##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920884_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3075822
Sequences flagged as poor quality	0
Sequence length	98
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.47431580891222	31.0	28.0	33.0	20.0	34.0
2	28.497353878085274	31.0	28.0	33.0	16.0	34.0
3	28.572579947734297	31.0	28.0	34.0	18.0	34.0
4	32.031235552642514	35.0	32.0	37.0	22.0	37.0
5	31.99677517099494	35.0	32.0	37.0	22.0	37.0
6	32.124169734139365	35.0	32.0	37.0	23.0	37.0
7	31.95493497348026	35.0	32.0	37.0	21.0	37.0
8	31.892186543954754	35.0	32.0	37.0	19.0	37.0
9	33.15454762986935	37.0	33.0	39.0	19.0	39.0
10-11	32.956317368170204	37.0	32.0	39.0	18.0	39.0
12-13	32.78878800528769	37.0	32.0	39.0	17.0	39.0
14-15	33.53723134823797	38.0	32.0	40.0	17.0	41.0
16-17	33.423848161564614	38.0	32.0	40.0	17.0	41.0
18-19	33.30911070276498	38.0	32.0	40.0	17.0	41.0
20-21	33.221902795415346	38.0	32.0	40.0	16.0	41.0
22-23	32.99522095231779	38.0	32.0	40.0	11.0	41.0
24-25	32.80853898567602	38.0	32.0	40.0	10.0	41.0
26-27	32.83777003350649	38.0	32.0	40.0	10.0	41.0
28-29	32.684766381149494	38.0	31.0	40.0	9.5	41.0
30-31	32.44313438814079	37.5	31.0	40.0	9.0	41.0
32-33	32.20078908987581	37.0	30.5	40.0	8.0	41.0
34-35	31.87695110445273	37.0	30.0	40.0	5.0	41.0
36-37	31.579040172025557	37.0	30.0	40.0	2.0	41.0
38-39	31.380451957232893	37.0	30.0	40.0	2.0	41.0
40-41	31.187699255678645	37.0	30.0	40.0	2.0	41.0
42-43	30.955041449082554	36.0	29.5	40.0	2.0	41.0
44-45	30.671372725729903	36.0	28.5	39.0	2.0	41.0
46-47	30.417285363067172	36.0	28.0	39.0	2.0	40.0
48-49	30.122625431510667	35.5	27.5	39.0	2.0	40.0
50-51	28.979830432320206	34.0	25.5	38.0	2.0	39.5
52-53	29.434560094829934	34.5	27.5	38.5	2.0	39.5
54-55	30.088254456857385	35.5	28.0	39.0	2.0	40.0
56-57	30.03900892184268	35.5	28.0	39.0	2.0	41.0
58-59	29.797449429778446	35.0	28.0	39.0	2.0	40.0
60-61	29.4671022900545	35.0	27.0	39.0	2.0	40.0
62-63	29.082445115484575	34.5	26.0	38.5	2.0	40.0
64-65	28.664857556776695	34.0	26.0	38.0	2.0	40.0
66-67	28.275082400737105	34.0	26.0	37.5	2.0	40.0
68-69	27.83026813645263	34.0	25.0	37.0	2.0	39.5
70-71	27.498246485004657	34.0	24.5	36.5	2.0	39.0
72-73	27.035893819603345	33.0	23.5	36.0	2.0	39.0
74-75	26.598218947650416	33.0	22.0	35.5	2.0	38.5
76-77	26.187695841957044	32.5	21.5	35.0	2.0	37.5
78-79	25.733204814842992	32.0	20.0	35.0	2.0	37.0
80-81	25.294140883315094	32.0	18.0	35.0	2.0	36.0
82-83	24.825809328368155	32.0	15.5	35.0	2.0	36.0
84-85	24.432984906148665	31.0	9.0	34.5	2.0	35.5
86-87	23.980334687768018	31.0	4.5	34.0	2.0	35.0
88-89	23.455355836586122	31.0	2.0	34.0	2.0	35.0
90-91	22.95985593444614	31.0	2.0	34.0	2.0	35.0
92-93	22.42604481013531	30.0	2.0	34.0	2.0	35.0
94-95	21.805079422671405	30.0	2.0	34.0	2.0	35.0
96-97	21.173259213309485	29.0	2.0	34.0	2.0	35.0
98	20.780545493204745	29.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	166135.0
3	27737.0
4	20115.0
5	16298.0
6	15197.0
7	15417.0
8	17721.0
9	19840.0
10	22112.0
11	23458.0
12	24445.0
13	24198.0
14	22839.0
15	24083.0
16	25895.0
17	28384.0
18	28924.0
19	28545.0
20	29009.0
21	31123.0
22	33932.0
23	37891.0
24	43030.0
25	49260.0
26	57140.0
27	66357.0
28	76255.0
29	89117.0
30	103447.0
31	121561.0
32	144515.0
33	175120.0
34	216385.0
35	274031.0
36	346978.0
37	381970.0
38	225063.0
39	22294.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.603819496684366	17.703021519058808	12.596173584528808	25.096985399728013
2	16.181200727003823	19.468392276381124	39.02985509027875	25.32055190633631
3	19.76997179114063	23.320873742998593	28.60989667590703	28.299257789953746
