##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920884_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3075822
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.16992335707333	31.0	30.0	34.0	26.0	34.0
2	30.18080825223306	31.0	30.0	34.0	26.0	34.0
3	30.31900155470635	31.0	30.0	34.0	26.0	34.0
4	33.95635053003718	35.0	35.0	37.0	30.0	37.0
5	33.46910679486654	35.0	35.0	37.0	28.0	37.0
6	33.40962903575044	35.0	35.0	37.0	28.0	37.0
7	33.22055047398712	35.0	33.0	37.0	27.0	37.0
8	33.15036435788547	35.0	33.0	37.0	27.0	37.0
9	34.47969453368888	38.0	34.0	39.0	27.0	39.0
10-11	34.276933125518966	37.0	34.0	39.0	27.0	39.0
12-13	34.100644803242844	37.0	34.0	39.0	26.0	39.0
14-15	34.98897562992917	38.0	34.0	40.0	25.0	41.0
16-17	34.86496877907759	38.0	33.5	40.0	25.0	41.0
18-19	34.77311918570061	38.0	33.5	40.0	25.0	41.0
20-21	34.65582680012042	38.0	34.0	40.0	24.5	41.0
22-23	34.50600652443477	38.0	33.0	40.0	24.0	41.0
24-25	34.32807051253291	38.0	33.0	40.0	23.0	41.0
26-27	34.14363314912241	38.0	33.0	40.0	22.0	41.0
28-29	34.085814621262216	38.0	33.0	40.0	22.5	41.0
30-31	34.07127102933785	38.0	33.0	40.0	22.0	41.0
32-33	33.92121081779115	38.0	33.0	40.0	20.5	41.0
34-35	33.751494397270065	38.0	33.0	40.0	19.0	41.0
36-37	33.56049179698955	38.0	32.5	40.0	18.0	41.0
38-39	33.37625372989724	38.0	32.0	40.0	17.0	41.0
40-41	33.15774043491463	38.0	32.0	40.0	15.5	41.0
42-43	33.1795227422133	38.0	32.0	40.0	15.5	41.0
44-45	33.0319124448684	38.0	32.0	40.0	15.0	41.0
46-47	32.77893584219113	37.5	31.0	40.0	11.0	41.0
48-49	32.51522812438431	37.0	31.0	40.0	9.0	41.0
50-51	32.24992847440457	37.0	31.0	40.0	8.0	41.0
52-53	31.991516901823317	37.0	30.5	40.0	7.5	41.0
54-55	31.707562563763442	36.0	30.0	40.0	4.0	41.0
56-57	31.38142112905103	36.0	30.0	39.0	2.0	41.0
58-59	31.045579685690527	35.5	29.0	39.0	2.0	40.0
60-61	30.710568264353398	35.0	29.0	39.0	2.0	40.0
62-63	30.38286805933503	35.0	28.5	39.0	2.0	40.0
64-65	29.97528628119573	35.0	28.0	38.0	2.0	40.0
66-67	29.53553570395166	34.0	28.0	38.0	2.0	40.0
68-69	29.07787186644741	34.0	27.0	37.0	2.0	39.5
70-71	28.64733915031494	34.0	26.0	36.5	2.0	39.0
72-73	28.200188762548677	34.0	26.0	36.0	2.0	39.0
74-75	27.748591108328114	33.0	26.0	35.5	2.0	38.5
76-77	26.305553604857497	31.5	24.0	34.0	2.0	36.0
78-79	27.14065378295623	33.0	25.5	35.0	2.0	37.0
80-81	27.167497989155418	33.0	26.0	35.0	2.0	36.5
82-83	26.824435711819476	33.0	25.0	35.0	2.0	36.0
84-85	26.5010393969482	33.0	24.5	35.0	2.0	36.0
86-87	25.968237921440185	32.0	23.0	35.0	2.0	35.0
88-89	25.598963138959277	32.0	21.5	35.0	2.0	35.0
90-91	25.232372029330698	32.0	20.0	34.5	2.0	35.0
92-93	24.915076847749965	32.0	18.5	34.0	2.0	35.0
94-95	24.53058662042212	32.0	15.0	34.0	2.0	35.0
96-97	23.993720052720867	31.0	4.5	34.0	2.0	35.0
98-99	23.38406107375524	31.0	2.0	34.0	2.0	35.0
100-101	21.193651973358666	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	77413.0
3	34358.0
4	18374.0
5	12942.0
6	11099.0
7	10856.0
8	12456.0
9	14290.0
10	16137.0
11	18284.0
12	20322.0
13	21717.0
14	23671.0
15	25314.0
16	26563.0
17	27689.0
18	26755.0
19	25938.0
20	26096.0
21	27154.0
22	29882.0
23	33254.0
24	38474.0
25	44872.0
26	52846.0
27	63642.0
28	74561.0
29	88269.0
30	104046.0
31	122476.0
32	146605.0
33	179197.0
34	223723.0
35	292287.0
36	386622.0
37	449905.0
38	251238.0
39	16494.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.57746704134844	19.056848197530734	13.253298145299174	25.112386615821652
2	16.215535229281798	20.5292764015603	37.89770669434057	25.357481674817333
3	19.52373024755466	24.164136407059537	28.95509003258719	27.357043312798616
4	13.692939851733927	16.264851238059748	35.804380349071366	34.23782856113496
