##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920885_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3213217
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.18869220472816	31.0	30.0	34.0	26.0	34.0
2	30.179777774112363	31.0	30.0	34.0	26.0	34.0
3	30.321706252643377	31.0	30.0	34.0	26.0	34.0
4	33.9673402076486	35.0	35.0	37.0	30.0	37.0
5	33.49605115371916	35.0	35.0	37.0	28.0	37.0
6	33.445495900214645	35.0	35.0	37.0	28.0	37.0
7	33.25677381888618	35.0	33.0	37.0	27.0	37.0
8	33.18904014263587	35.0	33.0	37.0	27.0	37.0
9	34.497499235190155	38.0	34.0	39.0	27.0	39.0
10-11	34.308858692083355	37.5	34.0	39.0	27.0	39.0
12-13	34.12490535186388	37.0	34.0	39.0	26.0	39.0
14-15	35.01319238632187	38.0	34.0	40.0	25.5	41.0
16-17	34.90194313051375	38.0	34.0	40.0	25.0	41.0
18-19	34.83057898050458	38.5	34.0	40.0	25.0	41.0
20-21	34.72920829810125	38.0	34.0	40.0	25.0	41.0
22-23	34.58188429228403	38.0	33.5	40.0	24.0	41.0
24-25	34.40176169240982	38.0	33.0	40.0	23.5	41.0
26-27	34.23084575364814	38.0	33.0	40.0	23.0	41.0
28-29	34.166749864699455	38.0	33.0	40.0	22.5	41.0
30-31	34.138604862354455	38.0	33.0	40.0	22.0	41.0
32-33	33.97611210198377	38.0	33.0	40.0	21.5	41.0
34-35	33.79772250053451	38.0	33.0	40.0	19.5	41.0
36-37	33.60194222799145	38.0	33.0	40.0	18.5	41.0
38-39	33.412439153658156	38.0	32.0	40.0	17.0	41.0
40-41	33.17429386188359	38.0	32.0	40.0	15.5	41.0
42-43	33.16578945648551	38.0	32.0	40.0	15.0	41.0
44-45	33.00397436587694	38.0	32.0	40.0	14.0	41.0
46-47	32.73418819830718	37.5	31.5	40.0	9.0	41.0
48-49	32.45998807425704	37.0	31.0	40.0	8.5	41.0
50-51	32.197278148347905	37.0	31.0	40.0	7.5	41.0
52-53	31.946852640204504	37.0	30.5	40.0	6.0	41.0
54-55	31.66874303851872	36.0	30.0	40.0	2.0	41.0
56-57	31.35707221143172	36.0	30.0	39.0	2.0	41.0
58-59	31.0353784073718	35.5	29.0	39.0	2.0	41.0
60-61	30.723675525182394	35.0	29.0	39.0	2.0	40.0
62-63	30.417741160961118	35.0	28.5	39.0	2.0	40.0
64-65	30.02434133766876	35.0	28.0	38.0	2.0	40.0
66-67	29.60078170879838	34.0	28.0	38.0	2.0	40.0
68-69	29.174136387302816	34.0	27.5	37.0	2.0	40.0
70-71	28.75908038579405	34.0	26.5	37.0	2.0	39.0
72-73	28.32898711789462	34.0	26.0	36.0	2.0	39.0
74-75	27.886101063202393	33.5	26.0	36.0	2.0	38.5
76-77	26.425637764271755	31.5	24.0	34.5	2.0	36.5
78-79	27.256816455284536	33.0	26.0	35.0	2.0	37.0
80-81	27.27451569564085	33.0	26.0	35.0	2.0	37.0
82-83	26.93149404475328	33.0	25.0	35.0	2.0	36.0
84-85	26.611562337682145	33.0	25.0	35.0	2.0	36.0
86-87	26.076469936515338	32.5	23.5	35.0	2.0	35.5
88-89	25.708163500940024	32.0	21.5	35.0	2.0	35.0
90-91	25.346557982234003	32.0	20.0	35.0	2.0	35.0
92-93	25.04425175766218	32.0	19.0	34.0	2.0	35.0
94-95	24.669120386204852	32.0	16.5	34.0	2.0	35.0
96-97	24.15297270616955	32.0	4.5	34.0	2.0	35.0
98-99	23.57070297462014	31.0	2.0	34.0	2.0	35.0
100-101	21.42179130758987	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	81442.0
3	35427.0
4	18830.0
5	13125.0
6	11174.0
7	11395.0