4	13.277174736565541	15.217676886247949	36.333274586403185	35.17187379078332
5	14.565597788990935	36.64726711514241	34.12323338288094	14.66390171298571
6	34.11637652802036	34.58952587183864	17.107421810851477	14.186675789289524
7	29.80776202954601	31.064408710782022	20.63850410693609	18.489325152735884
8	26.823232497299678	34.1452304202755	19.88422248479461	19.147314597630217
9	26.97051389839854	15.6265869741487	19.249423406165896	38.15347572128686
10-11	25.857770703246157	24.978867437712584	27.566891061966526	21.596470797074733
12-13	26.568990689699625	22.36166024450857	27.258448092527193	23.810900973264605
14-15	23.601897429561667	24.05419508801134	24.768332173720793	27.575575308706195
16-17	23.797185776590535	26.970665177122843	25.537038855374877	23.695110190911745
18-19	23.711794866791234	26.250884236921827	26.957916014160798	23.079404882126138
20-21	24.57821497659556	25.63686053043553	26.424410569796763	23.360513923172142
22-23	24.101928674985803	25.509602695881817	26.427468445263084	23.961000183869295
24-25	23.912670770516048	25.56199660231982	26.467177121158986	24.05815550600515
26-27	23.754751625439415	25.752650799798992	26.56073824646293	23.93185932829866
28-29	23.80415480268737	26.04443409266054	26.404021234313607	23.747389870338484
30-31	24.064976942186156	25.752196729651565	26.145335952526633	24.03749037563565
32-33	23.767472986722694	25.850834095602725	26.086424927818808	24.295267989855773
34-35	24.250208242640884	25.676484802242477	26.15684149044808	23.91646546466856
36-37	24.156627391901804	25.469034154503255	26.26159901832151	24.112739435273433
38-39	24.051874154301192	25.6238756339934	26.274401243202767	24.049848968502644
40-41	24.23273888090627	25.519389761641364	26.316847722492536	23.931023634959832
42-43	24.065533849817363	25.518777809014537	26.20813607646497	24.207552264703125
44-45	24.047499629913922	25.519699596194062	26.229987401568124	24.202813372323895
46-47	24.021591495537326	25.62115617944189	26.143757460777973	24.21349486424281
48-49	23.884837186743333	25.67801797864516	26.076607769791877	24.36053706481964
50-51	23.791143990565025	25.767338208461414	26.28556331196038	24.155954489013173
52-53	23.96311211937288	25.519462462143238	26.306308336399102	24.211117082084776
54-55	23.6335223700733	25.442870136168505	26.515954363384896	24.4076531303733
56-57	23.655413719734465	25.49175936382504	26.41515594050274	24.437670975937753
58-59	23.646872774629582	25.304168300883216	26.40843352039706	24.640525404090134
60-61	23.498307510682533	25.299434139181127	26.711800803410053	24.490457546726287
62-63	23.50889619890796	25.289245812221367	26.742276672887712	24.459581315982966
64-65	23.32515526758604	25.25440043892128	26.574348414992883	24.846095878499796
66-67	23.382679682840195	25.23611576581079	26.463110933415912	24.918093617933103
68-69	23.556742200184743	25.09947634236544	26.45524703121656	24.888534426233257
70-71	23.364782260031635	24.950493434067795	26.62763899732174	25.057085308578824
72-73	23.365722464915038	24.921947315375913	26.63892692689557	25.073403292813474
74-75	23.39813014601302	25.14860641726581	26.521915001892037	24.93134843482913
76-77	23.364321047419097	24.84234301827164	26.69144444197975	25.101891492329514
78-79	23.27036588017291	24.92005041223764	26.72510797903981	25.084475728549638
80-81	23.426324625928288	24.85266164968056	26.736641507784203	24.98437221660695
82-83	23.397836644137897	24.7758419009057	26.825811698877544	25.00050975607886
84-85	23.342194710284847	24.757497493209605	26.79852321621493	25.101784580290616
