##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920882_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4967649
Sequences flagged as poor quality	0
Sequence length	98
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.637937181149475	31.0	30.0	33.0	22.0	34.0
2	28.67699126890809	31.0	28.0	34.0	18.0	34.0
3	28.75423746726067	31.0	28.0	34.0	22.0	34.0
4	32.230532591976605	35.0	32.0	37.0	22.0	37.0
5	32.20225100444899	35.0	32.0	37.0	22.0	37.0
6	32.31996825862697	35.0	33.0	37.0	23.0	37.0
7	32.15420674850417	35.0	32.0	37.0	22.0	37.0
8	32.09257497862671	35.0	32.0	37.0	22.0	37.0
9	33.36925676512169	37.0	33.0	39.0	22.0	39.0
10-11	33.172298606443405	37.0	32.5	39.0	19.0	39.0
12-13	33.00838696534316	37.0	32.0	39.0	18.0	39.0
14-15	33.79060225470842	38.0	33.0	40.0	17.0	41.0
16-17	33.681855441074845	38.0	32.5	40.0	17.0	41.0
18-19	33.5641379855944	38.0	32.0	40.0	17.5	41.0
20-21	33.46638138081012	38.0	32.0	40.0	17.5	41.0
22-23	33.249772981142584	38.0	32.0	40.0	16.0	41.0
24-25	33.06450979125135	38.0	32.0	40.0	14.0	41.0
26-27	33.11869286658538	38.0	32.0	40.0	11.5	41.0
28-29	32.96504543698639	38.0	32.0	40.0	10.0	41.0
30-31	32.72606065766724	38.0	31.5	40.0	9.5	41.0
32-33	32.49432669256624	38.0	31.0	40.0	9.0	41.0
34-35	32.17199906837218	37.0	30.5	40.0	7.5	41.0
36-37	31.875793559488603	37.0	30.0	40.0	4.5	41.0
38-39	31.687827582021193	37.0	30.0	40.0	2.0	41.0
40-41	31.500893581652004	37.0	30.0	40.0	2.0	41.0
42-43	31.278595267097174	37.0	30.0	40.0	2.0	41.0
44-45	31.014207525531695	36.5	29.5	40.0	2.0	41.0
46-47	30.77922977247386	36.0	29.0	39.5	2.0	41.0
48-49	30.497360220096066	36.0	28.0	39.0	2.0	40.0
50-51	29.359922369716543	34.5	26.5	38.0	2.0	39.5
52-53	29.804983705571793	35.0	28.0	38.5	2.0	39.5
54-55	30.4610840057339	36.0	28.0	39.0	2.0	40.0
56-57	30.42183918388759	36.0	28.0	39.0	2.0	41.0
58-59	30.1877823896173	35.5	28.0	39.0	2.0	41.0
60-61	29.873539777065567	35.0	28.0	39.0	2.0	40.5
62-63	29.48860316016691	35.0	27.5	39.0	2.0	40.0
64-65	29.07688667214612	34.5	26.5	38.0	2.0	40.0
66-67	28.699842319777424	34.0	26.0	38.0	2.0	40.0
68-69	28.267853364841194	34.0	26.0	37.0	2.0	40.0
70-71	27.94271978555651	34.0	26.0	37.0	2.0	39.0
72-73	27.489259808814992	33.5	25.0	36.0	2.0	39.0
74-75	27.052482874695855	33.0	24.0	36.0	2.0	39.0
76-77	26.639675327302715	33.0	23.0	35.0	2.0	37.5
78-79	26.18431676634158	33.0	21.5	35.0	2.0	37.0
80-81	25.748046711834913	32.0	20.0	35.0	2.0	36.5
82-83	25.275560229798845	32.0	18.5	35.0	2.0	36.0
84-85	24.887266391003067	32.0	17.0	35.0	2.0	36.0
86-87	24.443103770012733	31.5	9.0	34.5	2.0	35.0
88-89	23.931540151085553	31.0	4.5	34.0	2.0	35.0
90-91	23.454707951387064	31.0	2.0	34.0	2.0	35.0
92-93	22.943697410988577	31.0	2.0	34.0	2.0	35.0
94-95	22.333204097149377	30.0	2.0	34.0	2.0	35.0
96-97	21.708043784897043	30.0	2.0	34.0	2.0	35.0
98	21.324143271797183	30.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	256192.0
3	43607.0
4	31529.0
5	25208.0
6	23266.0
7	23437.0
8	26988.0
9	31062.0
10	34244.0
11	36516.0
12	37903.0
13	37983.0
14	35885.0
15	36904.0