5	14.506929187774926	37.03271510427352	34.050480343268156	14.409875364683398
6	34.04183993654718	35.13322456264713	16.799538098869366	14.025397401936326
7	29.622371584329393	31.28797902737225	20.59609328808191	18.493556100216445
8	26.793531953678762	34.510721755194055	19.752475972689023	18.943270318438163
9	26.682457202763505	15.56748256529468	19.21815683746492	38.5319033944769
10-11	25.826523617610892	25.194950015070017	27.544391448042948	21.434134919276143
12-13	26.500259086245777	22.411220647741654	27.357245431489265	23.731274834523308
14-15	23.50160313658502	24.101747948618254	24.756689581947615	27.639959332849106
16-17	23.76365501997867	27.19545783722832	25.437588531117083	23.603298611675925
18-19	23.726433104905944	26.377108390025334	26.87429302771795	23.022165477350775
20-21	24.495457675848886	25.658614641952	26.357432491216603	23.488495190982515
22-23	24.087961160531037	25.637355444884275	26.381026956506037	23.89365643807865
24-25	23.96052574222834	25.718595053958065	26.368860143640937	23.95201906017266
26-27	23.825245712735292	25.867351071779577	26.467094777723954	23.84030843776118
28-29	23.799614682399195	26.085338074969922	26.329873432583973	23.78517381004691
30-31	24.12130063359523	25.76196220541935	26.13478292611503	23.981954234870386
32-33	23.843629786564925	25.859650303858423	26.14418270433916	24.15253720523749
34-35	24.168414371048357	25.75961527361661	26.223809025975175	23.848161329359858
36-37	24.171028845500338	25.529371823484514	26.264825906870882	24.034773424144266
38-39	23.986046837602444	25.653245507069677	26.311487179003525	24.049220476324358
40-41	24.148957481044224	25.57163260979001	26.342302504152777	23.937107405012988
42-43	24.024416652955903	25.580599471555633	26.258225646915147	24.136758228573324
44-45	23.954679895870427	25.53925753207708	26.30383916826422	24.202223403788274
46-47	23.96157847681807	25.602749068472058	26.196936568289118	24.238735886420752
48-49	23.834922319484086	25.661232184464154	26.131961609418298	24.371883886633462
50-51	23.72382308015598	25.803945732961886	26.30453956829269	24.167691618589444
52-53	23.922505658188513	25.54643258570355	26.29479934361437	24.236262412493566
54-55	23.609297008136394	25.42651606712663	26.41403297933532	24.55015394540165
56-57	23.600037715114333	25.595134750251162	26.199763305144536	24.60506422948997
58-59	23.497094091734397	25.48170483202857	26.270285314007317	24.75091576222971
60-61	23.400513898561048	25.56103086041669	26.456963231196955	24.581492009825308
62-63	23.38693079119329	25.563343942924753	26.40081235069593	24.64891291518602
64-65	23.113249876609682	25.546623982395666	26.247185107585302	25.09294103340935
66-67	23.220546092202294	25.512008193562174	26.09975772529125	25.167687988944287
68-69	23.45033255999853	25.34701223164119	26.075521927775767	25.127133280584513
70-71	23.17521194435994	25.190636515158488	26.156749318774953	25.47740222170662
72-73	23.250134333581833	25.12387637927737	26.03175021369773	25.594239073443063
74-75	23.216915900942332	25.34181618743715	25.884348766481097	25.556919145139425
76-77	23.16706950137146	24.939819232814994	26.17050207023143	25.722609195582113
78-79	23.09326073531201	25.062439764661633	26.106882300230172	25.737417199796187
80-81	23.226692381068048	25.00735289455291	26.031945623861198	25.734009100517845
82-83	23.122587156625542	24.98192958537381	26.06722747002165	25.828255787979003
84-85	23.017904601246293	24.866925480707874	26.02885734830984	26.08631256973599
86-87	22.954323867875846	24.841553307292543	26.0826905443108	26.12143228052081
88-89	22.899668422289757	24.658399387977326	25.948969648968617	26.492962540764296
