##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920883_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2793067
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.09411303058609	31.0	30.0	33.0	25.0	34.0
2	29.16442033076901	31.0	30.0	34.0	23.0	34.0
3	29.22694944303162	31.0	30.0	34.0	23.0	34.0
4	32.762605050290595	35.0	33.0	37.0	26.0	37.0
5	32.72026628791934	35.0	33.0	37.0	26.0	37.0
6	32.83659396641756	35.0	33.0	37.0	26.0	37.0
7	32.69198698062023	35.0	33.0	37.0	25.0	37.0
8	32.636103967430785	35.0	33.0	37.0	25.0	37.0
9	33.960849131080636	37.0	34.0	39.0	25.0	39.0
10-11	33.786013905144415	37.0	33.5	39.0	25.0	39.0
12-13	33.621488492757244	37.0	33.0	39.0	23.0	39.0
14-15	34.44618210017876	38.0	33.0	40.0	23.5	41.0
16-17	34.34367149087365	38.0	33.0	40.0	22.5	41.0
18-19	34.23168062205454	38.0	33.0	40.0	21.5	41.0
20-21	34.13716999986037	38.0	33.0	40.0	19.0	41.0
22-23	33.92087640575754	38.0	33.0	40.0	18.5	41.0
24-25	33.72449515174537	38.0	33.0	40.0	18.0	41.0
26-27	33.76038920656039	38.0	33.0	40.0	18.0	41.0
28-29	33.614450709560494	38.0	33.0	40.0	17.0	41.0
30-31	33.36877507771922	38.0	32.5	40.0	16.0	41.0
32-33	33.12466170700524	38.0	32.0	40.0	15.0	41.0
34-35	32.80554691312453	38.0	31.5	40.0	10.5	41.0
36-37	32.49595212001717	37.5	31.0	40.0	9.0	41.0
38-39	32.29885946165989	37.0	31.0	40.0	8.0	41.0
40-41	32.089443790643045	37.0	30.5	40.0	7.5	41.0
42-43	31.848741186659684	37.0	30.0	40.0	4.0	41.0
44-45	31.565075954139303	37.0	30.0	40.0	2.0	41.0
46-47	31.29582820605449	36.0	30.0	39.5	2.0	41.0
48-49	30.979774921260393	36.0	29.0	39.0	2.0	40.0
50-51	29.81232960039985	34.5	27.5	38.0	2.0	39.5
52-53	30.2765425963645	35.5	28.5	38.5	2.0	39.5
54-55	30.92814959326074	36.0	30.0	39.0	2.0	40.0
56-57	30.865474405017853	36.0	29.0	39.0	2.0	41.0
58-59	30.609355951719024	35.5	29.0	39.0	2.0	40.5
60-61	30.269488522831715	35.0	28.5	39.0	2.0	40.0
62-63	29.86396334208954	35.0	28.0	38.5	2.0	40.0
64-65	29.436952282204473	34.5	28.0	38.0	2.0	40.0
66-67	29.03896093434207	34.0	27.5	37.5	2.0	40.0
68-69	28.584906126491056	34.0	26.0	37.0	2.0	39.5
70-71	28.25255319689789	34.0	26.0	36.0	2.0	39.0
72-73	27.79884048610363	34.0	26.0	36.0	2.0	39.0
74-75	27.370563971433555	33.0	25.5	35.0	2.0	38.0
76-77	26.961287538036146	33.0	25.0	35.0	2.0	37.0
78-79	26.52385871874896	33.0	24.0	35.0	2.0	37.0
80-81	26.1013097788202	32.5	23.0	35.0	2.0	36.0
82-83	25.649004660468222	32.0	20.5	35.0	2.0	36.0
84-85	25.273254633705527	32.0	19.5	35.0	2.0	35.0
86-87	24.837640128217476	32.0	18.0	34.0	2.0	35.0
88-89	24.322389867482592	31.0	11.5	34.0	2.0	35.0
90-91	23.841201267280734	31.0	4.5	34.0	2.0	35.0
92-93	23.327150762942672	31.0	2.0	34.0	2.0	35.0
94-95	22.69423898531614	30.5	2.0	34.0	2.0	35.0
96-97	22.050607092490083	30.0	2.0	34.0	2.0	35.0
98	21.655397453766774	30.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	125738.0
3	20798.0
4	14988.0
5	12467.0
6	11682.0
7	11901.0
8	13450.0
9	15530.0
10	16796.0
11	18408.0
12	19749.0
13	19952.0
14	18411.0
15	19429.0
16	20892.0
17	22986.0
18	23412.0
19	23275.0
20	23555.0
21	25279.0
22	28012.0
23	31046.0
24	35201.0
25	41129.0
26	47933.0
27	56288.0
28	65813.0
29	77389.0
30	91752.0
31	110182.0
32	134327.0
33	165957.0
34	211882.0
35	272650.0
36	349188.0
37	371824.0
38	204613.0
39	19182.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.99416943140818	17.74137684486764	13.293179010815367	24.97127471290881
2	16.178572362208364	18.901727831993238	39.05360830723029	25.866091498568107
3	19.49204860004512	23.05508506440258	28.511990660999288	28.940875674553013