16	39823.0
17	42586.0
18	43683.0
19	43407.0
20	44976.0
21	48114.0
22	51877.0
23	58415.0
24	66458.0
25	76251.0
26	88500.0
27	102151.0
28	118559.0
29	137768.0
30	160488.0
31	189654.0
32	226206.0
33	275501.0
34	344434.0
35	440136.0
36	568326.0
37	651319.0
38	422038.0
39	46252.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.34609295842159	17.795778908607684	12.842012392246824	25.016115740723897
2	15.937025497992405	19.496229757181823	39.526066312539626	25.04067843228614
3	19.199703949010935	23.66024041659754	28.900850300963697	28.23920533342783
4	13.07926539540083	15.63317929744818	36.72727031123409	34.560284995916895
5	14.428688123711	36.83829979927688	34.29709558540014	14.435916491611971
6	33.72487691022465	35.0116792580364	17.373512960946115	13.889930870792835
7	29.644663489444838	31.194671512698385	20.97308676610132	18.187578231755452
8	26.65351620765887	34.604088037209436	19.990046560114127	18.752349195017562
9	27.01799181061303	15.654970792018519	19.272355997776817	38.05468139959164
10-11	25.624332241770375	25.27668277590147	27.78344276642012	21.31554221590803
12-13	26.36856939500633	22.490334130908245	27.599406886912227	23.541689587173202
14-15	23.427328197251317	24.114287554831733	24.991182634218223	27.467201613698723
16-17	23.8070298947382	27.074641120006344	25.601746934821513	23.516582050433946
18-19	23.701777106426015	26.382489801680265	26.89621688000388	23.01951621188984
20-21	24.463667729446136	25.733805372348208	26.608896080636395	23.193630817569264
22-23	24.18557139139452	25.562859849013407	26.36231442654322	23.889254333048846
24-25	23.87223794695855	25.71629746633053	26.505959783059406	23.905504803651517
26-27	23.725852889675718	25.983098533667153	26.376615951657833	23.914432624999296
28-29	23.820890032989286	26.05948263507838	26.387885014754392	23.731742317177947
30-31	24.003612506693138	25.802655341463556	26.24881079425701	23.9449213575863
32-33	23.779392626628738	25.878256531032047	26.22268625384832	24.119664588490895
34-35	24.167596323225983	25.90482700308418	26.141760657419184	23.785816016270655
36-37	24.193172406578256	25.72283184845071	26.10157151566892	23.98242422930211
38-39	24.058095329785463	25.85312358925153	26.164145590050857	23.92463549091215
40-41	24.19866875726236	25.61711292078961	26.293980866457723	23.890237455490308
42-43	24.06713581460947	25.576555434072368	26.179959371421546	24.176349379896617
44-45	23.96527164748964	25.720355105590297	26.18058359276348	24.133789654156583
46-47	23.993471582181257	25.72464427197025	26.080574419920772	24.20130972592772
48-49	24.019506299835594	25.65520008002999	26.134763633644525	24.190529986489885
50-51	23.86111172580288	25.858169491032186	26.185646950621926	24.09507183254301
52-53	23.934508407619717	25.729928926110496	26.222530350176616	24.11303231609318
54-55	23.67932087577986	25.55750863085714	26.418132357840467	24.345038135522532
56-57	23.669775495207375	25.660389373446556	26.448688156566092	24.221146974779977
58-59	23.6777541558377	25.43821191750458	26.46928332543016	24.41475060122756
