FastQCFastQC Report
Mon 15 Aug 2016
SRR2920885_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920885_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3213217
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42190.13130143404569314No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43250.049.148681
GTATCAA82900.047.66911
ATCAACG110250.035.173933
TCAACGC111350.034.8264544
CAACGCA112450.034.313195
TATCAAC117100.033.316952
AACGCAG116950.033.2786
GTACATG174300.029.6566851
TACATGG174300.029.1909182
ACGCAGA140400.027.1785167
ACATGGG182150.026.9444163
CGCAGAG142150.026.7099558
CATGGGA99950.024.4569234
GCAGAGT152650.023.5047179
GAGTACA122800.023.2350351
CATGGGG74850.022.0680944
AGTACAT130200.020.7785022
GTGGTAT25550.020.1012611
ATGGGAT39550.019.9201055
ATGGGAG42600.019.8308095
Produced by FastQC (version 0.11.5)