##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920882_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4967649
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.26270515489319	31.0	30.0	34.0	27.0	34.0
2	30.28147540214697	31.0	30.0	34.0	26.0	34.0
3	30.422777454687317	31.0	30.0	34.0	27.0	34.0
4	34.060961030056674	35.0	35.0	37.0	30.0	37.0
5	33.576662119243935	35.0	35.0	37.0	28.0	37.0
6	33.51416776829442	35.0	35.0	37.0	28.0	37.0
7	33.3254157046925	35.0	35.0	37.0	27.0	37.0
8	33.261033740507834	35.0	35.0	37.0	27.0	37.0
9	34.59847646240706	38.0	35.0	39.0	27.0	39.0
10-11	34.40774096559559	38.0	34.0	39.0	27.0	39.0
12-13	34.229770158882	37.0	34.0	39.0	26.0	39.0
14-15	35.14302198082031	38.5	34.0	40.0	26.0	41.0
16-17	35.02052178002109	38.0	34.0	40.0	25.5	41.0
18-19	34.92757600224975	38.5	34.0	40.0	25.0	41.0
20-21	34.80948422483151	38.5	34.0	40.0	25.0	41.0
22-23	34.664874672103444	38.0	34.0	40.0	24.5	41.0
24-25	34.49391432446214	38.0	33.5	40.0	24.0	41.0
26-27	34.3192445762573	38.0	33.0	40.0	23.0	41.0
28-29	34.26336915108132	38.0	33.0	40.0	23.5	41.0
30-31	34.26195188106084	38.0	33.0	40.0	23.0	41.0
32-33	34.12435308935877	38.0	33.0	40.0	22.0	41.0
34-35	33.96635893558502	38.0	33.0	40.0	21.5	41.0
36-37	33.78196607691083	38.0	33.0	40.0	19.5	41.0
38-39	33.605183961266185	38.0	33.0	40.0	18.5	41.0
40-41	33.382060004642035	38.0	32.0	40.0	17.0	41.0
42-43	33.41668694789024	38.0	32.0	40.0	17.0	41.0
44-45	33.28991863152972	38.0	32.0	40.0	15.5	41.0
46-47	33.06174369404924	38.0	32.0	40.0	14.5	41.0
48-49	32.80779902122714	37.5	31.5	40.0	12.5	41.0
50-51	32.564288056583706	37.0	31.0	40.0	9.0	41.0
52-53	32.30885787220474	37.0	31.0	40.0	8.0	41.0
54-55	32.035300702606	37.0	31.0	40.0	7.5	41.0
56-57	31.717971619975565	36.0	30.0	39.5	6.5	41.0
58-59	31.39651915825776	36.0	30.0	39.0	2.0	41.0
60-61	31.071921647443286	35.5	29.0	39.0	2.0	40.0
62-63	30.75977036622354	35.0	29.0	39.0	2.0	40.0
64-65	30.359284643500377	35.0	29.0	38.5	2.0	40.0
66-67	29.93688322182183	34.5	28.0	38.0	2.0	40.0
68-69	29.50049631123294	34.0	28.0	37.5	2.0	40.0
70-71	29.07741891586946	34.0	27.5	37.0	2.0	39.0
72-73	28.6345726117123	34.0	26.0	36.0	2.0	39.0
74-75	28.1821176375384	33.5	26.0	36.0	2.0	39.0
76-77	26.71983165477271	31.5	25.0	34.5	2.0	36.5
78-79	27.56221615093981	33.0	26.0	35.0	2.0	37.0
80-81	27.5834964386574	33.0	26.0	35.0	2.0	37.0
82-83	27.24454857821074	33.0	26.0	35.0	2.0	36.0
84-85	26.918598314816524	33.0	25.5	35.0	2.0	36.0
86-87	26.38244328453963	33.0	24.0	35.0	2.0	35.5
88-89	26.016790538139873	32.5	23.5	35.0	2.0	35.0
90-91	25.658745414581425	32.0	22.0	35.0	2.0	35.0
92-93	25.354393798756718	32.0	20.0	35.0	2.0	35.0
94-95	24.97491902105	32.0	18.5	34.0	2.0	35.0
96-97	24.459794160175164	32.0	11.5	34.0	2.0	35.0
98-99	23.881247648535556	32.0	2.0	34.0	2.0	35.0
100-101	21.688674360849568	28.0	2.0	33.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	122223.0
3	54873.0
4	28660.0
5	20097.0
6	16931.0
7	16394.0
8	18771.0
9	21464.0
10	24661.0
11	27469.0
12	30707.0
13	33290.0
14	35740.0
15	38101.0
16	39961.0
17	41397.0
18	40783.0
19	39172.0
20	40020.0
21	41428.0
22	44480.0
23	50721.0
24	58849.0
25	69771.0
26	83204.0
27	98486.0