60-61	23.667477916045293	25.474776977175246	26.571641527639382	24.286103579140075
62-63	23.558737044476786	25.504388599958144	26.58885327039704	24.348021085168035
64-65	23.46039175909816	25.327463498557414	26.554379890012385	24.65776485233204
66-67	23.438047990796484	25.424487449734013	26.455787167099697	24.681677392369807
68-69	23.58308191237664	25.221574035508244	26.530950727489326	24.664393324625784
70-71	23.389507635412816	25.12885646260407	26.57943184248552	24.902204059497596
72-73	23.427469009693034	25.125474896485166	26.597779003168654	24.849277090653143
74-75	23.39239178813814	25.26378757499041	26.52424149110538	24.81957914576607
76-77	23.43719733628664	25.07936543203942	26.642152946786936	24.841284284887006
78-79	23.35781174066744	25.07465318384922	26.692977066364175	24.87455800911916
80-81	23.43998971324665	25.11032452060386	26.69710470923003	24.75258105691946
82-83	23.467871766443032	25.055100246035817	26.65203521688756	24.824992770633592
84-85	23.426430254880067	24.967340290637576	26.69269410331397	24.913535351168388
86-87	23.380861143899125	24.918773919429547	26.785216550366837	24.91514838630449
88-89	23.399312628255796	24.740745350864813	26.83259476114706	25.027347259732334
90-91	23.298237504825785	24.77717546224008	26.952814471034277	24.971772561899858
92-93	23.466750187710247	24.58687080707225	26.928234186434402	25.018144818783107
94-95	23.393415645508433	24.49274741631378	27.01890952461951	25.094927413558278
96-97	23.50261308380252	24.317305341923078	26.987297200656958	25.19278437361745
98	23.48889167208717	24.215973044275746	26.887324911016215	25.40781037262087
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	53.0
1	55.0
2	99.5
3	154.0
4	175.0
5	208.0
6	334.5
7	623.0
8	943.5
9	1156.5
10	1279.0
11	1392.0
12	1613.5
13	1949.0
14	2149.0
15	2322.0
16	2547.0
17	2633.0
18	2663.5
19	2644.5
20	2617.5
21	2671.0
22	2888.5
23	3354.5
24	4109.5
25	5197.5
26	6675.0
27	8902.0
28	12030.0
29	16640.0
30	23607.0
31	33231.5
32	43821.5
33	54773.0
34	67104.0
35	81791.0
36	97460.0
37	111600.0
38	124675.0
39	138005.0
40	150834.5
41	162344.0
42	174470.5
43	186628.0
44	196950.0
45	205540.0
46	212233.0
47	220265.5
48	226019.5
49	224786.5
50	223080.0
51	221515.5
52	218475.0
53	215591.5
54	207595.5
55	194836.0
56	178781.5
57	160270.0
58	143309.0
59	124472.0
60	104509.5
61	86506.0
62	70864.5
63	55275.5
64	40610.0
65	29989.5
66	22578.0
67	17116.5
68	12443.0
69	9163.0
70	6975.5
71	5569.0
72	4609.0
73	3462.5
74	2487.5
75	1673.0
76	1087.5
77	645.0
78	359.0
79	214.5
80	130.5
81	89.5
82	57.5
83	43.5
84	32.5
85	23.5
86	19.0
87	16.0
88	13.5
89	10.0
90	7.0
91	5.5
92	5.0
93	4.0
94	4.0
95	3.0
96	1.5
97	1.5
98	3.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3839039352418015
2	0.025323850376707372
3	0.0188620411788353
4	0.016929537493490382
5	0.02226405287491125
6	0.023190044224139023
7	0.01682888625987867
8	0.010507988789062997
9	0.0
10-11	3.0195370083514355E-5
12-13	0.006029008893341699
14-15	0.00360331416329938
16-17	0.0064416789511497295
