930422
44-45	0.40553217097419525
46-47	0.4047172693740792
48-49	0.3951963304258882
50-51	0.3907298444404421
52-53	0.45028986771980833
54-55	0.45192998613016844
56-57	0.4227585718880404
58-59	0.40852358191133004
60-61	0.4225728981057354
62-63	0.4038301613030669
64-65	0.450011357046351
66-67	0.4707965054543736
68-69	0.41494995782110583
70-71	0.3739366823519752
72-73	0.3605888004462772
74-75	0.34293947591718205
76-77	0.34064949926875476
78-79	0.3400099562408156
80-81	0.3558025429468616
82-83	0.3515836220044888
84-85	0.352243795452684
86-87	0.3380294358962298
88-89	0.38027022137059846
90-91	0.3588971059852769
92-93	0.3489738738420919
94-95	0.3561842057215995
96-97	0.3563389338735203
98	0.35139794822218384
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	4847211.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.37876855974698
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.458150656059	40.48942541912606
2	12.557010821342654	13.656695705120612
3	5.0379089338714795	8.218658518202366
4	2.5659648140288813	5.5813602701812295
5	1.4850444854109135	4.0377445186544305
6	0.9351342062467025	3.0510867944275275
7	0.6145519687577011	2.339300549390832
8	0.4399766178540038	1.914030933918649
9	0.31214095797622243	1.5276456820626039
>10	1.501384615487283	14.068882954127956
>50	0.06941579257836104	2.5356726614938148
>100	0.022092435814195	1.9316380620974314
>500	8.412907879283586E-4	0.3000882563758949
>1k	3.824037846876696E-4	0.3477696748205775
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2378252153661147E-4
2	0.0	0.0	0.0	2.0630420256101912E-5	1.2378252153661147E-4
3	0.0	0.0	0.0	2.0630420256101912E-5	1.2378252153661147E-4
4	0.0	0.0	0.0	2.0630420256101912E-5	1.2378252153661147E-4
5	0.0	2.0630420256101912E-5	0.0	2.0630420256101912E-5	1.4441294179271338E-4
6	0.0	2.0630420256101912E-5	0.0	2.0630420256101912E-5	2.4756504307322293E-4
7	0.0	2.0630420256101912E-5	0.0	2.0630420256101912E-5	2.4756504307322293E-4
8	0.0	2.0630420256101912E-5	0.0	2.0630420256101912E-5	2.4756504307322293E-4
9	0.0	2.0630420256101912E-5	0.0	2.0630420256101912E-5	2.4756504307322293E-4
10-11	0.0	4.1260840512203824E-5	0.0	2.0630420256101912E-5	2.4756504307322293E-4
12-13	0.0	8.252168102440765E-5	0.0	2.0630420256101912E-5	7.736407596038216E-4
14-15	0.0	9.28368911524586E-5	0.0	4.1260840512203824E-5	9.593145419087389E-4
16-17	0.0	1.0315210128050955E-4	0.0	5.1576050640254775E-5	0.001155303534341707
18-19	0.0	1.0315210128050955E-4	0.0	8.252168102440763E-5	0.0012378252153661146
20-21	0.0	1.340977316646624E-4	0.0	1.4441294179271338E-4	0.0014234989976710318
22-23	0.0	1.4441294179271338E-4	0.0	2.681954633293248E-4	0.0015060206786954395
24-25	0.0	1.4441294179271338E-4	0.0	8.045863899879746E-4	0.0015060206786954395
26-27	0.0	1.4441294179271338E-4	0.0	0.0017329553015125606	0.001640118410360102
28-29	0.0	1.650433620488153E-4	0.0	0.0069833972566904975	0.0016916944610003567
30-31	0.0	1.650433620488153E-4	0.0	0.029780011639683107	0.0017123248812564587
