##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920885_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3213217
Sequences flagged as poor quality	0
Sequence length	98
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.512688685513613	31.0	28.0	33.0	22.0	34.0
2	28.509621976978213	31.0	28.0	33.0	16.0	34.0
3	28.583060527813714	31.0	28.0	34.0	18.0	34.0
4	32.065685573056534	35.0	32.0	37.0	22.0	37.0
5	32.042545834906264	35.0	32.0	37.0	22.0	37.0
6	32.17549421654373	35.0	32.0	37.0	23.0	37.0
7	32.018040487150415	35.0	32.0	37.0	22.0	37.0
8	31.9574457622999	35.0	32.0	37.0	21.0	37.0
9	33.20676505819557	37.0	33.0	39.0	19.0	39.0
10-11	33.0049483119254	37.0	32.0	39.0	18.5	39.0
12-13	32.83648552214183	37.0	32.0	39.0	17.0	39.0
14-15	33.575879251230155	38.0	32.0	40.0	17.0	41.0
16-17	33.480289846592996	38.0	32.0	40.0	17.0	41.0
18-19	33.38962479035808	38.0	32.0	40.0	17.5	41.0
20-21	33.30690395326553	38.0	32.0	40.0	16.5	41.0
22-23	33.08346183902301	38.0	32.0	40.0	14.5	41.0
24-25	32.88999217917744	38.0	32.0	40.0	11.0	41.0
26-27	32.92863740606377	38.0	32.0	40.0	10.0	41.0
28-29	32.77399845699808	38.0	31.5	40.0	10.0	41.0
30-31	32.515539411125985	37.5	31.0	40.0	9.0	41.0
32-33	32.25949539044515	37.0	30.5	40.0	8.5	41.0
34-35	31.92135156137914	37.0	30.0	40.0	5.0	41.0
36-37	31.604901878709093	37.0	30.0	40.0	2.0	41.0
38-39	31.401160737043284	37.0	30.0	40.0	2.0	41.0
40-41	31.1993050889498	36.5	30.0	40.0	2.0	41.0
42-43	30.94086813931334	36.0	29.5	40.0	2.0	41.0
44-45	30.64768859370531	36.0	29.0	39.0	2.0	40.5
46-47	30.368843125129736	36.0	28.0	39.0	2.0	40.0
48-49	30.05362522979307	35.0	27.5	39.0	2.0	40.0
50-51	28.913098461759663	34.0	25.5	38.0	2.0	39.5
52-53	29.36053789706702	34.5	27.0	38.5	2.0	39.5
54-55	30.003573677096817	35.0	28.0	39.0	2.0	40.0
56-57	29.959416528668932	35.0	28.0	39.0	2.0	41.0
58-59	29.727586247676392	35.0	28.0	39.0	2.0	40.5
60-61	29.420332800430224	35.0	27.0	39.0	2.0	40.0
62-63	29.042702375843277	35.0	26.0	38.5	2.0	40.0
64-65	28.638925102163967	34.0	26.0	38.0	2.0	40.0
66-67	28.268614911473456	34.0	26.0	38.0	2.0	40.0
68-69	27.836874384767665	34.0	25.0	37.0	2.0	40.0
70-71	27.515233954009332	34.0	24.5	36.5	2.0	39.0
72-73	27.065336701505068	33.0	23.5	36.0	2.0	39.0
74-75	26.62793378100514	33.0	22.0	36.0	2.0	39.0
76-77	26.22010682751896	33.0	21.0	35.0	2.0	37.5
78-79	25.75083771186322	32.0	20.0	35.0	2.0	37.0
80-81	25.293123215767874	32.0	18.0	35.0	2.0	36.5
82-83	24.82055833764106	32.0	13.5	35.0	2.0	36.0
84-85	24.41681296345687	31.5	7.5	35.0	2.0	35.5
86-87	23.958886530228117	31.0	3.5	34.0	2.0	35.0
88-89	23.42315894009026	31.0	2.0	34.0	2.0	35.0
90-91	22.915154656532692	31.0	2.0	34.0	2.0	35.0
92-93	22.380122630995665	30.0	2.0	34.0	2.0	35.0
94-95	21.736479360093014	30.0	2.0	34.0	2.0	35.0
96-97	21.09794358737676	29.0	2.0	34.0	2.0	35.0
98	20.69975168188143	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	169235.0
3	27980.0
4	20635.0
5	16583.0
6	15550.0
7	15972.0
8	18457.0
9	21017.0
10	22950.0
11	24689.0
12	25985.0
13	25961.0
14	25085.0
15	26502.0
16	29628.0
17	32034.0
18	32843.0
19	30606.0
20	30702.0
21	32836.0
22	35637.0
23	39704.0
24	45380.0
25	51868.0
26	59555.0
27	68973.0
28	80068.0
29	92198.0
30	106953.0
31	125469.0
32	150268.0
33	180974.0
34	223652.0
35	282738.0
36	357363.0
37	393903.0
38	245976.0
39	27286.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.60711614441992	17.851284803866125	12.259358982129658	24.282240069584297
2	16.19054585978636	20.096094731288066	39.03014983867478	24.68320957025079