18-19	0.03784486383800466
20-21	0.07921252085241932
22-23	0.09844697159561797
24-25	0.06539310647753092
26-27	0.07450204311939108
28-29	0.05748191951565016
30-31	0.05385847510562844
32-33	0.02080460998754139
34-35	0.3401206486207057
36-37	0.5179915086593276
38-39	0.4723562393397762
40-41	0.48156582721524804
42-43	0.43032428418352425
44-45	0.4047488057227876
46-47	0.40359131653625285
48-49	0.39262033207257596
50-51	0.38991281388842086
52-53	0.4503740099189778
54-55	0.4520548855202934
56-57	0.42241309722164344
58-59	0.408060231308613
60-61	0.42188971080686255
62-63	0.40261499957021923
64-65	0.450212967945199
66-67	0.4720744158856634
68-69	0.41469314760362497
70-71	0.3712118146833643
72-73	0.35740246543183707
74-75	0.3408554026260712
76-77	0.33851022888291826
78-79	0.33728228383285536
80-81	0.35416149570953986
82-83	0.34933023649617756
84-85	0.34946108309987284
86-87	0.33522899866717637
88-89	0.3784184430099631
90-91	0.3558625015575778
92-93	0.34624024362429795
94-95	0.3533059602238403
96-97	0.353467002197619
98	0.3484344405170333
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	4967649.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.97329540025512
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.65035367005959	39.54475236697586
2	12.300531584243345	13.031993863845818
3	4.9940018640146615	7.9364620791562
4	2.5206091432583446	5.34099890937633
5	1.448574716214645	3.8367888175689555
6	0.9349017469058885	2.9714895845436105
7	0.6475617501462764	2.4012435916283428
8	0.43672539598099935	1.8507826728075893
9	0.3317047004700267	1.581434197528668
>10	1.6126908129779742	14.99292600524662
>50	0.08989285543146093	3.2328696638009933
>100	0.03142015449801588	2.7970564058199434
>500	8.023604036144585E-4	0.2999948520848374
>1k	2.2924539513274325E-4	0.18120698961627918
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.0130246722342903E-5	0.0	0.0	0.0	0.0
3	2.0130246722342903E-5	0.0	0.0	0.0	0.0
4	2.0130246722342903E-5	0.0	0.0	2.0130246722342903E-5	0.0
5	4.0260493444685805E-5	0.0	0.0	2.0130246722342903E-5	0.0
6	4.0260493444685805E-5	0.0	0.0	2.0130246722342903E-5	0.0
7	4.0260493444685805E-5	0.0	0.0	2.0130246722342903E-5	0.0
8	6.039074016702871E-5	0.0	0.0	2.0130246722342903E-5	0.0
9	6.039074016702871E-5	0.0	0.0	4.0260493444685805E-5	0.0
10-11	8.052098688937161E-5	0.0	0.0	4.0260493444685805E-5	1.0065123361171451E-5
12-13	1.0065123361171451E-4	0.0	0.0	1.2078148033405742E-4	1.6104197377874322E-4
14-15	1.4091172705640032E-4	0.0	0.0	1.4091172705640032E-4	2.6169320739045774E-4
16-17	1.5097685041757177E-4	0.0	0.0	2.2143271394577194E-4	3.321490709186579E-4
18-19	2.2143271394577194E-4	0.0	0.0	3.12018824196315E-4	3.724095643633437E-4
20-21	2.717583307516292E-4	0.0	0.0	4.026049344468581E-4	4.026049344468581E-4
22-23	3.0195370083514354E-4	0.0	0.0	5.435166615032584E-4	4.7306079797505824E-4
24-25	3.724095643633437E-4	0.0	0.0	0.0010165774594783166	5.032561680585726E-4
26-27	3.8247468772451514E-4	0.0	0.0	0.0015097685041757175	5.032561680585726E-4