32-33	0.0	1.650433620488153E-4	0.0	0.08088156261404754	0.0017845313521528154
34-35	0.0	1.9598899243296816E-4	0.0	0.14858028668444595	0.0018773682433052739
36-37	0.0	2.4756504307322293E-4	0.0	0.26158341363724413	0.0018773682433052739
38-39	0.0	2.991410937134777E-4	0.0	0.4475047609852346	0.0020630420256101912
40-41	0.0	3.0945630384152865E-4	0.0	0.6616072624030602	0.0021249332863784967
42-43	0.0	3.0945630384152865E-4	0.0	0.8887585046328703	0.0021558789167626497
44-45	0.0	3.0945630384152865E-4	0.0	1.163988941269526	0.002248715807915108
46-47	0.0	3.0945630384152865E-4	0.0	1.4873295179434112	0.0023828135395797707
48-49	0.0	3.0945630384152865E-4	0.0	1.8353543924537223	0.002465335220604178
50-51	0.0	3.0945630384152865E-4	0.0	2.2079088366485387	0.0026819546332932484
52-53	0.0	3.3008672409763054E-4	0.0	2.59239797896151	0.0028882588358542676
54-55	0.0	3.8166277473788534E-4	0.0	3.0201903733920394	0.0028882588358542676
56-57	0.0	3.919779848659363E-4	0.0	3.511039647335344	0.0029604653067506243
58-59	0.0	4.126084051220382E-4	0.0	4.039395025304242	0.003042986987775032
60-61	0.0	4.5386924563424203E-4	0.0	4.600841597363927	0.0030945630384152864
62-63	0.0	4.5386924563424203E-4	0.0	5.170292772482981	0.003321497661232408
64-65	0.0	4.5386924563424203E-4	0.0	5.780561234078731	0.003424649762512917
66-67	1.0315210128050956E-5	4.5386924563424203E-4	0.0	6.469493488110999	0.0034555953928970705
68-69	2.0630420256101912E-5	4.5386924563424203E-4	0.0	7.219976600977345	0.003496856233409274
70-71	2.0630420256101912E-5	4.744996658903439E-4	0.0	8.006604210132384	0.0036000083346897833
72-73	2.0630420256101912E-5	4.951300861464459E-4	0.0	8.814347054419542	0.0038269429575069043
74-75	2.0630420256101912E-5	4.951300861464459E-4	0.0	9.645526056117632	0.004053877580324026
76-77	2.0630420256101912E-5	4.951300861464459E-4	0.0	10.551407809563067	0.004167344891732586
78-79	2.0630420256101912E-5	5.157605064025478E-4	0.0	11.525184276071332	0.004239551362628943
80-81	2.0630420256101912E-5	5.157605064025478E-4	0.0	12.525532723869457	0.004332388253781402
82-83	2.0630420256101912E-5	5.157605064025478E-4	0.0	13.544025626282824	0.004497431615830216
84-85	2.0630420256101912E-5	5.363909266586496E-4	0.0	14.575907671442401	0.004518062036086319
86	2.0630420256101912E-5	5.570213469147516E-4	0.0	15.364588007412923	0.004518062036086319
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4190	0.0	45.03989	1
GTATCAA	8000	0.0	43.09425	1
TCAACGC	10125	0.0	33.78564	4
ATCAACG	10215	0.0	33.487972	3
CAACGCA	10200	0.0	33.31244	5
TATCAAC	10610	0.0	32.241913	2
AACGCAG	11065	0.0	30.827808	6
GTACATG	15735	0.0	26.99992	1
ACGCAGA	12950	0.0	25.876797	7
TACATGG	16385	0.0	25.523825	2
CGCAGAG	13500	0.0	24.583082	8
ACATGGG	16755	0.0	24.275467	3
CATGGGG	11425	0.0	20.870628	4
GCAGAGT	15330	0.0	20.571848	9
GAGTACA	11585	0.0	18.633923	1
AGAGTAC	12865	0.0	18.100473	10-11
TATACCG	285	6.405753E-8	17.69849	5
TAAGGCG	1045	0.0	17.55222	5
GTATTAA	2185	0.0	17.484539	1
AGTACAT	11455	0.0	17.333654	2
>>END_MODULE