4	13.707727890094395	15.077716477998862	35.41223017126021	35.802325460646536
5	15.040735855968643	36.361930497478426	33.294753846280564	15.302579800272373
6	34.391876962656134	33.8493790226729	16.81632600441261	14.942418010258365
7	30.3187411488145	30.975416307844906	20.02320331005244	18.682639233288157
8	27.630379929942706	33.576405809534826	19.393617360287333	19.399596900235135
9	27.37295596561056	15.494794790099913	18.946090444661728	38.1861587996278
10-11	26.431079680895476	24.633664224189474	26.637967022619584	22.297289072295463
12-13	26.824762145164627	21.946072810567387	26.887760445338365	24.34140459892962
14-15	24.08114170098323	23.44850438982875	24.26313301101454	28.207220898173478
16-17	24.19940764759042	26.590371695408265	25.058486923419547	24.15173373358177
18-19	24.326226872275488	25.68160764692051	26.4653281786077	23.5268373021963
20-21	24.994213750850918	24.826018415678405	26.324836086130915	23.85493174733976
22-23	24.684684555550042	24.605059488561242	26.27353073923561	24.43672521665311
24-25	24.513904711137098	24.882318297301595	26.15002070624784	24.453756285313467
26-27	24.07104335136998	25.236845207151553	26.072749446558056	24.619361994920407
28-29	24.15932879200938	25.326642364201334	26.024032252200197	24.48999659158909
30-31	24.441328525369507	25.08076455078289	25.894678512492312	24.583228411355286
32-33	24.119939375003916	25.204260221963402	25.727217955455128	24.948582447577554
34-35	24.604772379022734	25.24504139436603	25.866419286328558	24.283766940282682
36-37	24.720300178735204	24.957636500761474	25.794438146053484	24.52762517444984
38-39	24.452423969523267	25.051182009103538	25.770238221305846	24.72615580006735
40-41	24.64565024880766	24.975667645115323	25.916039215862558	24.462642890214465
42-43	24.523638185723776	24.979457402236648	25.692535362743097	24.804369049296486
44-45	24.30594025337199	25.06650543049071	25.871380608714478	24.756173707422825
46-47	24.496187740613276	25.078349234367796	25.688486822127743	24.736976202891185
48-49	24.429179361451286	25.209156270987044	25.4964575577073	24.865206809854374
50-51	24.346179481974012	25.35776203574117	25.70550813581429	24.590550346470526
52-53	24.557209136780266	25.016043472868105	25.768046435293122	24.658700955058503
54-55	24.203617166766016	24.982024178199357	25.966308276715488	24.848050378319144
56-57	24.10200137909643	25.161087771742107	25.875939188209284	24.86097166095218
58-59	24.192763567861046	24.849818984979024	25.893826802010462	25.063590645149464
60-61	24.08191850525577	24.904208982116312	26.14301808783858	24.87085442478934
62-63	24.000539037548457	25.02855551412952	26.10693606565477	24.863969382667246
64-65	23.934667223437884	24.87244803952956	25.99455296549882	25.198331771533734
66-67	23.98584882762172	24.895099589316104	26.011292458641243	25.10775912442093
68-69	24.145423820368155	24.62753551595691	26.068317935313402	25.158722728361532
70-71	23.955274793059605	24.65294979649948	26.092560133154613	25.299215277286297
72-73	23.882872106556317	24.742625400520136	26.058037702774545	25.316464790149002
74-75	23.88497401771371	24.99481934508621	25.96361079257017	25.156595844629916
76-77	23.95603969371011	24.591665556322123	26.15667673818004	25.29561801178773
78-79	23.90217326112713	24.6695108124347	26.112497589275808	25.31581833716236
80-81	23.985181864648293	24.706045679971524	26.03256225437955	25.276210201000637
82-83	23.943153557374437	24.5601681242125	26.13911624879855	25.357562069614513