8	12837.0
9	14701.0
10	16778.0
11	18570.0
12	20895.0
13	23073.0
14	25421.0
15	27496.0
16	30030.0
17	31327.0
18	29088.0
19	27022.0
20	26791.0
21	27495.0
22	29979.0
23	33752.0
24	39233.0
25	46038.0
26	54185.0
27	64159.0
28	75735.0
29	89765.0
30	105251.0
31	124501.0
32	150746.0
33	183030.0
34	230996.0
35	299903.0
36	397868.0
37	473491.0
38	288640.0
39	23028.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.69362570995044	19.1886031243744	12.900907227321968	24.216863938353196
2	16.184839056932663	21.113980163804687	38.008326235047306	24.692854544215344
3	19.698457583147487	25.139978492381292	28.937053642989092	26.22451028148213
4	13.380772683300943	16.12569580964167	35.96786483084558	34.525666676211806
5	14.120661289750307	37.8394730895856	33.391596747457356	14.648268873206735
6	33.24562146857452	35.111155005121894	17.236627627305786	14.406595898997804
7	29.703613368143806	32.0890366024198	20.298833171840915	17.908516857595476
8	27.217814621518126	34.1406923594421	20.033752655232767	18.607740363807007
9	27.770452064225402	15.438767302128598	19.31692491235375	37.473855721292246
10-11	25.899880855051943	25.822252811621365	27.03093219705278	21.246934136273904
12-13	26.581067677041958	23.009717555738035	27.382251537271923	23.026963229948088
14-15	23.415074304686602	24.444631043954672	25.228790386504013	26.911504264854706
16-17	23.47091722699583	27.883106476710644	25.726637224666383	22.91933907162715
18-19	23.356277768866477	26.436848555824277	27.76195200044851	22.444921674860737
20-21	24.262791634573293	25.78115319450732	27.323285124262835	22.63277004665655
22-23	23.950140297964	25.721116895450646	27.113475098882926	23.215267707702424
24-25	23.507761361394653	25.940853999650297	27.278666481696263	23.272718157258787
26-27	23.165622643395604	26.246836904563004	27.186979483848845	23.400560968192547
28-29	23.30496212112367	26.18422373794807	27.34408870810187	23.16672543282639
30-31	23.374748941288683	26.268243392159622	27.013061278870975	23.343946387680724
32-33	23.075828678628287	26.24143800966452	26.89837872436073	23.784354587346463
34-35	23.564509208130506	26.096922135668084	26.969463841782677	23.369104814418744
36-37	23.6512076417961	25.894114393162702	26.883580806294034	23.571097158747165
38-39	23.459428412213775	25.976245197011078	27.209468299300244	23.354858091474902
40-41	23.533290135944146	25.781603733424895	27.246978919559933	23.438127211071023
42-43	23.530318069638604	25.743583374808228	26.89857746560852	23.82752108994465
44-45	23.278591307271334	25.981434094772037	26.951568015838923	23.78840658211771
46-47	23.248640191673704	25.930306894920513	26.807389686361883	24.0136632270439
48-49	23.39203637809502	25.954345304002818	26.69376556701881	23.95985275088335
50-51	23.138562943512415	26.12822337306931	26.79186953303539	23.941344150382886
52-53	23.465178298125103	25.856289916895513	26.771726205713755	23.90680557926563
54-55	22.977474809379846	25.8587905950038	26.997531439058044	24.166203156558307