28	116199.0
29	137535.0
30	162833.0
31	192368.0
32	231371.0
33	282533.0
34	357417.0
35	465398.0
36	626815.0
37	759313.0
38	463268.0
39	34945.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.42297302366707	18.97433301372797	13.510593969825328	25.092099992779637
2	15.99625899494912	20.513969485364203	38.296345011493365	25.193426508193312
3	18.91954629746511	24.44126213523821	29.1491738646112	27.49001770268548
4	13.485809036804719	16.508700726535277	36.263727856599125	33.74176238006088
5	14.41850240129873	37.110090930467024	34.26533304129074	14.206073626943505
6	33.757575305687375	35.46424963540393	17.085464300014852	13.692710758893837
7	29.55645590483624	31.337147699929684	20.96012050291258	18.146275892321487
8	26.681088328209228	34.84610959790935	19.85888189864473	18.613920175236693
9	26.888849332526704	15.509458282339562	19.18641253742259	38.41527984771114
10-11	25.62279183010138	25.36080924293995	27.833899069543133	21.182499857415532
12-13	26.344015322200416	22.540077737575174	27.65670475753436	23.459202182690053
14-15	23.336607641121894	24.17121351395635	24.998090434983794	27.494088409937962
16-17	23.80130230924159	27.183748962978026	25.62585667306329	23.389092054717093
18-19	23.699106087153428	26.474474699532585	26.86400400670228	22.962415206611702
20-21	24.450724051061158	25.76599789359641	26.59515768827241	23.188120367070024
22-23	24.103868039120204	25.606727630724546	26.4025517626677	23.88685256748755
24-25	23.8577139918744	25.76474836967348	26.520991870170878	23.85654576828124
26-27	23.74106574073841	26.04506598246007	26.355315736332024	23.858552540469493
28-29	23.819412237010287	26.076210503336828	26.398404556430883	23.705972703222
30-31	23.97790926290951	25.870517940921495	26.254657398968334	23.89691539720066
32-33	23.78089324038764	25.94223703568266	26.169872498365905	24.1069972255638
34-35	24.098324017847077	25.957870914743165	26.15185517774592	23.791949889663837
36-37	24.19333225878646	25.809451428383884	26.100696586253175	23.896519726576482
38-39	24.03518435864099	25.875684792238278	26.194038739524167	23.89509210959656
40-41	24.11622378899491	25.685428373038306	26.300924321160906	23.89742351680588
42-43	24.015160849477873	25.660971885667898	26.181980123229216	24.141887141625013
44-45	23.898360867327096	25.727845925213572	26.22421994736339	24.14957326009594
46-47	23.88878093180278	25.800936978009307	26.101130394596016	24.209151695591892
48-49	23.89578188999054	25.725672614943946	26.20640269493913	24.17214280012639
50-51	23.81248581226334	25.84976174669095	26.188294355961062	24.149458085084643
52-53	23.941124827195654	25.74227607103464	26.138173418559656	24.17842568321005
54-55	23.63077826881499	25.62679722337482	26.30811890469107	24.43430560311912
56-57	23.604081096092496	25.720564376752304	26.297235309239802	24.3781192179154
58-59	23.581601991278646	25.526841023327506	26.33100427653038	24.56055270886347
60-61	23.644309782248126	25.55123561323801	26.405901619077333	24.398552985436528
62-63	23.47716488447128	25.622020230347776	26.356496991277577	24.544317893903365
64-65	23.36411458113144	25.42907529427396	26.290124836178936	24.91668528841566