86-87	23.38307492096599	24.73300179523785	26.84874676675331	25.03517651704285
88-89	23.348547238490198	24.43745753519072	26.83291333556549	25.38108189075359
90-91	23.3052345389882	24.50435315703193	26.928251417351518	25.262160886628354
92-93	23.4945178305691	24.317646604010662	26.96005275906038	25.227782806359862
94-95	23.37778095334574	24.217207543648698	27.09092798415435	25.31408351885121
96-97	23.61693617190653	23.961904836477967	27.09140209607574	25.329756895539763
98	23.527423616487283	23.856600319128493	26.97895063426054	25.637025430123682
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	104.0
1	73.5
2	75.5
3	129.0
4	141.5
5	176.5
6	257.5
7	451.5
8	706.0
9	803.0
10	820.5
11	877.0
12	1013.5
13	1201.5
14	1332.0
15	1486.0
16	1600.5
17	1674.5
18	1664.0
19	1602.5
20	1672.5
21	1789.5
22	2030.5
23	2364.5
24	2779.5
25	3501.0
26	4561.0
27	5980.5
28	7850.0
29	10766.5
30	15150.5
31	21127.0
32	27891.5
33	34129.5
34	41215.5
35	48869.0
36	57212.5
37	65306.0
38	71585.0
39	78763.5
40	86884.5
41	93875.5
42	101394.5
43	109322.0
44	116280.5
45	123280.5
46	130314.0
47	136742.0
48	141812.5
49	142914.0
50	141817.0
51	142216.5
52	141455.5
53	138801.5
54	133448.5
55	125749.0
56	115891.0
57	104114.5
58	93654.0
59	81293.0
60	66618.5
61	54005.5
62	43975.5
63	33897.5
64	24888.0
65	18958.5
66	14171.0
67	10306.5
68	7607.0
69	5664.0
70	4073.0
71	2945.0
72	2296.0
73	1886.0
74	1492.0
75	1088.0
76	722.5
77	425.0
78	249.5
79	155.0
80	79.0
81	48.0
82	34.5
83	23.5
84	20.5
85	12.5
86	11.5
87	11.0
88	6.0
89	5.5
90	7.5
91	4.0
92	1.5
93	1.5
94	0.5
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.38041863280775023
2	0.02389605120192261
3	0.017783863955716555
4	0.01573563099555176
5	0.021620236801739502
6	0.022205446218929444
7	0.013492328229656984
8	0.00871311798927246
9	0.0
10-11	0.0
12-13	0.0046329078860870366
14-15	0.0026659540116430666
16-17	0.005201861486132813
18-19	0.036965728185831305
20-21	0.07693878254333314
22-23	0.09711225161924195
24-25	0.06372280320512695
26-27	0.07375264238307679
28-29	0.05660275529598267
30-31	0.05179103342130982
32-33	0.018694189715789795
34-35	0.3344634377411957
36-37	0.512285821481217
38-39	0.4673384870776007
40-41	0.4765392795811982
42-43	0.4288609678973621
44-45	0.40137238110657897
46-47	0.3999256133807483
48-49	0.3908060999628717
50-51	0.38696972711684874
52-53	0.4482216461160626
54-55	0.4488881346189734
56-57	0.4191074776108631
58-59	0.404038335118222
60-61	0.4186848263651148
62-63	0.3997142877578742
64-65	0.44766894833316095
66-67	0.46816753375195314
68-69	0.41148349937024964
70-71	0.3683405606696356
72-73	0.3545393719142395
74-75	0.33623532180990967
76-77	0.33506490297552977
78-79	0.3347560424497907
80-81	0.3500852780167383
82-83	0.3456149283020929
84-85	0.3461676260849945
86-87	0.33199255353528256
88-89	0.3745340270015625
90-91	0.3531576274569855
92-93	0.3435341837076398
94-95	0.34961385931955746
96-97	0.34985769657672
98	0.3445908118220105
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3075822.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.17455420683454
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.78722069266321	33.86530133924035
2	11.353904688972577	10.712307844183394
3	5.467688655862549	7.738073246462463
4	3.1470779917608875	5.9384800526184
5	2.0194588957168964	4.763353657223547
6	1.3747379571636673	3.891159016824624
7	0.9825930920833172	3.2447373760021927
8	0.7011745025324647	2.6462075662534397
9	0.537424584480776	2.2817488673406507
>10	2.5246171236739436	19.672580126855628
