##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920886_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4847211
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.318077962770758	31.0	30.0	34.0	27.0	34.0
2	30.327433652052697	31.0	30.0	34.0	26.0	34.0
3	30.469581786309693	31.0	30.0	34.0	27.0	34.0
4	34.11712405339895	35.0	35.0	37.0	30.0	37.0
5	33.65151919320203	35.0	35.0	37.0	28.0	37.0
6	33.59873915123563	35.0	35.0	37.0	28.0	37.0
7	33.417949208317935	35.0	35.0	37.0	28.0	37.0
8	33.35076830779597	35.0	35.0	37.0	27.0	37.0
9	34.68894834576007	38.0	35.0	39.0	27.0	39.0
10-11	34.50604079335518	38.0	34.0	39.0	27.0	39.0
12-13	34.327104493697504	37.0	34.0	39.0	27.0	39.0
14-15	35.235100040002386	38.0	34.0	40.0	27.0	41.0
16-17	35.117525418225036	38.0	34.0	40.0	26.0	41.0
18-19	35.02983138138612	38.5	34.0	40.0	25.5	41.0
20-21	34.91449546966287	38.0	34.0	40.0	25.0	41.0
22-23	34.76840785350586	38.0	34.0	40.0	25.0	41.0
24-25	34.595177412330514	38.0	33.5	40.0	24.0	41.0
26-27	34.41470177386543	38.0	33.0	40.0	24.0	41.0
28-29	34.36237034038749	38.0	33.0	40.0	23.5	41.0
30-31	34.3545810157635	38.0	33.0	40.0	24.0	41.0
32-33	34.209419705475995	38.0	33.0	40.0	23.0	41.0
34-35	34.04364427296439	38.0	33.0	40.0	22.5	41.0
36-37	33.85620081320991	38.0	33.0	40.0	21.0	41.0
38-39	33.68241716731539	38.0	33.0	40.0	19.5	41.0
40-41	33.45450816974957	38.0	32.0	40.0	18.5	41.0
42-43	33.49078335975059	38.0	32.0	40.0	18.0	41.0
44-45	33.34884689360541	38.0	32.0	40.0	17.0	41.0
46-47	33.10513612879654	38.0	32.0	40.0	15.0	41.0
48-49	32.851342865000106	37.0	31.5	40.0	13.5	41.0
50-51	32.6006942961633	37.0	31.0	40.0	10.5	41.0
52-53	32.343169814559346	37.0	31.0	40.0	9.0	41.0
54-55	32.067467353907226	36.5	31.0	40.0	8.0	41.0
56-57	31.74063951414535	36.0	30.0	39.0	7.0	41.0
58-59	31.41277282957148	36.0	30.0	39.0	4.0	40.5
60-61	31.08480557169886	35.0	29.0	39.0	2.0	40.0
62-63	30.76806054038085	35.0	29.0	39.0	2.0	40.0
64-65	30.36537320120787	35.0	29.0	38.0	2.0	40.0
66-67	29.934465200710264	34.5	28.0	38.0	2.0	40.0
68-69	29.491597745590195	34.0	28.0	37.0	2.0	39.5
70-71	29.06229190352968	34.0	27.5	36.5	2.0	39.0
72-73	28.61589901904415	34.0	26.5	36.0	2.0	39.0
74-75	28.168097489463527	33.5	26.0	35.5	2.0	38.5
76-77	26.699223223416517	31.5	25.0	34.5	2.0	36.0
78-79	27.54499267723233	33.0	26.0	35.0	2.0	37.0
80-81	27.575012930115896	33.0	26.0	35.0	2.0	36.5
82-83	27.245779480200056	33.0	26.0	35.0	2.0	36.0
84-85	26.925288996084554	33.0	25.5	35.0	2.0	36.0
86-87	26.387495716608996	33.0	24.5	35.0	2.0	35.0
88-89	26.022348934263434	32.0	23.5	35.0	2.0	35.0
90-91	25.662350060684382	32.0	22.0	35.0	2.0	35.0
92-93	25.362951189869804	32.0	20.0	34.0	2.0	35.0
94-95	24.98871485066361	32.0	19.0	34.0	2.0	35.0
96-97	24.46191191181898	32.0	12.0	34.0	2.0	35.0
98-99	23.866634441950225	31.5	2.0	34.0	2.0	35.0
100-101	21.658814935021397	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	106681.0
3	49260.0
4	26673.0
5	18929.0
6	16224.0
7	16067.0
8	18136.0
9	21544.0
10	24207.0
11	26823.0
12	29862.0
13	32617.0
14	35108.0
15	37742.0
16	39998.0
17	41113.0
18	40338.0
19	39237.0
20	39142.0
21	41242.0
22	44480.0
23	50525.0
24	58645.0
25	69827.0
26	83089.0
27	98268.0