90-91	22.836781775560638	24.666475638907986	25.965806683168076	26.5309359023633
92-93	22.952607969503518	24.53460984943274	25.979564823463814	26.53321735759993
94-95	22.684067285456003	24.411216496829596	26.130514069388983	26.774202148325422
96-97	22.844533428617783	24.15338867244334	26.136414991954688	26.865662906984195
98-99	22.65997511802519	24.1552020087651	26.02102499235545	27.163797880854258
100-101	22.773315073935766	23.97862714105336	26.06537130936885	27.182686475642026
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	127.0
1	78.0
2	67.0
3	108.0
4	88.5
5	91.0
6	145.0
7	275.5
8	421.5
9	453.5
10	401.0
11	345.5
12	341.5
13	376.0
14	428.5
15	470.5
16	521.5
17	598.5
18	691.0
19	794.0
20	935.0
21	1126.5
22	1357.0
23	1659.5
24	2056.0
25	2581.5
26	3415.0
27	4643.0
28	6135.0
29	8192.5
30	11493.5
31	16323.0
32	22754.5
33	29114.0
34	35133.5
35	41593.0
36	49023.5
37	56750.5
38	64017.0
39	70212.0
40	76868.0
41	85052.0
42	92388.0
43	99383.5
44	106407.0
45	113792.5
46	120526.5
47	127363.5
48	135160.5
49	139234.5
50	140176.0
51	141360.5
52	142046.0
53	141047.5
54	137623.5
55	130463.0
56	119826.0
57	107602.5
58	96484.0
59	84127.5
60	69059.0
61	55927.0
62	45337.5
63	34723.5
64	25212.0
65	19058.5
66	14461.0
67	10516.0
68	7700.0
69	5833.0
70	4307.0
71	3043.0
72	2230.0
73	1919.5
74	1557.5
75	1083.0
76	717.0
77	415.5
78	251.0
79	131.5
80	56.5
81	32.5
82	17.5
83	7.0
84	4.0
85	3.5
86	3.5
87	2.0
88	1.0
89	1.5
90	1.0
91	1.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18983543260955935
2	0.0
3	0.012354421029565429
4	0.013167211886773684
5	0.0067949315662609875
6	0.10738592805435425
7	0.14272607452576905
8	0.1234466753927893
9	0.1418157487656958
10-11	0.11366067347200196
12-13	0.11333555712911866
14-15	0.11585520878646424
16-17	0.06645378048534668
18-19	0.07856436425774964
20-21	0.03672189092866882
22-23	0.08311599305811584
24-25	0.05748056942176758
26-27	0.06531587328525512
28-29	0.03951789147746521
30-31	0.024432493167680057
32-33	0.030967331659634396
34-35	0.019425701487277222
36-37	0.023245818516156005
38-39	0.03132495963680603
40-41	0.025033958402014164
42-43	0.012354421029565429
44-45	0.026236888870682377
46-47	0.03364954148842163
48-49	0.008973211063579102
50-51	0.015426770469812622
52-53	0.006209722149071045
54-55	0.01238693266385376
56-57	0.004291535726059571
58-59	0.0034299774174188233
60-61	0.02280691145326355
62-63	0.009851025189364013
64-65	0.007022513006279298
66-67	0.03943661239174438
68-69	0.08997594789295349
70-71	0.021571469350307005
72-73	0.04506112512362549
74-75	0.08591199360691223
76-77	0.24598302502550537
78-79	0.079978620349292
80-81	0.016337096229885866
82-83	0.011476606903780518
84-85	0.012468211749574586
86-87	0.2624014003411121
88-89	0.6200781449641755
90-91	0.2667579593357483
92-93	0.35154830155971317
94-95	0.0010078606629382325
96-97	0.0038688844803112794
98-99	0.0032999308802655032
100-101	0.004730442788952027
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3075822.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.80556551577446
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.79883055938252	30.13771679477697
2	12.313781151954203	10.788242939976769
3	5.9379045277936955	7.803397974560417
4	3.4783487614567767	6.094841382268311
5	2.2633807322216017	4.957433647623747
6	1.5563653505515922	4.090647859804144
7	1.1139924439767264	3.4159348292090135
8	0.8517083583486462	2.984765303357946
9	0.6024050822913033	2.3749825769412474
>10	2.957095177142311	21.42665811145402
>50	0.09221825207655268	2.707439107164407
>100	0.03195763133126312	2.4096385677924195
>500	0.0013413143154490294	0.4142044336346556