>50	0.0764660010214493	2.426138217860156
>100	0.026109805239153336	2.172013613146385
>500	0.0011098246030642673	0.37800657204690613
>1k	4.1618422601992754E-4	0.2698925039419887
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	3.251163428833008E-5	0.0
10-11	0.0	0.0	0.0	8.12790857208252E-5	0.0
12-13	0.0	0.0	0.0	9.753490286499024E-5	0.0
14-15	0.0	0.0	0.0	9.753490286499024E-5	0.0
16-17	0.0	0.0	0.0	9.753490286499024E-5	0.0
18-19	0.0	0.0	0.0	1.7881398858581542E-4	0.0
20-21	0.0	0.0	0.0	2.763488914508057E-4	0.0
22-23	0.0	0.0	0.0	4.06395428604126E-4	1.625581714416504E-5
24-25	0.0	0.0	0.0	7.965350400640869E-4	3.251163428833008E-5
26-27	0.0	0.0	0.0	0.0019344422401556397	3.251163428833008E-5
28-29	0.0	0.0	0.0	0.008290466743524169	3.251163428833008E-5
30-31	0.0	0.0	0.0	0.033080587888375854	3.251163428833008E-5
32-33	0.0	0.0	0.0	0.0841563653553424	3.251163428833008E-5
34-35	0.0	0.0	0.0	0.15324358821804385	3.251163428833008E-5
36-37	0.0	0.0	0.0	0.268009657255849	3.251163428833008E-5
38-39	0.0	0.0	0.0	0.4528545540021497	3.251163428833008E-5
40-41	0.0	0.0	0.0	0.6637737814476911	3.251163428833008E-5
42-43	0.0	0.0	0.0	0.8849829411454889	3.251163428833008E-5
44-45	0.0	3.251163428833008E-5	0.0	1.151318249235489	3.251163428833008E-5
46-47	0.0	3.251163428833008E-5	0.0	1.4719479865870002	3.251163428833008E-5
48-49	0.0	3.251163428833008E-5	0.0	1.8090448667055505	3.251163428833008E-5
50-51	0.0	3.251163428833008E-5	0.0	2.1684122163116073	3.251163428833008E-5
52-53	0.0	3.251163428833008E-5	0.0	2.5338917531638696	3.251163428833008E-5
54-55	0.0	3.251163428833008E-5	0.0	2.9463343457456252	3.251163428833008E-5
56-57	0.0	3.251163428833008E-5	0.0	3.426027253852791	3.251163428833008E-5
58-59	0.0	3.251163428833008E-5	0.0	3.9307703761791153	3.251163428833008E-5
60-61	0.0	3.251163428833008E-5	0.0	4.469211807445294	3.251163428833008E-5
62-63	0.0	3.251163428833008E-5	0.0	5.013505332883373	3.251163428833008E-5
64-65	0.0	3.251163428833008E-5	0.0	5.594000563101506	3.251163428833008E-5
66-67	0.0	3.251163428833008E-5	0.0	6.267787927910003	3.251163428833008E-5
68-69	0.0	3.251163428833008E-5	0.0	7.002030676677649	3.251163428833008E-5
70-71	0.0	3.251163428833008E-5	0.0	7.763388128441763	3.251163428833008E-5
72-73	0.0	3.251163428833008E-5	0.0	8.534352768138078	3.251163428833008E-5
74-75	0.0	3.251163428833008E-5	0.0	9.312648781366413	6.502326857666016E-5
76-77	0.0	3.251163428833008E-5	0.0	10.172045066326984	9.753490286499024E-5
78-79	1.625581714416504E-5	3.251163428833008E-5	0.0	11.10763236624226	9.753490286499024E-5
80-81	3.251163428833008E-5	3.251163428833008E-5	0.0	12.081908510960648	9.753490286499024E-5
82-83	3.251163428833008E-5	3.251163428833008E-5	0.0	13.061614098605187	9.753490286499024E-5
84-85	3.251163428833008E-5	3.251163428833008E-5	0.0	14.05523466572513	9.753490286499024E-5
86	3.251163428833008E-5	3.251163428833008E-5	0.0	14.815551745192018	9.753490286499024E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6930	0.0	30.480375	1
GTACATG	9355	0.0	26.858994	1
TCAACGC	8045	0.0	25.710209	4
TACATGG	9425	0.0	25.645588	2
CAACGCA	8030	0.0	25.529785	5
ATCAACG	8200	0.0	25.000507	3
AACGCAG	8310	0.0	24.830698	6
TATCAAC	8410	0.0	24.541431	2
ACATGGG	9835	0.0	23.455719	3
GGTATCA	4785	0.0	23.370352	1
ACGCAGA	9350	0.0	21.527922	7
CATGGGG	6575	0.0	21.134989	4
CGCAGAG	9505	0.0	20.983225	8
GAGTACA	6680	0.0	18.738436	1
AGTACAT	6795	0.0	18.08959	2
AGAGTAC	7290	0.0	17.73633	10-11
GCAGAGT	10700	0.0	17.611368	9
TAAGGCG	655	0.0	17.504667	5
GCGTACG	160	0.0015103123	17.19358	9
TCGCGTA	165	0.0018556323	16.672564	9
>>END_MODULE