28	117019.0
29	136674.0
30	161849.0
31	192679.0
32	230998.0
33	281129.0
34	354971.0
35	462142.0
36	621547.0
37	734714.0
38	419413.0
39	28299.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.413418611448385	19.075996657997468	13.693342742533929	24.817241988020218
2	16.10552955091082	20.444911517159042	38.19951308082112	25.25004585110902
3	19.318684742363548	24.091378486812037	29.11645248114421	27.4734842896802
4	13.900695113161962	16.273909538519057	35.80359963347116	34.021795714847826
5	14.722336843495196	36.77483246954742	33.93030155481465	14.572529132142739
6	33.86383875465913	35.08865552882709	16.83926352798118	14.208242188532608
7	29.643182158591447	31.33883904822407	20.65899172076832	18.35898707241616
8	26.835436239947203	34.56209728142044	19.776011269665368	18.826455208966987
9	26.804487698618633	15.800821083737981	19.28094434546338	38.11374687218
10-11	25.787380595858934	25.310362988588835	27.49359219290546	21.40866422264677
12-13	26.420942047641134	22.561406628558636	27.401401328834556	23.616249994965674
14-15	23.561838068884466	24.14643125830359	24.880632304123125	27.411098368688826
16-17	23.747343791948786	27.116840537097215	25.594796035942043	23.541019635011956
18-19	23.700116662384264	26.43434806518104	26.937438467328185	22.92809680510651
20-21	24.546616358639206	25.721349731425097	26.577188133483094	23.154845776452603
22-23	24.17931902526287	25.475560338401987	26.52049229915383	23.824628337181313
24-25	23.89436465484303	25.655700295408568	26.58973300936303	23.86020204038537
26-27	23.785982616971083	25.874278825187584	26.49250607827988	23.847232479561445
28-29	23.832692423783534	26.00925324449173	26.399887314118605	23.758167017606137
30-31	24.084736791603085	25.774561422514967	26.260594936303995	23.880106849577956
32-33	23.859044468121592	25.84278647992123	26.158527663777324	24.139641388179847
34-35	24.209029883638202	25.830309698357333	26.19610693928354	23.764553478720927
36-37	24.279637190173883	25.589524696841664	26.148225415878656	23.982612697105797
38-39	24.04346217319946	25.703510987299953	26.22456756266495	24.02845927683563
40-41	24.2030571594156	25.558268311956923	26.30376444905623	23.934910079571246
42-43	24.04652132063851	25.49152549367133	26.243558622084162	24.218394563605997
44-45	23.999697067841634	25.568041647806496	26.253116120520364	24.17914516383151
46-47	24.017912927282907	25.535011982365674	26.180073416173745	24.267001674177678
48-49	23.807124586648015	25.637102360677623	26.17181515874109	24.383957893933275
50-51	23.86641273745253	25.69381584010713	26.209858877394897	24.22991254504544
52-53	23.935917911677972	25.494168719363003	26.227341853321224	24.3425715156378
54-55	23.702260706081592	25.44860114188162	26.32063655521201	24.528501596824775
56-57	23.60654956417183	25.57570395910525	26.222931850952936	24.59481462576999
58-59	23.520226661905	25.40159020664764	26.311320118265918	24.76686301318144
60-61	23.52945728292111	25.368782207247687	26.43586572689719	24.665894782934007
62-63	23.405719558166282	25.412257746445015	26.372106040834947	24.809916654553753
64-65	23.258379976731426	25.335468314571784	26.258948796143333	25.147202912553457