66-67	23.339789382107295	25.56091853446518	26.20026371289429	24.89902837053323
68-69	23.448929918019534	25.39267804233745	26.245717349984975	24.912674689658044
70-71	23.251004342545112	25.26201498724217	26.237086754759208	25.24989391545351
72-73	23.295055824262402	25.278783980943516	26.148926929762737	25.277233265031345
74-75	23.148234801567604	25.370396357068607	26.06675853309614	25.414610308267648
76-77	23.150748305497327	25.174798009227377	26.295634274989972	25.378819410285324
78-79	23.08344434048856	25.144480546953	26.273620751206174	25.498454361352263
80-81	23.031605371169526	25.24261025276968	26.20786685388259	25.5179175221782
82-83	23.004787228206244	25.198746424074308	26.181911450622657	25.61455489709679
84-85	22.881240253143456	25.034074990346255	26.164609684826978	25.920075071683314
86-87	22.80912741718903	25.029129689839696	26.23941708730551	25.922325805665764
88-89	22.761201172768175	24.903082137426413	26.24920098154056	26.08651570826485
90-91	22.585149243993055	24.915414157013327	26.26528562690107	26.23415097209255
92-93	22.625358159676942	24.74300281800428	26.27797574807216	26.353663274246625
94-95	22.419486563597175	24.615321281741014	26.378632120584903	26.586560034076907
96-97	22.44986721007281	24.467307809416635	26.402361783476675	26.680463197033884
98-99	22.26494790063007	24.41473073310735	26.349917770152402	26.97040359611018
100-101	22.306972845264227	24.217213728594366	26.506950137134556	26.96886328900685
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	51.0
1	41.0
2	59.5
3	94.0
4	89.0
5	84.5
6	165.5
7	379.0
8	570.5
9	600.0
10	528.0
11	481.5
12	516.5
13	559.0
14	617.0
15	686.5
16	775.0
17	895.0
18	1042.0
19	1175.0
20	1347.5
21	1627.0
22	2017.0
23	2481.5
24	3031.0
25	3890.5
26	5124.5
27	6900.5
28	9324.0
29	12997.5
30	18454.0
31	25968.0
32	36017.0
33	46453.5
34	56535.5
35	68347.0
36	83013.0
37	97580.0
38	110216.0
39	122981.0
40	135904.0
41	148585.0
42	160897.5
43	173092.5
44	184172.5
45	194062.5
46	202634.5
47	210325.0
48	218461.5
49	223729.5
50	222694.0
51	219988.5
52	217537.5
53	215627.5
54	210559.0
55	197546.5
56	180463.0
57	162983.0
58	146840.0
59	127914.0
60	107528.0
61	89479.5
62	72882.5
63	56408.5
64	41820.0
65	31084.5
66	23051.5
67	17371.0
68	12776.0
69	9118.0
70	6951.5
71	5750.5
72	4833.5
73	3748.0
74	2635.5
75	1812.0
76	1190.5
77	699.0
78	369.0
79	186.0
80	105.0
81	59.5
82	31.5
83	20.5
84	11.5
85	5.5
86	2.5
87	4.0
88	3.0
89	1.5
90	1.0
91	0.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.19025096177286277
2	0.0
3	0.012198929513739798
4	0.013084660369522888
5	0.006783893145429558
6	0.10685134960219612
7	0.14302540296224633
8	0.12341854265468435
9	0.14244162580729838
10-11	0.11429954088946301
12-13	0.11471221094727103
14-15	0.11647360753547605
16-17	0.0667015725144832
18-19	0.07983655850081195
20-21	0.0370195237223886
22-23	0.08410417080594865
24-25	0.057290682171787906
26-27	0.06565479968492138
28-29	0.04042153541846455
30-31	0.024629356864786543
32-33	0.03210774352213693
34-35	0.02031141894284399
36-37	0.024448184644285456
38-39	0.03292301851439182
40-41	0.025112482786122773
42-43	0.012984009135911172
44-45	0.02684368400424426
46-47	0.03389933548042545
48-49	0.008303726772966447
