FastQCFastQC Report
Mon 15 Aug 2016
SRR2920885_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920885_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3213217
Sequences flagged as poor quality0
Sequence length98
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46700.14533721189698673No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40000.050.279991
GTATCAA79650.047.0918271
ATCAACG103650.036.1018873
TCAACGC104850.035.601234
CAACGCA107150.034.7942475
AACGCAG111350.033.6001636
TATCAAC112800.033.011312
GTACATG164800.029.4345321
TACATGG165550.028.3652482
ACGCAGA131500.028.0658237
CGCAGAG132850.027.8474758
ACATGGG174050.026.6634033
GCAGAGT144700.024.3947329
GAGTACA116750.022.8636111
CATGGGA98050.022.6363724
CATGGGG71950.022.3072644
AGTACAT120600.021.179892
GTCGTAC1606.3672436E-520.1350081
GAGTACT54200.019.45946312-13
AGAGTAC129750.019.132510-11
Produced by FastQC (version 0.11.5)