FastQCFastQC Report
Mon 15 Aug 2016
SRR2920882_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920882_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4967649
Sequences flagged as poor quality0
Sequence length98
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA69200.043.2976231
GGTATCA39750.042.7246361
ATCAACG89300.033.0229033
TCAACGC89800.032.8390354
CAACGCA89550.032.8282855
AACGCAG96150.030.9514626
TATCAAC98450.030.3264122
GTACATG151700.027.0018521
TACATGG154800.026.041962
ACGCAGA113350.025.7250217
ACATGGG158000.024.9049643
CGCAGAG118800.024.157988
CATGGGG112900.021.8952834
GCAGAGT136000.020.057489
AGAGTAC118650.018.37350710-11
GAGTACA113450.018.2962111
GTATTAA24250.017.4608441
AGTACAT108550.017.3647172
GTGTAAG19250.016.7361851
CAGAGTA130300.016.06222210-11
Produced by FastQC (version 0.11.5)