56-57	22.962121715514723	26.02134146104244	26.765729605422152	24.250807218020682
58-59	22.876787492168756	25.6273320612457	27.004775152648957	24.491105293936585
60-61	22.84805679889256	25.847100832789355	26.993497500511292	24.311344867806792
62-63	22.7732750678519	25.806908443514853	26.8988726625333	24.52094382609995
64-65	22.456820868716488	25.65279320780403	26.928897660942024	24.96148826253746
66-67	22.513093813897772	26.041655802180475	26.621838823693324	24.823411560228433
68-69	22.848359937631795	25.674335601197342	26.591464726156318	24.885839735014553
70-71	22.418489447592982	25.65933185961709	26.71872040382087	25.20345828896906
72-73	22.579183801569727	25.85250553589176	26.38535846922941	25.182952193309106
74-75	22.35675214000013	25.910097723116486	26.283478336168393	25.44967180071499
76-77	22.384503414092947	25.615860373106514	26.67232523199624	25.327310980804295
78-79	22.34146297402572	25.944625336148142	26.329284086215885	25.384627603610248
80-81	22.170062317450924	26.077303147372888	26.260079837181827	25.492554697994358
82-83	22.292323149574145	25.93391242855204	25.981565150702124	25.79219927117169
84-85	22.06251627290326	25.75807023171058	26.113167741514786	26.066245753871375
86-87	22.06062905771779	25.75704409120774	26.186377567251768	25.995949283822707
88-89	21.95170684936698	25.73926308653936	26.058705728029352	26.250324336064306
90-91	21.756880797643756	25.85369385180032	26.03374817078698	26.35567717976895
92-93	21.88502785488134	25.756586986480524	26.012547384530098	26.345837774108038
94-95	21.657590496132176	25.542874090930596	26.071541054707243	26.72799435822999
96-97	21.75140904455738	25.42193529793751	26.11934991378214	26.707305743722966
98-99	21.623577292830294	25.443274013311406	25.952920668567526	26.980228025290774
100-101	21.592824753703376	25.476973333563645	26.11602415340083	26.814177759332146
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	180.0
1	142.5
2	181.0
3	285.5
4	264.0
5	265.5
6	489.5
7	957.5
8	1431.0
9	1513.0
10	1239.0
11	1055.0
12	1061.0
13	1115.0
14	1175.0
15	1256.5
16	1409.5
17	1578.0
18	1695.0
19	1877.5
20	2069.5
21	2339.5
22	2772.5
23	3328.0
24	3972.5
25	4801.5
26	6066.0
27	7856.5
28	10071.5
29	13455.5
30	18213.5
31	23877.5
32	31196.5
33	37424.0
34	42052.5
35	48610.0
36	57982.5
37	67270.0
38	74749.5
39	81207.5
40	87226.5
41	94604.5
42	102120.5
43	109878.5
44	116586.5
45	120995.0
46	124958.5
47	129994.5
48	138759.0
49	145127.5
50	144315.5
51	141089.5
52	136663.0
53	134015.0
54	129486.5
55	119907.5
56	109179.5
57	97874.0
58	88772.0
59	79504.5
60	67783.5
61	55864.5
62	45127.5
63	34432.5
64	25270.5
65	18860.0
66	13818.0
67	10098.0
68	7394.5
69	5337.5
70	4113.5
71	3552.0
72	3123.5
73	2800.5
74	2226.0
75	1667.0
76	1081.0
77	566.0
78	294.5
79	162.5
80	91.5
81	38.5
82	21.0
83	10.5
84	7.0
85	6.5
86	2.5
87	3.0
88	2.5
89	2.0
90	2.5
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1961585538729566
