FastQCFastQC Report
Mon 15 Aug 2016
SRR2920883_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920883_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2793067
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29250.047.0423971
GTATCAA54750.043.287971
TCAACGC69300.033.626384
ATCAACG70900.032.867533
TATCAAC71700.032.3734322
CAACGCA71900.032.3466035
AACGCAG74900.031.4134166
ACGCAGA88400.025.9911927
GTACATG112700.025.1944791
CGCAGAG91500.024.7087658
TACATGG114500.024.3187142
ACATGGG120100.022.344083
GCAGAGT103300.021.4863419
GTATAGG6700.020.6058521
TATACCG2101.2039582E-619.6595175
CATGGGG84650.019.616944
GTGGTAT16550.018.6303461
GAGTACA81950.018.2506581
AGAGTAC88500.018.13635410-11
CAGAGTA95650.017.78746810-11
Produced by FastQC (version 0.11.5)