84-85	23.81079034724821	24.729716915734176	26.048411022808658	25.411081714208954
86-87	23.895830583739823	24.715115177666387	25.97440655916093	25.41464767943286
88-89	23.736249365203317	24.496173076429073	26.053528966578842	25.714048591788774
90-91	23.77382740280187	24.462854090642463	26.171447742703023	25.591870763852643
92-93	23.895506455779312	24.326963276694112	26.240889571477627	25.536640696048952
94-95	23.75282079372848	24.217366121739754	26.279228272143172	25.750584812388595
96-97	23.93876425783811	23.960513578508582	26.318186634674273	25.78253552897904
98	23.80888071728456	23.92209231701978	26.174112400585166	26.0949145651105
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	63.0
1	51.5
2	67.0
3	110.5
4	126.0
5	154.5
6	238.0
7	433.5
8	676.0
9	791.0
10	839.5
11	873.5
12	966.0
13	1151.0
14	1326.5
15	1479.0
16	1556.0
17	1656.5
18	1758.0
19	1769.5
20	1800.5
21	1920.5
22	2104.5
23	2380.0
24	2795.5
25	3395.5
26	4292.5
27	5524.5
28	7261.5
29	9858.5
30	13304.5
31	18327.0
32	22956.5
33	26852.0
34	31656.5
35	37173.5
36	43165.0
37	49063.5
38	55711.0
39	62552.5
40	69192.5
41	76477.0
42	84929.5
43	92014.0
44	97921.5
45	103849.0
46	110467.0
47	117395.5
48	121609.5
49	124033.5
50	125069.0
51	126903.5
52	128028.0
53	129748.5
54	128776.0
55	122870.5
56	114202.0
57	104093.5
58	95956.0
59	84577.0
60	72078.0
61	60785.5
62	49838.5
63	38459.0
64	28492.0
65	21830.5
66	16716.5
67	12929.0
68	9788.5
69	7581.5
70	5726.5
71	4277.0
72	3575.0
73	2900.0
74	2186.5
75	1640.5
76	1181.5
77	775.0
78	440.5
79	235.0
80	139.0
81	82.0
82	52.0
83	39.5
84	29.5
85	17.0
86	8.0
87	11.5
88	11.5
89	7.5
90	8.5
91	7.5
92	6.5
93	5.0
94	2.0
95	3.5
96	2.5
97	1.0
98	1.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3510477908335174
2	0.02176818529594886
3	0.01707800063514409
4	0.0148940215182808
5	0.02019285609689993
6	0.021983002914000987
7	0.012960662955811657
8	0.007518616631824442
9	0.0
10-11	3.580293634202116E-5
12-13	0.0048154949380018455
14-15	0.0027389246301646186
16-17	0.004833396406172856
18-19	0.033529449884302814
20-21	0.0704243757847556
22-23	0.08909560708711964
24-25	0.058681012664572665
26-27	0.06795397317715615
28-29	0.051681538609707534
30-31	0.04690184660804771
32-33	0.017525537339419354
34-35	0.3111454182803348
36-37	0.4746216256180034
38-39	0.43180131375294617
40-41	0.4405372302203993
42-43	0.3953718260249396
44-45	0.372332636488849
46-47	0.3699875441584466
48-49	0.36160965705441367
50-51	0.3581009692928956
52-53	0.41395354998644857
54-55	0.41363132355937043
56-57	0.3870476433254197
58-59	0.37324561136557055
60-61	0.3880501255429963
62-63	0.3698264309449075
64-65	0.4136492250275414
66-67	0.43307231799308793
68-69	0.38024508542043567
70-71	0.3414168009575137
72-73	0.32892157617414836
74-75	0.31041145808532344
76-77	0.30955218761311487
78-79	0.3091225523770107
80-81	0.32387336214992335
82-83	0.319899236215959
84-85	0.32040047732474736
86-87	0.3066342483012402
88-89	0.34712736930406607
90-91	0.32723883816607335
92-93	0.3184671187622781
94-95	0.32417768710883055
96-97	0.32480423849481593
98	0.3186461334439883
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	2793067.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.32434264157756
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.28364696231097	34.44735380547964
2	12.743206453591805	12.316141500314655
3	5.636184955328845	8.170947989178467
4	3.077845359651828	5.949394150304177
5	1.900566302239957	4.592180860123986
6	1.2256130584332163	3.5536167229031133