50-51	0.01553048534628755
52-53	0.006562460431483787
54-55	0.012319710994073856
56-57	0.005193603654364469
58-59	0.0037744212604392943
60-61	0.023099458113888482
62-63	0.010216100211589023
64-65	0.006824153638874244
66-67	0.04069329374921618
68-69	0.08967018402467646
70-71	0.02197216429743728
72-73	0.04429660791251556
74-75	0.08622791183515582
76-77	0.2473302763540661
78-79	0.08062163812298333
80-81	0.016808756013156324
82-83	0.011645347728875368
84-85	0.013346353576913344
86-87	0.26334388762168986
88-89	0.6265237338628393
90-91	0.26899042182730704
92-93	0.35614432501169063
94-95	0.00124807529678526
96-97	0.004358198415387238
98-99	0.003432207066159465
100-101	0.0053345153814208695
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4967649.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.109041616880454
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.03559310052722	36.096345325709855
2	13.387962658289364	13.417159560189267
3	5.579860478776373	8.388043828421754
4	2.7865728314610925	5.585299759206092
5	1.6464278949189064	4.125046195284218
6	1.0398371793683787	3.1263146697449806
7	0.7008001404342851	2.4581496381496084
8	0.502373590062114	2.0138767305315293
9	0.35307715034413195	1.5923121858507299
>10	1.8268219554568432	16.088723237407603
>50	0.10106354560302375	3.433473749223607
>100	0.03831791882367824	3.1486480806973223
>500	0.0010090286383417986	0.3371638530954559
>1k	2.8252729622880795E-4	0.1894431864880308
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0130246722342903E-5
2	0.0	0.0	0.0	0.0	2.0130246722342903E-5
3	0.0	0.0	0.0	0.0	2.0130246722342903E-5
4	0.0	0.0	0.0	6.039074016702871E-5	2.0130246722342903E-5
5	0.0	0.0	0.0	1.0065123361171451E-4	2.0130246722342903E-5
6	2.0130246722342903E-5	0.0	0.0	1.0065123361171451E-4	4.0260493444685805E-5
7	1.2078148033405742E-4	0.0	0.0	1.4091172705640032E-4	4.0260493444685805E-5
8	1.4091172705640032E-4	0.0	0.0	1.4091172705640032E-4	4.0260493444685805E-5
9	1.4091172705640032E-4	0.0	0.0	1.6104197377874322E-4	4.0260493444685805E-5
10-11	1.6104197377874322E-4	0.0	0.0	1.8117222050108612E-4	4.0260493444685805E-5
12-13	1.6104197377874322E-4	0.0	0.0	2.2143271394577194E-4	2.2143271394577194E-4
14-15	2.2143271394577194E-4	0.0	0.0	2.2143271394577194E-4	2.8182345411280064E-4
16-17	2.2143271394577194E-4	0.0	0.0	2.8182345411280064E-4	3.6234444100217224E-4
18-19	2.6169320739045774E-4	0.0	0.0	3.522793176410008E-4	3.6234444100217224E-4
20-21	3.12018824196315E-4	0.0	0.0	4.328003045303724E-4	3.6234444100217224E-4
22-23	3.2208394755748644E-4	0.0	0.0	5.938422783091156E-4	3.8247468772451514E-4
24-25	4.3280030453037244E-4	0.0	0.0	9.964472127559736E-4	3.8247468772451514E-4
26-27	4.629956746138868E-4	0.0	0.0	0.0013889870238416604	4.126700578080295E-4
28-29	5.636469082256013E-4	0.0	0.0	0.00443871940227661	4.3280030453037244E-4
30-31	6.039074016702871E-4	1.0065123361171451E-5	0.0	0.02191177355727025	4.629956746138868E-4
32-33	6.341027717538014E-4	2.0130246722342903E-5	0.0	0.06067256362114151	4.931910446974012E-4
34-35	6.844283885596587E-4	2.0130246722342903E-5	0.0	0.10969977951340765	5.233864147809155E-4