28-29	4.126700578080295E-4	0.0	0.0	0.004499110142443639	5.032561680585726E-4
30-31	4.428654278915439E-4	0.0	0.0	0.02191177355727025	5.032561680585726E-4
32-33	4.629956746138868E-4	0.0	0.0	0.06066249849778034	5.13321291419744E-4
34-35	5.032561680585726E-4	0.0	0.0	0.10969977951340765	5.636469082256013E-4
36-37	5.837771549479442E-4	0.0	0.0	0.19195196762090078	5.636469082256013E-4
38-39	6.642981418373158E-4	0.0	0.0	0.3353699103942328	5.636469082256013E-4
40-41	6.642981418373158E-4	0.0	0.0	0.5018873112814533	5.737120315867728E-4
42-43	7.14623758643173E-4	0.0	0.0	0.6790536126847931	5.837771549479442E-4
44-45	7.649493754490303E-4	0.0	0.0	0.8906325708599783	5.938422783091156E-4
46-47	0.0010065123361171452	0.0	0.0	1.142341175876154	6.139725250314586E-4
48-49	0.0010870333230065168	0.0	0.0	1.4176222998041932	6.441678951149729E-4
50-51	0.0010870333230065168	0.0	0.0	1.7193243725553073	6.642981418373158E-4
52-53	0.0010970984463676882	0.0	0.0	2.0291691301056094	7.548842520878588E-4
54-55	0.0011071635697288596	0.0	0.0	2.371685278086274	8.052098688937161E-4
56-57	0.0011071635697288596	0.0	0.0	2.7677277521016483	8.052098688937161E-4
58-59	0.0011071635697288596	0.0	0.0	3.1899294817327073	8.052098688937161E-4
60-61	0.0011071635697288596	0.0	0.0	3.6502176381624385	8.25340115616059E-4
62-63	0.0011474240631735456	0.0	0.0	4.11522633744856	9.058611025054307E-4
64-65	0.0011675543098958884	0.0	0.0	4.611869719458843	9.461215959501165E-4
66-67	0.0012883357902299458	0.0	0.0	5.171742206424005	9.461215959501165E-4
68-69	0.0012883357902299458	0.0	0.0	5.7868218950251915	9.56186719311288E-4
70-71	0.0013285962836746316	0.0	0.0	6.443460477984656	0.0010165774594783166
72-73	0.001358791653758146	0.0	0.0	7.109791774740929	0.0010870333230065168
74-75	0.0013889870238416604	0.0	0.0	7.795075698786287	0.0011071635697288596
76-77	0.0013889870238416604	0.0	0.0	8.530554393033807	0.0011272938164512026
78-79	0.0013990521472028318	0.0	0.0	9.337998719313703	0.001137358939812374
80-81	0.0015701592443427463	0.0	0.0	10.17964433477486	0.0011474240631735456
82-83	0.0016104197377874321	0.0	2.0130246722342903E-5	11.03781688279506	0.0012078148033405742
84-85	0.0016204848611486035	1.0065123361171451E-5	2.0130246722342903E-5	11.898143367214551	0.0012078148033405742
86	0.0016305499845097752	2.0130246722342903E-5	2.0130246722342903E-5	12.56093174054769	0.0012078148033405742
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6920	0.0	43.297623	1
GGTATCA	3975	0.0	42.724636	1
ATCAACG	8930	0.0	33.022903	3
TCAACGC	8980	0.0	32.839035	4
CAACGCA	8955	0.0	32.828285	5
AACGCAG	9615	0.0	30.951462	6
TATCAAC	9845	0.0	30.326412	2
GTACATG	15170	0.0	27.001852	1
TACATGG	15480	0.0	26.04196	2
ACGCAGA	11335	0.0	25.725021	7
ACATGGG	15800	0.0	24.904964	3
CGCAGAG	11880	0.0	24.15798	8
CATGGGG	11290	0.0	21.895283	4
GCAGAGT	13600	0.0	20.05748	9
AGAGTAC	11865	0.0	18.373507	10-11
GAGTACA	11345	0.0	18.296211	1
GTATTAA	2425	0.0	17.460844	1
AGTACAT	10855	0.0	17.364717	2
GTGTAAG	1925	0.0	16.736185	1
CAGAGTA	13030	0.0	16.062222	10-11
>>END_MODULE