3	20.050027407374436	24.398664151185365	28.484495145579693	27.066813295860502
4	13.030238702351665	15.223842594898255	36.39625686026475	35.34966184248533
5	14.247533489907502	37.564042103821436	33.30105352937789	14.887370876893169
6	33.274791417186485	34.571159712038515	17.52360223911934	14.630446631655655
7	29.884415985418002	31.888693969735378	20.251418731993496	17.975471312853127
8	27.273194140375274	33.675474695462235	20.225266752611816	18.826064411550682
9	28.051451240299052	15.427124903173361	19.368906612905384	37.1525172436222
10-11	25.978933262210425	25.627774283529558	26.994628747451543	21.398663706808474
12-13	26.62259531217564	22.954177140888383	27.28948565022547	23.133741896710504
14-15	23.496650664988536	24.315439246298084	25.215364412037534	26.972545676675846
16-17	23.4564777367312	27.70883685160036	25.768152762365208	23.06653264930323
18-19	23.387348973642645	26.30014942108087	27.796181488940054	22.51632011633643
20-21	24.276011352531935	25.710930899832203	27.41890297789838	22.594154769737482
22-23	23.943703873076714	25.567969685646524	27.17385598844147	23.314470452835295
24-25	23.542001045098036	25.738715525934065	27.34841874425056	23.37086468471734
26-27	23.1278878268095	26.039586507425348	27.290438295651175	23.542087370113972
28-29	23.29706447200743	26.071620686407076	27.40632101095072	23.224993830634773
30-31	23.32524163769028	26.1740457720527	27.05171347052473	23.44899911973229
32-33	23.043992475215042	26.128316976041244	26.97128339232272	23.856407156420993
34-35	23.566394062817444	26.022982662295263	26.992449553090616	23.41817372179668
36-37	23.625682970359907	25.77935658052598	26.932987957197135	23.661972491916977
38-39	23.467923665141385	25.8802056355112	27.23397848366246	23.417892215684954
40-41	23.530752801896238	25.750019428023357	27.240621815582795	23.478605954497613
42-43	23.559010950163366	25.655117207975586	26.880724080451024	23.905147761410028
44-45	23.326731454245632	25.903714228841046	26.977118520395777	23.792435796517545
46-47	23.289634553623255	25.848595095039183	26.822118806203815	24.039651545133747
48-49	23.48734105231057	25.869712888042333	26.657670983594674	23.985275076052424
50-51	23.182727403955212	26.108688601728836	26.75240850132072	23.95617549299524
52-53	23.433808049690466	25.813319360911773	26.850902965658978	23.90196962373878
54-55	23.008644133052027	25.78263254407689	27.165922054145764	24.04280126872532
56-57	23.00252831362778	25.945179709669773	26.982758741934642	24.069533234767803
58-59	23.000143124821093	25.4863275170906	27.16516916853854	24.348360189549766
60-61	22.95131820324866	25.598538675666227	27.27240762685285	24.177735494232262
62-63	22.88135765351106	25.559935359653718	27.268407897896623	24.2902990889386
64-65	22.685811307211488	25.373604668393618	27.293539692037584	24.64704433235731
66-67	22.714821027622474	25.711950321349935	27.068504047928805	24.504724603098786
68-69	23.01336033913301	25.288826058700735	27.10981117139427	24.588002430771986
70-71	22.579984605368878	25.266422710079862	27.35838063975189	24.795212044799374
72-73	22.798557013025082	25.32344063592878	27.164703010585328	24.71329934046081
74-75	22.608023137124658	25.34660951089846	27.217756700117874	24.827610651859004
76-77	22.711089826956595	25.08168280076017	27.40515153223272	24.802075840050517
78-79	22.68931286315036	25.34965176913521	27.22908663019575	24.73194873751868
80-81	22.634650378459686	25.32725190854	27.24780129820075	24.790296414799563
82-83	22.86095989455964	25.10072427783035	27.088459340185704	24.949856487424302