36-37	8.555354856995734E-4	2.0130246722342903E-5	0.0	0.19163994879670443	5.233864147809155E-4
38-39	9.259913492277736E-4	2.0130246722342903E-5	0.0	0.3347056122523955	5.233864147809155E-4
40-41	9.259913492277736E-4	2.0130246722342903E-5	0.0	0.5028032375073199	5.233864147809155E-4
42-43	0.0010065123361171452	2.0130246722342903E-5	0.0	0.6822643870370069	5.233864147809155E-4
44-45	0.0010669030762841738	2.0130246722342903E-5	0.0	0.8961885189553449	5.636469082256013E-4
46-47	0.0011977496799794028	2.0130246722342903E-5	0.0	1.1495276739560303	6.2403764839263E-4
48-49	0.001268205543507603	2.0130246722342903E-5	0.0	1.4236009830807288	6.743632651984872E-4
50-51	0.001268205543507603	2.0130246722342903E-5	0.0	1.7226961888813	7.246888820043445E-4
52-53	0.001268205543507603	2.0130246722342903E-5	0.0	2.028857111281413	8.25340115616059E-4
54-55	0.0012883357902299458	2.0130246722342903E-5	0.0	2.3685952852143943	9.058611025054307E-4
56-57	0.0013487265303969746	2.0130246722342903E-5	0.0	2.7609337938328573	9.461215959501165E-4
58-59	0.0013487265303969746	2.0130246722342903E-5	0.0	3.18153516884949	9.461215959501165E-4
60-61	0.001358791653758146	2.0130246722342903E-5	0.0	3.63897489536801	9.461215959501165E-4
62-63	0.0013889870238416604	2.0130246722342903E-5	0.0	4.101477378937199	0.001026642582839488
64-65	0.0014191823939251748	2.0130246722342903E-5	0.0	4.594809335361657	0.0010367077062006596
66-67	0.0015500289976204036	2.0130246722342903E-5	0.0	5.148048906031807	0.0010568379529230024
68-69	0.0015902894910650894	2.0130246722342903E-5	0.0	5.755006040080529	0.0010669030762841738
70-71	0.0016104197377874324	2.0130246722342903E-5	0.0	6.4050922277318705	0.0010870333230065168
72-73	0.0016305499845097752	2.0130246722342903E-5	0.0	7.066592265274781	0.001157489186534717
74-75	0.0016406151078709465	2.0130246722342903E-5	0.0	7.749249192122873	0.0011977496799794028
76-77	0.001650680231232118	2.0130246722342903E-5	0.0	8.484224630202334	0.00124807529678526
78-79	0.001650680231232118	2.0130246722342903E-5	0.0	9.287864339851708	0.001268205543507603
80-81	0.0017714617115661756	2.0130246722342903E-5	0.0	10.124094918944554	0.0012782706668687744
82-83	0.0017714617115661756	2.0130246722342903E-5	0.0	10.980918740434358	0.0013185311603134602
84-85	0.0018419175750943755	2.0130246722342903E-5	0.0	11.839081223331199	0.0013285962836746316
86-87	0.0019224385619837471	2.0130246722342903E-5	0.0	12.729200472899755	0.0013889870238416604
88-89	0.0019325036853449187	2.0130246722342903E-5	0.0	13.672755462392773	0.0014292475172863462
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6905	0.0	43.380848	1
GGTATCA	3965	0.0	43.169846	1
TCAACGC	8740	0.0	33.72807	4
ATCAACG	8810	0.0	33.460083	3
CAACGCA	8895	0.0	32.763145	5
TATCAAC	9560	0.0	30.932825	2
AACGCAG	9535	0.0	30.912495	6
GTACATG	14505	0.0	26.322008	1
ACGCAGA	11210	0.0	25.7426	7
TACATGG	14780	0.0	25.146456	2
CGCAGAG	11705	0.0	24.24675	8
ACATGGG	15500	0.0	23.64269	3
GCAGAGT	13800	0.0	19.80901	9
CATGGGG	10840	0.0	19.793402	4
AGAGTAC	11640	0.0	18.794376	10-11
GAGTACA	11340	0.0	17.484137	1
AGTACAT	10705	0.0	17.470243	2
GTATATA	1855	0.0	17.173233	1
CAGAGTA	13095	0.0	15.727664	10-11
GTATAGC	1675	0.0	15.328517	1
>>END_MODULE