>1k	6.706571575050302E-4	0.3940964714358787
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	3.251163428833008E-5	0.0
10-11	0.0	0.0	0.0	4.876745143249512E-5	0.0
12-13	0.0	0.0	0.0	6.502326857666016E-5	0.0
14-15	0.0	0.0	0.0	6.502326857666016E-5	6.502326857666016E-5
16-17	0.0	0.0	0.0	6.502326857666016E-5	6.502326857666016E-5
18-19	0.0	0.0	0.0	1.1379072000915528E-4	6.502326857666016E-5
20-21	0.0	0.0	0.0	1.4630235429748535E-4	6.502326857666016E-5
22-23	0.0	0.0	0.0	2.763488914508057E-4	6.502326857666016E-5
24-25	0.0	0.0	0.0	6.827443200549317E-4	6.502326857666016E-5
26-27	0.0	0.0	0.0	0.0018694189715789795	6.502326857666016E-5
28-29	0.0	0.0	0.0	0.008225443474947509	9.753490286499024E-5
30-31	3.251163428833008E-5	0.0	0.0	0.03306433207123169	9.753490286499024E-5
32-33	6.502326857666016E-5	0.0	0.0	0.08485536549254151	9.753490286499024E-5
34-35	6.502326857666016E-5	0.0	0.0	0.1548854257496045	9.753490286499024E-5
36-37	6.502326857666016E-5	0.0	0.0	0.2704967972789063	9.753490286499024E-5
38-39	6.502326857666016E-5	0.0	0.0	0.4551628800366211	1.300465371533203E-4
40-41	6.502326857666016E-5	0.0	0.0	0.6683416660652014	1.300465371533203E-4
42-43	6.502326857666016E-5	0.0	0.0	0.891989198334624	1.6255817144165038E-4
44-45	6.502326857666016E-5	0.0	0.0	1.1599663439561847	1.6255817144165038E-4
46-47	6.502326857666016E-5	0.0	0.0	1.4813601047134717	1.6255817144165038E-4
48-49	6.502326857666016E-5	0.0	0.0	1.817481635803372	1.6255817144165038E-4
50-51	6.502326857666016E-5	0.0	0.0	2.174150519763497	1.6255817144165038E-4
52-53	6.502326857666016E-5	0.0	0.0	2.536395149004071	1.6255817144165038E-4
54-55	6.502326857666016E-5	0.0	0.0	2.945098903642669	1.6255817144165038E-4
56-57	6.502326857666016E-5	0.0	0.0	3.419492415360837	1.6255817144165038E-4
58-59	6.502326857666016E-5	0.0	0.0	3.9196513972525064	1.6255817144165038E-4
60-61	6.502326857666016E-5	0.0	0.0	4.453004757752562	1.6255817144165038E-4
62-63	6.502326857666016E-5	0.0	0.0	4.993998352310374	1.6255817144165038E-4
64-65	6.502326857666016E-5	0.0	0.0	5.572835489179803	1.6255817144165038E-4
66-67	6.502326857666016E-5	0.0	0.0	6.239356503724858	2.2758144001831055E-4
68-69	6.502326857666016E-5	0.0	0.0	6.964122111097456	2.2758144001831055E-4
70-71	6.502326857666016E-5	0.0	0.0	7.717985631158109	2.2758144001831055E-4
72-73	6.502326857666016E-5	0.0	0.0	8.486934549528549	2.2758144001831055E-4
74-75	6.502326857666016E-5	0.0	0.0	9.266368469956975	2.2758144001831055E-4
76-77	6.502326857666016E-5	0.0	0.0	10.127048964471935	2.2758144001831055E-4
78-79	6.502326857666016E-5	0.0	0.0	11.061595892089985	2.2758144001831055E-4
80-81	6.502326857666016E-5	0.0	0.0	12.032490826842386	2.438372571624756E-4
82-83	6.502326857666016E-5	0.0	0.0	13.011383623629715	2.600930743066406E-4
84-85	6.502326857666016E-5	0.0	0.0	14.004906655846796	2.600930743066406E-4
86-87	6.502326857666016E-5	0.0	0.0	15.030973833986494	2.600930743066406E-4
88-89	6.502326857666016E-5	0.0	0.0	16.109563557318985	2.600930743066406E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6535	0.0	32.522648	1
TCAACGC	7835	0.0	26.598423	4
CAACGCA	7900	0.0	26.313488	5
GGTATCA	4645	0.0	25.999916	1
ATCAACG	8020	0.0	25.984873	3
GTACATG	9530	0.0	25.494822	1
AACGCAG	8250	0.0	25.369743	6
TATCAAC	8355	0.0	25.281836	2
TACATGG	9650	0.0	24.594484	2
ACATGGG	10060	0.0	23.027788	3
ACGCAGA	9675	0.0	21.387838	7
CGCAGAG	9825	0.0	20.818424	8
CATGGGG	6680	0.0	19.827051	4
GTATAGG	620	0.0	19.172215	1
TAAGGCG	800	0.0	18.390905	5
GCAGAGT	10880	0.0	18.014585	9
AGAGTAC	8010	0.0	17.921225	10-11
GTGTAAG	1340	0.0	17.741453	1
CTAGACG	270	4.9110713E-7	17.581934	4
TATACCG	135	0.00944914	17.577925	5
>>END_MODULE