FastQCFastQC Report
Mon 15 Aug 2016
SRR2920882_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920882_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4967649
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA69050.043.3808481
GGTATCA39650.043.1698461
TCAACGC87400.033.728074
ATCAACG88100.033.4600833
CAACGCA88950.032.7631455
TATCAAC95600.030.9328252
AACGCAG95350.030.9124956
GTACATG145050.026.3220081
ACGCAGA112100.025.74267
TACATGG147800.025.1464562
CGCAGAG117050.024.246758
ACATGGG155000.023.642693
GCAGAGT138000.019.809019
CATGGGG108400.019.7934024
AGAGTAC116400.018.79437610-11
GAGTACA113400.017.4841371
AGTACAT107050.017.4702432
GTATATA18550.017.1732331
CAGAGTA130950.015.72766410-11
GTATAGC16750.015.3285171
Produced by FastQC (version 0.11.5)