7	0.85523759569172	2.8930156229915953
8	0.619219446960155	2.3938698180184548
9	0.4752883344042249	2.0671196692765013
>10	2.0607073432278593	16.741804056840813
>50	0.08683927848196689	2.8541450491674714
>100	0.03288391101250082	2.7969177601247974
>500	0.00171629646734489	0.5206399206187692
>1k	0.0010447021974047408	0.7028530746575027
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	1.790146817101058E-5	0.0	3.580293634202116E-5	0.0
18-19	0.0	3.580293634202116E-5	0.0	5.370440451303174E-5	0.0
20-21	0.0	3.580293634202116E-5	0.0	7.160587268404232E-5	0.0
22-23	0.0	3.580293634202116E-5	0.0	1.4321174536808463E-4	0.0
24-25	0.0	3.580293634202116E-5	0.0	4.475367042752644E-4	0.0
26-27	0.0	3.580293634202116E-5	0.0	8.771719403795182E-4	0.0
28-29	0.0	3.580293634202116E-5	0.0	0.00341918042066302	0.0
30-31	0.0	3.580293634202116E-5	0.0	0.014356977473150484	0.0
32-33	0.0	3.580293634202116E-5	0.0	0.03800481692705546	0.0
34-35	0.0	3.580293634202116E-5	0.0	0.07196390204746252	0.0
36-37	0.0	3.580293634202116E-5	0.0	0.13230975125193917	0.0
38-39	0.0	3.580293634202116E-5	0.0	0.23592344902574838	0.0
40-41	0.0	3.580293634202116E-5	0.0	0.3563108224757945	0.0
42-43	0.0	3.580293634202116E-5	0.0	0.48108405562773826	0.0
44-45	0.0	3.580293634202116E-5	0.0	0.6353410068573364	0.0
46-47	0.0	3.580293634202116E-5	0.0	0.8248101459793123	0.0
48-49	0.0	3.580293634202116E-5	0.0	1.0330221222763365	0.0
50-51	0.0	3.580293634202116E-5	0.0	1.2637899484688337	0.0
52-53	3.580293634202116E-5	3.580293634202116E-5	0.0	1.5006442738394745	0.0
54-55	3.580293634202116E-5	3.580293634202116E-5	0.0	1.7705805123901432	0.0
56-57	3.580293634202116E-5	3.580293634202116E-5	0.0	2.0831043437196457	0.0
58-59	3.580293634202116E-5	3.580293634202116E-5	0.0	2.4180408131992537	0.0
60-61	3.580293634202116E-5	3.580293634202116E-5	0.0	2.7914654392465343	0.0
62-63	3.580293634202116E-5	3.580293634202116E-5	0.0	3.1719611452213643	0.0
64-65	3.580293634202116E-5	3.580293634202116E-5	0.0	3.5773756948902404	0.0
66-67	3.580293634202116E-5	3.580293634202116E-5	0.0	4.054807851011093	0.0
68-69	3.580293634202116E-5	3.580293634202116E-5	0.0	4.583653023719087	0.0
70-71	3.580293634202116E-5	3.580293634202116E-5	0.0	5.146099252184069	0.0
72-73	3.580293634202116E-5	3.580293634202116E-5	0.0	5.727037697269704	0.0
74-75	3.580293634202116E-5	3.580293634202116E-5	0.0	6.322798558000936	0.0
76-77	3.580293634202116E-5	3.580293634202116E-5	0.0	6.972979881972041	0.0
78-79	3.580293634202116E-5	3.580293634202116E-5	0.0	7.700173322014832	0.0
80-81	3.580293634202116E-5	3.580293634202116E-5	0.0	8.46555059366639	0.0
82-83	3.580293634202116E-5	3.580293634202116E-5	0.0	9.254271379812945	1.790146817101058E-5
84-85	3.580293634202116E-5	3.580293634202116E-5	0.0	10.045373061226243	3.580293634202116E-5
86	3.580293634202116E-5	3.580293634202116E-5	0.0	10.656708199266255	3.580293634202116E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2925	0.0	47.042397	1
GTATCAA	5475	0.0	43.28797	1
TCAACGC	6930	0.0	33.62638	4
ATCAACG	7090	0.0	32.86753	3
TATCAAC	7170	0.0	32.373432	2
CAACGCA	7190	0.0	32.346603	5
AACGCAG	7490	0.0	31.413416	6
ACGCAGA	8840	0.0	25.991192	7
GTACATG	11270	0.0	25.194479	1
CGCAGAG	9150	0.0	24.708765	8
TACATGG	11450	0.0	24.318714	2
ACATGGG	12010	0.0	22.34408	3
GCAGAGT	10330	0.0	21.486341	9
GTATAGG	670	0.0	20.605852	1
TATACCG	210	1.2039582E-6	19.659517	5
CATGGGG	8465	0.0	19.61694	4
GTGGTAT	1655	0.0	18.630346	1
GAGTACA	8195	0.0	18.250658	1
AGAGTAC	8850	0.0	18.136354	10-11
CAGAGTA	9565	0.0	17.787468	10-11
>>END_MODULE