66-67	23.274540577650924	25.385494984672246	26.180224471299123	25.159739966377703
68-69	23.42027830374713	25.234094790115858	26.201312847027925	25.144314059109092
70-71	23.223987534151753	25.152632459181167	26.1992049235272	25.424175083139872
72-73	23.28087494401572	25.14607511490967	26.11016055363036	25.46288938744425
74-75	23.17735336834381	25.348452919418506	26.01570846243394	25.458485249803743
76-77	23.208819303974604	25.10711268783175	26.23182046948722	25.452247538706423
78-79	23.193649629565854	25.187240457111383	26.103864436567008	25.515245476755755
80-81	23.10836251629032	25.25231943638583	26.027456052241277	25.611861995082574
82-83	23.08173288607055	25.184528911214432	26.10165420930594	25.632083993409076
84-85	23.043721371683244	25.1583997028845	26.012039367804235	25.785839557628027
86-87	22.97750519707516	25.150430762547966	26.083980597586077	25.788083442790803
88-89	22.936714557750484	25.066569983544078	25.948550905304028	26.048164553401403
90-91	22.799274242850636	25.08669380757631	26.00978673773237	26.10424521184068
92-93	22.784511634989364	24.944124909281584	26.068883161720123	26.20248029400892
94-95	22.679367331057357	24.824752617415438	26.13118064478328	26.364699406743924
96-97	22.69665494465139	24.75965054709042	26.141483245839442	26.402211262418753
98-99	22.636649818904697	24.79132855178327	25.997829393999446	26.574192235312587
100-101	22.778446869450445	24.63204456821064	26.04790329593698	26.54160526640193
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	106.0
1	85.0
2	102.5
3	150.5
4	143.0
5	163.0
6	277.5
7	573.0
8	883.5
9	930.0
10	773.5
11	675.5
12	696.5
13	752.0
14	847.5
15	934.0
16	981.5
17	1117.0
18	1328.5
19	1484.5
20	1681.0
21	1915.5
22	2231.5
23	2692.5
24	3227.5
25	4002.0
26	5170.5
27	6746.0
28	8724.0
29	11809.5
30	16265.5
31	22842.0
32	31737.5
33	41126.5
34	51328.5
35	62925.0
36	76829.5
37	91878.5
38	104936.5
39	116143.0
40	128069.5
41	140978.0
42	152650.0
43	164369.5
44	176900.0
45	189037.5
46	199331.5
47	206879.0
48	214522.0
49	221197.0
50	223246.5
51	222052.5
52	218681.5
53	214259.0
54	207678.5
55	196281.5
56	179948.0
57	160875.5
58	143977.5
59	127229.5
60	107991.5
61	89041.0
62	71888.5
63	55296.5
64	40608.0
65	30106.5
66	22544.5
67	16938.0
68	12522.0
69	9272.0
70	6967.0
71	5090.5
72	3761.5
73	3073.0
74	2445.5
75	1777.5
76	1147.0
77	649.5
78	371.0
79	181.0
80	91.0
81	48.5
82	25.5
83	14.0
84	10.0
85	8.5
86	5.5
87	3.5
88	2.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.19378153746556526
2	0.0
3	0.011470513662392663
4	0.013657338209539465
5	0.006828669104769733
6	0.10519451288586365
7	0.13999803185790757
8	0.12163695782997687
9	0.13985361891611484
10-11	0.11266272501857252
12-13	0.11242547518562737
14-15	0.11457103889226197
16-17	0.06466605229275144
18-19	0.07731249990974191
20-21	0.03565968141267215
22-23	0.0820471813585173
24-25	0.05640356898018263
26-27	0.06433596556865381
28-29	0.038857396552367945
30-31	0.02364246161349279
32-33	0.03032671777646981
34-35	0.019537007982528512
36-37	0.02364246161349279
38-39	0.03053302197903083
40-41	0.024271689431303898
42-43	0.012708338877758777
44-45	0.025612666747950524
46-47	0.03194620576657381
48-49	0.008324374573337122