84-85	22.66574510574318	24.925573440555883	27.31785793928067	25.09082351442026
86-87	22.77502529076771	24.818007182749785	27.430290930876687	24.976676595605817
88-89	22.714311823136413	24.664959847042304	27.38230208208042	25.238426247740858
90-91	22.671912208237444	24.715640047276715	27.498191515678528	25.114256228807314
92-93	22.973302378936104	24.504495669700113	27.47849763008975	25.043704321274035
94-95	22.8291843429197	24.301176022294527	27.60334605320796	25.26629358157781
96-97	23.080363563845196	24.066732184076958	27.608545290172216	25.24435896190563
98	23.063466939175278	23.995020388174858	27.466007215814827	25.475505456835034
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	178.0
1	143.0
2	219.0
3	363.0
4	357.0
5	388.0
6	608.0
7	1110.0
8	1690.0
9	1941.5
10	1813.0
11	1715.0
12	1780.0
13	1955.0
14	2259.5
15	2471.5
16	2625.0
17	2752.5
18	2835.5
19	2920.0
20	3047.0
21	3256.0
22	3588.5
23	4144.5
24	4851.0
25	5823.0
26	7265.0
27	9426.5
28	12448.0
29	16597.5
30	22273.5
31	29491.0
32	35969.5
33	41281.5
34	47835.5
35	56886.0
36	66661.5
37	75309.0
38	81772.5
39	88373.0
40	95579.5
41	101592.5
42	109619.5
43	117260.0
44	122026.5
45	125993.0
46	130336.0
47	138513.5
48	145519.0
49	145565.5
50	144407.0
51	140931.5
52	137165.0
53	135417.5
54	128606.5
55	119424.5
56	108846.0
57	97225.5
58	88571.0
59	78487.5
60	67155.0
61	55305.5
62	44090.0
63	33997.0
64	24930.5
65	18515.5
66	13510.5
67	10154.0
68	7513.5
69	5582.0
70	4230.0
71	3471.5
72	3055.0
73	2597.0
74	2174.5
75	1565.0
76	975.0
77	549.5
78	315.5
79	195.0
80	111.5
81	53.0
82	36.0
83	29.0
84	19.0
85	11.5
86	9.5
87	10.5
88	8.5
89	4.5
90	2.0
91	3.5
92	6.0
93	5.0
94	2.5
95	2.0
96	2.5
97	2.5
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3888626258357279
2	0.023714551491542588
3	0.01798820309988401
4	0.015965308287613316
5	0.02122483479951712
6	0.021909506889824123
7	0.015218393280005679
8	0.009803259474850282
9	0.0
10-11	0.0
12-13	0.0052906479705541205
14-15	0.0036256499327620884
16-17	0.0062398524593888305
18-19	0.03589860255314222
20-21	0.07716565672346437
22-23	0.09711451171831843
24-25	0.06333216835339786
26-27	0.07235739136199018
28-29	0.05553624296149311
30-31	0.052719750953639295
32-33	0.019715444054976678
34-35	0.3389126847019669
36-37	0.5197906023776172
38-39	0.47393313305637313
40-41	0.4828961131476648
42-43	0.4327127610740264
44-45	0.40825129457487624
46-47	0.4073643330033422
48-49	0.3981368205135227
50-51	0.3945889742273864
52-53	0.4523504014823774
54-55	0.45354857764041456
56-57	0.4249168356821217
58-59	0.41120783314665643
60-61	0.4249168356821217
62-63	0.4060883531986791
64-65	0.451727972309371
66-67	0.47377752576312154
68-69	0.41735432123009436
70-71	0.37660077112750245
72-73	0.3629851329679881
74-75	0.34515253716135574
76-77	0.3436898286047908
78-79	0.3433008103716618
80-81	0.3596084547044286
82-83	0.3550024788241815
84-85	0.3548779929895802
86-87	0.34113786899546467
88-89	0.383789828075726
90-91	0.36228490014835596
92-93	0.3517502863952232
94-95	0.3589704648020971
96-97	0.3586281287569436
98	0.3535708917262668
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3213217.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.239740260041685
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.37065704663497	33.19269467498363
2	11.129711902550023	10.070105512809148
3	5.090690585001754	6.90904559429157
4	2.8252454561462548	5.112534824276783
5	1.8259872123196144	4.130359360174847
6	1.2124442374390199	3.291039742891534
7	0.8750975284083088	2.7712429421177434
8	0.6197165922830569	2.2428654135778916