2	0.0
3	0.012510826377427979
4	0.012853162422581482
5	0.006940085279020994
6	0.10783585422335311
7	0.1432831956260657
8	0.1241123770974696
9	0.14253628061845808
10-11	0.11455808929182187
12-13	0.11566290107390817
14-15	0.11605191930703716
16-17	0.06716010776738701
18-19	0.07973317706211562
20-21	0.03843500143314317
22-23	0.08491489992739364
24-25	0.0588973604957275
26-27	0.06726903287266313
28-29	0.041531586568849845
30-31	0.02555071755191137
32-33	0.03283313887608586
34-35	0.020182265934731453
36-37	0.02422805555927284
38-39	0.03188393438725116
40-41	0.025877492867739715
42-43	0.013382227219636892
44-45	0.027293519236329198
46-47	0.033969072116822485
48-49	0.009165269572518757
50-51	0.01590306537031268
52-53	0.0066444314218429695
54-55	0.0128842838812318
56-57	0.005461815993130871
58-59	0.0039213037899401134
60-61	0.023652308574241953
62-63	0.010487931565157286
64-65	0.007329103512149973
66-67	0.0404423355160887
68-69	0.09110807019880698
70-71	0.021893946160498962
72-73	0.0450949935843113
74-75	0.08776251339389776
76-77	0.25041881702978663
78-79	0.08026224185917105
80-81	0.01722572736295121
82-83	0.01210624741497384
84-85	0.013615638159514281
86-87	0.2675978622047624
88-89	0.6272685598264917
90-91	0.26853150596427194
92-93	0.3533686022450398
94-95	0.0012759798046630526
96-97	0.004216957647118137
98-99	0.003376678263559542
100-101	0.00530620869987928
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3213217.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.30475322832996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.01274053406237	30.041764643609326
2	11.791781155282434	9.976967837933897
3	5.540820813610789	7.032091716065965
4	3.1251144893873954	5.288287891352486
5	2.0344593297928504	4.303364994998004
6	1.3477309005633513	3.4209253899916483
7	0.9489381410523592	2.8101215720319153
8	0.7303518805348548	2.4717884860699
9	0.5128117617025043	1.9524937528268633
>10	2.712709941537923	19.818444638833444
>50	0.1536610716218438	4.46230363364428
>100	0.08355020919759128	6.46300999851974
>500	0.004367451772018362	1.2721155875747392
>1k	9.623198817621938E-4	0.6863198565478185
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4219	0.13130143404569314	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.5560729325159178E-5	0.0
12-13	0.0	0.0	0.0	4.6682187975477534E-5	0.0
14-15	0.0	0.0	0.0	6.224291730063671E-5	0.0
16-17	0.0	0.0	0.0	7.780364662579589E-5	0.0
18-19	0.0	0.0	0.0	1.0892510527611425E-4	0.0
20-21	0.0	1.5560729325159178E-5	0.0	1.7116802257675096E-4	0.0
22-23	0.0	3.1121458650318356E-5	0.0	4.979433384050937E-4	0.0
24-25	0.0	3.1121458650318356E-5	0.0	0.0012448583460127343	0.0
26-27	0.0	3.1121458650318356E-5	0.0	0.002878734925154448	0.0
28-29	0.0	3.1121458650318356E-5	0.0	0.011514939700617791	0.0
30-31	3.1121458650318356E-5	3.1121458650318356E-5	0.0	0.04279200564418774	0.0
32-33	3.1121458650318356E-5	3.1121458650318356E-5	0.0	0.10573515576445662	0.0