50-51	0.014771380903368967
52-53	0.006890560365538038
54-55	0.011583980973801223
56-57	0.004786257499415643
58-59	0.003465910603025121
60-61	0.02182698463095582
62-63	0.00939715642665442
64-65	0.00680803868451363
66-67	0.03949693958030711
68-69	0.08873143752149432
70-71	0.02084703966879098
72-73	0.04334451295807012
74-75	0.08435778842720072
76-77	0.24755472786309488
78-79	0.07922081378343133
80-81	0.01598857569847898
82-83	0.01124357903957554
84-85	0.012605186776478268
86-87	0.2632647928881165
88-89	0.6209756497086675
90-91	0.26430662911104963
92-93	0.3510781767082143
94-95	0.0012997164761344203
96-97	0.004249866572756994
98-99	0.0036618995954580892
100-101	0.005332963636202344
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4847211.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.339939681801525
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.1224141472043	37.02760392023377
2	13.428103040553708	13.78796000286086
3	5.521091437370383	8.50357504116919
4	2.8493294742563906	5.8513761336760926
5	1.6427771064276835	4.217003877732085
6	1.0446458662588816	3.2179233457544565
7	0.6887070074240701	2.475072335371005
8	0.4831999313533946	1.9845964263947131
9	0.36464985764934305	1.6848991527025305
>10	1.7442222023279028	15.459481144686146
>50	0.08293323798587561	2.8959094660842823
>100	0.026474435562238455	2.188570024723289
>500	0.0010891922963510924	0.3851993403544549
>1k	3.630633294998153E-4	0.3208297882571086
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.650433620488153E-4
2	0.0	0.0	0.0	0.0	1.650433620488153E-4
3	0.0	0.0	0.0	0.0	1.650433620488153E-4
4	0.0	0.0	0.0	0.0	1.650433620488153E-4
5	0.0	2.0630420256101912E-5	0.0	0.0	1.650433620488153E-4
6	0.0	2.0630420256101912E-5	0.0	0.0	2.2693462281712102E-4
7	0.0	2.0630420256101912E-5	0.0	0.0	2.4756504307322293E-4
8	0.0	2.0630420256101912E-5	0.0	0.0	2.4756504307322293E-4
9	0.0	2.0630420256101912E-5	0.0	0.0	2.4756504307322293E-4
10-11	0.0	2.0630420256101912E-5	0.0	2.0630420256101912E-5	2.681954633293248E-4
12-13	0.0	5.1576050640254775E-5	0.0	2.0630420256101912E-5	7.942711798599237E-4
14-15	0.0	6.189126076830573E-5	0.0	4.1260840512203824E-5	9.799449621648408E-4
16-17	1.0315210128050956E-5	6.189126076830573E-5	0.0	5.1576050640254775E-5	0.001144988324213656
18-19	2.0630420256101912E-5	6.189126076830573E-5	0.0	8.252168102440763E-5	0.0012481404254941655
20-21	2.0630420256101912E-5	6.189126076830573E-5	0.0	1.4441294179271338E-4	0.0015163358888234905
22-23	2.0630420256101912E-5	6.189126076830573E-5	0.0	2.1661941268907007E-4	0.001588542359719847
24-25	2.0630420256101912E-5	6.189126076830573E-5	0.0	8.355320203721273E-4	0.001588542359719847
26-27	2.0630420256101912E-5	6.189126076830573E-5	0.0	0.0017020096711284076	0.0016710640407442546
28-29	2.0630420256101912E-5	7.220647089635669E-5	0.0	0.006983397256690496	0.0017432705116406115
30-31	2.0630420256101912E-5	8.252168102440765E-5	0.0	0.030254511305573453	0.0017845313521528154
32-33	2.0630420256101912E-5	8.252168102440765E-5	0.0	0.0820265509382612	0.001867053033177223
34-35	2.0630420256101912E-5	9.28368911524586E-5	0.0	0.1509115241733855	0.002093987655994344