9	0.4741859985917281	1.930684627011444
>10	2.370868113719509	18.612273071036583
>50	0.12825840323626053	3.9863580241372945
>100	0.0725446533232127	5.943564433309597
>500	0.003687732248049459	1.1185931499432797
>1k	9.045380983182626E-4	0.6886386294387755
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4670	0.14533721189698673	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.1121458650318356E-5	0.0
5	0.0	0.0	0.0	3.1121458650318356E-5	0.0
6	0.0	0.0	0.0	3.1121458650318356E-5	0.0
7	0.0	0.0	0.0	3.1121458650318356E-5	0.0
8	0.0	0.0	0.0	3.1121458650318356E-5	0.0
9	0.0	0.0	0.0	3.1121458650318356E-5	0.0
10-11	0.0	0.0	0.0	4.6682187975477534E-5	0.0
12-13	0.0	0.0	0.0	6.224291730063671E-5	0.0
14-15	0.0	0.0	0.0	6.224291730063671E-5	0.0
16-17	0.0	0.0	0.0	7.780364662579589E-5	0.0
18-19	0.0	0.0	0.0	1.0892510527611425E-4	0.0
20-21	0.0	1.5560729325159178E-5	0.0	1.7116802257675096E-4	0.0
22-23	0.0	3.1121458650318356E-5	0.0	4.35700421104457E-4	0.0
24-25	0.0	3.1121458650318356E-5	0.0	0.0011826154287120976	0.0
26-27	0.0	3.1121458650318356E-5	0.0	0.0027075669025776973	0.0
28-29	0.0	3.1121458650318356E-5	0.0	0.010954753444912061	0.0
30-31	0.0	3.1121458650318356E-5	0.0	0.0420450906365801	0.0
32-33	0.0	3.1121458650318356E-5	0.0	0.10428800793721682	0.0
34-35	0.0	3.1121458650318356E-5	0.0	0.19365327645160596	0.0
36-37	0.0	3.1121458650318356E-5	0.0	0.3342600266337443	0.0
38-39	0.0	3.1121458650318356E-5	0.0	0.5677020879697823	0.0
40-41	0.0	3.1121458650318356E-5	0.0	0.841788774303136	0.0
42-43	0.0	3.1121458650318356E-5	0.0	1.11394593020017	0.0
44-45	0.0	3.1121458650318356E-5	0.0	1.4376713430807817	0.0
46-47	0.0	3.1121458650318356E-5	0.0	1.8225815436679191	0.0
48-49	0.0	3.1121458650318356E-5	0.0	2.2500970211473423	0.0
50-51	0.0	3.1121458650318356E-5	0.0	2.7269711320461703	0.0
52-53	0.0	3.1121458650318356E-5	0.0	3.1962982892222964	0.0
54-55	0.0	3.1121458650318356E-5	0.0	3.7018197028087427	0.0
56-57	0.0	3.1121458650318356E-5	0.0	4.2897507389012315	0.0
58-59	0.0	3.1121458650318356E-5	0.0	4.936999275181227	0.0
60-61	0.0	3.1121458650318356E-5	0.0	5.6383057851368275	0.0
62-63	0.0	3.1121458650318356E-5	0.0	6.327008104339047	0.0
64-65	0.0	3.1121458650318356E-5	0.0	7.028641389610474	0.0
66-67	0.0	3.1121458650318356E-5	0.0	7.81655891898991	0.0
68-69	0.0	3.1121458650318356E-5	0.0	8.695693443673427	0.0
70-71	0.0	3.1121458650318356E-5	0.0	9.646811902215132	0.0
72-73	0.0	3.1121458650318356E-5	0.0	10.573826791032165	0.0
74-75	0.0	3.1121458650318356E-5	0.0	11.502973499766744	0.0
76-77	0.0	3.1121458650318356E-5	0.0	12.492013455673863	0.0
78-79	0.0	3.1121458650318356E-5	0.0	13.577498811938316	0.0
80-81	0.0	3.1121458650318356E-5	0.0	14.727436709067579	0.0
82-83	0.0	3.1121458650318356E-5	0.0	15.889465292882491	0.0
84-85	0.0	3.1121458650318356E-5	0.0	17.021477229829173	0.0
86	0.0	3.1121458650318356E-5	0.0	17.86521109529795	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4000	0.0	50.27999	1
GTATCAA	7965	0.0	47.091827	1
ATCAACG	10365	0.0	36.101887	3
TCAACGC	10485	0.0	35.60123	4
CAACGCA	10715	0.0	34.794247	5
AACGCAG	11135	0.0	33.600163	6
TATCAAC	11280	0.0	33.01131	2
GTACATG	16480	0.0	29.434532	1
TACATGG	16555	0.0	28.365248	2
ACGCAGA	13150	0.0	28.065823	7
CGCAGAG	13285	0.0	27.847475	8
ACATGGG	17405	0.0	26.663403	3
GCAGAGT	14470	0.0	24.394732	9
GAGTACA	11675	0.0	22.863611	1
CATGGGA	9805	0.0	22.636372	4
CATGGGG	7195	0.0	22.307264	4
AGTACAT	12060	0.0	21.17989	2
GTCGTAC	160	6.3672436E-5	20.135008	1
GAGTACT	5420	0.0	19.459463	12-13
AGAGTAC	12975	0.0	19.1325	10-11
>>END_MODULE