FastQCFastQC Report
Mon 15 Aug 2016
SRR2920886_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920886_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4847211
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43550.049.357741
GTATCAA81550.042.4533841
TCAACGC105800.031.9883164
ATCAACG108200.031.4981253
TATCAAC110850.031.0004482
CAACGCA108900.030.9428045
AACGCAG118050.028.7055516
GTACATG164700.024.3987791
TACATGG168000.023.3365062
ACGCAGA142850.023.256167
CGCAGAG148100.022.172878
ACATGGG171700.021.6460653
GCAGAGT164100.018.8833899
CATGGGG116700.018.099924
AGAGTAC141000.017.98766510-11
GTGGTAT27150.017.3408471
GAGTACA130150.016.8811781
GTATAGC15150.016.3228381
CAGAGTA152600.016.0606110-11
AGTACAT127900.015.84612
Produced by FastQC (version 0.11.5)