36-37	2.0630420256101912E-5	1.0315210128050955E-4	0.0	0.26483270482758026	0.002104302866122395
38-39	2.0630420256101912E-5	1.0315210128050955E-4	0.0	0.4522807032745222	0.0021455637066345986
40-41	2.0630420256101912E-5	1.0315210128050955E-4	0.0	0.6701379411789583	0.0021455637066345986
42-43	2.0630420256101912E-5	1.0315210128050955E-4	0.0	0.9013121153587084	0.0021455637066345986
44-45	2.0630420256101912E-5	1.0315210128050955E-4	0.0	1.1815144832770845	0.002166194126890701
46-47	2.0630420256101912E-5	1.1346731140856051E-4	0.0	1.50813529677169	0.002217770177530955
48-49	2.0630420256101912E-5	1.2378252153661147E-4	0.0	1.857552724649288	0.0023002918585553634
50-51	2.0630420256101912E-5	1.2378252153661147E-4	0.0	2.2298080277503907	0.0024550200104761274
52-53	2.0630420256101912E-5	1.2378252153661147E-4	0.0	2.613399746782222	0.0027438458940615544
54-55	2.0630420256101912E-5	1.6504336204881527E-4	0.0	3.040160620199946	0.00280573715482986
56-57	2.0630420256101912E-5	1.8567378230491719E-4	0.0	3.5297514385076285	0.002888258835854267
58-59	2.0630420256101912E-5	2.063042025610191E-4	0.0	4.05999449992996	0.002950150096622573
60-61	2.0630420256101912E-5	2.063042025610191E-4	0.0	4.621492648040285	0.0029707805168786754
62-63	2.0630420256101912E-5	2.063042025610191E-4	0.0	5.191954713751888	0.003238975980208
64-65	2.0630420256101912E-5	2.681954633293248E-4	0.0	5.802955555266729	0.0032699216105921526
66-67	2.0630420256101912E-5	2.681954633293248E-4	0.0	6.489422474078392	0.003321497661232408
68-69	2.0630420256101912E-5	2.681954633293248E-4	0.0	7.237945697020411	0.003352443291616561
70-71	2.0630420256101912E-5	2.785106734573758E-4	0.0	8.02144779750665	0.003445280182769019
72-73	4.1260840512203824E-5	2.8882588358542676E-4	0.0	8.830253108436995	0.0036618995954580892
74-75	4.1260840512203824E-5	2.8882588358542676E-4	0.0	9.666548454358598	0.0038475733777630066
76-77	4.1260840512203824E-5	2.8882588358542676E-4	0.0	10.57916604001765	0.003919779848659363
78-79	4.1260840512203824E-5	2.8882588358542676E-4	0.0	11.558450828734298	0.003981671109427669
80-81	4.1260840512203824E-5	2.8882588358542676E-4	0.0	12.56484398966746	0.004053877580324026
82-83	4.1260840512203824E-5	2.8882588358542676E-4	0.0	13.590825734633793	0.0042189209423728405
84-85	4.1260840512203824E-5	2.8882588358542676E-4	0.0	14.633404652696157	0.004291127413269197
86-87	4.1260840512203824E-5	2.8882588358542676E-4	0.0	15.709807144768405	0.00442522514493386
88-89	4.1260840512203824E-5	2.8882588358542676E-4	0.0	16.84770891962409	0.004559322876598522
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4355	0.0	49.35774	1
GTATCAA	8155	0.0	42.453384	1
TCAACGC	10580	0.0	31.988316	4
ATCAACG	10820	0.0	31.498125	3
TATCAAC	11085	0.0	31.000448	2
CAACGCA	10890	0.0	30.942804	5
AACGCAG	11805	0.0	28.705551	6
GTACATG	16470	0.0	24.398779	1
TACATGG	16800	0.0	23.336506	2
ACGCAGA	14285	0.0	23.25616	7
CGCAGAG	14810	0.0	22.17287	8
ACATGGG	17170	0.0	21.646065	3
GCAGAGT	16410	0.0	18.883389	9
CATGGGG	11670	0.0	18.09992	4
AGAGTAC	14100	0.0	17.987665	10-11
GTGGTAT	2715	0.0	17.340847	1
GAGTACA	13015	0.0	16.881178	1
GTATAGC	1515	0.0	16.322838	1
CAGAGTA	15260	0.0	16.06061	10-11
AGTACAT	12790	0.0	15.8461	2
>>END_MODULE