34-35	3.1121458650318356E-5	3.1121458650318356E-5	0.0	0.1961585538729566	0.0
36-37	3.1121458650318356E-5	3.1121458650318356E-5	0.0	0.3366096967618434	0.0
38-39	3.1121458650318356E-5	3.1121458650318356E-5	0.0	0.5724947926019313	0.0
40-41	4.6682187975477534E-5	3.1121458650318356E-5	0.0	0.8503782968906239	0.0
42-43	6.224291730063671E-5	3.1121458650318356E-5	0.0	1.1255386735474135	0.0
44-45	6.224291730063671E-5	3.1121458650318356E-5	0.0	1.4536210906390699	0.0
46-47	6.224291730063671E-5	3.1121458650318356E-5	0.0	1.8420946982416688	0.0
48-49	6.224291730063671E-5	3.1121458650318356E-5	0.0	2.2707149875031782	0.0
50-51	6.224291730063671E-5	3.1121458650318356E-5	0.0	2.746359800785319	0.0
52-53	6.224291730063671E-5	3.1121458650318356E-5	0.0	3.2117189719835295	0.0
54-55	6.224291730063671E-5	3.1121458650318356E-5	0.0	3.713459128343962	0.0
56-57	7.780364662579589E-5	3.1121458650318356E-5	0.0	4.296255123759148	0.0
58-59	9.336437595095507E-5	3.1121458650318356E-5	0.0	4.938773198324297	0.0
60-61	9.336437595095507E-5	3.1121458650318356E-5	0.0	5.635318125106396	0.0
62-63	9.336437595095507E-5	3.1121458650318356E-5	0.0	6.321608531263217	0.0
64-65	9.336437595095507E-5	3.1121458650318356E-5	0.0	7.021187800263723	0.0
66-67	9.336437595095507E-5	3.1121458650318356E-5	0.0	7.801698422484383	0.0
68-69	9.336437595095507E-5	3.1121458650318356E-5	0.0	8.67292809667072	0.0
70-71	9.336437595095507E-5	3.1121458650318356E-5	0.0	9.618522496301992	0.0
72-73	9.336437595095507E-5	3.1121458650318356E-5	0.0	10.543234397178903	1.5560729325159178E-5
74-75	9.336437595095507E-5	3.1121458650318356E-5	0.0	11.473268067485016	3.1121458650318356E-5
76-77	9.336437595095507E-5	3.1121458650318356E-5	0.0	12.46247919141471	3.1121458650318356E-5
78-79	9.336437595095507E-5	3.1121458650318356E-5	0.0	13.54508581275401	3.1121458650318356E-5
80-81	9.336437595095507E-5	3.1121458650318356E-5	0.0	14.69239394662732	3.1121458650318356E-5
82-83	9.336437595095507E-5	3.1121458650318356E-5	0.0	15.858390516420148	3.1121458650318356E-5
84-85	9.336437595095507E-5	3.1121458650318356E-5	0.0	16.995677540608057	3.1121458650318356E-5
86-87	9.336437595095507E-5	3.1121458650318356E-5	0.0	18.13466068429241	3.1121458650318356E-5
88-89	9.336437595095507E-5	3.1121458650318356E-5	0.0	19.34670145215838	3.1121458650318356E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4325	0.0	49.14868	1
GTATCAA	8290	0.0	47.6691	1
ATCAACG	11025	0.0	35.17393	3
TCAACGC	11135	0.0	34.826454	4
CAACGCA	11245	0.0	34.31319	5
TATCAAC	11710	0.0	33.31695	2
AACGCAG	11695	0.0	33.278	6
GTACATG	17430	0.0	29.656685	1
TACATGG	17430	0.0	29.190918	2
ACGCAGA	14040	0.0	27.178516	7
ACATGGG	18215	0.0	26.944416	3
CGCAGAG	14215	0.0	26.709955	8
CATGGGA	9995	0.0	24.456923	4
GCAGAGT	15265	0.0	23.504717	9
GAGTACA	12280	0.0	23.235035	1
CATGGGG	7485	0.0	22.068094	4
AGTACAT	13020	0.0	20.778502	2
GTGGTAT	2555	0.0	20.101261	1
ATGGGAT	3955	0.0	19.920105	5
ATGGGAG	4260	0.0	19.830809	5
>>END_MODULE